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The map label for this gene is cca

Identifier: 34497487

GI number: 34497487

Start: 2214113

End: 2215336

Strand: Reverse

Name: cca

Synonym: CV_2032

Alternate gene names: 34497487

Gene position: 2215336-2214113 (Counterclockwise)

Preceding gene: 34497488

Following gene: 34497486

Centisome position: 46.63

GC content: 69.12

Gene sequence:

>1224_bases
ATGGAAATCTACATCGTCGGCGGCGCCGTCCGCGACCGTCTGCTGGGCCTTCCGGTCAAGGACAGGGACTGGGTGGTGGT
CGGCTCTACTCCGGACGAGATGCTGGCGCAGGGCTACCGCCCCGTAGGCAAAGACTTCCCCGTGTTCCTGCACCCTGAAA
CCCAGGAGGAATACGCGCTGGCGCGCACCGAACGCAAGATTGCCAAGGGCTACCACGGCTTCACCTTCCATACTTCGCCC
GAGGTGACGCTGGAAGAAGACCTGGCCCGGCGCGACCTGACCATCAATGCGATCGCCGAAGCGGCCGACGGCAGCCTGAC
CGACCCGTATGGCGGGCAGGAAGACCTGAAGGCGGGCGTGCTGCGCCACGTCAGCGAGGCTTTCGCCGAAGACCCGGTGC
GCATCCTGCGGCTGGCCCGCTTCGCGGCCCGCTTCGATTTCGCGGTGGCGCCGGAAACCATGGCGCTGATGCGGCGGATG
GTGGACGACGGCGAGGCGGACGCGCTGGTGGCGGAGCGGGTATGGCAGGAGTTGGCCAAGGGCCTGATGGAGGACAAGCC
GTCGCGCATGTTCCTGACGCTGCGCGAATGCGGCGCGCTCGCGCGCATCCTGCCCGAGGTGGACGCGCTGTTCGGCGTGC
CGCAGCGCGCCGACCACCACCCGGAGATAGACTGCGGCGACCACGTCATGCGGGTGCTGGACTACGCCGCGGCCGCCGGC
CAGCCGCTGGCGGTGCGCTTCGCGGCGCTGGGGCACGACCTGGGCAAGGCGCTGACGCCGGCCAAGGTGCTGCCGCGCCA
CATCGGCCACGAAGAAGGGGGCATCGCGCCGCTGGCGGAACTGTGCCGCCGGCTGCGCGTGCCCAACGACTGCCGCGACC
TCGCCCACATCACCATGGTGCACCACACCAAGGTGCACCGCGCGCTGGAACTGCGCCCCGACACCGTGCTGCGCCTGCTG
AAGGACTGCGACGCGCTGCGCCGTCCCGAACGCTTCCTGCAGATGCTGGACGCCTGCCTGGCAGACACTCGCGGCAGGCT
CGGCTTCGAACGCGCGCCCTATCCGCAAAAGGACTACCTGCAAGCGCAGCTGGCTGCTACGCTGCAAATTGACGCCGGCG
CCATCGCCGCCGGCTGCGCCGACAAGGCCGCGATTCCGGCCACGATAGACGCCGCGCGCACCGCAGTCATCGCCAAGTGC
AAGGAGGAATGGAAGGAGGACTGA

Upstream 100 bases:

>100_bases
TTCGAACCCGCCGCGCTGTCGGCGCTGGCGCCCGGCTGGCTGGCCGGCCGCGACCGCAACGACACCGCGTCCCGCTACCG
CGCGAAGGCGGCGCGCTGAT

Downstream 100 bases:

>100_bases
TGGACAACGGCTATCGCCTCGCCGCCACGCTGATGCGCGGCGGAACCAGCAAGGGGCTGTTCATCCGCGCGGACGCGCTG
CCCGAAGACACCGAGCTATG

Product: tRNA adenylyltransferase

Products: NA

Alternate protein names: CCA-adding enzyme; tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl-transferase; tRNA nucleotidyltransferase; tRNA-NT; 2'-nucleotidase; 2',3'-cyclic phosphodiesterase; Phosphatase

Number of amino acids: Translated: 407; Mature: 407

Protein sequence:

>407_residues
MEIYIVGGAVRDRLLGLPVKDRDWVVVGSTPDEMLAQGYRPVGKDFPVFLHPETQEEYALARTERKIAKGYHGFTFHTSP
EVTLEEDLARRDLTINAIAEAADGSLTDPYGGQEDLKAGVLRHVSEAFAEDPVRILRLARFAARFDFAVAPETMALMRRM
VDDGEADALVAERVWQELAKGLMEDKPSRMFLTLRECGALARILPEVDALFGVPQRADHHPEIDCGDHVMRVLDYAAAAG
QPLAVRFAALGHDLGKALTPAKVLPRHIGHEEGGIAPLAELCRRLRVPNDCRDLAHITMVHHTKVHRALELRPDTVLRLL
KDCDALRRPERFLQMLDACLADTRGRLGFERAPYPQKDYLQAQLAATLQIDAGAIAAGCADKAAIPATIDAARTAVIAKC
KEEWKED

Sequences:

>Translated_407_residues
MEIYIVGGAVRDRLLGLPVKDRDWVVVGSTPDEMLAQGYRPVGKDFPVFLHPETQEEYALARTERKIAKGYHGFTFHTSP
EVTLEEDLARRDLTINAIAEAADGSLTDPYGGQEDLKAGVLRHVSEAFAEDPVRILRLARFAARFDFAVAPETMALMRRM
VDDGEADALVAERVWQELAKGLMEDKPSRMFLTLRECGALARILPEVDALFGVPQRADHHPEIDCGDHVMRVLDYAAAAG
QPLAVRFAALGHDLGKALTPAKVLPRHIGHEEGGIAPLAELCRRLRVPNDCRDLAHITMVHHTKVHRALELRPDTVLRLL
KDCDALRRPERFLQMLDACLADTRGRLGFERAPYPQKDYLQAQLAATLQIDAGAIAAGCADKAAIPATIDAARTAVIAKC
KEEWKED
>Mature_407_residues
MEIYIVGGAVRDRLLGLPVKDRDWVVVGSTPDEMLAQGYRPVGKDFPVFLHPETQEEYALARTERKIAKGYHGFTFHTSP
EVTLEEDLARRDLTINAIAEAADGSLTDPYGGQEDLKAGVLRHVSEAFAEDPVRILRLARFAARFDFAVAPETMALMRRM
VDDGEADALVAERVWQELAKGLMEDKPSRMFLTLRECGALARILPEVDALFGVPQRADHHPEIDCGDHVMRVLDYAAAAG
QPLAVRFAALGHDLGKALTPAKVLPRHIGHEEGGIAPLAELCRRLRVPNDCRDLAHITMVHHTKVHRALELRPDTVLRLL
KDCDALRRPERFLQMLDACLADTRGRLGFERAPYPQKDYLQAQLAATLQIDAGAIAAGCADKAAIPATIDAARTAVIAKC
KEEWKED

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1789436, Length=406, Percent_Identity=51.9704433497537, Blast_Score=411, Evalue=1e-116,
Organism=Escherichia coli, GI87081691, Length=168, Percent_Identity=36.9047619047619, Blast_Score=85, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6321016, Length=217, Percent_Identity=32.258064516129, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI281360112, Length=317, Percent_Identity=26.4984227129338, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI21357337, Length=317, Percent_Identity=26.4984227129338, Blast_Score=78, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CCA_CHRVO (Q7MBF4)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_901702.1
- ProteinModelPortal:   Q7MBF4
- SMR:   Q7MBF4
- GeneID:   2548344
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_2032
- NMPDR:   fig|243365.1.peg.2032
- HOGENOM:   HBG635063
- OMA:   RWACLLH
- PhylomeDB:   Q7MBF4
- ProtClustDB:   CLSK2485444
- BioCyc:   CVIO243365:CV_2032-MONOMER
- BRENDA:   2.7.7.21
- BRENDA:   2.7.7.25
- HAMAP:   MF_01261
- InterPro:   IPR012006
- InterPro:   IPR003607
- InterPro:   IPR002646
- PIRSF:   PIRSF000813
- SMART:   SM00471

Pfam domain/function: PF01743 PolyA_pol

EC number: =2.7.7.21; =2.7.7.25

Molecular weight: Translated: 45022; Mature: 45022

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: NA

Important sites: BINDING 8-8 BINDING 11-11 BINDING 91-91 BINDING 137-137 BINDING 140-140

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIYIVGGAVRDRLLGLPVKDRDWVVVGSTPDEMLAQGYRPVGKDFPVFLHPETQEEYAL
CEEEEECHHHHHHHHCCCCCCCCEEEECCCHHHHHHCCCCCCCCCCCEEECCCCHHHHHH
ARTERKIAKGYHGFTFHTSPEVTLEEDLARRDLTINAIAEAADGSLTDPYGGQEDLKAGV
HHHHHHHHCCCCCCEEECCCCCCHHHHHHHHCCHHHHHHHHCCCCCCCCCCCHHHHHHHH
LRHVSEAFAEDPVRILRLARFAARFDFAVAPETMALMRRMVDDGEADALVAERVWQELAK
HHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
GLMEDKPSRMFLTLRECGALARILPEVDALFGVPQRADHHPEIDCGDHVMRVLDYAAAAG
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCC
QPLAVRFAALGHDLGKALTPAKVLPRHIGHEEGGIAPLAELCRRLRVPNDCRDLAHITMV
CCHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHH
HHTKVHRALELRPDTVLRLLKDCDALRRPERFLQMLDACLADTRGRLGFERAPYPQKDYL
HHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
QAQLAATLQIDAGAIAAGCADKAAIPATIDAARTAVIAKCKEEWKED
HHHHHHHEEECCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEIYIVGGAVRDRLLGLPVKDRDWVVVGSTPDEMLAQGYRPVGKDFPVFLHPETQEEYAL
CEEEEECHHHHHHHHCCCCCCCCEEEECCCHHHHHHCCCCCCCCCCCEEECCCCHHHHHH
ARTERKIAKGYHGFTFHTSPEVTLEEDLARRDLTINAIAEAADGSLTDPYGGQEDLKAGV
HHHHHHHHCCCCCCEEECCCCCCHHHHHHHHCCHHHHHHHHCCCCCCCCCCCHHHHHHHH
LRHVSEAFAEDPVRILRLARFAARFDFAVAPETMALMRRMVDDGEADALVAERVWQELAK
HHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
GLMEDKPSRMFLTLRECGALARILPEVDALFGVPQRADHHPEIDCGDHVMRVLDYAAAAG
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCC
QPLAVRFAALGHDLGKALTPAKVLPRHIGHEEGGIAPLAELCRRLRVPNDCRDLAHITMV
CCHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHH
HHTKVHRALELRPDTVLRLLKDCDALRRPERFLQMLDACLADTRGRLGFERAPYPQKDYL
HHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
QAQLAATLQIDAGAIAAGCADKAAIPATIDAARTAVIAKCKEEWKED
HHHHHHHEEECCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14500782