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The map label for this gene is emrK [H]

Identifier: 34496164

GI number: 34496164

Start: 735030

End: 736172

Strand: Reverse

Name: emrK [H]

Synonym: CV_0709

Alternate gene names: 34496164

Gene position: 736172-735030 (Counterclockwise)

Preceding gene: 34496165

Following gene: 34496163

Centisome position: 15.49

GC content: 71.04

Gene sequence:

>1143_bases
ATGAGCGAAAACGCACAAGCCCTGCCGGCCGCCGCGGCGCCCGCCGCCGCCGGCCGTCCCCTGCAACGCAAGAAGCTGTT
CACGCTGCTGTTCGGCGCCGTCGCCGCCGCGGCCGTCGCCTACGGCGGCTACTGGACGCTGATCGCCTCCCATCGCGTCA
GCACCGACAACGCCTATGTCGCCGCCGAGATCGCCCAGGTCACGCCCGAGGTGGCCGGCACCGTCAAGGAAGTGAAGGTG
GTCGACACCCAGCCGGTGAAGGCCGGCGAGGTGCTGGTGGTGCTGAACGACAGCGACGCCAGGCTGGCGCTGGCCCAGGC
CGAGGCCGACCTCGCCCGCGCCGAGCGCCGGGTGAAGGGCTACTTCGCCAACGACGAGGGCCTGTCGGCCCAAGCCCAGG
CGCGCAGCGCCGACCAGGCCCGCTCCGCCGCGCAGCTGGGATCGGCCCGCGCCGACCTGGCGCGCGCCAGGCTCGACCTG
CAGCGCCGCCAAGCGCTGGCAAAGAGCGGCTCGGTGTCCGGCGAGGAGCTGAGCAACGCGCAGAACGCCTACTCCACCGC
GCAGGCCAGCCTGAAGGCCGCCGAGGCCGCCAGCCGCCAGAGCGAGGCCAACTACCGCGCCGCCGTCGGCAGCCTGAAGG
CCAACCAGACGCTGACCGCAAACACCACGGTGGACGACAACCCCGAAGTGACGCTGGCGCGCGCCAAGCGCGACCAGGCC
AGGCTGGACCTGGAACGCACGGTGATCCGCGCCCAGGTGGACGGCGTGGTGGCCAAGCGCCAGGCCCAGGTGGGCCAGCG
CGTGCAGTCCGGCACGCCGCTGATGTCGGTGGTGCCGCTGCAGAACGCCCACGTCGACGCCAACTTCAAGGAAGTGCAGT
TGAACAAGGTACGCGTCGGCCAGCCGGTGGAGCTGACCGCCGACCTCTACGGCAACAAGGTGGTGTACCACGGCAAGGTG
GCCGGCCTGTCCGGCGGCTCTGGTTCGGCCTTCGCGATGATTCCGGCGCAGAACGCCACCGGCAACTGGATCAAGGTGGT
GCAGCGGCTGCCGGTGCGCATCGCGCTGGACCAGGCGGAGCTGAAAGCCCATCCGCTGCAGGTGGGGCTGTCGATGGAAG
CCACGATAGACGTCAGCGAGTGA

Upstream 100 bases:

>100_bases
TCGCTGGATGTGGCGCTTGTCAAGGCGCTGGGCGGCGGCTACCAGGCAGCCGAACCCGCCCGCTGAGGCCGCCGGCAAAC
CCATACCAGGACAATCCATC

Downstream 100 bases:

>100_bases
GGCCATCATGTCTAGCGCGAATCCGGCGGCCGGCGAAATCCAGCGGCCGCTGAGCGGCAATATGCTGTGGCTGGCGGCGC
TGGTGCTGGCCGCCGCCAAC

Product: multidrug resistance transmembrane protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 380; Mature: 379

Protein sequence:

>380_residues
MSENAQALPAAAAPAAAGRPLQRKKLFTLLFGAVAAAAVAYGGYWTLIASHRVSTDNAYVAAEIAQVTPEVAGTVKEVKV
VDTQPVKAGEVLVVLNDSDARLALAQAEADLARAERRVKGYFANDEGLSAQAQARSADQARSAAQLGSARADLARARLDL
QRRQALAKSGSVSGEELSNAQNAYSTAQASLKAAEAASRQSEANYRAAVGSLKANQTLTANTTVDDNPEVTLARAKRDQA
RLDLERTVIRAQVDGVVAKRQAQVGQRVQSGTPLMSVVPLQNAHVDANFKEVQLNKVRVGQPVELTADLYGNKVVYHGKV
AGLSGGSGSAFAMIPAQNATGNWIKVVQRLPVRIALDQAELKAHPLQVGLSMEATIDVSE

Sequences:

>Translated_380_residues
MSENAQALPAAAAPAAAGRPLQRKKLFTLLFGAVAAAAVAYGGYWTLIASHRVSTDNAYVAAEIAQVTPEVAGTVKEVKV
VDTQPVKAGEVLVVLNDSDARLALAQAEADLARAERRVKGYFANDEGLSAQAQARSADQARSAAQLGSARADLARARLDL
QRRQALAKSGSVSGEELSNAQNAYSTAQASLKAAEAASRQSEANYRAAVGSLKANQTLTANTTVDDNPEVTLARAKRDQA
RLDLERTVIRAQVDGVVAKRQAQVGQRVQSGTPLMSVVPLQNAHVDANFKEVQLNKVRVGQPVELTADLYGNKVVYHGKV
AGLSGGSGSAFAMIPAQNATGNWIKVVQRLPVRIALDQAELKAHPLQVGLSMEATIDVSE
>Mature_379_residues
SENAQALPAAAAPAAAGRPLQRKKLFTLLFGAVAAAAVAYGGYWTLIASHRVSTDNAYVAAEIAQVTPEVAGTVKEVKVV
DTQPVKAGEVLVVLNDSDARLALAQAEADLARAERRVKGYFANDEGLSAQAQARSADQARSAAQLGSARADLARARLDLQ
RRQALAKSGSVSGEELSNAQNAYSTAQASLKAAEAASRQSEANYRAAVGSLKANQTLTANTTVDDNPEVTLARAKRDQAR
LDLERTVIRAQVDGVVAKRQAQVGQRVQSGTPLMSVVPLQNAHVDANFKEVQLNKVRVGQPVELTADLYGNKVVYHGKVA
GLSGGSGSAFAMIPAQNATGNWIKVVQRLPVRIALDQAELKAHPLQVGLSMEATIDVSE

Specific function: Unknown

COG id: COG1566

COG function: function code V; Multidrug resistance efflux pump

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788711, Length=355, Percent_Identity=39.4366197183099, Blast_Score=255, Evalue=4e-69,
Organism=Escherichia coli, GI1789041, Length=358, Percent_Identity=40.7821229050279, Blast_Score=249, Evalue=3e-67,
Organism=Escherichia coli, GI1790519, Length=314, Percent_Identity=30.5732484076433, Blast_Score=112, Evalue=6e-26,
Organism=Escherichia coli, GI87081951, Length=335, Percent_Identity=25.6716417910448, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1789637, Length=138, Percent_Identity=34.7826086956522, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005694
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 40004; Mature: 39873

Theoretical pI: Translated: 9.90; Mature: 9.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSENAQALPAAAAPAAAGRPLQRKKLFTLLFGAVAAAAVAYGGYWTLIASHRVSTDNAYV
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECEECCCCCEE
AAEIAQVTPEVAGTVKEVKVVDTQPVKAGEVLVVLNDSDARLALAQAEADLARAERRVKG
EHHHHHHCHHHHCCHHEEEEECCCCCCCCCEEEEEECCCCEEEEHHHHHHHHHHHHHHHC
YFANDEGLSAQAQARSADQARSAAQLGSARADLARARLDLQRRQALAKSGSVSGEELSNA
EEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
QNAYSTAQASLKAAEAASRQSEANYRAAVGSLKANQTLTANTTVDDNPEVTLARAKRDQA
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCEEEECCCCCCCCCEEEEECCCHHH
RLDLERTVIRAQVDGVVAKRQAQVGQRVQSGTPLMSVVPLQNAHVDANFKEVQLNKVRVG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCEEEEEEEECC
QPVELTADLYGNKVVYHGKVAGLSGGSGSAFAMIPAQNATGNWIKVVQRLPVRIALDQAE
CCCEEEHCCCCCEEEEEEEEEECCCCCCCEEEEEECCCCCCHHHHHHHHCCEEEEECHHH
LKAHPLQVGLSMEATIDVSE
HHCCCEEECCEEEEEEEECC
>Mature Secondary Structure 
SENAQALPAAAAPAAAGRPLQRKKLFTLLFGAVAAAAVAYGGYWTLIASHRVSTDNAYV
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECEECCCCCEE
AAEIAQVTPEVAGTVKEVKVVDTQPVKAGEVLVVLNDSDARLALAQAEADLARAERRVKG
EHHHHHHCHHHHCCHHEEEEECCCCCCCCCEEEEEECCCCEEEEHHHHHHHHHHHHHHHC
YFANDEGLSAQAQARSADQARSAAQLGSARADLARARLDLQRRQALAKSGSVSGEELSNA
EEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
QNAYSTAQASLKAAEAASRQSEANYRAAVGSLKANQTLTANTTVDDNPEVTLARAKRDQA
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCEEEECCCCCCCCCEEEEECCCHHH
RLDLERTVIRAQVDGVVAKRQAQVGQRVQSGTPLMSVVPLQNAHVDANFKEVQLNKVRVG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCEEEEEEEECC
QPVELTADLYGNKVVYHGKVAGLSGGSGSAFAMIPAQNATGNWIKVVQRLPVRIALDQAE
CCCEEEHCCCCCEEEEEEEEEECCCCCCCEEEEEECCCCCCHHHHHHHHCCEEEEECHHH
LKAHPLQVGLSMEATIDVSE
HHCCCEEECCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]