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The map label for this gene is paaF [C]

Identifier: 34497218

GI number: 34497218

Start: 1918467

End: 1919255

Strand: Reverse

Name: paaF [C]

Synonym: CV_1763

Alternate gene names: 34497218

Gene position: 1919255-1918467 (Counterclockwise)

Preceding gene: 34497219

Following gene: 34497217

Centisome position: 40.4

GC content: 71.23

Gene sequence:

>789_bases
ATGAGCTACACCACGCTGCAAATCGAGCAAACCGGCAAGGTGGCCACCGTCTGGCTGAACCGGCCGGAGCTGCACAACGC
GCTGAACGAGGTGCTGATCGCCGAGCTGACCGAGGCGATGCGCCAGCTGAACCGCAACGAGGCCGTTCGCGTCATCGTGC
TGGCCGGCCGCGGCAAGAGCTTCTGCGCCGGCGCCGACCTGGACTGGATGCGTCGCGCCGCCGGCTACAGCGAGGCGGAA
AACCTGGCCGACGCGCAAAAGCTGGCCGCCATGCTGAAGGGCATCTACCGCAGCGCCAAGCCGGTGATCGCCCGCATCCA
CGGCGCGGCGATGGCCGGCGGCACCGGCCTGGCCGCGGTCTGCGACGTCGCCATCGCCACCGACGCCGCCAAATTCGCGC
TGACCGAGGTGCGGCTGGGCCTGGTCCCCGCCACCATCGGCCCCTACGTCGCCGACGCCATCGGCCCGCGCCAGGCCCGG
CGCTACTTCCTGTCCGCCGAGCGCATCACCGCCGGCACCGCGCTGAAGCTGGGCCTGATCCACGAACTGGTCAGCGAGGA
ACTGCTGGACGCGCGCGTGGCCGAGATCGCCGAGGAGCTGGCCAAGGGCGCGCCCGGCGCCTTGCGCGAGGCCAAGCTGC
TGCTGGCCGAGCTGCGCGAAGGCTCGCCCTGGGACGACCAGCTGCTGGAGCGCACCGCCGGCCGCATCGCCTCCATCCGC
GCCGGCGACGAGGCCCGCGAGGGGCTGGCTTCGTTCTTCGACAAACGCGCGCCGAAATGGCAGGAATAA

Upstream 100 bases:

>100_bases
GCCCAGACCCGCGACGTGTTGGCGCTGGCGCTGGAGGCCGCGCTGTCCGCCCCGGTTGAAGCCACGCGCTTCGGCGTGTT
CCGGATGTGAGGAGCGGATG

Downstream 100 bases:

>100_bases
CAAGGATAACGCCATGTTCAACAAGATCCTGATCGCCAACAGGGGTGAGATAGCCTGCCGCGTCATCAAGACCGCCAAGG
CCCTGGGCATCGCCACCGTC

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 262; Mature: 261

Protein sequence:

>262_residues
MSYTTLQIEQTGKVATVWLNRPELHNALNEVLIAELTEAMRQLNRNEAVRVIVLAGRGKSFCAGADLDWMRRAAGYSEAE
NLADAQKLAAMLKGIYRSAKPVIARIHGAAMAGGTGLAAVCDVAIATDAAKFALTEVRLGLVPATIGPYVADAIGPRQAR
RYFLSAERITAGTALKLGLIHELVSEELLDARVAEIAEELAKGAPGALREAKLLLAELREGSPWDDQLLERTAGRIASIR
AGDEAREGLASFFDKRAPKWQE

Sequences:

>Translated_262_residues
MSYTTLQIEQTGKVATVWLNRPELHNALNEVLIAELTEAMRQLNRNEAVRVIVLAGRGKSFCAGADLDWMRRAAGYSEAE
NLADAQKLAAMLKGIYRSAKPVIARIHGAAMAGGTGLAAVCDVAIATDAAKFALTEVRLGLVPATIGPYVADAIGPRQAR
RYFLSAERITAGTALKLGLIHELVSEELLDARVAEIAEELAKGAPGALREAKLLLAELREGSPWDDQLLERTAGRIASIR
AGDEAREGLASFFDKRAPKWQE
>Mature_261_residues
SYTTLQIEQTGKVATVWLNRPELHNALNEVLIAELTEAMRQLNRNEAVRVIVLAGRGKSFCAGADLDWMRRAAGYSEAEN
LADAQKLAAMLKGIYRSAKPVIARIHGAAMAGGTGLAAVCDVAIATDAAKFALTEVRLGLVPATIGPYVADAIGPRQARR
YFLSAERITAGTALKLGLIHELVSEELLDARVAEIAEELAKGAPGALREAKLLLAELREGSPWDDQLLERTAGRIASIRA
GDEAREGLASFFDKRAPKWQE

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=263, Percent_Identity=30.7984790874525, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI70995211, Length=276, Percent_Identity=27.8985507246377, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI31542718, Length=255, Percent_Identity=27.0588235294118, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI45643119, Length=212, Percent_Identity=25.9433962264151, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI260275230, Length=212, Percent_Identity=25.9433962264151, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI260274832, Length=212, Percent_Identity=25.9433962264151, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI37594471, Length=191, Percent_Identity=27.7486910994764, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI37594469, Length=191, Percent_Identity=27.7486910994764, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI4502327, Length=259, Percent_Identity=30.1158301158301, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI68989263, Length=178, Percent_Identity=30.8988764044944, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI20127408, Length=218, Percent_Identity=28.8990825688073, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI157694516, Length=219, Percent_Identity=24.2009132420091, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI213417737, Length=219, Percent_Identity=24.2009132420091, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI1787659, Length=265, Percent_Identity=30.9433962264151, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1788597, Length=257, Percent_Identity=27.6264591439689, Blast_Score=98, Evalue=6e-22,
Organism=Escherichia coli, GI87082183, Length=263, Percent_Identity=27.7566539923954, Blast_Score=97, Evalue=7e-22,
Organism=Escherichia coli, GI1787660, Length=263, Percent_Identity=30.7984790874525, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI221142681, Length=266, Percent_Identity=30.0751879699248, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1788682, Length=196, Percent_Identity=33.1632653061224, Blast_Score=79, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI25145438, Length=255, Percent_Identity=32.156862745098, Blast_Score=113, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17554946, Length=273, Percent_Identity=28.2051282051282, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17535521, Length=263, Percent_Identity=29.6577946768061, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17540714, Length=208, Percent_Identity=30.2884615384615, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17540306, Length=258, Percent_Identity=26.3565891472868, Blast_Score=87, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI17558304, Length=190, Percent_Identity=32.1052631578947, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17560910, Length=183, Percent_Identity=31.1475409836066, Blast_Score=85, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17534483, Length=282, Percent_Identity=26.241134751773, Blast_Score=85, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17508951, Length=148, Percent_Identity=35.8108108108108, Blast_Score=81, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17508953, Length=148, Percent_Identity=35.8108108108108, Blast_Score=81, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI25144276, Length=148, Percent_Identity=35.8108108108108, Blast_Score=81, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17536985, Length=241, Percent_Identity=29.045643153527, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17549921, Length=225, Percent_Identity=26.2222222222222, Blast_Score=74, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI25144160, Length=194, Percent_Identity=28.8659793814433, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI25144157, Length=194, Percent_Identity=28.8659793814433, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI25141363, Length=163, Percent_Identity=28.8343558282209, Blast_Score=65, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6320241, Length=211, Percent_Identity=30.8056872037915, Blast_Score=73, Evalue=4e-14,
Organism=Drosophila melanogaster, GI20129971, Length=263, Percent_Identity=30.4182509505703, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24653477, Length=263, Percent_Identity=30.4182509505703, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24653139, Length=254, Percent_Identity=27.1653543307087, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI19922422, Length=261, Percent_Identity=24.5210727969349, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24654903, Length=192, Percent_Identity=26.5625, Blast_Score=81, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24650670, Length=200, Percent_Identity=30, Blast_Score=78, Evalue=5e-15,
Organism=Drosophila melanogaster, GI19920382, Length=227, Percent_Identity=29.5154185022026, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI28571729, Length=150, Percent_Identity=29.3333333333333, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI28571730, Length=150, Percent_Identity=29.3333333333333, Blast_Score=69, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 28229; Mature: 28098

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYTTLQIEQTGKVATVWLNRPELHNALNEVLIAELTEAMRQLNRNEAVRVIVLAGRGKS
CCCEEEEEECCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCC
FCAGADLDWMRRAAGYSEAENLADAQKLAAMLKGIYRSAKPVIARIHGAAMAGGTGLAAV
CCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
CDVAIATDAAKFALTEVRLGLVPATIGPYVADAIGPRQARRYFLSAERITAGTALKLGLI
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHH
HELVSEELLDARVAEIAEELAKGAPGALREAKLLLAELREGSPWDDQLLERTAGRIASIR
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCC
AGDEAREGLASFFDKRAPKWQE
CCHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SYTTLQIEQTGKVATVWLNRPELHNALNEVLIAELTEAMRQLNRNEAVRVIVLAGRGKS
CCEEEEEECCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCC
FCAGADLDWMRRAAGYSEAENLADAQKLAAMLKGIYRSAKPVIARIHGAAMAGGTGLAAV
CCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
CDVAIATDAAKFALTEVRLGLVPATIGPYVADAIGPRQARRYFLSAERITAGTALKLGLI
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHH
HELVSEELLDARVAEIAEELAKGAPGALREAKLLLAELREGSPWDDQLLERTAGRIASIR
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCC
AGDEAREGLASFFDKRAPKWQE
CCHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA