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The map label for this gene is prfC

Identifier: 34497841

GI number: 34497841

Start: 2567819

End: 2569429

Strand: Reverse

Name: prfC

Synonym: CV_2386

Alternate gene names: 34497841

Gene position: 2569429-2567819 (Counterclockwise)

Preceding gene: 34497850

Following gene: 34497840

Centisome position: 54.08

GC content: 62.88

Gene sequence:

>1611_bases
ATGTCCGCCGATCTCGCCCGCATCGCAGAAGAAGTTTCGCATCGCCGCACCTTCGCCATCATTTCCCACCCCGACGCGGG
CAAAACCACGCTGACGGAAAAACTGTTGCTGTTCTCCGGCGCCATCCAGATGGCCGGCACGGTGAAAGGCAAGAAGGGCG
GCAAGTTCGCCACTTCCGACTGGATGGAGATCGAGAAGCAGCGCGGCATTTCCGTCGCGTCGTCGGTGATGCAGTTCGAC
TACCGCGACCACACCGTCAACCTGCTGGACACCCCCGGCCACCAGGACTTCTCCGAGGACACCTACCGCGTGCTGACCGC
CGTCGACAGCGCGCTGATGGTGATCGACGCCGCGAAAGGCGTGGAGGAGCAGACCATCAAGCTGTTGAACGTCTGCCGCC
TGCGCAACACGCCCATCGTCACCTTCATGAACAAGTGCGACCGCGAAGTCCGCGACAGCCTGGAATTGCTGGATGAAGTG
GAAAACGTGCTGAAGATCCGCTGCGCGCCGATCACCTGGCCGATCGGCATGGGCAAAACCTTCCGCGGCGTATACAGCCT
GCTGAGCGACGCGGTGATCCTGTTCGAAGCCGGCACGGAAAAGCTGGTTTCCGACATCGAGGTGATCCAGGGCATAGACA
ACCCGCGCCTGGACGAACTGTTCCCGCTGGAAATGGAACAGCTGCGCATGGAGATCGAGCTGGTCAAGGGCGCGTCCAAC
GAGTGGAGCCTGGAAGAATTCCTGGCCGGCGAGCTGACGCCGGTGTTCTTCGGCTCGGCCATCAACAACTTCGGCGTGCG
CGAAATCCTCAATGCGCTGATCGACTGGGCGCCGGCGCCGCAGGAACGCGACGCCACCGTGCGCAACGTGGACCCGAAGG
AAGCCAAGTTCTCGGGCTTCGTGTTCAAGATCCAGGCCAATATGGACCCCAAGCACCGCGACCGCATCGCCTTCCTGCGC
GTGTGCTCCGGCCAGTTCGAGCGCGGCATGAAGATGAAGCACCTGCGCCTGAACCGCGACATCGCCGCCTCCAGCGTGGT
GACCTTCATGTCCCACGACCGCGAGATCGTGGAGGAGGCTTTCGCCGGCGACATCATCGGCATCCCCAACCACGGCAACA
TCCAGATCGGCGACAGCTTCTCCGAGGGCGAAGACCTGGCCTTCACCGGCATTCCCTTCTTCGCGCCGGAACTGTTCCGC
TCGGTGCGCATCAAAAACCCGCTGAAGCTGAAGCAGCTGCAGAAAGGCCTGCAGCAGCTGGGCGAGGAAGGCGCGGTGCA
GGTATTCAAGCCGCACAGCGGCGGCGACCTGATCCTGGGCGCCGTGGGCGTGCTGCAGTTCGAAGTGGTGGCCAGCCGCC
TGGCCGCCGAATACGGCGTCGACGCCATCTTCGAATCGGCCAGCATCTGGTCCGCCCGCTGGTTCAGCTGTGACGACCGC
AAGAAGCTGGACGAATTCGTCAAGACGCTGCAGATGAACATCGCGACCGACGCCGGCGGCAACCTCGCCTATCTGGCGCC
CAACCGCGTCAACCTGCAATTGACCCAGGAGCGCTGGCCGGACATCGTTTTCCACGAAACCCGCGAACACGCGGTGAAGC
TCAACGACTGA

