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The map label for this gene is phnM [H]

Identifier: 34497295

GI number: 34497295

Start: 1995415

End: 1996551

Strand: Reverse

Name: phnM [H]

Synonym: CV_1840

Alternate gene names: 34497295

Gene position: 1996551-1995415 (Counterclockwise)

Preceding gene: 34497296

Following gene: 34497294

Centisome position: 42.02

GC content: 66.93

Gene sequence:

>1137_bases
ATGAAACAGATTTTGAACCACGCCCGCATCGTGCTGGCCGACCGCGTCATCGACAACGGCGCGCTGGAAATCAACGACGG
CCGGATCGCCGCGATACACGAACACGCCCTGCCCGATGGAGAGGATTTGGCCGGCGCCCTGCTGCTGCCCGGCTTGGTGG
AGATCCACACCGACAATCTGGAAAAACACCTGATGCCGCGCAACGGCGTGCTGTGGCCGATTTTGCCGGCGGTGATCACC
CACGACGCCCAGTGCGTGGCCGCCGGCATCACCACGGTGCTGGACGCGCTGGCGGTGGGCGATCTGGAGGGCGAAAGCGT
GCGGCTGGACACACTGCACAGTTCGTTCGACGCCATCAACCAGGGAATGCGCGAAGGCGCGCTGCGCGCCGACCACTTTT
TCCATCTGCGCTGCGAGCTGGCCTATCCGGGATTGCTGGACGAGCTGGAAGCGCTGATCGCCCACCCGGCGGTGAAGCTG
GTATCGGTGATGGACCACACGCCGGGCCAGCGCCAGTACCGCGACCTGGAGCAGTACAAGAAGTACTACGGCAAGAAGAA
AGTGGGCTGGAACGAAGACACCTTTCTCGCCGCGGTGGCCGAACGCAAAACCAAGCAGGCGCAATACGCTGGCCGCCACA
AACAGGCGGTAGTGGAGCTGGCGCAGGCGCGCGGCATCGCGCTGGCCAGCCATGACGACACCGACGAGGCGCATATCGAG
GAGGCGCTGCGCGACGGCGTGGCGATCTCGGAATTCCCCACCACGCGGGAAGCCGCCGCAGCAGCGAAACGACACGGCCT
CGCCACCGTGATGGGCGCACCCAATGTGGTGCGCGGCGGCTCCCACTCCGGCAATGTGGCGGCGCTGGAGCTGGCTCGCC
ATGACTTGCTGGACATCCTGTCGTCCGACTACGTGCCCGCCAGCCTGCTGCACGGCGCCTGGCTGCTGCACGCGCAGGCG
GGCTGGAGCCTGCCGCGCGCCGTCGCCGCCGTCAGCCTGAACCCGGCGCACTCGGTGGGCCTGGCCGATCGCGGCGACAT
CGCCGTCGGCCAGCGCGCCGATCTGGTGGCCATGATCGTTCATGGCGACGTGCCGCTGGTGCGGCAAGTCTGGAACAGCG
GCAGGAGGGTATTCTGA

Upstream 100 bases:

>100_bases
GCGCGGCGCTGATCGGCATCTTCCACGACGCGCCCACCCGCGACGCCATCGCCACCCGCGTGTTCCAGGTTCCCCTGGCG
CGACAAACGGAAACCGCAGC

Downstream 100 bases:

>100_bases
TGCGACAAGCGATCGCCACGTTGCAACAACTGTACCGCGAGCACGGCGCGCGCCATTACGGCGAGGCCGTCAGCCAGTTC
GACCACGCGATCCAGGCCGC

Product: phosphonate metabolism protein PhnM

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MKQILNHARIVLADRVIDNGALEINDGRIAAIHEHALPDGEDLAGALLLPGLVEIHTDNLEKHLMPRNGVLWPILPAVIT
HDAQCVAAGITTVLDALAVGDLEGESVRLDTLHSSFDAINQGMREGALRADHFFHLRCELAYPGLLDELEALIAHPAVKL
VSVMDHTPGQRQYRDLEQYKKYYGKKKVGWNEDTFLAAVAERKTKQAQYAGRHKQAVVELAQARGIALASHDDTDEAHIE
EALRDGVAISEFPTTREAAAAAKRHGLATVMGAPNVVRGGSHSGNVAALELARHDLLDILSSDYVPASLLHGAWLLHAQA
GWSLPRAVAAVSLNPAHSVGLADRGDIAVGQRADLVAMIVHGDVPLVRQVWNSGRRVF

Sequences:

>Translated_378_residues
MKQILNHARIVLADRVIDNGALEINDGRIAAIHEHALPDGEDLAGALLLPGLVEIHTDNLEKHLMPRNGVLWPILPAVIT
HDAQCVAAGITTVLDALAVGDLEGESVRLDTLHSSFDAINQGMREGALRADHFFHLRCELAYPGLLDELEALIAHPAVKL
VSVMDHTPGQRQYRDLEQYKKYYGKKKVGWNEDTFLAAVAERKTKQAQYAGRHKQAVVELAQARGIALASHDDTDEAHIE
EALRDGVAISEFPTTREAAAAAKRHGLATVMGAPNVVRGGSHSGNVAALELARHDLLDILSSDYVPASLLHGAWLLHAQA
GWSLPRAVAAVSLNPAHSVGLADRGDIAVGQRADLVAMIVHGDVPLVRQVWNSGRRVF
>Mature_378_residues
MKQILNHARIVLADRVIDNGALEINDGRIAAIHEHALPDGEDLAGALLLPGLVEIHTDNLEKHLMPRNGVLWPILPAVIT
HDAQCVAAGITTVLDALAVGDLEGESVRLDTLHSSFDAINQGMREGALRADHFFHLRCELAYPGLLDELEALIAHPAVKL
VSVMDHTPGQRQYRDLEQYKKYYGKKKVGWNEDTFLAAVAERKTKQAQYAGRHKQAVVELAQARGIALASHDDTDEAHIE
EALRDGVAISEFPTTREAAAAAKRHGLATVMGAPNVVRGGSHSGNVAALELARHDLLDILSSDYVPASLLHGAWLLHAQA
GWSLPRAVAAVSLNPAHSVGLADRGDIAVGQRADLVAMIVHGDVPLVRQVWNSGRRVF

Specific function: Belongs to an operon involved in alkylphosphonate uptake and C-P lyase. Exact function not known [H]

COG id: COG3454

COG function: function code P; Metal-dependent hydrolase involved in phosphonate metabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790533, Length=379, Percent_Identity=45.910290237467, Blast_Score=315, Evalue=3e-87,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013108
- InterPro:   IPR011059
- InterPro:   IPR012696 [H]

Pfam domain/function: PF07969 Amidohydro_3 [H]

EC number: NA

Molecular weight: Translated: 40860; Mature: 40860

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQILNHARIVLADRVIDNGALEINDGRIAAIHEHALPDGEDLAGALLLPGLVEIHTDNL
CHHHHHHHHHHHHHHHCCCCCEEECCCEEEEEEHHCCCCCCHHHHHHHCCCCEEEECCCH
EKHLMPRNGVLWPILPAVITHDAQCVAAGITTVLDALAVGDLEGESVRLDTLHSSFDAIN
HHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHH
QGMREGALRADHFFHLRCELAYPGLLDELEALIAHPAVKLVSVMDHTPGQRQYRDLEQYK
HHHHHCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
KYYGKKKVGWNEDTFLAAVAERKTKQAQYAGRHKQAVVELAQARGIALASHDDTDEAHIE
HHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCHHHHH
EALRDGVAISEFPTTREAAAAAKRHGLATVMGAPNVVRGGSHSGNVAALELARHDLLDIL
HHHHCCCEECCCCCHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCEEHHHHHHHHHHHHH
SSDYVPASLLHGAWLLHAQAGWSLPRAVAAVSLNPAHSVGLADRGDIAVGQRADLVAMIV
HCCCCCHHHHHHHHEEEECCCCCCHHHHHEEECCCHHCCCCCCCCCCCCCCCCCEEEEEE
HGDVPLVRQVWNSGRRVF
ECCCHHHHHHHHCCCCCC
>Mature Secondary Structure
MKQILNHARIVLADRVIDNGALEINDGRIAAIHEHALPDGEDLAGALLLPGLVEIHTDNL
CHHHHHHHHHHHHHHHCCCCCEEECCCEEEEEEHHCCCCCCHHHHHHHCCCCEEEECCCH
EKHLMPRNGVLWPILPAVITHDAQCVAAGITTVLDALAVGDLEGESVRLDTLHSSFDAIN
HHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHH
QGMREGALRADHFFHLRCELAYPGLLDELEALIAHPAVKLVSVMDHTPGQRQYRDLEQYK
HHHHHCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
KYYGKKKVGWNEDTFLAAVAERKTKQAQYAGRHKQAVVELAQARGIALASHDDTDEAHIE
HHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCHHHHH
EALRDGVAISEFPTTREAAAAAKRHGLATVMGAPNVVRGGSHSGNVAALELARHDLLDIL
HHHHCCCEECCCCCHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCEEHHHHHHHHHHHHH
SSDYVPASLLHGAWLLHAQAGWSLPRAVAAVSLNPAHSVGLADRGDIAVGQRADLVAMIV
HCCCCCHHHHHHHHEEEECCCCCCHHHHHEEECCCHHCCCCCCCCCCCCCCCCCEEEEEE
HGDVPLVRQVWNSGRRVF
ECCCHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2155230; 1840580; 7610040; 9278503 [H]