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The map label for this gene is pleD [H]

Identifier: 34496267

GI number: 34496267

Start: 836929

End: 838080

Strand: Reverse

Name: pleD [H]

Synonym: CV_0812

Alternate gene names: 34496267

Gene position: 838080-836929 (Counterclockwise)

Preceding gene: 34496276

Following gene: 34496266

Centisome position: 17.64

GC content: 68.75

Gene sequence:

>1152_bases
TTGGAGCTGCAGCACGGCCAGCAGCGCCACATCTTCGAAATCCGCCTGTTCCCGGAGTTCGACAAGGCCGGCATGCTCAA
ATCCATCCTGTCGGTGGACCGCGACGTCACCGCCACCCGCCAGGGCGAGCGGCTGCTGGCCGAGGAAAACGAGGTGCTGG
AAATGATCGCCGGCAACCATCCGTTGCCGCAAACGCTGGAACAGATCTGCCAGATGATGGAGTCGCAGCAGCCGGGCTCG
ATGTGCAGCGTGCTGCTGCTGGAGGAGGATGGCCAGTCGCTGCGCTTCGCCGCCGGCCTCAGCCTGCCGCCGGCCTACCG
CAAGCTGTCCGAACGCGTGGCGGTCGGCCCCAGCGTCGGCTCCTGCGGCACCGCCGCGCACTGGAAGCGCACCATCGTCG
TCGACGACATCCAGACCAGCCCGCTGTGGGCGCACGCGCTGCACAAGGTGCAGCCCTTCGGCCTGCGCGCCTGCTGGTCG
ATGCCGATCTTCTCCAGCGACCGCCAGCTGCTTGGCACGCTGGCCACCTATTACCGCGAGCCGCGCAGCCCGTCCCAGGA
AGAGCTGGCGCTGGCCCAGCGCATCACCCGCATCATCGCCATCGCGCTGCAGCGGGACGGCCACGAAAAGCAACTGTACC
GGCTGGCCACCCAGGACGGACTGACCGAGCTGAACAACCGCCGCCAGTTCATCGAGCTGGCCGACCGGGAGATCGAGCGC
AGCCGCCGCCACGGCAGCCGGATGAGCGTGCTGATGATGGACCTGGACCATTTCAAGGACATCAACGACCACTACGGCCA
CGCCGTCGGCGACGAAGTGATACGCCACTTCTCCCAGGTCTGCCGCGAAGTGCTGCGCGCCAGCGATCTGTGCGGCCGCC
TGGGCGGCGAGGAGTTCGCCGCCGTGCTGCCGGACACCGGCCTGGACGACGCGCGCCAGGTGGCCGAACGCCTGCGCAGC
CTGGCCTCCTCCGCCAGACTGTCCGCCGTGTCGGGATCCTTGGGCTACACCGTCAGCATCGGCGTCGCCGAGCGCCATGC
CGACGAAACCGACATCGACGAAGTGCTGGGCCGCGCCGACAAGCAGCTGTACCAGGCCAAGCGCGACGGCCGCAACCGCG
TCAGCGGCGCGGCGCCCGCCCATTGCGGCTAG

Upstream 100 bases:

>100_bases
CAAGACGCCGCGCCAGAAGGGCTGGTCGGACGAGGCCAGCGCGCGCTTCGAACAGGAATGCCAGCTGTTGATCAACAACT
GGGAGGCGCGCCGCTTCGAG

Downstream 100 bases:

>100_bases
AATGCCGGCTTCGACCGCATCCCTCTGCTTTCCCGTGAACCGCTGGCACACCGACTACCTGTCCCATCCGCTGTACCGCC
CGACGCGGCGCTTCCTGCCC

Product: hypothetical protein

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 383; Mature: 383

Protein sequence:

>383_residues
MELQHGQQRHIFEIRLFPEFDKAGMLKSILSVDRDVTATRQGERLLAEENEVLEMIAGNHPLPQTLEQICQMMESQQPGS
MCSVLLLEEDGQSLRFAAGLSLPPAYRKLSERVAVGPSVGSCGTAAHWKRTIVVDDIQTSPLWAHALHKVQPFGLRACWS
MPIFSSDRQLLGTLATYYREPRSPSQEELALAQRITRIIAIALQRDGHEKQLYRLATQDGLTELNNRRQFIELADREIER
SRRHGSRMSVLMMDLDHFKDINDHYGHAVGDEVIRHFSQVCREVLRASDLCGRLGGEEFAAVLPDTGLDDARQVAERLRS
LASSARLSAVSGSLGYTVSIGVAERHADETDIDEVLGRADKQLYQAKRDGRNRVSGAAPAHCG

Sequences:

>Translated_383_residues
MELQHGQQRHIFEIRLFPEFDKAGMLKSILSVDRDVTATRQGERLLAEENEVLEMIAGNHPLPQTLEQICQMMESQQPGS
MCSVLLLEEDGQSLRFAAGLSLPPAYRKLSERVAVGPSVGSCGTAAHWKRTIVVDDIQTSPLWAHALHKVQPFGLRACWS
MPIFSSDRQLLGTLATYYREPRSPSQEELALAQRITRIIAIALQRDGHEKQLYRLATQDGLTELNNRRQFIELADREIER
SRRHGSRMSVLMMDLDHFKDINDHYGHAVGDEVIRHFSQVCREVLRASDLCGRLGGEEFAAVLPDTGLDDARQVAERLRS
LASSARLSAVSGSLGYTVSIGVAERHADETDIDEVLGRADKQLYQAKRDGRNRVSGAAPAHCG
>Mature_383_residues
MELQHGQQRHIFEIRLFPEFDKAGMLKSILSVDRDVTATRQGERLLAEENEVLEMIAGNHPLPQTLEQICQMMESQQPGS
MCSVLLLEEDGQSLRFAAGLSLPPAYRKLSERVAVGPSVGSCGTAAHWKRTIVVDDIQTSPLWAHALHKVQPFGLRACWS
MPIFSSDRQLLGTLATYYREPRSPSQEELALAQRITRIIAIALQRDGHEKQLYRLATQDGLTELNNRRQFIELADREIER
SRRHGSRMSVLMMDLDHFKDINDHYGHAVGDEVIRHFSQVCREVLRASDLCGRLGGEEFAAVLPDTGLDDARQVAERLRS
LASSARLSAVSGSLGYTVSIGVAERHADETDIDEVLGRADKQLYQAKRDGRNRVSGAAPAHCG

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI87081881, Length=169, Percent_Identity=44.3786982248521, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1786584, Length=171, Percent_Identity=36.8421052631579, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI145693134, Length=157, Percent_Identity=41.4012738853503, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI87082007, Length=169, Percent_Identity=40.8284023668639, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1787262, Length=165, Percent_Identity=33.9393939393939, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1787802, Length=170, Percent_Identity=33.5294117647059, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1788381, Length=176, Percent_Identity=33.5227272727273, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1788085, Length=182, Percent_Identity=30.7692307692308, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1787816, Length=158, Percent_Identity=31.6455696202532, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI87081977, Length=237, Percent_Identity=28.6919831223629, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1787541, Length=172, Percent_Identity=29.0697674418605, Blast_Score=75, Evalue=9e-15,
Organism=Escherichia coli, GI1787056, Length=166, Percent_Identity=28.9156626506024, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI87081974, Length=155, Percent_Identity=28.3870967741935, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1788956, Length=172, Percent_Identity=30.8139534883721, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 42756; Mature: 42756

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS50113 PAC ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELQHGQQRHIFEIRLFPEFDKAGMLKSILSVDRDVTATRQGERLLAEENEVLEMIAGNH
CCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHHHCCC
PLPQTLEQICQMMESQQPGSMCSVLLLEEDGQSLRFAAGLSLPPAYRKLSERVAVGPSVG
CCHHHHHHHHHHHHCCCCCCCEEEEEEECCCCEEEEECCCCCCHHHHHHHHHHCCCCCCC
SCGTAAHWKRTIVVDDIQTSPLWAHALHKVQPFGLRACWSMPIFSSDRQLLGTLATYYRE
CCCCHHHCEEEEEEECCCCCCHHHHHHHHCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHC
PRSPSQEELALAQRITRIIAIALQRDGHEKQLYRLATQDGLTELNNRRQFIELADREIER
CCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SRRHGSRMSVLMMDLDHFKDINDHYGHAVGDEVIRHFSQVCREVLRASDLCGRLGGEEFA
HHHCCCHHHHHHHHHHHHHCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
AVLPDTGLDDARQVAERLRSLASSARLSAVSGSLGYTVSIGVAERHADETDIDEVLGRAD
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECHHHHCCCCCCHHHHHHHHH
KQLYQAKRDGRNRVSGAAPAHCG
HHHHHHHHCCCHHCCCCCCCCCC
>Mature Secondary Structure
MELQHGQQRHIFEIRLFPEFDKAGMLKSILSVDRDVTATRQGERLLAEENEVLEMIAGNH
CCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHHHCCC
PLPQTLEQICQMMESQQPGSMCSVLLLEEDGQSLRFAAGLSLPPAYRKLSERVAVGPSVG
CCHHHHHHHHHHHHCCCCCCCEEEEEEECCCCEEEEECCCCCCHHHHHHHHHHCCCCCCC
SCGTAAHWKRTIVVDDIQTSPLWAHALHKVQPFGLRACWSMPIFSSDRQLLGTLATYYRE
CCCCHHHCEEEEEEECCCCCCHHHHHHHHCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHC
PRSPSQEELALAQRITRIIAIALQRDGHEKQLYRLATQDGLTELNNRRQFIELADREIER
CCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SRRHGSRMSVLMMDLDHFKDINDHYGHAVGDEVIRHFSQVCREVLRASDLCGRLGGEEFA
HHHCCCHHHHHHHHHHHHHCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
AVLPDTGLDDARQVAERLRSLASSARLSAVSGSLGYTVSIGVAERHADETDIDEVLGRAD
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECHHHHCCCCCCHHHHHHHHH
KQLYQAKRDGRNRVSGAAPAHCG
HHHHHHHHCCCHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA