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The map label for this gene is nfnB [H]

Identifier: 34497699

GI number: 34497699

Start: 2432321

End: 2432974

Strand: Reverse

Name: nfnB [H]

Synonym: CV_2244

Alternate gene names: 34497699

Gene position: 2432974-2432321 (Counterclockwise)

Preceding gene: 34497700

Following gene: 34497698

Centisome position: 51.21

GC content: 66.06

Gene sequence:

>654_bases
ATGAACATCGCCCATTACGCCCAGACCCGCTACACCACCAAGGCCTTCGATCCCGGCTTCCGGCTGAGCGCCGGCCAGAT
CGAACAGATCGAAACCCTGCTGCGCCACAGCCCATCGTCCACCAACTCCCAGCCCTGGCATTTCTTCATCGCCGGCAGCG
ACGAGAGCAAAGCCCGCGTCGCCAAGGCCACCGCCGACGGCTATGCCTTCAACCAGGCCAAGGTGCTCAACGCCTCCCAC
GTGGTGGTATTCTGCACCCGCGCCGCGCTGGACGACGCCTACCTGCGAACGCTGCTGGACCAAGAAGAGCGAGACGGCCG
TTTCGCCAGCCCGGAGGCCCGCGCAGGCCAGCACAAGGGCCGCTCCCACTTCGCCGACATGCACCGCTTCGAGCTGCGCG
ACGCCCCGCACTGGATGGAAAAACAGGTTTATCTGGCGGTAGGCACCCTGCTCCTGGGCGCGGCGGCGCTGGAAATCGAC
GCTTGCCCGATAGAAGGCTTCGACCAGCGGACTCTGGGAGAGGAGCTGGGCTTGCGGGAAAAAGGGCTGATCGCTTCGGT
GATCGTCGCCCTGGGCCGCCGCTCCGACGAAGACTTCAACGCCAGGCTGCCGAAGTCCCGCCTGCCGGCCGAGGCCGTCA
TCACCCGTCTGTGA

Upstream 100 bases:

>100_bases
CCCGTCCGCGCTGTTCGGCGGCGACGGACGCGGCTACGACGACTACCCACGCTACCGGGAAATGGCAACCGCCCGATAAC
CGACTGACAAGGCAAACATC

Downstream 100 bases:

>100_bases
CGCAAAACCGCGCCCGCGCGGCGCGCAAGGAGTCCGGGATGAACATCCAGGGAATCGACCATTTCACGATACGCACTGCC
GACCTGGAGGCCAGCGCGGT

Product: dihydropteridine reductase

Products: NA

Alternate protein names: NR [H]

Number of amino acids: Translated: 217; Mature: 217

Protein sequence:

>217_residues
MNIAHYAQTRYTTKAFDPGFRLSAGQIEQIETLLRHSPSSTNSQPWHFFIAGSDESKARVAKATADGYAFNQAKVLNASH
VVVFCTRAALDDAYLRTLLDQEERDGRFASPEARAGQHKGRSHFADMHRFELRDAPHWMEKQVYLAVGTLLLGAAALEID
ACPIEGFDQRTLGEELGLREKGLIASVIVALGRRSDEDFNARLPKSRLPAEAVITRL

Sequences:

>Translated_217_residues
MNIAHYAQTRYTTKAFDPGFRLSAGQIEQIETLLRHSPSSTNSQPWHFFIAGSDESKARVAKATADGYAFNQAKVLNASH
VVVFCTRAALDDAYLRTLLDQEERDGRFASPEARAGQHKGRSHFADMHRFELRDAPHWMEKQVYLAVGTLLLGAAALEID
ACPIEGFDQRTLGEELGLREKGLIASVIVALGRRSDEDFNARLPKSRLPAEAVITRL
>Mature_217_residues
MNIAHYAQTRYTTKAFDPGFRLSAGQIEQIETLLRHSPSSTNSQPWHFFIAGSDESKARVAKATADGYAFNQAKVLNASH
VVVFCTRAALDDAYLRTLLDQEERDGRFASPEARAGQHKGRSHFADMHRFELRDAPHWMEKQVYLAVGTLLLGAAALEID
ACPIEGFDQRTLGEELGLREKGLIASVIVALGRRSDEDFNARLPKSRLPAEAVITRL

Specific function: Reduction of a variety of nitroaromatic compounds using NADH (and to lesser extent NADPH) as source of reducing equivalents; two electrons are transferred [H]

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitroreductase family [H]

Homologues:

Organism=Escherichia coli, GI1786792, Length=217, Percent_Identity=57.6036866359447, Blast_Score=265, Evalue=2e-72,

Paralogues:

None

Copy number: 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000415 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: 1.-.-.-; 1.5.1.34

Molecular weight: Translated: 24123; Mature: 24123

Theoretical pI: Translated: 7.27; Mature: 7.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIAHYAQTRYTTKAFDPGFRLSAGQIEQIETLLRHSPSSTNSQPWHFFIAGSDESKARV
CCCCHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCHHHH
AKATADGYAFNQAKVLNASHVVVFCTRAALDDAYLRTLLDQEERDGRFASPEARAGQHKG
HHHCCCCCCCCCEEEECCCEEEEEEEHHHHHHHHHHHHHCCHHCCCCCCCCCCCCCCCCC
RSHFADMHRFELRDAPHWMEKQVYLAVGTLLLGAAALEIDACPIEGFDQRTLGEELGLRE
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCHHHHHHHHCCCC
KGLIASVIVALGRRSDEDFNARLPKSRLPAEAVITRL
CCHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHCC
>Mature Secondary Structure
MNIAHYAQTRYTTKAFDPGFRLSAGQIEQIETLLRHSPSSTNSQPWHFFIAGSDESKARV
CCCCHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCHHHH
AKATADGYAFNQAKVLNASHVVVFCTRAALDDAYLRTLLDQEERDGRFASPEARAGQHKG
HHHCCCCCCCCCEEEECCCEEEEEEEHHHHHHHHHHHHHCCHHCCCCCCCCCCCCCCCCC
RSHFADMHRFELRDAPHWMEKQVYLAVGTLLLGAAALEIDACPIEGFDQRTLGEELGLRE
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCHHHHHHHHCCCC
KGLIASVIVALGRRSDEDFNARLPKSRLPAEAVITRL
CCHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1999406; 1999405 [H]