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The map label for this gene is glcE [H]

Identifier: 33864204

GI number: 33864204

Start: 2048829

End: 2050055

Strand: Direct

Name: glcE [H]

Synonym: PMT1939

Alternate gene names: 33864204

Gene position: 2048829-2050055 (Clockwise)

Preceding gene: 33864202

Following gene: 33864205

Centisome position: 84.98

GC content: 58.19

Gene sequence:

>1227_bases
GTGAAAACGTTCTTCGCCGATACCGCCAACGTGCCAGCACCTGCTACGGCAAAGGAGCTGGCCGCTCTTGTTGCAGAGCT
GCATGCAGCGGCAACCCCCTGGCTGCCCTGTGGTCTTGGCAGCCGTCTGCAATGGGGGCCGCCTGTGGAGATACCTTCAC
AACCAATCAGTGTGAGTGGTCTCAAGCGCATCCTTGATCACGCTGTCGACGACCTCACGATCACTGTTGAAGCAGGCCTG
CCGCTGGCAGAGCTACAAATGGTTTTGAAAGAACACCATCAATGGCTAGCCGTCAACTGGCCCAGGGGAAGCCAAGCCAA
CACAGATCCCAACAGTGCTGGCAGTGTGGGCGGGTTGGTGGCTCGTGGCCTCGCCGGCGGTTTGCGACAGCGGCATCTTG
GTATCCGCGACCAACTGATCGGAATCGGACTCCTGCGTAGCGATGGCATTGCTGCGCATGCTGGAGGCCGGGTAGTCAAA
AACGTGGCTGGCTATGACCTCATGCGCCTGCTGTGCGGCAGTTGGGGCAGCTTGGCTCTGATCACGGAACTCACTCTGCG
CTGCCAACCAATCCGCCCAGCACACATCGGCCTTCGCCTTGATGGTTCTCTGGAAGCGCTGGAAGCATGGCGAGCTGCAC
TATTGCGCACCAGCTTCACCCCGGAATACTGCGATTGGATCAAAACAGATTTAGCAAACTGGCATCTCAACATCGGTGTG
GCCAGCGTGAGCGATCAAGCCGTCAACGACCAAATAGGCCATCTCAAAGCCCTCGCCAATCAACATCAACTGGGCACAAA
ACAGCTCGACTGGCTCGGCCCCCATCAAGAGCCACACCCATCTGAAGGATCCTTCGATAGCAACCATTGGCTGGTACGGG
TAGCGCTGCAACCAAGCAACATTCACCGGCTGATCGCCAGCAAAGAACTGCAAGCTCTCAACGGCTGGCATTGGCAGCTT
GCCGCTGGCGCTGGCATCGGCGACGGCTGGCAAATATCAGCAGACAACGCCAAAGGGATCAGCCAAAGCGAGGATCTCAA
AGCGCTGCGCCTCGAGATTGCTCGCCTCGGCGGCCAGCTCACGCTCCTGCAGCAACCCAGCCAAGCTGGCGATCACCTGC
CTGCTTGGCTGGATGCACCATCAAGGCCACTGATTGAAGCTGTGAAGCAACAATTCGATCCAAAGCAACAGCTAGCGAGA
GGCCGATTGCCAGGAGTTGCTGGATAA

Upstream 100 bases:

>100_bases
ACATCAACATCCCAGCGCAGCAGCAGCAGACAGCCATGCACAGTCTGGGAGCCGGTGGGGTCGTCATCAAGCACCACAAT
CTTCATGGCACCATCGTGAC

Downstream 100 bases:

>100_bases
CAAATAACGAAAGGCAATTATACTAAGGTGCGAAAGCCGAGGATGATTATTAGATGCAATAATTGCATCTAATAAATGCA
GCACTTAATCATTCACTTCA

Product: glycolate oxidase subunit GlcE

Products: 2-oxo acid; H2O2

Alternate protein names: NA

Number of amino acids: Translated: 408; Mature: 408

Protein sequence:

>408_residues
MKTFFADTANVPAPATAKELAALVAELHAAATPWLPCGLGSRLQWGPPVEIPSQPISVSGLKRILDHAVDDLTITVEAGL
PLAELQMVLKEHHQWLAVNWPRGSQANTDPNSAGSVGGLVARGLAGGLRQRHLGIRDQLIGIGLLRSDGIAAHAGGRVVK
NVAGYDLMRLLCGSWGSLALITELTLRCQPIRPAHIGLRLDGSLEALEAWRAALLRTSFTPEYCDWIKTDLANWHLNIGV
ASVSDQAVNDQIGHLKALANQHQLGTKQLDWLGPHQEPHPSEGSFDSNHWLVRVALQPSNIHRLIASKELQALNGWHWQL
AAGAGIGDGWQISADNAKGISQSEDLKALRLEIARLGGQLTLLQQPSQAGDHLPAWLDAPSRPLIEAVKQQFDPKQQLAR
GRLPGVAG

Sequences:

>Translated_408_residues
MKTFFADTANVPAPATAKELAALVAELHAAATPWLPCGLGSRLQWGPPVEIPSQPISVSGLKRILDHAVDDLTITVEAGL
PLAELQMVLKEHHQWLAVNWPRGSQANTDPNSAGSVGGLVARGLAGGLRQRHLGIRDQLIGIGLLRSDGIAAHAGGRVVK
NVAGYDLMRLLCGSWGSLALITELTLRCQPIRPAHIGLRLDGSLEALEAWRAALLRTSFTPEYCDWIKTDLANWHLNIGV
ASVSDQAVNDQIGHLKALANQHQLGTKQLDWLGPHQEPHPSEGSFDSNHWLVRVALQPSNIHRLIASKELQALNGWHWQL
AAGAGIGDGWQISADNAKGISQSEDLKALRLEIARLGGQLTLLQQPSQAGDHLPAWLDAPSRPLIEAVKQQFDPKQQLAR
GRLPGVAG
>Mature_408_residues
MKTFFADTANVPAPATAKELAALVAELHAAATPWLPCGLGSRLQWGPPVEIPSQPISVSGLKRILDHAVDDLTITVEAGL
PLAELQMVLKEHHQWLAVNWPRGSQANTDPNSAGSVGGLVARGLAGGLRQRHLGIRDQLIGIGLLRSDGIAAHAGGRVVK
NVAGYDLMRLLCGSWGSLALITELTLRCQPIRPAHIGLRLDGSLEALEAWRAALLRTSFTPEYCDWIKTDLANWHLNIGV
ASVSDQAVNDQIGHLKALANQHQLGTKQLDWLGPHQEPHPSEGSFDSNHWLVRVALQPSNIHRLIASKELQALNGWHWQL
AAGAGIGDGWQISADNAKGISQSEDLKALRLEIARLGGQLTLLQQPSQAGDHLPAWLDAPSRPLIEAVKQQFDPKQQLAR
GRLPGVAG

Specific function: Unknown

COG id: COG0277

COG function: function code C; FAD/FMN-containing dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain [H]

Homologues:

Organism=Homo sapiens, GI37595756, Length=280, Percent_Identity=25.7142857142857, Blast_Score=78, Evalue=2e-14,
Organism=Escherichia coli, GI48994914, Length=174, Percent_Identity=33.9080459770115, Blast_Score=83, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6320027, Length=284, Percent_Identity=24.2957746478873, Blast_Score=80, Evalue=8e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016166
- InterPro:   IPR016164
- InterPro:   IPR016168
- InterPro:   IPR006094
- InterPro:   IPR016171 [H]

Pfam domain/function: PF01565 FAD_binding_4 [H]

EC number: 1.1.3.15

Molecular weight: Translated: 43924; Mature: 43924

Theoretical pI: Translated: 7.28; Mature: 7.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTFFADTANVPAPATAKELAALVAELHAAATPWLPCGLGSRLQWGPPVEIPSQPISVSG
CCCEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHH
LKRILDHAVDDLTITVEAGLPLAELQMVLKEHHQWLAVNWPRGSQANTDPNSAGSVGGLV
HHHHHHHHHCCEEEEEECCCCHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCHHHHH
ARGLAGGLRQRHLGIRDQLIGIGLLRSDGIAAHAGGRVVKNVAGYDLMRLLCGSWGSLAL
HHHHHHHHHHHHCCCHHHHHEEEEEECCCCEECCCCHHHHHHHHHHHHHHHHCCCCCHHH
ITELTLRCQPIRPAHIGLRLDGSLEALEAWRAALLRTSFTPEYCDWIKTDLANWHLNIGV
HHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCEEEEEEE
ASVSDQAVNDQIGHLKALANQHQLGTKQLDWLGPHQEPHPSEGSFDSNHWLVRVALQPSN
EECCCHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCCCCCCCEEEEEEECCCH
IHRLIASKELQALNGWHWQLAAGAGIGDGWQISADNAKGISQSEDLKALRLEIARLGGQL
HHHHHHHHHHHHHCCCEEEEEECCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCEE
TLLQQPSQAGDHLPAWLDAPSRPLIEAVKQQFDPKQQLARGRLPGVAG
EEEECCCCCCCCCCHHHCCCCCHHHHHHHHHCCHHHHHHCCCCCCCCC
>Mature Secondary Structure
MKTFFADTANVPAPATAKELAALVAELHAAATPWLPCGLGSRLQWGPPVEIPSQPISVSG
CCCEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHH
LKRILDHAVDDLTITVEAGLPLAELQMVLKEHHQWLAVNWPRGSQANTDPNSAGSVGGLV
HHHHHHHHHCCEEEEEECCCCHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCHHHHH
ARGLAGGLRQRHLGIRDQLIGIGLLRSDGIAAHAGGRVVKNVAGYDLMRLLCGSWGSLAL
HHHHHHHHHHHHCCCHHHHHEEEEEECCCCEECCCCHHHHHHHHHHHHHHHHCCCCCHHH
ITELTLRCQPIRPAHIGLRLDGSLEALEAWRAALLRTSFTPEYCDWIKTDLANWHLNIGV
HHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCEEEEEEE
ASVSDQAVNDQIGHLKALANQHQLGTKQLDWLGPHQEPHPSEGSFDSNHWLVRVALQPSN
EECCCHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCCCCCCCEEEEEEECCCH
IHRLIASKELQALNGWHWQLAAGAGIGDGWQISADNAKGISQSEDLKALRLEIARLGGQL
HHHHHHHHHHHHHCCCEEEEEECCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCEE
TLLQQPSQAGDHLPAWLDAPSRPLIEAVKQQFDPKQQLARGRLPGVAG
EEEECCCCCCCCCCHHHCCCCCHHHHHHHHHCCHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-2-hydroxy acid; O2

Specific reaction: (S)-2-hydroxy acid + O2 = 2-oxo acid + H2O2

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8606183; 9278503 [H]