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The map label for this gene is cysW [H]

Identifier: 34497284

GI number: 34497284

Start: 1987709

End: 1988587

Strand: Reverse

Name: cysW [H]

Synonym: CV_1829

Alternate gene names: 34497284

Gene position: 1988587-1987709 (Counterclockwise)

Preceding gene: 34497285

Following gene: 34497283

Centisome position: 41.86

GC content: 68.15

Gene sequence:

>879_bases
ATGAGCGCCGCCATCACCCATCCGCGCCCGGCGACAGACAAGGCCGCGCAACTGGAATCCCGCGCCGCCACCCGCGAGTC
GCGCGCCGTCAAGTGGACCATCCTGGCGCTGTCGCTGGGCTTTTTCGCCGTGTTCCTGCTGCTGCCGCTGTTCGTCGTGT
TCGCCGAGGCGCTGGCCAAGGGCTGGAGCACCTATCTGACCGCGCTGTCCGAGCCCGACGCGCTGGCGGCGGTGAGGCTG
ACGCTGCTGGCGGCGGCGGTGTCGGTGCCGCTGAACCTGGCCTTCGGCATCGCCGCCGCCTGGGCGGTCACCCGTTTCGA
CTTCCGCGGCAAGCAGCTGCTGATCACGCTGATCGACCTGCCGTTCTCGGTGTCGCCGGTGGTCGCCGGCCTGATCTTCG
TGCTGGTGTTCGGCCAGCAAGGCTGGCTGGGGCCGCGGCTGGCTGAGTACGGCATCAAGATCATTTTCTCGGTGCCCGGC
ATCGTGCTGGCCACCTTGTTCATCACCGTGCCCTTCGTCGCCCGCGAGCTGATCCCGCTGATGGAGGCCCAGGGCCGCGA
GGAGGAGGAAGCCGCGGTGGTGCTGGGCGCGCGCGGCTGGCAGGTGCTGTGGCACGTGACCCTGCCGAATGTGCGCTGGG
CGCTGCTGTACGGCGTGATCCTCAGCAACGCGCGGGCGATGGGCGAGTTCGGCGCGGTGTCGGTGGTGTCCGGCCACATC
CGCGGCGAAACCAATACCCTGCCTTTGCACGTGGAAATCCTCTACAACGAGTACAATTTCGCCGCGGCCTTCGCCGTGGC
GTCCCTGCTGGCCTTGCTGGCCCTGGTCACGCTGGCGCTGAAGAGCTGGGTGGAATGGCGCAGCGTCAAGGAAAACTGA

Upstream 100 bases:

>100_bases
TCGCCAGCGTGATGCTGGCCGCGTCCTTCCTGCTGTTGCTGGCGATCAACGGCCTGCAGTTCTGGAGCGCGCGCCGACAC
GGCCGCGCGGGAGGCGCCCG

Downstream 100 bases:

>100_bases
GATGAGCATACAAGTAGAAAACATCCGCAAGGCCTTCGGCGACTTCGTCGCGCTGAACGACATCAGCCTCAACTTCCCCG
GCGGCGAGCTGGTGGCGCTG

Product: sulfate transport system permease CysW

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 292; Mature: 291

Protein sequence:

>292_residues
MSAAITHPRPATDKAAQLESRAATRESRAVKWTILALSLGFFAVFLLLPLFVVFAEALAKGWSTYLTALSEPDALAAVRL
TLLAAAVSVPLNLAFGIAAAWAVTRFDFRGKQLLITLIDLPFSVSPVVAGLIFVLVFGQQGWLGPRLAEYGIKIIFSVPG
IVLATLFITVPFVARELIPLMEAQGREEEEAAVVLGARGWQVLWHVTLPNVRWALLYGVILSNARAMGEFGAVSVVSGHI
RGETNTLPLHVEILYNEYNFAAAFAVASLLALLALVTLALKSWVEWRSVKEN

Sequences:

>Translated_292_residues
MSAAITHPRPATDKAAQLESRAATRESRAVKWTILALSLGFFAVFLLLPLFVVFAEALAKGWSTYLTALSEPDALAAVRL
TLLAAAVSVPLNLAFGIAAAWAVTRFDFRGKQLLITLIDLPFSVSPVVAGLIFVLVFGQQGWLGPRLAEYGIKIIFSVPG
IVLATLFITVPFVARELIPLMEAQGREEEEAAVVLGARGWQVLWHVTLPNVRWALLYGVILSNARAMGEFGAVSVVSGHI
RGETNTLPLHVEILYNEYNFAAAFAVASLLALLALVTLALKSWVEWRSVKEN
>Mature_291_residues
SAAITHPRPATDKAAQLESRAATRESRAVKWTILALSLGFFAVFLLLPLFVVFAEALAKGWSTYLTALSEPDALAAVRLT
LLAAAVSVPLNLAFGIAAAWAVTRFDFRGKQLLITLIDLPFSVSPVVAGLIFVLVFGQQGWLGPRLAEYGIKIIFSVPGI
VLATLFITVPFVARELIPLMEAQGREEEEAAVVLGARGWQVLWHVTLPNVRWALLYGVILSNARAMGEFGAVSVVSGHIR
GETNTLPLHVEILYNEYNFAAAFAVASLLALLALVTLALKSWVEWRSVKEN

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4208

COG function: function code P; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI87082099, Length=268, Percent_Identity=53.3582089552239, Blast_Score=293, Evalue=6e-81,
Organism=Escherichia coli, GI1788764, Length=209, Percent_Identity=40.1913875598086, Blast_Score=152, Evalue=3e-38,
Organism=Escherichia coli, GI1786980, Length=218, Percent_Identity=33.4862385321101, Blast_Score=96, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011866
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 31678; Mature: 31546

Theoretical pI: Translated: 8.87; Mature: 8.87

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAAITHPRPATDKAAQLESRAATRESRAVKWTILALSLGFFAVFLLLPLFVVFAEALAK
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GWSTYLTALSEPDALAAVRLTLLAAAVSVPLNLAFGIAAAWAVTRFDFRGKQLLITLIDL
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECC
PFSVSPVVAGLIFVLVFGQQGWLGPRLAEYGIKIIFSVPGIVLATLFITVPFVARELIPL
CCCHHHHHHHHHHHHHHCCCCCCCCHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHH
MEAQGREEEEAAVVLGARGWQVLWHVTLPNVRWALLYGVILSNARAMGEFGAVSVVSGHI
HHHCCCCCCCCEEEEECCCCEEEEEEECCCHHHHHHHHHHHHCCHHHCCCCCHHEEEEEE
RGETNTLPLHVEILYNEYNFAAAFAVASLLALLALVTLALKSWVEWRSVKEN
CCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SAAITHPRPATDKAAQLESRAATRESRAVKWTILALSLGFFAVFLLLPLFVVFAEALAK
CCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GWSTYLTALSEPDALAAVRLTLLAAAVSVPLNLAFGIAAAWAVTRFDFRGKQLLITLIDL
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECC
PFSVSPVVAGLIFVLVFGQQGWLGPRLAEYGIKIIFSVPGIVLATLFITVPFVARELIPL
CCCHHHHHHHHHHHHHHCCCCCCCCHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHH
MEAQGREEEEAAVVLGARGWQVLWHVTLPNVRWALLYGVILSNARAMGEFGAVSVVSGHI
HHHCCCCCCCCEEEEECCCCEEEEEEECCCHHHHHHHHHHHHCCHHHCCCCCHHEEEEEE
RGETNTLPLHVEILYNEYNFAAAFAVASLLALLALVTLALKSWVEWRSVKEN
CCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]