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The map label for this gene is emrB [H]

Identifier: 34496163

GI number: 34496163

Start: 733478

End: 735022

Strand: Reverse

Name: emrB [H]

Synonym: CV_0708

Alternate gene names: 34496163

Gene position: 735022-733478 (Counterclockwise)

Preceding gene: 34496164

Following gene: 34496162

Centisome position: 15.47

GC content: 68.8

Gene sequence:

>1545_bases
ATGTCTAGCGCGAATCCGGCGGCCGGCGAAATCCAGCGGCCGCTGAGCGGCAATATGCTGTGGCTGGCGGCGCTGGTGCT
GGCCGCCGCCAACTTCATGGTGGTGCTGGACACCACCATCGCCAACGTGTCGGTGCCCAATATCGCCGGCGGGCTGGCGG
TATCGTCCAGCCAGGGCACTTATGTCATCACCTCCTACGCGGTGGCCGAGGCCATCATCGTGCCGCTGACCGGCTGGCTG
GCGGCGCGCTTCGGCACCGTGCGGCTGTTCGTCGGCTCGATGCTGCTGTTCGGCCTGTTCTCCATGCTGTGCGGCCTGGC
CGGCTCGCTGGAGACGCTGGTGCTGTTCCGCGTGCTGCAGGGCCTGGCCGGCGGCCCGCTGATGCCGATGTCGCAGACGC
TGCTGCTGCAGATCTTCCCCAGGCAGAAGGCCGGCGCGGCGATAGGGCTGTGGAGCATGACCACGCTGGTGGCGCCCATC
ATGGGCCCCATCCTGGGCGGCACGCTGTGCGACCAGGCCAGCTGGCCGTGGATTTTCTACATCAACGTGCCCATCGCGCT
GGGTTGCGGCTTGCTCGGCTGGAGGCTGCTCAAGCGCTACCAGAGCCAAACCGCCAAACTGCGGATAGACGCGGCGGGCC
TGGCGCTGCTGGTGCTGTGGGTGGGCGCGCTGCAGCTGATGCTGGACAAGGGCAAGGAGCTGGATTGGTTCGGTTCGCCC
CTCATCGTCGGCCTGGCCATCGCCGCGGTGATCGGCTTCGCCGCCTTCCTGATCTGGGAGCTGACCGAGGCGCAGCCCAT
CGTCGATCTCAGAGTGTTCCGCCACCGCGGCTTCTGGGCCAGCGTGCTGACCATCTCGCTGGCGTTCGGATCGTTCTTCG
GCGCCACCGTGCTGACGCCGCAATGGCTGCAGGCCTATATGGGCTACACCGCCACCCAGGCCGGCTACGCCACTGCGATG
AGCGGCGTGCTGGCGGTGATGGCGGCGCCGATCGCCGCCCAGCTGTCCTCGCGGCTGGACCCGCGCAAGCTGGTGTTCTG
CGGCGTGATGTGGCTGGGCTGCGTCACGCTGTACCGCAGCTTCTCCACCACCGACATGGGCTTCTGGCAGGTGGCGCTGC
CGCTGCTGTTCCAGGGCTTGGGCATGCCCTTCTTCTTCGTGCCGCTGACCGGGCTGGCGCTGGCTAGCGTGGAGCCGGCG
GAAGTGGCCTCGGCCGCCGGCCTGATGAGCTTCATGCGCACCTTGTCCGGCGCCTTCGCCACATCCTTGGTCACCACCGC
CTGGGACGACAAGGCCAGCTACAACCACGCCGAGCTGGCCGGCATGGTGGACGGCGGCGGCGACGCGCTGCGCGCGCTGA
GCGCGGGCGGCCTGTCCGACGGCGCGGCCTGGGGCGTGATCGAACAAACGCTGCAGGCGCAGAGCGTCATGCTGTCCACC
AACCAGATCTTCTGGCTGGCCGGCCTGTCTTTCGTCGTCGCCGCCTGCGCCATCTGGCTGGCGCCCCGTCCCAGCCGCGT
CGCCGACACCAGCCAGGCCCACTGA

Upstream 100 bases:

>100_bases
CCGGTGCGCATCGCGCTGGACCAGGCGGAGCTGAAAGCCCATCCGCTGCAGGTGGGGCTGTCGATGGAAGCCACGATAGA
CGTCAGCGAGTGAGGCCATC

Downstream 100 bases:

>100_bases
GTTTCTCGACCCGCGTTTCACCACTTGCCCCGCGCCCTTGTGGCCGGGGCTTTTTTTGCCCTGTCTCGATGGCCAGTCCT
TACCTGCGGCCACCATTCGG

Product: multidrug resistance protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 514; Mature: 513

Protein sequence:

>514_residues
MSSANPAAGEIQRPLSGNMLWLAALVLAAANFMVVLDTTIANVSVPNIAGGLAVSSSQGTYVITSYAVAEAIIVPLTGWL
AARFGTVRLFVGSMLLFGLFSMLCGLAGSLETLVLFRVLQGLAGGPLMPMSQTLLLQIFPRQKAGAAIGLWSMTTLVAPI
MGPILGGTLCDQASWPWIFYINVPIALGCGLLGWRLLKRYQSQTAKLRIDAAGLALLVLWVGALQLMLDKGKELDWFGSP
LIVGLAIAAVIGFAAFLIWELTEAQPIVDLRVFRHRGFWASVLTISLAFGSFFGATVLTPQWLQAYMGYTATQAGYATAM
SGVLAVMAAPIAAQLSSRLDPRKLVFCGVMWLGCVTLYRSFSTTDMGFWQVALPLLFQGLGMPFFFVPLTGLALASVEPA
EVASAAGLMSFMRTLSGAFATSLVTTAWDDKASYNHAELAGMVDGGGDALRALSAGGLSDGAAWGVIEQTLQAQSVMLST
NQIFWLAGLSFVVAACAIWLAPRPSRVADTSQAH

