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The map label for this gene is pycA [H]

Identifier: 34497262

GI number: 34497262

Start: 1965214

End: 1966704

Strand: Direct

Name: pycA [H]

Synonym: CV_1807

Alternate gene names: 34497262

Gene position: 1965214-1966704 (Clockwise)

Preceding gene: 34497261

Following gene: 34497263

Centisome position: 41.36

GC content: 68.68

Gene sequence:

>1491_bases
ATGATACGGCGGCTGCTGCTCTGTTGCCGCGGCGAGATCGCGCTGCGCTTCATCCGCACTTGCCGCTTGATGGGTGTGGA
AACCGTGGCGGCTTATCCGGCGGAGGATGACGGCCACCCCTACGTTCTGGCCGCCGATCAGCGCTTTCCCATCGAGGCGG
CCAGCGCCGGCAGCGCGATGGCTGAAGTGCTGCAAGTCGCCCGCTTGGCGCGGGTGGACGCGATCGCGCCGGGCTATGGC
CCTCTGGCGGAAAACGCCGAATTCGCCGCGGCTTGCGCCGAGGCCGGTTTCATCTTCGTCGGTCCCAGGCCGGAGGCCAT
CCGGCAGTCCGGCGACAAGCTGCGCGCCCGCGAGGCCGCCTGCCTGGCCGGCGTGCCGGTGATCCCCGGCGGCCATCCTG
GGGACGGGCTGGAGCAATCGCTGGATCTGGCGCGGGAAATCGGCTTTCCGGTGCTGATCAAGGCGGTGATGGGAGGCGGC
GGCCGCGGCATACGGGTGGTGGAGGCGCCGGAGGCCTTCGCCGGCGCGCTCGATGAAGTGAGGAGTGAGGCGCTTTCCGC
ATTCGGCTGCGGCGATGTCTATCTCGAGCGGTTCCTCGGCACCGCCATCCGCCATATCGAGGTGCAGGTGCTGGGCGACA
AGCACGGCAATGTGCTGCATCTGGGCGACCGCGGCTGCAGCGTCCAGCGTCGGCGGCAAAAACTGGTGGAGGAAGCGCCG
GTGCCGGGACTGGACGCGGCGCTGAGGAACGAATTGCGGCTGGCGGCGCTGGCGATCGCGCGGCAGCTGGGCTACGACAG
CGCCGGCACCGTCGAATTCCTGGTTGCCGGCGACGGGCGTTTCTACTTCATCGAAATGAACGCGCGCATCCAGGTGGAGC
ATCCGGTGACGGAGGCGATCACCGGGATCGATCTGGTCGAGCAGATGATTCATGTCGCCGGCGGCGGACGTTTGTCGCTG
CGCCAGGATGACATCGAATTCCGCGGCCATGCGGTCGAATTCAGGCTCTGCGCCGAAGACCCGCGCAATCAGTTCTTTCC
GGCTGCCGGACGGGTCGCCAGCTATGCCGTGCCGGAGGGCGAGGGCGTGCGGCTGGATTCGGGCGTGGCCAGCGGCGTCG
CGCAGTCGCCGCGATTCGACAGCTTGTGCGCCAAGCTCATCGTCCATCAGTCCAGCCGTGAACGCGCGCTGGCGCGCGCC
TCGCATGCGCTGGGAGAGTTCCGCCTGTTCGGCTTTCCGACCAATGTCCCGTTCCATCACTGGTTGCTGGAGCAGCCGCA
GTTCCGCGACGGCAGCTACGACTTGTCGTTGCTGGAGGATTTCGAGCTGCCGCCGGAGCGACTGCATGCCGTCGACCGAA
GGGCGGTGTTTGCCGCGGCGCTGGCGACGGCGCTGGCTGGCGTGGCCGGGCGCAGGCCGGAGGGCTCGGCCGCGGTGTCG
GGCTGGAAACTGCGCAACGATCCGCTGCTGGCGGCCGGCGCGCGAGGCTAG

Upstream 100 bases:

>100_bases
GCGAAGTCTCGGTGGCGGGCGCCGTGACGGCGGGCGAGTTCACGCATGCGCACACCGTGCTGGGGCGCGGGCTGCACGGC
GACAATGCGGAGCGCAAGGC

Downstream 100 bases:

>100_bases
GAGGGGAGTGGATGAACGATAGAGTGGACAGAGAGGGCGGCGTGGCGTTGCGGTTGCACGCCGCTCTGCCCGCAGGCTGG
AGCGGCGGGATCGAACTGCG

Product: biotin carboxylase protein

Products: NA

Alternate protein names: Pyruvic carboxylase A [H]

Number of amino acids: Translated: 496; Mature: 496

Protein sequence:

