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The map label for this gene is mccF [H]

Identifier: 34497759

GI number: 34497759

Start: 2493475

End: 2494548

Strand: Reverse

Name: mccF [H]

Synonym: CV_2304

Alternate gene names: 34497759

Gene position: 2494548-2493475 (Counterclockwise)

Preceding gene: 34497760

Following gene: 34497758

Centisome position: 52.5

GC content: 68.25

Gene sequence:

>1074_bases
ATGACTCCCGACATCAATCTTCCCCGCCGCCAGTTGCTGCAGCTGCTCGCTCTCGGCACCGGCGCCGGCATATTGCAAGG
ATGCAGCTCCATGTCCTCCCCCGCCCAGCCTTCCCAGGCCAGCCTGCGCCTCTACCTGATCGCATGCTCCGGCATCGTGC
CGGAAAAGCAGCGCCAGCTGGCGTTGCCGCGCCTGGCGCGCGCCGGCTTCTCGCTGGAGAACGCCGCCGCGCTGAACCGC
GCCTACCAGCGCTTCGCCGGCACCGACGCCGAACGGTTGTCCGATCTGCACGCGCTGCTGGACCAGCCGCAGTGGCCGCA
GCTGATCATGGCGGGCCGCGGCGGCTACGGCGCGATGCGCCTGCTGCCCAAGCTGGATCTGGCGCGACTGGGTGCGCGCC
TCAAGGAGTCCCGCTCGCTGCTGATAGGCTATAGCGACTTCTGCGCGATCCAACTGGCCTTGCTGGCCAAGACCGGCGCC
GGCAGCTTCGCCGGTCCGATGCTCGGCGATTTCGGCAGCGCCGCGCCGAACGCCTATGCCGTCAGCGAATTCATCGCCGG
CATCTCCACGCCGCGGCGCGGCCTGCGCATCGCCGGCCGCCAGCCGCAGGCCAGCGGCGAAGGCATTTTCTGGGGCGGCA
ACCTCAGCGTGCTCTCCAGCCTGGCCGGCACGCCCTATTTGCCGGACATCAAGGGCGGTCTGCTGTTCCTGGAGGACGTG
GGCGAGCAGCCGTACCGCATCGAACGCATGTTGCAGCAGCTGCATCTGGCCGGCGTGCTGGCCAAGCAAAAGGCCATCTT
CCTGGGCGACTTCTCGATGCAGCGGCATGTGGACGTCTACGATCCGCATTACAACTTCGACGCCGTGGTGGCCGAGCTTC
GCCGCATCAGCGGCGTGCCGGTGTTCACCGGCCTGCCCTTTGGCCACATCGCCGCCAAGACCACGATGCCGCTGGGCTTC
CCGGCCCGCTTCCAGGCGGAGGGAGACGGCGTGACGCTGCAATTCCTCGACTACCCGACGGTAGACGCCTCCGCCATCCA
GATCCCGGCGCTGATGGCCGAGCATCCGGTCTGA

Upstream 100 bases:

>100_bases
CCGCGCTGGAGGCCGCCTTTTCTTTTGGCGTATACTCCCGCTGACCGCGCCGCGCCCGCCGGCCCGCGGAACTCGAACTC
CGCCAACCCGTCCGATTTCC

Downstream 100 bases:

>100_bases
AAATCATGCCAGATGCTTGACCGGACAGTCCGGCTGGTGCAGTTTGACTGTCCTCGCGCAACCCATCGCGCCCATGATCA
GGAGAAAGCATGACCCGCAG

Product: microcin C7 self-immunity protein mccF

Products: NA

Alternate protein names: LD-carboxypeptidase A; Muramoyltetrapeptide carboxypeptidase [H]

Number of amino acids: Translated: 357; Mature: 356

Protein sequence:

>357_residues
MTPDINLPRRQLLQLLALGTGAGILQGCSSMSSPAQPSQASLRLYLIACSGIVPEKQRQLALPRLARAGFSLENAAALNR
AYQRFAGTDAERLSDLHALLDQPQWPQLIMAGRGGYGAMRLLPKLDLARLGARLKESRSLLIGYSDFCAIQLALLAKTGA
GSFAGPMLGDFGSAAPNAYAVSEFIAGISTPRRGLRIAGRQPQASGEGIFWGGNLSVLSSLAGTPYLPDIKGGLLFLEDV
GEQPYRIERMLQQLHLAGVLAKQKAIFLGDFSMQRHVDVYDPHYNFDAVVAELRRISGVPVFTGLPFGHIAAKTTMPLGF
PARFQAEGDGVTLQFLDYPTVDASAIQIPALMAEHPV

Sequences:

>Translated_357_residues
MTPDINLPRRQLLQLLALGTGAGILQGCSSMSSPAQPSQASLRLYLIACSGIVPEKQRQLALPRLARAGFSLENAAALNR
AYQRFAGTDAERLSDLHALLDQPQWPQLIMAGRGGYGAMRLLPKLDLARLGARLKESRSLLIGYSDFCAIQLALLAKTGA
GSFAGPMLGDFGSAAPNAYAVSEFIAGISTPRRGLRIAGRQPQASGEGIFWGGNLSVLSSLAGTPYLPDIKGGLLFLEDV
GEQPYRIERMLQQLHLAGVLAKQKAIFLGDFSMQRHVDVYDPHYNFDAVVAELRRISGVPVFTGLPFGHIAAKTTMPLGF
PARFQAEGDGVTLQFLDYPTVDASAIQIPALMAEHPV
>Mature_356_residues
TPDINLPRRQLLQLLALGTGAGILQGCSSMSSPAQPSQASLRLYLIACSGIVPEKQRQLALPRLARAGFSLENAAALNRA
YQRFAGTDAERLSDLHALLDQPQWPQLIMAGRGGYGAMRLLPKLDLARLGARLKESRSLLIGYSDFCAIQLALLAKTGAG
SFAGPMLGDFGSAAPNAYAVSEFIAGISTPRRGLRIAGRQPQASGEGIFWGGNLSVLSSLAGTPYLPDIKGGLLFLEDVG
EQPYRIERMLQQLHLAGVLAKQKAIFLGDFSMQRHVDVYDPHYNFDAVVAELRRISGVPVFTGLPFGHIAAKTTMPLGFP
ARFQAEGDGVTLQFLDYPTVDASAIQIPALMAEHPV

Specific function: Releases the terminal D-alanine residue from the cytoplasmic tetrapeptide recycling product L-Ala-gamma-D-Glu-meso- Dap-D-Ala. Can also cleave D-Ala from murein derivatives containing the tetrapeptide, i.e. MurNAc-tetrapeptide, UDP-MurNAc- tetrapeptide, G

COG id: COG1619

COG function: function code V; Uncharacterized proteins, homologs of microcin C7 resistance protein MccF

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S66 family [H]

Homologues:

Organism=Escherichia coli, GI1787441, Length=300, Percent_Identity=41.3333333333333, Blast_Score=218, Evalue=5e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003507 [H]

Pfam domain/function: PF02016 Peptidase_S66 [H]

EC number: NA

Molecular weight: Translated: 38310; Mature: 38179

Theoretical pI: Translated: 9.04; Mature: 9.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPDINLPRRQLLQLLALGTGAGILQGCSSMSSPAQPSQASLRLYLIACSGIVPEKQRQL
CCCCCCCCHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCEEEEEEEECCCCCHHHHHH
ALPRLARAGFSLENAAALNRAYQRFAGTDAERLSDLHALLDQPQWPQLIMAGRGGYGAMR
HHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCEEEECCCCCHHHH
LLPKLDLARLGARLKESRSLLIGYSDFCAIQLALLAKTGAGSFAGPMLGDFGSAAPNAYA
HHCCHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCHHH
VSEFIAGISTPRRGLRIAGRQPQASGEGIFWGGNLSVLSSLAGTPYLPDIKGGLLFLEDV
HHHHHHHCCCCCCCCEECCCCCCCCCCEEEECCCHHHHHHHCCCCCCCCCCCCEEEEECC
GEQPYRIERMLQQLHLAGVLAKQKAIFLGDFSMQRHVDVYDPHYNFDAVVAELRRISGVP
CCCCHHHHHHHHHHHHHHHHHHCCEEEEECCCHHCCCEECCCCCCHHHHHHHHHHHCCCC
VFTGLPFGHIAAKTTMPLGFPARFQAEGDGVTLQFLDYPTVDASAIQIPALMAEHPV
EEECCCHHHHHHHCCCCCCCCCEEEECCCCEEEEEECCCCCCCCHHHHHHHHHCCCC
>Mature Secondary Structure 
TPDINLPRRQLLQLLALGTGAGILQGCSSMSSPAQPSQASLRLYLIACSGIVPEKQRQL
CCCCCCCHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCEEEEEEEECCCCCHHHHHH
ALPRLARAGFSLENAAALNRAYQRFAGTDAERLSDLHALLDQPQWPQLIMAGRGGYGAMR
HHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCEEEECCCCCHHHH
LLPKLDLARLGARLKESRSLLIGYSDFCAIQLALLAKTGAGSFAGPMLGDFGSAAPNAYA
HHCCHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCHHH
VSEFIAGISTPRRGLRIAGRQPQASGEGIFWGGNLSVLSSLAGTPYLPDIKGGLLFLEDV
HHHHHHHCCCCCCCCEECCCCCCCCCCEEEECCCHHHHHHHCCCCCCCCCCCCEEEEECC
GEQPYRIERMLQQLHLAGVLAKQKAIFLGDFSMQRHVDVYDPHYNFDAVVAELRRISGVP
CCCCHHHHHHHHHHHHHHHHHHCCEEEEECCCHHCCCEECCCCCCHHHHHHHHHHHCCCC
VFTGLPFGHIAAKTTMPLGFPARFQAEGDGVTLQFLDYPTVDASAIQIPALMAEHPV
EEECCCHHHHHHHCCCCCCCCCEEEECCCCEEEEEECCCCCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]