Upstream 100 bases:

>100_bases
CCGCATCACTCGCCATCCCGCGCGAAAGCAATATGGAATTTCCCGAAACCGGCAACTCCGGTAAAATGATCGGCTTTATT
GAATTTCAAGCAAGCCATCC

Downstream 100 bases:

>100_bases
GGAGTCCATGCACACCCTGCTCGATCTGATCGCCTTCCTGCGGGAGAACTACGCGCACGAAATCACCCGCTCCCTGCTGC
TGATCCTGACGCTGGTGGTG

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3

Number of amino acids: Translated: 536; Mature: 535

Protein sequence:

>536_residues
MSADLARIAEEVSHRRTFAIISHPDAGKTTLTEKLLLFSGAIQMAGTVKGKKGGKFATSDWMEIEKQRGISVASSVMQFD
YRDHTVNLLDTPGHQDFSEDTYRVLTAVDSALMVIDAAKGVEEQTIKLLNVCRLRNTPIVTFMNKCDREVRDSLELLDEV
ENVLKIRCAPITWPIGMGKTFRGVYSLLSDAVILFEAGTEKLVSDIEVIQGIDNPRLDELFPLEMEQLRMEIELVKGASN
EWSLEEFLAGELTPVFFGSAINNFGVREILNALIDWAPAPQERDATVRNVDPKEAKFSGFVFKIQANMDPKHRDRIAFLR
VCSGQFERGMKMKHLRLNRDIAASSVVTFMSHDREIVEEAFAGDIIGIPNHGNIQIGDSFSEGEDLAFTGIPFFAPELFR
SVRIKNPLKLKQLQKGLQQLGEEGAVQVFKPHSGGDLILGAVGVLQFEVVASRLAAEYGVDAIFESASIWSARWFSCDDR
KKLDEFVKTLQMNIATDAGGNLAYLAPNRVNLQLTQERWPDIVFHETREHAVKLND

Sequences:

>Translated_536_residues
MSADLARIAEEVSHRRTFAIISHPDAGKTTLTEKLLLFSGAIQMAGTVKGKKGGKFATSDWMEIEKQRGISVASSVMQFD
YRDHTVNLLDTPGHQDFSEDTYRVLTAVDSALMVIDAAKGVEEQTIKLLNVCRLRNTPIVTFMNKCDREVRDSLELLDEV
ENVLKIRCAPITWPIGMGKTFRGVYSLLSDAVILFEAGTEKLVSDIEVIQGIDNPRLDELFPLEMEQLRMEIELVKGASN
EWSLEEFLAGELTPVFFGSAINNFGVREILNALIDWAPAPQERDATVRNVDPKEAKFSGFVFKIQANMDPKHRDRIAFLR
VCSGQFERGMKMKHLRLNRDIAASSVVTFMSHDREIVEEAFAGDIIGIPNHGNIQIGDSFSEGEDLAFTGIPFFAPELFR
SVRIKNPLKLKQLQKGLQQLGEEGAVQVFKPHSGGDLILGAVGVLQFEVVASRLAAEYGVDAIFESASIWSARWFSCDDR
KKLDEFVKTLQMNIATDAGGNLAYLAPNRVNLQLTQERWPDIVFHETREHAVKLND
>Mature_535_residues
SADLARIAEEVSHRRTFAIISHPDAGKTTLTEKLLLFSGAIQMAGTVKGKKGGKFATSDWMEIEKQRGISVASSVMQFDY
RDHTVNLLDTPGHQDFSEDTYRVLTAVDSALMVIDAAKGVEEQTIKLLNVCRLRNTPIVTFMNKCDREVRDSLELLDEVE
NVLKIRCAPITWPIGMGKTFRGVYSLLSDAVILFEAGTEKLVSDIEVIQGIDNPRLDELFPLEMEQLRMEIELVKGASNE
WSLEEFLAGELTPVFFGSAINNFGVREILNALIDWAPAPQERDATVRNVDPKEAKFSGFVFKIQANMDPKHRDRIAFLRV
CSGQFERGMKMKHLRLNRDIAASSVVTFMSHDREIVEEAFAGDIIGIPNHGNIQIGDSFSEGEDLAFTGIPFFAPELFRS
VRIKNPLKLKQLQKGLQQLGEEGAVQVFKPHSGGDLILGAVGVLQFEVVASRLAAEYGVDAIFESASIWSARWFSCDDRK
KLDEFVKTLQMNIATDAGGNLAYLAPNRVNLQLTQERWPDIVFHETREHAVKLND

