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The map label for this gene is amiD [H]

Identifier: 34496764

GI number: 34496764

Start: 1379642

End: 1380541

Strand: Direct

Name: amiD [H]

Synonym: CV_1309

Alternate gene names: 34496764

Gene position: 1379642-1380541 (Clockwise)

Preceding gene: 34496763

Following gene: 34496765

Centisome position: 29.04

GC content: 67.11

Gene sequence:

>900_bases
ATGGCGAGCCGTAGGCAATTTCTGCGCCGGACGGGAATGATCGTCGCTGGCGGCGCGCTGCTGGCGGCGCCGGCCGCCCG
GGCGCAGGGCGAGATGATTGAGTACTGGATAGACGAATCCCGTTCGTCGGCCAACCAGGATTCCCGGATCCGCACGCTGG
TGTTCCACTACACCGCGGAAGACTTCGCAACCTCGCTCAAGCTGCTGACCGAACCGCAATACCGGACCAGCGCGCATTAC
TTGGTGCCGGATGCCGCGACGCTGTCCAGGCGGCCCGCCATTCTGCGCTTGGTGGAGGAGGAGCGGCGGGCCTGGCACGC
CGGCGACAGTTATTGGCGGGGGCAGCGTTATCTGAATGGCGCCTCGATAGGCGTGGAAATCGTCAATCGAGGCTATCCGT
CGCCGTTGCAGGACGATTGGCCGCCGATGCGGCGGGATTGGCAAGCGTTCGACGATGCGCAGATCGCGCAGGTCGGCAAG
CTGGCGGCCGGCATCGTCGCCCGCCACCGGATACAGCCCTGCGATGTCGTCGGACACGCCGATATCGCGCCGGGGCGGAA
GATGGACCCGGGGCCGAAGTTTCCCTGGGAGCGATTGCACCGGGAGTTCGGCGTCGGCGCCTGGCCGGATCCTGACGATG
TCCGCCGTTTCCTGGCGCTGCAACCGGGAACGCCGGATGCGGCGAGCTGGCAGCGGCGGCTGGCCGCCTATGGCTACGAT
GCGCCGCGGAGCGGCGAATGGGACGAGAAAACCAGGCACGCCATCCAGGCGTTCCAGATGCATTTTCGCCCGGCGCGTTA
CGACGGCGTGCCGGACGCGGAATCGTCGGCGATTCTGGATGCGCTGCTGGACAAGTATCTCGGCCCGGTCCCGGGCGGCG
AAGAGGGAGAGCATCCCTGA

Upstream 100 bases:

>100_bases
CATCCTTCATCGTGCGGCGGGCCTGCTTCGGGATCTTCATCTTGAACGCTTGGTCTGATTGCAACTGGATATATCGCATC
GTGTGGAAAGGTGAAAAGTC

Downstream 100 bases:

>100_bases
GAAGGGACCGCGGCGCGGGCATTCATCGCCTGTTGTTTACAGTTCATTGACGGTTACGGATGTGACGCGTAGCGCCGTTT
ACGGCGGCGGCGGCGGTTTG

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 299; Mature: 298

Protein sequence:

>299_residues
MASRRQFLRRTGMIVAGGALLAAPAARAQGEMIEYWIDESRSSANQDSRIRTLVFHYTAEDFATSLKLLTEPQYRTSAHY
LVPDAATLSRRPAILRLVEEERRAWHAGDSYWRGQRYLNGASIGVEIVNRGYPSPLQDDWPPMRRDWQAFDDAQIAQVGK
LAAGIVARHRIQPCDVVGHADIAPGRKMDPGPKFPWERLHREFGVGAWPDPDDVRRFLALQPGTPDAASWQRRLAAYGYD
APRSGEWDEKTRHAIQAFQMHFRPARYDGVPDAESSAILDALLDKYLGPVPGGEEGEHP

Sequences:

