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The map label for this gene is rffM [H]

Identifier: 33864131

GI number: 33864131

Start: 1970814

End: 1971557

Strand: Reverse

Name: rffM [H]

Synonym: PMT1864

Alternate gene names: 33864131

Gene position: 1971557-1970814 (Counterclockwise)

Preceding gene: 33864132

Following gene: 33864128

Centisome position: 81.78

GC content: 55.51

Gene sequence:

>744_bases
ATGGAATCAACAAGCACCGCACCGAGCGATCAATACCGTCGAAGAGTTCTAGGTGTGCCAGTGGATGCATGCCGCGATGT
TCAGGCTGCGGCGATTGGTTTGCATGCTCGTGGTGGTGGCCAGATCGTCACGCTTAATGCGGAGATGACAATGGCGGCTA
GAGAGAATCCAAGGCTAGGTGATGCTATTGCCGCGGCAGATCTAGTCATCCCAGATGGGATTGGCGTGGTTTGGGCGCTG
AGGCGTCAGGGAGTTGTGGTGCCGCGTAGTCCTGGCATTGAACTGGCACGTTCGTTACTTGCCTATGCAGAGGCTCATGA
TTGGAAGGTGGCCCTAGTGGGAGCAACCCCGCAGGTCATGGATCGTTTGAGGCAACTCCTGAAGCAAGACCTGCCTGCTC
TGCGACTTGTGATGTCTTCGCATGGTTATCAACCCCAAGATGCTTGGCCTGGCCTGGAAGCCAAGTTGAGAGTTTTGCAT
CCTGATCTTGTGCTTGTGGCTCTAGGAGTTCCCCGTCAAGAAACGTGGATAGCGCGCGTAAGGCGGGCATGTCCAGGCCT
CTGGATGGGTGTTGGGGGAAGTTTCGATGTATGGGCTGGGATGAAAAAGCGAGCCCCCGAATGGATGTGTCAGTTAGGGC
TCGAATGGCTCTATCGGTTGGGCCAAGAGCCCAGCCGATGGAATCGCATTCTTTGTCTGCCTACCTTCGTCTGGGAGGTA
TGGCGTCGAGGAAAAAAGCGTTGA

Upstream 100 bases:

>100_bases
CTGTTGCTCCATTCGCTTGCTTGTGGCGATTGGGCTTTGGACTCCATCGATTGCGTTCAAGGTTTAGAGCACTAAATTTT
CAACCTCAGTTGTCTCGCTG

Downstream 100 bases:

>100_bases
TTGAAATGAATGCTTCAGCGGAAGCCCACAGATGCTTGCCAAACAAAGACAAGCAGGAAGAAGAAAATGGGGATGAGTGG
AAGAACATCCACTGCTGGAG

Product: glycosyl transferase family protein

Products: NA

Alternate protein names: N-acetylglucosaminyldiphospho-undecaprenol N-acetyl-beta-D-mannosaminyltransferase; UDP-N-acetylmannosamine transferase [H]

Number of amino acids: Translated: 247; Mature: 247

Protein sequence:

>247_residues
MESTSTAPSDQYRRRVLGVPVDACRDVQAAAIGLHARGGGQIVTLNAEMTMAARENPRLGDAIAAADLVIPDGIGVVWAL
RRQGVVVPRSPGIELARSLLAYAEAHDWKVALVGATPQVMDRLRQLLKQDLPALRLVMSSHGYQPQDAWPGLEAKLRVLH
PDLVLVALGVPRQETWIARVRRACPGLWMGVGGSFDVWAGMKKRAPEWMCQLGLEWLYRLGQEPSRWNRILCLPTFVWEV
WRRGKKR

Sequences:

>Translated_247_residues
MESTSTAPSDQYRRRVLGVPVDACRDVQAAAIGLHARGGGQIVTLNAEMTMAARENPRLGDAIAAADLVIPDGIGVVWAL
RRQGVVVPRSPGIELARSLLAYAEAHDWKVALVGATPQVMDRLRQLLKQDLPALRLVMSSHGYQPQDAWPGLEAKLRVLH
PDLVLVALGVPRQETWIARVRRACPGLWMGVGGSFDVWAGMKKRAPEWMCQLGLEWLYRLGQEPSRWNRILCLPTFVWEV
WRRGKKR
>Mature_247_residues
MESTSTAPSDQYRRRVLGVPVDACRDVQAAAIGLHARGGGQIVTLNAEMTMAARENPRLGDAIAAADLVIPDGIGVVWAL
RRQGVVVPRSPGIELARSLLAYAEAHDWKVALVGATPQVMDRLRQLLKQDLPALRLVMSSHGYQPQDAWPGLEAKLRVLH
PDLVLVALGVPRQETWIARVRRACPGLWMGVGGSFDVWAGMKKRAPEWMCQLGLEWLYRLGQEPSRWNRILCLPTFVWEV
WRRGKKR

Specific function: Catalyzes the conversion of GlcNAc-PP-undecaprenol into ManNAc-GlcNAc-PP-undecaprenol, a key lipid intermediate in the de novo synthesis of teichoic acid (Potential) [H]

COG id: COG1922

COG function: function code M; Teichoic acid biosynthesis proteins

Gene ontology:

Cell location: Secreted, cell wall [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 26 family [H]

Homologues:

Organism=Escherichia coli, GI2367289, Length=232, Percent_Identity=34.9137931034483, Blast_Score=110, Evalue=7e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004629 [H]

Pfam domain/function: PF03808 Glyco_tran_WecB [H]

EC number: =2.4.1.187 [H]

Molecular weight: Translated: 27520; Mature: 27520

Theoretical pI: Translated: 10.43; Mature: 10.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESTSTAPSDQYRRRVLGVPVDACRDVQAAAIGLHARGGGQIVTLNAEMTMAARENPRLG
CCCCCCCCCHHHHHHHCCCCHHHHHCHHHHHEEEEECCCCEEEEEECCEEEECCCCCCCC
DAIAAADLVIPDGIGVVWALRRQGVVVPRSPGIELARSLLAYAEAHDWKVALVGATPQVM
HHHHHHHEECCCCCHHHHHHHHCCEEECCCCCHHHHHHHHHHHHCCCCEEEEECCCHHHH
DRLRQLLKQDLPALRLVMSSHGYQPQDAWPGLEAKLRVLHPDLVLVALGVPRQETWIARV
HHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCHHEEEEECCCEEEEEECCCCHHHHHHHH
RRACPGLWMGVGGSFDVWAGMKKRAPEWMCQLGLEWLYRLGQEPSRWNRILCLPTFVWEV
HHHCCCEEEECCCCCHHHCCHHHCCHHHHHHHHHHHHHHHCCCCCHHCEEEEHHHHHHHH
WRRGKKR
HHCCCCC
>Mature Secondary Structure
MESTSTAPSDQYRRRVLGVPVDACRDVQAAAIGLHARGGGQIVTLNAEMTMAARENPRLG
CCCCCCCCCHHHHHHHCCCCHHHHHCHHHHHEEEEECCCCEEEEEECCEEEECCCCCCCC
DAIAAADLVIPDGIGVVWALRRQGVVVPRSPGIELARSLLAYAEAHDWKVALVGATPQVM
HHHHHHHEECCCCCHHHHHHHHCCEEECCCCCHHHHHHHHHHHHCCCCEEEEECCCHHHH
DRLRQLLKQDLPALRLVMSSHGYQPQDAWPGLEAKLRVLHPDLVLVALGVPRQETWIARV
HHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCHHEEEEECCCEEEEEECCCCHHHHHHHH
RRACPGLWMGVGGSFDVWAGMKKRAPEWMCQLGLEWLYRLGQEPSRWNRILCLPTFVWEV
HHHCCCEEEECCCCCHHHCCHHHCCHHHHHHHHHHHHHHHCCCCCHHCEEEEHHHHHHHH
WRRGKKR
HHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11882717 [H]