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The map label for this gene is speB [C]

Identifier: 33864478

GI number: 33864478

Start: 2340269

End: 2341183

Strand: Direct

Name: speB [C]

Synonym: PMT2214

Alternate gene names: 33864478

Gene position: 2340269-2341183 (Clockwise)

Preceding gene: 161350039

Following gene: 33864479

Centisome position: 97.07

GC content: 57.05

Gene sequence:

>915_bases
ATGCCCCAAAACACAACAACCACTGCAACAAGATTCGACAGCGAAGGTGCCATCTTCATGGCTGCAAGACGCGACCCTAC
CGGATGCAACGTCGGACTTTTTGGCGTGCCTTACGACGGCACCTCTTCATTCCGCCCAGGCAGCCGCTTCGGTCCGACAG
CCATCCGCGACGTCAGCAATGGCCTAGAAACCTACTGCCCTCAGCTTGATCTGGATCTAGAAGATCTTGCCTTCACTGAC
CTTGGCGCCCTTGAAATTCCCTGCGGCGACCCCAAGCCCGTTGTAGAAGCAGTCAAAAAAGCCACCCAAAACGTGCTGAA
GATGGGTCTCAGACCGCTGATGCTAGGCGGCGAGCACTCGATCAGCTCCGGAGCAGTGGCTGCAATCACTGAATTGCATT
CAGATCTCGTGCTGTTGCAACTGGATGCCCATGCAGACCTCCGCCAAGAGTATCTAGGTGCGCGACACAGCCATGCCTGT
GCCATGCGCCGTTGCCTCGAGGTCCTGCCAAGCGCTCAGCTGCTGCAATTCGCAATCCGCAGTGGCACACGCGCAGAATT
TCAAGAACTACACGATCAGCAACGGCTGGTGAACCACAACCCCGGCCAAGCAGCGACTGAGCTCGCTAAAGCCCTTGCAC
CCCATCAAGGCAAACCTCTCTATCTCACCGTGGATTTGGATTGGTTCGACCCATCCGTACTGCCGGGGACGGGCACACCT
GAACCAGGCGGCTTTCTATGGCGCGATTTTGCCGTCGTCGTGGATGTACTACGACACCATCAACTGGTAGGCGCTGACGT
GGTGGAACTCGCCCCCCAACTTGATCCTTCAGGGATGAGTAGTGTGCTCGGTGCCAAGGTGACCCGCAGCCTGCTCATGC
TGCTCAGCCTGTCTACAAGATCTGAGCAAAAGTGA

Upstream 100 bases:

>100_bases
GAGCAGCCGCCATCTCCGCAGGCTGTGAAATCTGGAGCCCACGCTGGCAACAGGGTGCCTTTGAAGCCATCCCAGCTTTT
ATTGAAAGGGAGCTGACTTG

Downstream 100 bases:

>100_bases
GCAGCATCACTCCATTGCTTCAGCAGAGTTTCACGCTAGATCTTCGAACACTCAAAGCAGCTCGAAAAACATCCAGGTCT
GAAAACAGCTCCTGTATCCC

Product: arginase

Products: putrescine; urea

Alternate protein names: NA

Number of amino acids: Translated: 304; Mature: 303

Protein sequence:

>304_residues
MPQNTTTTATRFDSEGAIFMAARRDPTGCNVGLFGVPYDGTSSFRPGSRFGPTAIRDVSNGLETYCPQLDLDLEDLAFTD
LGALEIPCGDPKPVVEAVKKATQNVLKMGLRPLMLGGEHSISSGAVAAITELHSDLVLLQLDAHADLRQEYLGARHSHAC
AMRRCLEVLPSAQLLQFAIRSGTRAEFQELHDQQRLVNHNPGQAATELAKALAPHQGKPLYLTVDLDWFDPSVLPGTGTP
EPGGFLWRDFAVVVDVLRHHQLVGADVVELAPQLDPSGMSSVLGAKVTRSLLMLLSLSTRSEQK

Sequences:

>Translated_304_residues
MPQNTTTTATRFDSEGAIFMAARRDPTGCNVGLFGVPYDGTSSFRPGSRFGPTAIRDVSNGLETYCPQLDLDLEDLAFTD
LGALEIPCGDPKPVVEAVKKATQNVLKMGLRPLMLGGEHSISSGAVAAITELHSDLVLLQLDAHADLRQEYLGARHSHAC
AMRRCLEVLPSAQLLQFAIRSGTRAEFQELHDQQRLVNHNPGQAATELAKALAPHQGKPLYLTVDLDWFDPSVLPGTGTP
EPGGFLWRDFAVVVDVLRHHQLVGADVVELAPQLDPSGMSSVLGAKVTRSLLMLLSLSTRSEQK
>Mature_303_residues
PQNTTTTATRFDSEGAIFMAARRDPTGCNVGLFGVPYDGTSSFRPGSRFGPTAIRDVSNGLETYCPQLDLDLEDLAFTDL
GALEIPCGDPKPVVEAVKKATQNVLKMGLRPLMLGGEHSISSGAVAAITELHSDLVLLQLDAHADLRQEYLGARHSHACA
MRRCLEVLPSAQLLQFAIRSGTRAEFQELHDQQRLVNHNPGQAATELAKALAPHQGKPLYLTVDLDWFDPSVLPGTGTPE
PGGFLWRDFAVVVDVLRHHQLVGADVVELAPQLDPSGMSSVLGAKVTRSLLMLLSLSTRSEQK

Specific function: Catalyzes The Formation Of Putrescine From Agmatine. [C]

COG id: COG0010

COG function: function code E; Arginase/agmatinase/formimionoglutamate hydrolase, arginase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the arginase family [H]

Homologues:

Organism=Homo sapiens, GI37537722, Length=284, Percent_Identity=31.3380281690141, Blast_Score=128, Evalue=6e-30,
Organism=Escherichia coli, GI1789306, Length=251, Percent_Identity=31.8725099601594, Blast_Score=96, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005925
- InterPro:   IPR006035
- InterPro:   IPR020855 [H]

Pfam domain/function: PF00491 Arginase [H]

EC number: 3.5.3.11

Molecular weight: Translated: 32855; Mature: 32724

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: PS00147 ARGINASE_1 ; PS00148 ARGINASE_2 ; PS01053 ARGINASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQNTTTTATRFDSEGAIFMAARRDPTGCNVGLFGVPYDGTSSFRPGSRFGPTAIRDVSN
CCCCCCCCCEEECCCCCEEEEECCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHH
GLETYCPQLDLDLEDLAFTDLGALEIPCGDPKPVVEAVKKATQNVLKMGLRPLMLGGEHS
HHHHHCCCCCCCHHHHHHCCCCCEECCCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCC
ISSGAVAAITELHSDLVLLQLDAHADLRQEYLGARHSHACAMRRCLEVLPSAQLLQFAIR
CCCCHHHHHHHHHCCEEEEEECCCHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHH
SGTRAEFQELHDQQRLVNHNPGQAATELAKALAPHQGKPLYLTVDLDWFDPSVLPGTGTP
CCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCC
EPGGFLWRDFAVVVDVLRHHQLVGADVVELAPQLDPSGMSSVLGAKVTRSLLMLLSLSTR
CCCCHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
SEQK
CCCC
>Mature Secondary Structure 
PQNTTTTATRFDSEGAIFMAARRDPTGCNVGLFGVPYDGTSSFRPGSRFGPTAIRDVSN
CCCCCCCCEEECCCCCEEEEECCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHH
GLETYCPQLDLDLEDLAFTDLGALEIPCGDPKPVVEAVKKATQNVLKMGLRPLMLGGEHS
HHHHHCCCCCCCHHHHHHCCCCCEECCCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCC
ISSGAVAAITELHSDLVLLQLDAHADLRQEYLGARHSHACAMRRCLEVLPSAQLLQFAIR
CCCCHHHHHHHHHCCEEEEEECCCHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHH
SGTRAEFQELHDQQRLVNHNPGQAATELAKALAPHQGKPLYLTVDLDWFDPSVLPGTGTP
CCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCC
EPGGFLWRDFAVVVDVLRHHQLVGADVVELAPQLDPSGMSSVLGAKVTRSLLMLLSLSTR
CCCCHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
SEQK
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: agmatine; H2O

Specific reaction: agmatine + H2O = putrescine + urea

General reaction: Amidine hydrolysis [C]

Inhibitor: EDTA; EGTA [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]