>Translated_299_residues
MASRRQFLRRTGMIVAGGALLAAPAARAQGEMIEYWIDESRSSANQDSRIRTLVFHYTAEDFATSLKLLTEPQYRTSAHY
LVPDAATLSRRPAILRLVEEERRAWHAGDSYWRGQRYLNGASIGVEIVNRGYPSPLQDDWPPMRRDWQAFDDAQIAQVGK
LAAGIVARHRIQPCDVVGHADIAPGRKMDPGPKFPWERLHREFGVGAWPDPDDVRRFLALQPGTPDAASWQRRLAAYGYD
APRSGEWDEKTRHAIQAFQMHFRPARYDGVPDAESSAILDALLDKYLGPVPGGEEGEHP
>Mature_298_residues
ASRRQFLRRTGMIVAGGALLAAPAARAQGEMIEYWIDESRSSANQDSRIRTLVFHYTAEDFATSLKLLTEPQYRTSAHYL
VPDAATLSRRPAILRLVEEERRAWHAGDSYWRGQRYLNGASIGVEIVNRGYPSPLQDDWPPMRRDWQAFDDAQIAQVGKL
AAGIVARHRIQPCDVVGHADIAPGRKMDPGPKFPWERLHREFGVGAWPDPDDVRRFLALQPGTPDAASWQRRLAAYGYDA
PRSGEWDEKTRHAIQAFQMHFRPARYDGVPDAESSAILDALLDKYLGPVPGGEEGEHP

Specific function: Unknown

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1787092, Length=255, Percent_Identity=46.6666666666667, Blast_Score=193, Evalue=1e-50,
Organism=Escherichia coli, GI1786300, Length=138, Percent_Identity=30.4347826086957, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502
- InterPro:   IPR002477 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 33623; Mature: 33492

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASRRQFLRRTGMIVAGGALLAAPAARAQGEMIEYWIDESRSSANQDSRIRTLVFHYTAE
CCHHHHHHHHCCCEEECCHHHHCCHHHHCCHHHHHHHHHHHCCCCCCCCEEEEEEEECHH
DFATSLKLLTEPQYRTSAHYLVPDAATLSRRPAILRLVEEERRAWHAGDSYWRGQRYLNG
HHHHHHHHHCCCCHHCCCCEECCCHHHHHCCHHHHHHHHHHHHHHCCCCCHHHCHHHCCC
ASIGVEIVNRGYPSPLQDDWPPMRRDWQAFDDAQIAQVGKLAAGIVARHRIQPCDVVGHA
CHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
DIAPGRKMDPGPKFPWERLHREFGVGAWPDPDDVRRFLALQPGTPDAASWQRRLAAYGYD
CCCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHCCC
APRSGEWDEKTRHAIQAFQMHFRPARYDGVPDAESSAILDALLDKYLGPVPGGEEGEHP
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
ASRRQFLRRTGMIVAGGALLAAPAARAQGEMIEYWIDESRSSANQDSRIRTLVFHYTAE
CHHHHHHHHCCCEEECCHHHHCCHHHHCCHHHHHHHHHHHCCCCCCCCEEEEEEEECHH
DFATSLKLLTEPQYRTSAHYLVPDAATLSRRPAILRLVEEERRAWHAGDSYWRGQRYLNG
HHHHHHHHHCCCCHHCCCCEECCCHHHHHCCHHHHHHHHHHHHHHCCCCCHHHCHHHCCC
ASIGVEIVNRGYPSPLQDDWPPMRRDWQAFDDAQIAQVGKLAAGIVARHRIQPCDVVGHA
CHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
DIAPGRKMDPGPKFPWERLHREFGVGAWPDPDDVRRFLALQPGTPDAASWQRRLAAYGYD
CCCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHCCC
APRSGEWDEKTRHAIQAFQMHFRPARYDGVPDAESSAILDALLDKYLGPVPGGEEGEHP
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]