>496_residues
MIRRLLLCCRGEIALRFIRTCRLMGVETVAAYPAEDDGHPYVLAADQRFPIEAASAGSAMAEVLQVARLARVDAIAPGYG
PLAENAEFAAACAEAGFIFVGPRPEAIRQSGDKLRAREAACLAGVPVIPGGHPGDGLEQSLDLAREIGFPVLIKAVMGGG
GRGIRVVEAPEAFAGALDEVRSEALSAFGCGDVYLERFLGTAIRHIEVQVLGDKHGNVLHLGDRGCSVQRRRQKLVEEAP
VPGLDAALRNELRLAALAIARQLGYDSAGTVEFLVAGDGRFYFIEMNARIQVEHPVTEAITGIDLVEQMIHVAGGGRLSL
RQDDIEFRGHAVEFRLCAEDPRNQFFPAAGRVASYAVPEGEGVRLDSGVASGVAQSPRFDSLCAKLIVHQSSRERALARA
SHALGEFRLFGFPTNVPFHHWLLEQPQFRDGSYDLSLLEDFELPPERLHAVDRRAVFAAALATALAGVAGRRPEGSAAVS
GWKLRNDPLLAAGARG

Sequences:

>Translated_496_residues
MIRRLLLCCRGEIALRFIRTCRLMGVETVAAYPAEDDGHPYVLAADQRFPIEAASAGSAMAEVLQVARLARVDAIAPGYG
PLAENAEFAAACAEAGFIFVGPRPEAIRQSGDKLRAREAACLAGVPVIPGGHPGDGLEQSLDLAREIGFPVLIKAVMGGG
GRGIRVVEAPEAFAGALDEVRSEALSAFGCGDVYLERFLGTAIRHIEVQVLGDKHGNVLHLGDRGCSVQRRRQKLVEEAP
VPGLDAALRNELRLAALAIARQLGYDSAGTVEFLVAGDGRFYFIEMNARIQVEHPVTEAITGIDLVEQMIHVAGGGRLSL
RQDDIEFRGHAVEFRLCAEDPRNQFFPAAGRVASYAVPEGEGVRLDSGVASGVAQSPRFDSLCAKLIVHQSSRERALARA
SHALGEFRLFGFPTNVPFHHWLLEQPQFRDGSYDLSLLEDFELPPERLHAVDRRAVFAAALATALAGVAGRRPEGSAAVS
GWKLRNDPLLAAGARG
>Mature_496_residues
MIRRLLLCCRGEIALRFIRTCRLMGVETVAAYPAEDDGHPYVLAADQRFPIEAASAGSAMAEVLQVARLARVDAIAPGYG
PLAENAEFAAACAEAGFIFVGPRPEAIRQSGDKLRAREAACLAGVPVIPGGHPGDGLEQSLDLAREIGFPVLIKAVMGGG
GRGIRVVEAPEAFAGALDEVRSEALSAFGCGDVYLERFLGTAIRHIEVQVLGDKHGNVLHLGDRGCSVQRRRQKLVEEAP
VPGLDAALRNELRLAALAIARQLGYDSAGTVEFLVAGDGRFYFIEMNARIQVEHPVTEAITGIDLVEQMIHVAGGGRLSL
RQDDIEFRGHAVEFRLCAEDPRNQFFPAAGRVASYAVPEGEGVRLDSGVASGVAQSPRFDSLCAKLIVHQSSRERALARA
SHALGEFRLFGFPTNVPFHHWLLEQPQFRDGSYDLSLLEDFELPPERLHAVDRRAVFAAALATALAGVAGRRPEGSAAVS
GWKLRNDPLLAAGARG

Specific function: Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second [H]

COG id: COG0439

COG function: function code I; Biotin carboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 biotin carboxylation domain [H]

Homologues:

