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The map label for this gene is emrB [H]

Identifier: 34496221

GI number: 34496221

Start: 786851

End: 788326

Strand: Reverse

Name: emrB [H]

Synonym: CV_0766

Alternate gene names: 34496221

Gene position: 788326-786851 (Counterclockwise)

Preceding gene: 34496222

Following gene: 34496218

Centisome position: 16.59

GC content: 65.38

Gene sequence:

>1476_bases
ATGTCGGTGTTCATGCAAGTGCTGGACACCACCATCGCCAACGTGGCGCTGCCCACCATCTCGGGCAACCTCGGCGCCGC
CACCAGCCAGGGCACCTGGGTGATCACCTCGTTCGGCGTCGCCAACGCCATCGCCGTGCCGCTGACCGGCTGGCTGGCCA
AGCGCTTCGGCGAAGTGAAGCTGTTCACCGCGTCCACGCTGCTGTTCGTGCTGACCTCCTGGCTGTGCGGCATGTCGGGC
AGCCTGGAAATGCTGATCTTCTTCCGCGTGCTGCAAGGCGCGGTGGCCGGTCCGATGATCCCGCTGTCGCAGAGCCTGCT
GCTGTCCTGCTATCCGGCCGCCAAAAAGAGCATGGCGCTGGCGCTATGGGCGATGACGGTGATCGTCGCGCCGGTGTTCG
GGCCCATCCTGGGCGGCGTGATCAGCGACAACTGGCACTGGGGCTGGATCTTCTTCATCAACGTGCCGGTGGGCCTCGCG
GCCGCCTTCATCTCCTGGCGCATCTTGAAGAGCCGCGAGACCGACACCTTCGACCTGCCCATCGACAAGCTCGGCCTGGC
CTTGCTGGTGATCGGCGTCGGCTCGCTGCAGATGGTGCTGGACCGCGGCAAGGAACTGGACTGGTTCAACTCCGCCGAGA
TCGTCACCCTGGCGGTGATCGCCTTCCTGGCGCTGACCTATCTAGTGATCTGGGAGCTGGGCGAGGAACACCCCATCGTC
GACCTGTCGCTGTTCAGCCAGCGCAACTTCACCGTCGGCACCGTCGCCATCAGCCTGGGTTTCATGCTGTACTTCGGCGC
CGTGGTGCTGCTGCCCTTGATGCTGCAGACCCAGCTGGGCTACACCGCCACCTGGGCCGGCCTCGCCGCGGCGCCGATCG
GCCTGATCCCGGTGGTGCTGTCGCCGATCATCGGCAAGAACGCCCACCGCTGGGACATGCGCTGGATCGTCACCGTCAGC
TTCAGCGTCTACGCCATCTGCTTCTTCTGGCGCAGCACCTTCAACACCGACATGGACTTCTCCTACGTGGTGTGGCCGCA
GGTGGTGCAGGGCATAGGCGTGGCGTGCTTCTTCATGCCGCTGACCACGATCACGCTGTCCGGCCTGCATCCGTCGCGGA
TCGCCAGCGCGTCCAGCCTGTCGAACTTCCTGCGCATCCTGGCCGGCAGCATCGGCACCTCGATCACCACCACGATGTGG
GACCGCCGCGAGGCGCAGCACCACTCCAAGCTGACCGAGCATGTGACCCAGTACGATCCGGCCTCGGTGTCGTGGTTCGA
CACCATGGGCAAGCTGGGCCTGGGCCCCAGCCAGCAGCACGCGCTGACCCAGCTGCAAATCACCAAGCAGGGCTACATCA
TGGCCGCCAACGAGATCTTCTGGGTTTCCGGCATCCTGTTCGTGCTGCTGATCGGCCTGGTATGGTTCGCCAAGCCGCCG
TTCACCGCTTCCGGCGGCGACAGCGGCGCGCACTGA

Upstream 100 bases:

>100_bases
AGAAATCCTGGCCGCCAACGCCGGCAAGTAAGGACGCCCACCCATGAACCATCCGCCACTGAGCGGCTCGAAACTGGTTT
GGATCACGCTGGCGTTGTCG

Downstream 100 bases:

>100_bases
GCAAGATCCAAGCCTCGGCACAAACGGGCCCTGCTGAACAGCGGGGCCTGTTTTTTTTGACACCGCACCCCGCTCTCGCC
TCTTTCCTTCCGGCGCTCCC

Product: multidrug resistance protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 491; Mature: 490

Protein sequence:

>491_residues
MSVFMQVLDTTIANVALPTISGNLGAATSQGTWVITSFGVANAIAVPLTGWLAKRFGEVKLFTASTLLFVLTSWLCGMSG
SLEMLIFFRVLQGAVAGPMIPLSQSLLLSCYPAAKKSMALALWAMTVIVAPVFGPILGGVISDNWHWGWIFFINVPVGLA
AAFISWRILKSRETDTFDLPIDKLGLALLVIGVGSLQMVLDRGKELDWFNSAEIVTLAVIAFLALTYLVIWELGEEHPIV
DLSLFSQRNFTVGTVAISLGFMLYFGAVVLLPLMLQTQLGYTATWAGLAAAPIGLIPVVLSPIIGKNAHRWDMRWIVTVS
FSVYAICFFWRSTFNTDMDFSYVVWPQVVQGIGVACFFMPLTTITLSGLHPSRIASASSLSNFLRILAGSIGTSITTTMW
DRREAQHHSKLTEHVTQYDPASVSWFDTMGKLGLGPSQQHALTQLQITKQGYIMAANEIFWVSGILFVLLIGLVWFAKPP
FTASGGDSGAH

Sequences:

>Translated_491_residues
MSVFMQVLDTTIANVALPTISGNLGAATSQGTWVITSFGVANAIAVPLTGWLAKRFGEVKLFTASTLLFVLTSWLCGMSG
SLEMLIFFRVLQGAVAGPMIPLSQSLLLSCYPAAKKSMALALWAMTVIVAPVFGPILGGVISDNWHWGWIFFINVPVGLA
AAFISWRILKSRETDTFDLPIDKLGLALLVIGVGSLQMVLDRGKELDWFNSAEIVTLAVIAFLALTYLVIWELGEEHPIV
DLSLFSQRNFTVGTVAISLGFMLYFGAVVLLPLMLQTQLGYTATWAGLAAAPIGLIPVVLSPIIGKNAHRWDMRWIVTVS
FSVYAICFFWRSTFNTDMDFSYVVWPQVVQGIGVACFFMPLTTITLSGLHPSRIASASSLSNFLRILAGSIGTSITTTMW
DRREAQHHSKLTEHVTQYDPASVSWFDTMGKLGLGPSQQHALTQLQITKQGYIMAANEIFWVSGILFVLLIGLVWFAKPP
FTASGGDSGAH
>Mature_490_residues
SVFMQVLDTTIANVALPTISGNLGAATSQGTWVITSFGVANAIAVPLTGWLAKRFGEVKLFTASTLLFVLTSWLCGMSGS
LEMLIFFRVLQGAVAGPMIPLSQSLLLSCYPAAKKSMALALWAMTVIVAPVFGPILGGVISDNWHWGWIFFINVPVGLAA
AFISWRILKSRETDTFDLPIDKLGLALLVIGVGSLQMVLDRGKELDWFNSAEIVTLAVIAFLALTYLVIWELGEEHPIVD
LSLFSQRNFTVGTVAISLGFMLYFGAVVLLPLMLQTQLGYTATWAGLAAAPIGLIPVVLSPIIGKNAHRWDMRWIVTVSF
SVYAICFFWRSTFNTDMDFSYVVWPQVVQGIGVACFFMPLTTITLSGLHPSRIASASSLSNFLRILAGSIGTSITTTMWD
RREAQHHSKLTEHVTQYDPASVSWFDTMGKLGLGPSQQHALTQLQITKQGYIMAANEIFWVSGILFVLLIGLVWFAKPPF
TASGGDSGAH

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=482, Percent_Identity=65.3526970954357, Blast_Score=643, Evalue=0.0,
Organism=Escherichia coli, GI1788710, Length=486, Percent_Identity=54.9382716049383, Blast_Score=538, Evalue=1e-154,
Organism=Escherichia coli, GI1790195, Length=420, Percent_Identity=29.7619047619048, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI87081983, Length=405, Percent_Identity=26.4197530864197, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1788392, Length=417, Percent_Identity=29.7362110311751, Blast_Score=127, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6322958, Length=158, Percent_Identity=26.5822784810127, Blast_Score=64, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 53456; Mature: 53325

Theoretical pI: Translated: 7.28; Mature: 7.28

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVFMQVLDTTIANVALPTISGNLGAATSQGTWVITSFGVANAIAVPLTGWLAKRFGEVK
CHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCEE
LFTASTLLFVLTSWLCGMSGSLEMLIFFRVLQGAVAGPMIPLSQSLLLSCYPAAKKSMAL
EEHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCHHHHHHHHH
ALWAMTVIVAPVFGPILGGVISDNWHWGWIFFINVPVGLAAAFISWRILKSRETDTFDLP
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCHHHHHHHHHHHHHHCCCCCCCCCC
IDKLGLALLVIGVGSLQMVLDRGKELDWFNSAEIVTLAVIAFLALTYLVIWELGEEHPIV
HHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEE
DLSLFSQRNFTVGTVAISLGFMLYFGAVVLLPLMLQTQLGYTATWAGLAAAPIGLIPVVL
EEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
SPIIGKNAHRWDMRWIVTVSFSVYAICFFWRSTFNTDMDFSYVVWPQVVQGIGVACFFMP
HHHHCCCCCEECEEEEEEEHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHH
LTTITLSGLHPSRIASASSLSNFLRILAGSIGTSITTTMWDRREAQHHSKLTEHVTQYDP
HHHHEECCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
ASVSWFDTMGKLGLGPSQQHALTQLQITKQGYIMAANEIFWVSGILFVLLIGLVWFAKPP
CCCHHHHHHHCCCCCCCHHHHHHHEEEECCCEEEEECHHHHHHHHHHHHHHHHHHHCCCC
FTASGGDSGAH
CCCCCCCCCCC
>Mature Secondary Structure 
SVFMQVLDTTIANVALPTISGNLGAATSQGTWVITSFGVANAIAVPLTGWLAKRFGEVK
HHHHHHHHHHHHHHEECCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCEE
LFTASTLLFVLTSWLCGMSGSLEMLIFFRVLQGAVAGPMIPLSQSLLLSCYPAAKKSMAL
EEHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCHHHHHHHHH
ALWAMTVIVAPVFGPILGGVISDNWHWGWIFFINVPVGLAAAFISWRILKSRETDTFDLP
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCHHHHHHHHHHHHHHCCCCCCCCCC
IDKLGLALLVIGVGSLQMVLDRGKELDWFNSAEIVTLAVIAFLALTYLVIWELGEEHPIV
HHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEE
DLSLFSQRNFTVGTVAISLGFMLYFGAVVLLPLMLQTQLGYTATWAGLAAAPIGLIPVVL
EEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
SPIIGKNAHRWDMRWIVTVSFSVYAICFFWRSTFNTDMDFSYVVWPQVVQGIGVACFFMP
HHHHCCCCCEECEEEEEEEHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHH
LTTITLSGLHPSRIASASSLSNFLRILAGSIGTSITTTMWDRREAQHHSKLTEHVTQYDP
HHHHEECCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
ASVSWFDTMGKLGLGPSQQHALTQLQITKQGYIMAANEIFWVSGILFVLLIGLVWFAKPP
CCCHHHHHHHCCCCCCCHHHHHHHEEEECCCEEEEECHHHHHHHHHHHHHHHHHHHCCCC
FTASGGDSGAH
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]