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily

Homologues:

Organism=Homo sapiens, GI18390331, Length=485, Percent_Identity=28.0412371134021, Blast_Score=180, Evalue=3e-45,
Organism=Homo sapiens, GI19923640, Length=508, Percent_Identity=24.8031496062992, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI25306283, Length=356, Percent_Identity=27.8089887640449, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI25306287, Length=483, Percent_Identity=24.6376811594203, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI157426893, Length=180, Percent_Identity=30.5555555555556, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI94966754, Length=148, Percent_Identity=31.7567567567568, Blast_Score=81, Evalue=3e-15,
Organism=Homo sapiens, GI310132016, Length=113, Percent_Identity=34.5132743362832, Blast_Score=71, Evalue=3e-12,
Organism=Homo sapiens, GI310110807, Length=113, Percent_Identity=34.5132743362832, Blast_Score=71, Evalue=3e-12,
Organism=Homo sapiens, GI310123363, Length=113, Percent_Identity=34.5132743362832, Blast_Score=71, Evalue=3e-12,
Organism=Homo sapiens, GI4503483, Length=157, Percent_Identity=29.9363057324841, Blast_Score=71, Evalue=3e-12,
Organism=Escherichia coli, GI1790835, Length=527, Percent_Identity=61.8595825426945, Blast_Score=696, Evalue=0.0,
Organism=Escherichia coli, GI1789738, Length=504, Percent_Identity=27.1825396825397, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI1788922, Length=168, Percent_Identity=32.1428571428571, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI48994988, Length=133, Percent_Identity=36.0902255639098, Blast_Score=86, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI17533571, Length=475, Percent_Identity=28.4210526315789, Blast_Score=174, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI17556745, Length=182, Percent_Identity=35.7142857142857, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17557151, Length=132, Percent_Identity=34.8484848484849, Blast_Score=89, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17506493, Length=161, Percent_Identity=31.055900621118, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71988819, Length=135, Percent_Identity=30.3703703703704, Blast_Score=71, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI71988811, Length=135, Percent_Identity=30.3703703703704, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6323098, Length=488, Percent_Identity=28.0737704918033, Blast_Score=181, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6322359, Length=524, Percent_Identity=25.1908396946565, Blast_Score=119, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6323320, Length=185, Percent_Identity=28.1081081081081, Blast_Score=79, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6324707, Length=157, Percent_Identity=30.5732484076433, Blast_Score=77, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6320593, Length=157, Percent_Identity=30.5732484076433, Blast_Score=77, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6324166, Length=161, Percent_Identity=30.4347826086957, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24582462, Length=489, Percent_Identity=29.038854805726, Blast_Score=179, Evalue=4e-45,
Organism=Drosophila melanogaster, GI221458488, Length=505, Percent_Identity=25.7425742574257, Blast_Score=145, Evalue=5e-35,
Organism=Drosophila melanogaster, GI78706572, Length=136, Percent_Identity=36.0294117647059, Blast_Score=99, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24585711, Length=567, Percent_Identity=22.3985890652557, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24585713, Length=567, Percent_Identity=22.3985890652557, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24585709, Length=567, Percent_Identity=22.3985890652557, Blast_Score=81, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RF3_CHRVO (Q7NVF7)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_902056.1
- ProteinModelPortal:   Q7NVF7
- SMR:   Q7NVF7
- GeneID:   2547741
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_2386
- NMPDR:   fig|243365.1.peg.2386
- HOGENOM:   HBG285678
- OMA:   LQFEVVQ
- PhylomeDB:   Q7NVF7
- ProtClustDB:   PRK00741
- BioCyc:   CVIO243365:CV_2386-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00072
- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000
- PRINTS:   PR00315
- TIGRFAMs:   TIGR00503
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; SSF54980 EFG_III_V; SSF50447 Translat_factor

EC number: NA

Molecular weight: Translated: 59906; Mature: 59775

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSADLARIAEEVSHRRTFAIISHPDAGKTTLTEKLLLFSGAIQMAGTVKGKKGGKFATSD
CCCHHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHEEECCCCCCCCCCHH
WMEIEKQRGISVASSVMQFDYRDHTVNLLDTPGHQDFSEDTYRVLTAVDSALMVIDAAKG
HHHHHHHCCHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHEEEHHCC
VEEQTIKLLNVCRLRNTPIVTFMNKCDREVRDSLELLDEVENVLKIRCAPITWPIGMGKT
CHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCEEECCCCCHH
FRGVYSLLSDAVILFEAGTEKLVSDIEVIQGIDNPRLDELFPLEMEQLRMEIELVKGASN
HHHHHHHHHCEEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCC
EWSLEEFLAGELTPVFFGSAINNFGVREILNALIDWAPAPQERDATVRNVDPKEAKFSGF
CCCHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCE
VFKIQANMDPKHRDRIAFLRVCSGQFERGMKMKHLRLNRDIAASSVVTFMSHDREIVEEA
EEEEECCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHHCCHHHHHHH
FAGDIIGIPNHGNIQIGDSFSEGEDLAFTGIPFFAPELFRSVRIKNPLKLKQLQKGLQQL
HCCCEEECCCCCCEEECCCCCCCCCEEEECCCHHHHHHHHHHCCCCCHHHHHHHHHHHHH
GEEGAVQVFKPHSGGDLILGAVGVLQFEVVASRLAAEYGVDAIFESASIWSARWFSCDDR
CCCCCEEEECCCCCCCEEEHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCCCEECCCCCCH
KKLDEFVKTLQMNIATDAGGNLAYLAPNRVNLQLTQERWPDIVFHETREHAVKLND
HHHHHHHHHHHHHEEECCCCCEEEECCCCEEEEECHHHCCCEEEECCCCCCEECCC
>Mature Secondary Structure 
SADLARIAEEVSHRRTFAIISHPDAGKTTLTEKLLLFSGAIQMAGTVKGKKGGKFATSD
CCHHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHEEECCCCCCCCCCHH
WMEIEKQRGISVASSVMQFDYRDHTVNLLDTPGHQDFSEDTYRVLTAVDSALMVIDAAKG
HHHHHHHCCHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHEEEHHCC
VEEQTIKLLNVCRLRNTPIVTFMNKCDREVRDSLELLDEVENVLKIRCAPITWPIGMGKT
CHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCEEECCCCCHH
FRGVYSLLSDAVILFEAGTEKLVSDIEVIQGIDNPRLDELFPLEMEQLRMEIELVKGASN
HHHHHHHHHCEEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCC
EWSLEEFLAGELTPVFFGSAINNFGVREILNALIDWAPAPQERDATVRNVDPKEAKFSGF
CCCHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCE
VFKIQANMDPKHRDRIAFLRVCSGQFERGMKMKHLRLNRDIAASSVVTFMSHDREIVEEA
EEEEECCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHHCCHHHHHHH
FAGDIIGIPNHGNIQIGDSFSEGEDLAFTGIPFFAPELFRSVRIKNPLKLKQLQKGLQQL
HCCCEEECCCCCCEEECCCCCCCCCEEEECCCHHHHHHHHHHCCCCCHHHHHHHHHHHHH
GEEGAVQVFKPHSGGDLILGAVGVLQFEVVASRLAAEYGVDAIFESASIWSARWFSCDDR
CCCCCEEEECCCCCCCEEEHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCCCEECCCCCCH
KKLDEFVKTLQMNIATDAGGNLAYLAPNRVNLQLTQERWPDIVFHETREHAVKLND
HHHHHHHHHHHHHEEECCCCCEEEECCCCEEEEECHHHCCCEEEECCCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14500782