Organism=Homo sapiens, GI116805327, Length=436, Percent_Identity=42.8899082568807, Blast_Score=358, Evalue=9e-99,
Organism=Homo sapiens, GI106049528, Length=457, Percent_Identity=43.9824945295405, Blast_Score=357, Evalue=2e-98,
Organism=Homo sapiens, GI106049295, Length=457, Percent_Identity=43.9824945295405, Blast_Score=357, Evalue=2e-98,
Organism=Homo sapiens, GI106049292, Length=457, Percent_Identity=43.9824945295405, Blast_Score=357, Evalue=2e-98,
Organism=Homo sapiens, GI189095269, Length=446, Percent_Identity=43.0493273542601, Blast_Score=320, Evalue=1e-87,
Organism=Homo sapiens, GI65506442, Length=446, Percent_Identity=43.0493273542601, Blast_Score=320, Evalue=2e-87,
Organism=Homo sapiens, GI295821183, Length=446, Percent_Identity=43.0493273542601, Blast_Score=320, Evalue=2e-87,
Organism=Homo sapiens, GI38679971, Length=514, Percent_Identity=29.3774319066148, Blast_Score=198, Evalue=1e-50,
Organism=Homo sapiens, GI38679974, Length=514, Percent_Identity=29.3774319066148, Blast_Score=198, Evalue=1e-50,
Organism=Homo sapiens, GI38679960, Length=514, Percent_Identity=29.3774319066148, Blast_Score=198, Evalue=1e-50,
Organism=Homo sapiens, GI38679977, Length=514, Percent_Identity=29.3774319066148, Blast_Score=198, Evalue=1e-50,
Organism=Homo sapiens, GI38679967, Length=514, Percent_Identity=29.3774319066148, Blast_Score=198, Evalue=1e-50,
Organism=Homo sapiens, GI134142062, Length=504, Percent_Identity=30.1587301587302, Blast_Score=192, Evalue=5e-49,
Organism=Escherichia coli, GI1789654, Length=444, Percent_Identity=42.5675675675676, Blast_Score=335, Evalue=5e-93,
Organism=Caenorhabditis elegans, GI71987519, Length=446, Percent_Identity=43.2735426008969, Blast_Score=332, Evalue=3e-91,
Organism=Caenorhabditis elegans, GI17562816, Length=461, Percent_Identity=42.0824295010846, Blast_Score=331, Evalue=4e-91,
Organism=Caenorhabditis elegans, GI17567343, Length=450, Percent_Identity=41.3333333333333, Blast_Score=314, Evalue=6e-86,
Organism=Caenorhabditis elegans, GI133931226, Length=487, Percent_Identity=29.1581108829569, Blast_Score=189, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI71997168, Length=432, Percent_Identity=29.1666666666667, Blast_Score=176, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI71997163, Length=431, Percent_Identity=29.6983758700696, Blast_Score=174, Evalue=7e-44,
Organism=Saccharomyces cerevisiae, GI6319695, Length=449, Percent_Identity=43.2071269487751, Blast_Score=326, Evalue=5e-90,
Organism=Saccharomyces cerevisiae, GI6321376, Length=497, Percent_Identity=41.6498993963783, Blast_Score=318, Evalue=9e-88,
Organism=Saccharomyces cerevisiae, GI6319685, Length=445, Percent_Identity=37.752808988764, Blast_Score=314, Evalue=2e-86,
Organism=Saccharomyces cerevisiae, GI6324343, Length=513, Percent_Identity=30.7992202729045, Blast_Score=198, Evalue=2e-51,
Organism=Saccharomyces cerevisiae, GI6323863, Length=471, Percent_Identity=30.7855626326964, Blast_Score=186, Evalue=7e-48,
Organism=Drosophila melanogaster, GI24651757, Length=500, Percent_Identity=41.6, Blast_Score=347, Evalue=1e-95,
Organism=Drosophila melanogaster, GI281363050, Length=451, Percent_Identity=45.0110864745011, Blast_Score=345, Evalue=6e-95,
Organism=Drosophila melanogaster, GI24652224, Length=451, Percent_Identity=45.0110864745011, Blast_Score=345, Evalue=6e-95,
Organism=Drosophila melanogaster, GI24652222, Length=451, Percent_Identity=45.0110864745011, Blast_Score=345, Evalue=6e-95,
Organism=Drosophila melanogaster, GI24652220, Length=451, Percent_Identity=45.0110864745011, Blast_Score=345, Evalue=6e-95,
Organism=Drosophila melanogaster, GI24652218, Length=451, Percent_Identity=45.0110864745011, Blast_Score=345, Evalue=6e-95,
Organism=Drosophila melanogaster, GI24652212, Length=451, Percent_Identity=45.0110864745011, Blast_Score=344, Evalue=6e-95,
Organism=Drosophila melanogaster, GI24652210, Length=451, Percent_Identity=45.0110864745011, Blast_Score=344, Evalue=6e-95,
Organism=Drosophila melanogaster, GI24652214, Length=451, Percent_Identity=45.0110864745011, Blast_Score=344, Evalue=6e-95,
Organism=Drosophila melanogaster, GI19921944, Length=451, Percent_Identity=45.0110864745011, Blast_Score=344, Evalue=6e-95,
Organism=Drosophila melanogaster, GI24652216, Length=451, Percent_Identity=45.0110864745011, Blast_Score=344, Evalue=6e-95,
Organism=Drosophila melanogaster, GI24651759, Length=457, Percent_Identity=41.5754923413567, Blast_Score=313, Evalue=1e-85,
Organism=Drosophila melanogaster, GI161076407, Length=498, Percent_Identity=29.3172690763052, Blast_Score=192, Evalue=4e-49,
Organism=Drosophila melanogaster, GI24586460, Length=498, Percent_Identity=29.3172690763052, Blast_Score=192, Evalue=4e-49,
Organism=Drosophila melanogaster, GI24586458, Length=498, Percent_Identity=29.3172690763052, Blast_Score=192, Evalue=5e-49,
Organism=Drosophila melanogaster, GI161076409, Length=498, Percent_Identity=29.3172690763052, Blast_Score=192, Evalue=5e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004549
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR011764
- InterPro:   IPR005482
- InterPro:   IPR005479
- InterPro:   IPR005481
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR011054 [H]

Pfam domain/function: PF02785 Biotin_carb_C; PF00289 CPSase_L_chain; PF02786 CPSase_L_D2 [H]

EC number: =6.4.1.1 [H]

Molecular weight: Translated: 53212; Mature: 53212

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: PS50975 ATP_GRASP ; PS00867 CPSASE_2 ; PS50979 BC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRRLLLCCRGEIALRFIRTCRLMGVETVAAYPAEDDGHPYVLAADQRFPIEAASAGSAM
CHHHHHHHHCCHHHHHHHHHHHHHCCHHHEECCCCCCCCCEEEECCCCCCCCCCCCHHHH
AEVLQVARLARVDAIAPGYGPLAENAEFAAACAEAGFIFVGPRPEAIRQSGDKLRAREAA
HHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCEEEECCCCHHHHCCCHHHHHHHHH
CLAGVPVIPGGHPGDGLEQSLDLAREIGFPVLIKAVMGGGGRGIRVVEAPEAFAGALDEV
HHCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHH
RSEALSAFGCGDVYLERFLGTAIRHIEVQVLGDKHGNVLHLGDRGCSVQRRRQKLVEEAP
HHHHHHHCCCHHHHHHHHHHHHHHEEEEEEEECCCCCEEEECCCCCCHHHHHHHHHHHCC
VPGLDAALRNELRLAALAIARQLGYDSAGTVEFLVAGDGRFYFIEMNARIQVEHPVTEAI
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCEEEEEEECCEEEECCCHHHHH
TGIDLVEQMIHVAGGGRLSLRQDDIEFRGHAVEFRLCAEDPRNQFFPAAGRVASYAVPEG
HHHHHHHHHHHHCCCCEEEEECCCHHCCCCEEEEEEECCCCCCCCCCCCCCHHEEECCCC
EGVRLDSGVASGVAQSPRFDSLCAKLIVHQSSRERALARASHALGEFRLFGFPTNVPFHH
CCEEECCCHHHCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCEEEEECCCCCCHHH
WLLEQPQFRDGSYDLSLLEDFELPPERLHAVDRRAVFAAALATALAGVAGRRPEGSAAVS
HHCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
GWKLRNDPLLAAGARG
CCEECCCCEEECCCCC
>Mature Secondary Structure
MIRRLLLCCRGEIALRFIRTCRLMGVETVAAYPAEDDGHPYVLAADQRFPIEAASAGSAM
CHHHHHHHHCCHHHHHHHHHHHHHCCHHHEECCCCCCCCCEEEECCCCCCCCCCCCHHHH
AEVLQVARLARVDAIAPGYGPLAENAEFAAACAEAGFIFVGPRPEAIRQSGDKLRAREAA
HHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCEEEECCCCHHHHCCCHHHHHHHHH
CLAGVPVIPGGHPGDGLEQSLDLAREIGFPVLIKAVMGGGGRGIRVVEAPEAFAGALDEV
HHCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHH
RSEALSAFGCGDVYLERFLGTAIRHIEVQVLGDKHGNVLHLGDRGCSVQRRRQKLVEEAP
HHHHHHHCCCHHHHHHHHHHHHHHEEEEEEEECCCCCEEEECCCCCCHHHHHHHHHHHCC
VPGLDAALRNELRLAALAIARQLGYDSAGTVEFLVAGDGRFYFIEMNARIQVEHPVTEAI
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCEEEEEEECCEEEECCCHHHHH
TGIDLVEQMIHVAGGGRLSLRQDDIEFRGHAVEFRLCAEDPRNQFFPAAGRVASYAVPEG
HHHHHHHHHHHHCCCCEEEEECCCHHCCCCEEEEEEECCCCCCCCCCCCCCHHEEECCCC
EGVRLDSGVASGVAQSPRFDSLCAKLIVHQSSRERALARASHALGEFRLFGFPTNVPFHH
CCEEECCCHHHCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCEEEEECCCCCCHHH
WLLEQPQFRDGSYDLSLLEDFELPPERLHAVDRRAVFAAALATALAGVAGRRPEGSAAVS
HHCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
GWKLRNDPLLAAGARG
CCEECCCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087; 11195096 [H]