Sequences:

>Translated_514_residues
MSSANPAAGEIQRPLSGNMLWLAALVLAAANFMVVLDTTIANVSVPNIAGGLAVSSSQGTYVITSYAVAEAIIVPLTGWL
AARFGTVRLFVGSMLLFGLFSMLCGLAGSLETLVLFRVLQGLAGGPLMPMSQTLLLQIFPRQKAGAAIGLWSMTTLVAPI
MGPILGGTLCDQASWPWIFYINVPIALGCGLLGWRLLKRYQSQTAKLRIDAAGLALLVLWVGALQLMLDKGKELDWFGSP
LIVGLAIAAVIGFAAFLIWELTEAQPIVDLRVFRHRGFWASVLTISLAFGSFFGATVLTPQWLQAYMGYTATQAGYATAM
SGVLAVMAAPIAAQLSSRLDPRKLVFCGVMWLGCVTLYRSFSTTDMGFWQVALPLLFQGLGMPFFFVPLTGLALASVEPA
EVASAAGLMSFMRTLSGAFATSLVTTAWDDKASYNHAELAGMVDGGGDALRALSAGGLSDGAAWGVIEQTLQAQSVMLST
NQIFWLAGLSFVVAACAIWLAPRPSRVADTSQAH
>Mature_513_residues
SSANPAAGEIQRPLSGNMLWLAALVLAAANFMVVLDTTIANVSVPNIAGGLAVSSSQGTYVITSYAVAEAIIVPLTGWLA
ARFGTVRLFVGSMLLFGLFSMLCGLAGSLETLVLFRVLQGLAGGPLMPMSQTLLLQIFPRQKAGAAIGLWSMTTLVAPIM
GPILGGTLCDQASWPWIFYINVPIALGCGLLGWRLLKRYQSQTAKLRIDAAGLALLVLWVGALQLMLDKGKELDWFGSPL
IVGLAIAAVIGFAAFLIWELTEAQPIVDLRVFRHRGFWASVLTISLAFGSFFGATVLTPQWLQAYMGYTATQAGYATAMS
GVLAVMAAPIAAQLSSRLDPRKLVFCGVMWLGCVTLYRSFSTTDMGFWQVALPLLFQGLGMPFFFVPLTGLALASVEPAE
VASAAGLMSFMRTLSGAFATSLVTTAWDDKASYNHAELAGMVDGGGDALRALSAGGLSDGAAWGVIEQTLQAQSVMLSTN
QIFWLAGLSFVVAACAIWLAPRPSRVADTSQAH

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=499, Percent_Identity=44.8897795591182, Blast_Score=405, Evalue=1e-114,
Organism=Escherichia coli, GI1788710, Length=499, Percent_Identity=41.0821643286573, Blast_Score=368, Evalue=1e-103,
Organism=Escherichia coli, GI1790195, Length=416, Percent_Identity=31.0096153846154, Blast_Score=172, Evalue=4e-44,
Organism=Escherichia coli, GI1788392, Length=403, Percent_Identity=33.0024813895782, Blast_Score=140, Evalue=3e-34,
Organism=Escherichia coli, GI87081983, Length=411, Percent_Identity=27.7372262773723, Blast_Score=120, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6325455, Length=124, Percent_Identity=30.6451612903226, Blast_Score=67, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6322958, Length=179, Percent_Identity=28.4916201117318, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 54367; Mature: 54235

Theoretical pI: Translated: 7.78; Mature: 7.78

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSANPAAGEIQRPLSGNMLWLAALVLAAANFMVVLDTTIANVSVPNIAGGLAVSSSQGT
CCCCCCCCHHHCCCCCCCHHHHHHHHHHHCCEEEEEECHHHCCCCCCCCCCCEEECCCCC
YVITSYAVAEAIIVPLTGWLAARFGTVRLFVGSMLLFGLFSMLCGLAGSLETLVLFRVLQ
EEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
GLAGGPLMPMSQTLLLQIFPRQKAGAAIGLWSMTTLVAPIMGPILGGTLCDQASWPWIFY
HCCCCCCCCCHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHHCCHHHCCCCCCEEEE
INVPIALGCGLLGWRLLKRYQSQTAKLRIDAAGLALLVLWVGALQLMLDKGKELDWFGSP
ECCHHHHHHHHHHHHHHHHHHCCHHEEEECHHHHHHHHHHHHHHHHHHHCCCCCCCCCCH
LIVGLAIAAVIGFAAFLIWELTEAQPIVDLRVFRHRGFWASVLTISLAFGSFFGATVLTP
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCH
QWLQAYMGYTATQAGYATAMSGVLAVMAAPIAAQLSSRLDPRKLVFCGVMWLGCVTLYRS
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHC
FSTTDMGFWQVALPLLFQGLGMPFFFVPLTGLALASVEPAEVASAAGLMSFMRTLSGAFA
CCCCCCHHHHHHHHHHHHHCCCCEEHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
TSLVTTAWDDKASYNHAELAGMVDGGGDALRALSAGGLSDGAAWGVIEQTLQAQSVMLST
HHHHHHCCCCCCCCCHHHHHEEECCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCC
NQIFWLAGLSFVVAACAIWLAPRPSRVADTSQAH
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
SSANPAAGEIQRPLSGNMLWLAALVLAAANFMVVLDTTIANVSVPNIAGGLAVSSSQGT
CCCCCCCHHHCCCCCCCHHHHHHHHHHHCCEEEEEECHHHCCCCCCCCCCCEEECCCCC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CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]