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The map label for this gene is nemA2 [H]

Identifier: 34497700

GI number: 34497700

Start: 2432996

End: 2434177

Strand: Reverse

Name: nemA2 [H]

Synonym: CV_2245

Alternate gene names: 34497700

Gene position: 2434177-2432996 (Counterclockwise)

Preceding gene: 34497705

Following gene: 34497699

Centisome position: 51.23

GC content: 69.29

Gene sequence:

>1182_bases
TTGCAAAGCATCATGTCCTCACTGGAACGCCTCCCCCTTCGCGGAGGCCGCATACCGAGGTTGAACGACATGCCCGCACC
CGATCTGTTCTCGCCGCTGCAAGCCGGCGCCCTGCCACTGCCCAGCCGCATCGTGATGGCGCCGATGACCCGCTGCCGGA
CCGACCAGCCCGGCGACATCCCCAACGCCATGATGGCGCGCTACTATGCCCAGCGAGCCGGCGCCGGCCTGATCGTTTCC
GAAGCCACTCAGATCTCGCGCCAAGGCCAGGGCTACAGCTTCACCCCCGGCATCTACAGCCAGGACCAGATAGACGGCTG
GCGGCGGGTGACCGACGCCGTCCGCCAGGCGGGCGGACGCATGGTGCTGCAGTTGTGGCATGTGGGCCGGATGTCCCACC
CGATGTTCCATGACGGCGCGCCGCCGGTGGCGCCGTCCGCCATCGCGCCCGACGCCCAGGTCTGGGTGGCGGACGAAACA
GGCGCGGGCCGCATGGTGGATTGCCCGACGCCCCGGGCGCTGGAAACCGCCGAAATCGCCGCCATCGTCGGCGACTACCG
CCAAGCGGCCCGCAACGCGATGGCCGCCGGTTTCGACGGCGTGGAAATCCATGCGGCCAATGGCTACCTGATAGACCAGT
TCCTGCGCAGCACCTCCAACCGGCGCGACGATGCCTACGGCGGCAGCCTGGAAAACCGGCTGCGCTTCCTGATGGAGGTC
GCAGCCGCGGTGGCGGACGAAATCGGCGCCGCGCGCGTCGGCGTGCGGTTGGCTCCCTACATCACCGCGCGAGGAATGAA
TTGCCCGGAGATCGTTCCGGCGATACTGGAGGCCGCCGTCCGGCTGAATGGGCTGGGCATCGCCTACCTGCACCTTTCCG
AGGCGGACTGGGATGACGCCCCTCAGGTGGACGACGCCTTCCGCCACGCGCTGCGCGCGCGCTTCCGCGGCGCCATCATC
GTGGCCGGCCGTTATGACCAGCCGCGAGGCGAGCGCTTGCTCGCGGAAGGCCTGGCGGACCTGATCGCCTTCGGCCGGCC
CTTCATCGCCAACCCCGACCTACCCGAACGCCTGGCCCGCGGCTGGCCGCTGGCTGATTTCGACCCGTCCGCGCTGTTCG
GCGGCGACGGACGCGGCTACGACGACTACCCACGCTACCGGGAAATGGCAACCGCCCGATAA

Upstream 100 bases:

>100_bases
AAATCCGGCTGGACGGACAAAATGCCTGAGCTCCGAGTGACGCCATTTTCAAAAACCGCAAGCAAGTGATTCTGGCTAGA
ACTGGTTTTTCTCTTGCCGA

Downstream 100 bases:

>100_bases
CCGACTGACAAGGCAAACATCATGAACATCGCCCATTACGCCCAGACCCGCTACACCACCAAGGCCTTCGATCCCGGCTT
CCGGCTGAGCGCCGGCCAGA

Product: flavoprotein NADH-dependent oxidoreductase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 393; Mature: 393

Protein sequence:

>393_residues
MQSIMSSLERLPLRGGRIPRLNDMPAPDLFSPLQAGALPLPSRIVMAPMTRCRTDQPGDIPNAMMARYYAQRAGAGLIVS
EATQISRQGQGYSFTPGIYSQDQIDGWRRVTDAVRQAGGRMVLQLWHVGRMSHPMFHDGAPPVAPSAIAPDAQVWVADET
GAGRMVDCPTPRALETAEIAAIVGDYRQAARNAMAAGFDGVEIHAANGYLIDQFLRSTSNRRDDAYGGSLENRLRFLMEV
AAAVADEIGAARVGVRLAPYITARGMNCPEIVPAILEAAVRLNGLGIAYLHLSEADWDDAPQVDDAFRHALRARFRGAII
VAGRYDQPRGERLLAEGLADLIAFGRPFIANPDLPERLARGWPLADFDPSALFGGDGRGYDDYPRYREMATAR

Sequences:

>Translated_393_residues
MQSIMSSLERLPLRGGRIPRLNDMPAPDLFSPLQAGALPLPSRIVMAPMTRCRTDQPGDIPNAMMARYYAQRAGAGLIVS
EATQISRQGQGYSFTPGIYSQDQIDGWRRVTDAVRQAGGRMVLQLWHVGRMSHPMFHDGAPPVAPSAIAPDAQVWVADET
GAGRMVDCPTPRALETAEIAAIVGDYRQAARNAMAAGFDGVEIHAANGYLIDQFLRSTSNRRDDAYGGSLENRLRFLMEV
AAAVADEIGAARVGVRLAPYITARGMNCPEIVPAILEAAVRLNGLGIAYLHLSEADWDDAPQVDDAFRHALRARFRGAII
VAGRYDQPRGERLLAEGLADLIAFGRPFIANPDLPERLARGWPLADFDPSALFGGDGRGYDDYPRYREMATAR
>Mature_393_residues
MQSIMSSLERLPLRGGRIPRLNDMPAPDLFSPLQAGALPLPSRIVMAPMTRCRTDQPGDIPNAMMARYYAQRAGAGLIVS
EATQISRQGQGYSFTPGIYSQDQIDGWRRVTDAVRQAGGRMVLQLWHVGRMSHPMFHDGAPPVAPSAIAPDAQVWVADET
GAGRMVDCPTPRALETAEIAAIVGDYRQAARNAMAAGFDGVEIHAANGYLIDQFLRSTSNRRDDAYGGSLENRLRFLMEV
AAAVADEIGAARVGVRLAPYITARGMNCPEIVPAILEAAVRLNGLGIAYLHLSEADWDDAPQVDDAFRHALRARFRGAII
VAGRYDQPRGERLLAEGLADLIAFGRPFIANPDLPERLARGWPLADFDPSALFGGDGRGYDDYPRYREMATAR

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=363, Percent_Identity=48.7603305785124, Blast_Score=334, Evalue=5e-93,
Organism=Escherichia coli, GI1789463, Length=351, Percent_Identity=30.1994301994302, Blast_Score=125, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI17559804, Length=279, Percent_Identity=30.8243727598566, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17565138, Length=392, Percent_Identity=24.7448979591837, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17564188, Length=266, Percent_Identity=28.1954887218045, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17559802, Length=269, Percent_Identity=28.2527881040892, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI72001454, Length=274, Percent_Identity=28.8321167883212, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17540738, Length=267, Percent_Identity=26.5917602996255, Blast_Score=83, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6321973, Length=387, Percent_Identity=36.6925064599483, Blast_Score=214, Evalue=2e-56,
Organism=Saccharomyces cerevisiae, GI6325086, Length=385, Percent_Identity=37.1428571428571, Blast_Score=210, Evalue=2e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 42812; Mature: 42812

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSIMSSLERLPLRGGRIPRLNDMPAPDLFSPLQAGALPLPSRIVMAPMTRCRTDQPGDI
CHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCC
PNAMMARYYAQRAGAGLIVSEATQISRQGQGYSFTPGIYSQDQIDGWRRVTDAVRQAGGR
HHHHHHHHHHHHCCCCEEEHHHHHHHHCCCCCEECCCCCCCHHHHHHHHHHHHHHHCCCC
MVLQLWHVGRMSHPMFHDGAPPVAPSAIAPDAQVWVADETGAGRMVDCPTPRALETAEIA
EEHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCEEECCCCCCHHHHHHH
AIVGDYRQAARNAMAAGFDGVEIHAANGYLIDQFLRSTSNRRDDAYGGSLENRLRFLMEV
HHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
AAAVADEIGAARVGVRLAPYITARGMNCPEIVPAILEAAVRLNGLGIAYLHLSEADWDDA
HHHHHHHHHHHHHCEEEECHHHCCCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCC
PQVDDAFRHALRARFRGAIIVAGRYDQPRGERLLAEGLADLIAFGRPFIANPDLPERLAR
CCHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHC
GWPLADFDPSALFGGDGRGYDDYPRYREMATAR
CCCCCCCCCCCEECCCCCCCCCCHHHHHHHCCC
>Mature Secondary Structure
MQSIMSSLERLPLRGGRIPRLNDMPAPDLFSPLQAGALPLPSRIVMAPMTRCRTDQPGDI
CHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCC
PNAMMARYYAQRAGAGLIVSEATQISRQGQGYSFTPGIYSQDQIDGWRRVTDAVRQAGGR
HHHHHHHHHHHHCCCCEEEHHHHHHHHCCCCCEECCCCCCCHHHHHHHHHHHHHHHCCCC
MVLQLWHVGRMSHPMFHDGAPPVAPSAIAPDAQVWVADETGAGRMVDCPTPRALETAEIA
EEHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCEEECCCCCCHHHHHHH
AIVGDYRQAARNAMAAGFDGVEIHAANGYLIDQFLRSTSNRRDDAYGGSLENRLRFLMEV
HHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
AAAVADEIGAARVGVRLAPYITARGMNCPEIVPAILEAAVRLNGLGIAYLHLSEADWDDA
HHHHHHHHHHHHHCEEEECHHHCCCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCC
PQVDDAFRHALRARFRGAIIVAGRYDQPRGERLLAEGLADLIAFGRPFIANPDLPERLAR
CCHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHC
GWPLADFDPSALFGGDGRGYDDYPRYREMATAR
CCCCCCCCCCCEECCCCCCCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]