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The map label for this gene is emrA [H]

Identifier: 34496222

GI number: 34496222

Start: 788396

End: 789544

Strand: Reverse

Name: emrA [H]

Synonym: CV_0767

Alternate gene names: 34496222

Gene position: 789544-788396 (Counterclockwise)

Preceding gene: 34496223

Following gene: 34496221

Centisome position: 16.62

GC content: 69.54

Gene sequence:

>1149_bases
ATGGATCAGAAACTGGAAACCGCGAAGACTCGCAAACGCAACCTGCTGGTGGCCACCACCCTGTTCGCCGCCATCGCCAT
CGGCTACGGCGCCTATTGGGCGCTGGTGCTGAGCCACCAGGAAGACACCGACGACGCCTATGTCGGCGGCCACCTGGTTC
AGGTCACCCCGGAAGTGGGCGGCACCGTCGCCAAGATTCTGGTCGACGACACCGTGGCGGTGAAGGCCGGCCAGGTGCTG
GTCAGCTATGACAACAGCGACGCCCAGCTGGCCTTCGAGCGCGCCCGCAACGAATTCGCCCAGACCGTGCGCCAGACCCG
CCAGCTGATGGCCAACAGCCAGCAGCTGGACGCCCAGGTGGCGCTGCGCCAGGCCGAGCTGTCCCGCGCCGAATCCGACC
TGAAGCGCCGCAAGCTGCTGGCCGGCAGCGAGGCGATGTCCGCCGAGGAGCTGGGCCACGCGAGCGACGCCGTCGCCGCC
GCCCAGGCCGCGCTGGCCGCCGCGCAGGAACAGGCCAAGGCCGGCCTGGCGCTGGTGGGCAAGGACGAGCTGGCGCACCA
GCCCGCGGTGAAGGCCGCCGCCAGCCGCCTGAAGGAAGCCTGGCTCGCGCTGCAGCGCACCGAAATCAAGTCGCCGGTCA
ACGGCACCGTCGCCCGCCGCAACGTGCAGGTCGGCCAGCGCGTCGCCGCCGGCACGCCGCTGATGGCCGTGGTGCCGCTG
GAGCATCTGTGGGTGGACGCCAACTTCAAGGAAGGCCAACTGGCCAAGATCCGCATCGGCCAGCCGGTGGAGCTGAAGTC
CGACCTGTACGGCGGCAAGGTGGTCTACCACGGCAAGGTGCAGGGCCTGTCTGCCGGCACCGGCAGCGCCTTCTCGCTGC
TGCCGGCGCAGAACGCCACCGGCAACTGGATCAAGGTGGTGCAGCGCGTGCCGGTTCGCATCGCGCTCGACCCCAAGGAT
CTGCAGGAGCACCCGCTGCGCGTCGGCCTGTCGATCGACGCGGTGGTGGACACCAGCGACCAGGACGGCAAATCGCTGGC
CGAAGCCGCCCCGGCGACCGTGGTCAGCGAACACAACGGCACCCTGCCCGACCTGAAGCAGGCCGACAAGCTGGTCGCAG
AAATCCTGGCCGCCAACGCCGGCAAGTAA

Upstream 100 bases:

>100_bases
CGACGCCCAGGCCGCCAACCGCCTGGCCTGGGCCTCCCTGAACACCGCATTGGGCGGCGGCTTCGACAACCAGGCCGCCA
GCCGCTAAGGACGCACGAAA

Downstream 100 bases:

>100_bases
GGACGCCCACCCATGAACCATCCGCCACTGAGCGGCTCGAAACTGGTTTGGATCACGCTGGCGTTGTCGATGTCGGTGTT
CATGCAAGTGCTGGACACCA

Product: multidrug resistance secretion protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 382; Mature: 382

Protein sequence:

>382_residues
MDQKLETAKTRKRNLLVATTLFAAIAIGYGAYWALVLSHQEDTDDAYVGGHLVQVTPEVGGTVAKILVDDTVAVKAGQVL
VSYDNSDAQLAFERARNEFAQTVRQTRQLMANSQQLDAQVALRQAELSRAESDLKRRKLLAGSEAMSAEELGHASDAVAA
AQAALAAAQEQAKAGLALVGKDELAHQPAVKAAASRLKEAWLALQRTEIKSPVNGTVARRNVQVGQRVAAGTPLMAVVPL
EHLWVDANFKEGQLAKIRIGQPVELKSDLYGGKVVYHGKVQGLSAGTGSAFSLLPAQNATGNWIKVVQRVPVRIALDPKD
LQEHPLRVGLSIDAVVDTSDQDGKSLAEAAPATVVSEHNGTLPDLKQADKLVAEILAANAGK

Sequences:

>Translated_382_residues
MDQKLETAKTRKRNLLVATTLFAAIAIGYGAYWALVLSHQEDTDDAYVGGHLVQVTPEVGGTVAKILVDDTVAVKAGQVL
VSYDNSDAQLAFERARNEFAQTVRQTRQLMANSQQLDAQVALRQAELSRAESDLKRRKLLAGSEAMSAEELGHASDAVAA
AQAALAAAQEQAKAGLALVGKDELAHQPAVKAAASRLKEAWLALQRTEIKSPVNGTVARRNVQVGQRVAAGTPLMAVVPL
EHLWVDANFKEGQLAKIRIGQPVELKSDLYGGKVVYHGKVQGLSAGTGSAFSLLPAQNATGNWIKVVQRVPVRIALDPKD
LQEHPLRVGLSIDAVVDTSDQDGKSLAEAAPATVVSEHNGTLPDLKQADKLVAEILAANAGK
>Mature_382_residues
MDQKLETAKTRKRNLLVATTLFAAIAIGYGAYWALVLSHQEDTDDAYVGGHLVQVTPEVGGTVAKILVDDTVAVKAGQVL
VSYDNSDAQLAFERARNEFAQTVRQTRQLMANSQQLDAQVALRQAELSRAESDLKRRKLLAGSEAMSAEELGHASDAVAA
AQAALAAAQEQAKAGLALVGKDELAHQPAVKAAASRLKEAWLALQRTEIKSPVNGTVARRNVQVGQRVAAGTPLMAVVPL
EHLWVDANFKEGQLAKIRIGQPVELKSDLYGGKVVYHGKVQGLSAGTGSAFSLLPAQNATGNWIKVVQRVPVRIALDPKD
LQEHPLRVGLSIDAVVDTSDQDGKSLAEAAPATVVSEHNGTLPDLKQADKLVAEILAANAGK

Specific function: The emr locus confers resistance to substances of high hydrophobicity. EmrA probably participate in a transport system to extrude toxins and drugs from the cell [H]

COG id: COG1566

COG function: function code V; Multidrug resistance efflux pump

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789041, Length=379, Percent_Identity=51.1873350923483, Blast_Score=372, Evalue=1e-104,
Organism=Escherichia coli, GI1788711, Length=346, Percent_Identity=49.7109826589595, Blast_Score=330, Evalue=1e-91,
Organism=Escherichia coli, GI1790519, Length=306, Percent_Identity=31.0457516339869, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI87081951, Length=311, Percent_Identity=27.9742765273312, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1789637, Length=333, Percent_Identity=28.8288288288288, Blast_Score=85, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005694
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 40567; Mature: 40567

Theoretical pI: Translated: 7.32; Mature: 7.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDQKLETAKTRKRNLLVATTLFAAIAIGYGAYWALVLSHQEDTDDAYVGGHLVQVTPEVG
CCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCEECCEEEEECCCCC
GTVAKILVDDTVAVKAGQVLVSYDNSDAQLAFERARNEFAQTVRQTRQLMANSQQLDAQV
CCEEEEEECCCEEEECCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
ALRQAELSRAESDLKRRKLLAGSEAMSAEELGHASDAVAAAQAALAAAQEQAKAGLALVG
HHHHHHHHHHHHHHHHHHHHCCCHHHCHHHHCCHHHHHHHHHHHHHHHHHHHHCCEEEEE
KDELAHQPAVKAAASRLKEAWLALQRTEIKSPVNGTVARRNVQVGQRVAAGTPLMAVVPL
CHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCEEEEECH
EHLWVDANFKEGQLAKIRIGQPVELKSDLYGGKVVYHGKVQGLSAGTGSAFSLLPAQNAT
HHEEEECCCCCCCEEEEECCCCCCHHHCCCCCEEEEECEECCCCCCCCCCEEEECCCCCC
GNWIKVVQRVPVRIALDPKDLQEHPLRVGLSIDAVVDTSDQDGKSLAEAAPATVVSEHNG
CHHHHHHHHCCEEEEECHHHHHCCCEEECEEEEEEEECCCCCCHHHHHHCCCEEEECCCC
TLPDLKQADKLVAEILAANAGK
CCCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MDQKLETAKTRKRNLLVATTLFAAIAIGYGAYWALVLSHQEDTDDAYVGGHLVQVTPEVG
CCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCEECCEEEEECCCCC
GTVAKILVDDTVAVKAGQVLVSYDNSDAQLAFERARNEFAQTVRQTRQLMANSQQLDAQV
CCEEEEEECCCEEEECCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
ALRQAELSRAESDLKRRKLLAGSEAMSAEELGHASDAVAAAQAALAAAQEQAKAGLALVG
HHHHHHHHHHHHHHHHHHHHCCCHHHCHHHHCCHHHHHHHHHHHHHHHHHHHHCCEEEEE
KDELAHQPAVKAAASRLKEAWLALQRTEIKSPVNGTVARRNVQVGQRVAAGTPLMAVVPL
CHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCEEEEECH
EHLWVDANFKEGQLAKIRIGQPVELKSDLYGGKVVYHGKVQGLSAGTGSAFSLLPAQNAT
HHEEEECCCCCCCEEEEECCCCCCHHHCCCCCEEEEECEECCCCCCCCCCEEEECCCCCC
GNWIKVVQRVPVRIALDPKDLQEHPLRVGLSIDAVVDTSDQDGKSLAEAAPATVVSEHNG
CHHHHHHHHCCEEEEECHHHHHCCCEEECEEEEEEEECCCCCCHHHHHHCCCEEEECCCC
TLPDLKQADKLVAEILAANAGK
CCCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1409590; 9205837; 9278503; 8203018 [H]