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The map label for this gene is speE [H]

Identifier: 161350039

GI number: 161350039

Start: 2339409

End: 2340269

Strand: Direct

Name: speE [H]

Synonym: PMT2213

Alternate gene names: 161350039

Gene position: 2339409-2340269 (Clockwise)

Preceding gene: 33864476

Following gene: 33864478

Centisome position: 97.04

GC content: 56.56

Gene sequence:

>861_bases
ATGGACAGCCCTTCAACAACAGCAAATGGCTGGATCGATGAACACCACCAGGGTGTTCGCTATGGCCTGCAAGGCCGTGT
GCTGGTTGATGAAACAAGCCCATACCAGCGAATCACGGTGATCGATAGCAGCCGCTACGGAAAAGGGCTGTTGCTCGACG
GCTGCTGGATGACCGCAGAACACCAGGAACGCCACTATCACGAATCCTTGGTCCACCCAGCTCTGTGTAGCGCGGCCCAA
CTTGAACGGGTGTTGGTGATTGGGGGCGGCGATGGCGGCACAGCCAGGGAGTGCCTGCGCTACCAAGACGTCAAACACCT
CGACATGGTGGAAATTGATCGCCGAGTCGTGGAACTGAGCCAAAAGCATCTGCCCAGCCTTGGAAGCCATGCTTGGAGCG
ATCCGCGCTTACAGCTCAACCTGGAAAACGGCATTGCCTGGGTAGCCAATGCAACTGAATCCTCCTACGACGTGATTCTT
ATCGACGGATCAGATCCTGCAGGGCCTGCAGAAGGTCTCTTCAATCAAACCTTCTTTGAGCATTGCCGACGCATCCTGCG
GCCCGGAGGAGTTTTTGCTACTCAAAGCGAGTCACCAGAAGCATTCCGTCAGGTTCACATCGACACTGTTCGCCTGATCC
GTCAAGTTTTCGGCTACGCAGATCCCCTCTACGGCTGGGTGCCGATGTATCCAAGCGGCTGGTGGAGCTGGACCTTCGCC
GCCATCGATGGTCCTCGTTATCGCAACCCACTCCCAGCAAGAGCAGCCGCCATCTCCGCAGGCTGTGAAATCTGGAGCCC
ACGCTGGCAACAGGGTGCCTTTGAAGCCATCCCAGCTTTTATTGAAAGGGAGCTGACTTGA

Upstream 100 bases:

>100_bases
GCAAGCGATTCGTAATCAAAAGGTCTTGCACCATAGACCCACGCTGGCATCAAAGCCCTATGGAGGGCTTAGCTGGAAGA
AAGTTTTAGGCCTCCTTGCC

Downstream 100 bases:

>100_bases
TGCCCCAAAACACAACAACCACTGCAACAAGATTCGACAGCGAAGGTGCCATCTTCATGGCTGCAAGACGCGACCCTACC
GGATGCAACGTCGGACTTTT

Product: spermidine synthase

Products: NA

Alternate protein names: Putrescine aminopropyltransferase; PAPT; SPDSY [H]

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MDSPSTTANGWIDEHHQGVRYGLQGRVLVDETSPYQRITVIDSSRYGKGLLLDGCWMTAEHQERHYHESLVHPALCSAAQ
LERVLVIGGGDGGTARECLRYQDVKHLDMVEIDRRVVELSQKHLPSLGSHAWSDPRLQLNLENGIAWVANATESSYDVIL
IDGSDPAGPAEGLFNQTFFEHCRRILRPGGVFATQSESPEAFRQVHIDTVRLIRQVFGYADPLYGWVPMYPSGWWSWTFA
AIDGPRYRNPLPARAAAISAGCEIWSPRWQQGAFEAIPAFIERELT

Sequences:

>Translated_286_residues
MDSPSTTANGWIDEHHQGVRYGLQGRVLVDETSPYQRITVIDSSRYGKGLLLDGCWMTAEHQERHYHESLVHPALCSAAQ
LERVLVIGGGDGGTARECLRYQDVKHLDMVEIDRRVVELSQKHLPSLGSHAWSDPRLQLNLENGIAWVANATESSYDVIL
IDGSDPAGPAEGLFNQTFFEHCRRILRPGGVFATQSESPEAFRQVHIDTVRLIRQVFGYADPLYGWVPMYPSGWWSWTFA
AIDGPRYRNPLPARAAAISAGCEIWSPRWQQGAFEAIPAFIERELT
>Mature_286_residues
MDSPSTTANGWIDEHHQGVRYGLQGRVLVDETSPYQRITVIDSSRYGKGLLLDGCWMTAEHQERHYHESLVHPALCSAAQ
LERVLVIGGGDGGTARECLRYQDVKHLDMVEIDRRVVELSQKHLPSLGSHAWSDPRLQLNLENGIAWVANATESSYDVIL
IDGSDPAGPAEGLFNQTFFEHCRRILRPGGVFATQSESPEAFRQVHIDTVRLIRQVFGYADPLYGWVPMYPSGWWSWTFA
AIDGPRYRNPLPARAAAISAGCEIWSPRWQQGAFEAIPAFIERELT

Specific function: Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor [H]

COG id: COG0421

COG function: function code E; Spermidine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the spermidine/spermine synthase family [H]

Homologues:

Organism=Homo sapiens, GI63253298, Length=246, Percent_Identity=32.520325203252, Blast_Score=132, Evalue=4e-31,
Organism=Escherichia coli, GI1786312, Length=274, Percent_Identity=32.4817518248175, Blast_Score=161, Evalue=5e-41,
Organism=Caenorhabditis elegans, GI212645616, Length=227, Percent_Identity=37.0044052863436, Blast_Score=131, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI25154106, Length=273, Percent_Identity=34.4322344322344, Blast_Score=128, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6323175, Length=215, Percent_Identity=38.1395348837209, Blast_Score=144, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6325326, Length=227, Percent_Identity=33.0396475770925, Blast_Score=135, Evalue=8e-33,
Organism=Drosophila melanogaster, GI24645443, Length=291, Percent_Identity=28.5223367697594, Blast_Score=131, Evalue=6e-31,
Organism=Drosophila melanogaster, GI21355941, Length=212, Percent_Identity=28.3018867924528, Blast_Score=104, Evalue=7e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001045 [H]

Pfam domain/function: PF01564 Spermine_synth [H]

EC number: =2.5.1.16 [H]

Molecular weight: Translated: 32099; Mature: 32099

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: PS51006 SPERMIDINE_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSPSTTANGWIDEHHQGVRYGLQGRVLVDETSPYQRITVIDSSRYGKGLLLDGCWMTAE
CCCCCCCCCCCHHHHCCCHHCCCCCCEEEECCCCCEEEEEEECCCCCCEEEEECEEECCH
HQERHYHESLVHPALCSAAQLERVLVIGGGDGGTARECLRYQDVKHLDMVEIDRRVVELS
HHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHH
QKHLPSLGSHAWSDPRLQLNLENGIAWVANATESSYDVILIDGSDPAGPAEGLFNQTFFE
HHHCCCCCCCCCCCCCEEEEECCCEEEEEECCCCCEEEEEEECCCCCCHHHHHHHHHHHH
HCRRILRPGGVFATQSESPEAFRQVHIDTVRLIRQVFGYADPLYGWVPMYPSGWWSWTFA
HHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCEEEEE
AIDGPRYRNPLPARAAAISAGCEIWSPRWQQGAFEAIPAFIERELT
EECCCCCCCCCCHHHHHHHCCCCCCCCCHHCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MDSPSTTANGWIDEHHQGVRYGLQGRVLVDETSPYQRITVIDSSRYGKGLLLDGCWMTAE
CCCCCCCCCCCHHHHCCCHHCCCCCCEEEECCCCCEEEEEEECCCCCCEEEEECEEECCH
HQERHYHESLVHPALCSAAQLERVLVIGGGDGGTARECLRYQDVKHLDMVEIDRRVVELS
HHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHH
QKHLPSLGSHAWSDPRLQLNLENGIAWVANATESSYDVILIDGSDPAGPAEGLFNQTFFE
HHHCCCCCCCCCCCCCEEEEECCCEEEEEECCCCCEEEEEEECCCCCCHHHHHHHHHHHH
HCRRILRPGGVFATQSESPEAFRQVHIDTVRLIRQVFGYADPLYGWVPMYPSGWWSWTFA
HHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCEEEEE
AIDGPRYRNPLPARAAAISAGCEIWSPRWQQGAFEAIPAFIERELT
EECCCCCCCCCCHHHHHHHCCCCCCCCCHHCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA