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The map label for this gene is rnhA

Identifier: 34496711

GI number: 34496711

Start: 1321674

End: 1322117

Strand: Direct

Name: rnhA

Synonym: CV_1256

Alternate gene names: 34496711

Gene position: 1321674-1322117 (Clockwise)

Preceding gene: 34496710

Following gene: 34496712

Centisome position: 27.82

GC content: 64.19

Gene sequence:

>444_bases
ATGACGACAGAAGACAGAGTCGAGATCTATACCGACGGCGCCTGCAAGGGCAACCCGGGGCCCGGGGGCTGGGGCGCGCT
GATGCGCTACAAGGGCAAGGAGAAGGAGTTGTTCGGCGGCGAGCGGGGCACCACCAACAACCGCATGGAGATCATGGCCG
TGATCCGGGCGCTGGCCGCGTTGAACCGGCCGTGCAAGGTGGTGGTCTACACCGACTCCCAGTACGTGCAGAAGGGCATT
TCCGAATGGATACACGGCTGGAAGGCGCGCGGCTGGAAAACCGCGGCCAAGGAGCCGGTCAAGAACGCCGATTTGTGGCA
GCAGCTGGACGCCGAGCGCAACCGACATCTGGACGTGGAATGGCGCTGGGTCAAGGGCCATGCCGGCCATGAATTCAACG
AGAGGGCCGACCAGCTCGCCAACAAGGGCGTGGAGTCGGTCTGA

Upstream 100 bases:

>100_bases
GCGCTGGGCGTGGTGGCGGGCAATGAGCGCCAGCACACGCACAAGCGGCCGGAAACCACCGAGCGGGACGGCGCATCGTC
CTGCCAATGAACGAGACAGC

Downstream 100 bases:

>100_bases
ACAGTAGACACGCCGCGGCGCCGTCGATACGGCGCCGCGGGCAGGACGCAGCATGAGACAGATTATTCTCGATACCGAAA
CCACCGGTCTCGATCCGCAG

Product: ribonuclease H

Products: NA

Alternate protein names: RNase H

Number of amino acids: Translated: 147; Mature: 146

Protein sequence:

>147_residues
MTTEDRVEIYTDGACKGNPGPGGWGALMRYKGKEKELFGGERGTTNNRMEIMAVIRALAALNRPCKVVVYTDSQYVQKGI
SEWIHGWKARGWKTAAKEPVKNADLWQQLDAERNRHLDVEWRWVKGHAGHEFNERADQLANKGVESV

Sequences:

>Translated_147_residues
MTTEDRVEIYTDGACKGNPGPGGWGALMRYKGKEKELFGGERGTTNNRMEIMAVIRALAALNRPCKVVVYTDSQYVQKGI
SEWIHGWKARGWKTAAKEPVKNADLWQQLDAERNRHLDVEWRWVKGHAGHEFNERADQLANKGVESV
>Mature_146_residues
TTEDRVEIYTDGACKGNPGPGGWGALMRYKGKEKELFGGERGTTNNRMEIMAVIRALAALNRPCKVVVYTDSQYVQKGIS
EWIHGWKARGWKTAAKEPVKNADLWQQLDAERNRHLDVEWRWVKGHAGHEFNERADQLANKGVESV

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0328

COG function: function code L; Ribonuclease HI

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RNase H domain

Homologues:

Organism=Homo sapiens, GI21359816, Length=146, Percent_Identity=36.986301369863, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1786408, Length=135, Percent_Identity=62.962962962963, Blast_Score=185, Evalue=1e-48,
Organism=Caenorhabditis elegans, GI115532542, Length=143, Percent_Identity=41.958041958042, Blast_Score=95, Evalue=1e-20,
Organism=Drosophila melanogaster, GI45552509, Length=149, Percent_Identity=38.255033557047, Blast_Score=89, Evalue=1e-18,
Organism=Drosophila melanogaster, GI17137476, Length=149, Percent_Identity=38.255033557047, Blast_Score=89, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH_CHRVO (Q7NYL8)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_900926.1
- HSSP:   P0A7Y4
- ProteinModelPortal:   Q7NYL8
- SMR:   Q7NYL8
- GeneID:   2550711
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_1256
- NMPDR:   fig|243365.1.peg.1256
- HOGENOM:   HBG742437
- OMA:   NGWRTAD
- PhylomeDB:   Q7NYL8
- BioCyc:   CVIO243365:CV_1256-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00042
- InterPro:   IPR022892
- InterPro:   IPR012337
- InterPro:   IPR002156

Pfam domain/function: PF00075 RnaseH; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 16669; Mature: 16538

Theoretical pI: Translated: 9.12; Mature: 9.12

Prosite motif: PS50879 RNASE_H

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTEDRVEIYTDGACKGNPGPGGWGALMRYKGKEKELFGGERGTTNNRMEIMAVIRALAA
CCCCCCEEEEECCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
LNRPCKVVVYTDSQYVQKGISEWIHGWKARGWKTAAKEPVKNADLWQQLDAERNRHLDVE
CCCCCEEEEEECHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCHHHHHHHHHHCCCCCCEE
WRWVKGHAGHEFNERADQLANKGVESV
EEEECCCCCCCHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TTEDRVEIYTDGACKGNPGPGGWGALMRYKGKEKELFGGERGTTNNRMEIMAVIRALAA
CCCCCEEEEECCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
LNRPCKVVVYTDSQYVQKGISEWIHGWKARGWKTAAKEPVKNADLWQQLDAERNRHLDVE
CCCCCEEEEEECHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCHHHHHHHHHHCCCCCCEE
WRWVKGHAGHEFNERADQLANKGVESV
EEEECCCCCCCHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14500782