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The map label for this gene is ispB [H]

Identifier: 339905956

GI number: 339905956

Start: 510457

End: 511455

Strand: Reverse

Name: ispB [H]

Synonym: NA

Alternate gene names: 339905956

Gene position: 511455-510457 (Counterclockwise)

Preceding gene: 238026157

Following gene: 238026151

Centisome position: 13.09

GC content: 64.56

Gene sequence:

>999_bases
ATGATCATGTCGTCCACTGCCTCCCCTTCCCTTAGCGCCGCGCAACTGTTGGCTCCGATCGCCGACGATATGGAGCAGAT
CAACCGCGTCATCCGGCAGAGCCTGTCGTCCGACGTGCTGCTGATCAACCAGATTGCCGAATACATCATCGGCGCGGGCG
GCAAGCGACTGCGGCCGGCGTTGCTGCTGCTCGTCGCGGGCGCGCTCGGTGACGGCTCGAGCCACCGGCACACGCTGGCC
GCCGTCGTCGAGTTCATTCACACGGCCACGCTGCTGCACGACGACGTGGTCGACGAATCGGAGTTGCGGCGCGGCCGCAA
GACCGCCAACGCGCTGTTCGGCAACGCGGCCAGCGTGCTGGTCGGCGATTACCTCTACTCGCGCTCGTTCGAGATGATGG
TGGGCGTCGGCAAGATGCGCGTGATGGAGATCCTCTCGGAAGCCACCACGATCATCTCCGAGGGCGAGGTGCTGCAACTG
CTGAACATGCATGACGCCGACGTCGACGAGTCGCGCTACATGCAGGTGATCCGCTACAAGACGGCCAAGCTGTTCGAGGC
GTCGGCGCGCCTGGGCGCGGTGCTGGCCGGGGCCGACGCGAGCACCGAGGCCGCCGCCGCCGAATACGGCCGCCGCATCG
GCACCGCGTTCCAGATCATGGACGACTGGCTGGACTACGCTGGTACCGCCGAATCGATGGGCAAGAACGCCGGCGACGAC
CTGCGCGAAGGCAAGCCGACGCTGCCGCTGATCCATCTGCTCGAACACGGCACGCCGGAGCAGCAGACGCTCGCACGCGA
GGCCATCGAGCAGGGCGGCACCGATCGCTTCGATACGATCTTCGACGCGATCACGCGCTCGGGCGCGCTCGATCACACGC
TGGAATGCGCGCGTCAGGAAGCGCAGGCGGCGGCCGATGCAATTTTTTCTTTCCCCTCTTCCATTTTCAAAAACTCGCTG
CTAGAATTATGTTCTTACTCGACGACACGAAAGTCTTGA

Upstream 100 bases:
NA

Downstream 100 bases:
NA

Product: Octaprenyl-diphosphate synthase

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:
NA

Sequences:

>Translated_332_residues
MIMSSTASPSLSAAQLLAPIADDMEQINRVIRQSLSSDVLLINQIAEYIIGAGGKRLRPALLLLVAGALGDGSSHRHTLA
AVVEFIHTATLLHDDVVDESELRRGRKTANALFGNAASVLVGDYLYSRSFEMMVGVGKMRVMEILSEATTIISEGEVLQL
LNMHDADVDESRYMQVIRYKTAKLFEASARLGAVLAGADASTEAAAAEYGRRIGTAFQIMDDWLDYAGTAESMGKNAGDD
LREGKPTLPLIHLLEHGTPEQQTLAREAIEQGGTDRFDTIFDAITRSGALDHTLECARQEAQAAADAIFSFPSSIFKNSL
LELCSYSTTRKS
>Mature_332_residues
MIMSSTASPSLSAAQLLAPIADDMEQINRVIRQSLSSDVLLINQIAEYIIGAGGKRLRPALLLLVAGALGDGSSHRHTLA
AVVEFIHTATLLHDDVVDESELRRGRKTANALFGNAASVLVGDYLYSRSFEMMVGVGKMRVMEILSEATTIISEGEVLQL
LNMHDADVDESRYMQVIRYKTAKLFEASARLGAVLAGADASTEAAAAEYGRRIGTAFQIMDDWLDYAGTAESMGKNAGDD
LREGKPTLPLIHLLEHGTPEQQTLAREAIEQGGTDRFDTIFDAITRSGALDHTLECARQEAQAAADAIFSFPSSIFKNSL
LELCSYSTTRKS

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=315, Percent_Identity=28.5714285714286, Blast_Score=134, Evalue=9e-32,
Organism=Homo sapiens, GI4758430, Length=207, Percent_Identity=24.1545893719807, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI83700220, Length=207, Percent_Identity=24.1545893719807, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1789578, Length=312, Percent_Identity=51.2820512820513, Blast_Score=329, Evalue=1e-91,
Organism=Escherichia coli, GI1786623, Length=243, Percent_Identity=32.0987654320988, Blast_Score=92, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17505681, Length=303, Percent_Identity=33.003300330033, Blast_Score=147, Evalue=6e-36,
Organism=Saccharomyces cerevisiae, GI6319475, Length=242, Percent_Identity=31.404958677686, Blast_Score=120, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6325188, Length=234, Percent_Identity=24.7863247863248, Blast_Score=86, Evalue=7e-18,
Organism=Drosophila melanogaster, GI24651612, Length=271, Percent_Identity=31.7343173431734, Blast_Score=138, Evalue=6e-33,
Organism=Drosophila melanogaster, GI281365769, Length=260, Percent_Identity=25.7692307692308, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24660002, Length=260, Percent_Identity=25.7692307692308, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 35899; Mature: 35899

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIMSSTASPSLSAAQLLAPIADDMEQINRVIRQSLSSDVLLINQIAEYIIGAGGKRLRPA
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHH
LLLLVAGALGDGSSHRHTLAAVVEFIHTATLLHDDVVDESELRRGRKTANALFGNAASVL
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHH
VGDYLYSRSFEMMVGVGKMRVMEILSEATTIISEGEVLQLLNMHDADVDESRYMQVIRYK
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCHHHHHHHHHHH
TAKLFEASARLGAVLAGADASTEAAAAEYGRRIGTAFQIMDDWLDYAGTAESMGKNAGDD
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCH
LREGKPTLPLIHLLEHGTPEQQTLAREAIEQGGTDRFDTIFDAITRSGALDHTLECARQE
HHCCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHH
AQAAADAIFSFPSSIFKNSLLELCSYSTTRKS
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MIMSSTASPSLSAAQLLAPIADDMEQINRVIRQSLSSDVLLINQIAEYIIGAGGKRLRPA
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHH
LLLLVAGALGDGSSHRHTLAAVVEFIHTATLLHDDVVDESELRRGRKTANALFGNAASVL
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHH
VGDYLYSRSFEMMVGVGKMRVMEILSEATTIISEGEVLQLLNMHDADVDESRYMQVIRYK
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCHHHHHHHHHHH
TAKLFEASARLGAVLAGADASTEAAAAEYGRRIGTAFQIMDDWLDYAGTAESMGKNAGDD
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCH
LREGKPTLPLIHLLEHGTPEQQTLAREAIEQGGTDRFDTIFDAITRSGALDHTLECARQE
HHCCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHH
AQAAADAIFSFPSSIFKNSLLELCSYSTTRKS
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8312607; 9278503; 2670911; 8037730 [H]