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The map label for this gene is CobS [H]

Identifier: 33864128

GI number: 33864128

Start: 1968632

End: 1969414

Strand: Reverse

Name: CobS [H]

Synonym: PMT1861

Alternate gene names: 33864128

Gene position: 1969414-1968632 (Counterclockwise)

Preceding gene: 33864131

Following gene: 33864126

Centisome position: 81.69

GC content: 56.7

Gene sequence:

>783_bases
TTGCGCGCTCCCCTTTGGCTCCGGGATTTGGCTGGGGCATGGATCTTCTACTCCGTATTGCCTGCTTGGCCAGGGCTGAA
GCCACGTTTTGAACGGATTGCTCGCTTTGCTCCTTGGATCGGATTGGTGCTGGGAGGACTGCAGTCTTTCCTTTGGCTTG
TCTTGATCCGTGCCGATTGGCCGACCTCGGCAGTGACACTTTTGGTCATCGGGCTGGGTGCCTGGCTGTCGGGTGGTCTT
CACCTTGATGGGTTGATGGACACGGCCGATGGTCTGGCGGCTGGTCGTGAGCGCTGTCTGCAGGCTATGGACGACAGTTG
TGTTGGAGCTAGTGGAGTCCAGGCTCTGCTTGTGGTGGTGTTACTTCAGATTGCATCCCTACTTCGGCTTGGTTCTCTAG
CCCCTTTGGCTTTGCTGATTGCTGCCTTTTGGGGGCGTTGTGCGCCGCTTTGGGCCATGGCGCGCTTCTTTTACTTGCGT
GAGGGGCAGGCTGGCACCGCATCCTTTCATCGTCGTTATCGCAAGGGATGGCAGGAAGCATTACCAGCATGCTTAGTGCT
GCTTTTGGCATTAACGGTTGTCCCTTCACTCATGATCGTGGGGTGGCCCAGTCAGATGGTTTTGATGGCAGGAATTGGTG
TGGGTGTCTTGCCGGCGTTTTTAGTGCCTGAACTGCTTGGACGTCGTCTTGGTGGGCATAGCGGGGATTCTTATGGCGCT
TCAGTCGTGCTGGTGGAAACAATCACGCTTCTGTTGCTGGCGGTCTTACTGCCGGCAGGCTGA

Upstream 100 bases:

>100_bases
TCAAACACGGCTAGTGCTACGGCGATACTTCAGCGCCACAGGGCACCAGGCCGATCATGACCCTACGTTGGGCTTTGCCT
TTGGCTGGAGGTTGGAGATC

Downstream 100 bases:

>100_bases
GCAGAAAGGCATTCCCTTCGCTGGGAAGGGCTGGATCAAGGTTCGCTGGAGAGGTGATCAGTTGCAGCGAACCTCCCAAA
TCTTCAGCCAGTTGGCGTCC

Product: cobalamin-5-phosphate synthase CobS

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MRAPLWLRDLAGAWIFYSVLPAWPGLKPRFERIARFAPWIGLVLGGLQSFLWLVLIRADWPTSAVTLLVIGLGAWLSGGL
HLDGLMDTADGLAAGRERCLQAMDDSCVGASGVQALLVVVLLQIASLLRLGSLAPLALLIAAFWGRCAPLWAMARFFYLR
EGQAGTASFHRRYRKGWQEALPACLVLLLALTVVPSLMIVGWPSQMVLMAGIGVGVLPAFLVPELLGRRLGGHSGDSYGA
SVVLVETITLLLLAVLLPAG

Sequences:

>Translated_260_residues
MRAPLWLRDLAGAWIFYSVLPAWPGLKPRFERIARFAPWIGLVLGGLQSFLWLVLIRADWPTSAVTLLVIGLGAWLSGGL
HLDGLMDTADGLAAGRERCLQAMDDSCVGASGVQALLVVVLLQIASLLRLGSLAPLALLIAAFWGRCAPLWAMARFFYLR
EGQAGTASFHRRYRKGWQEALPACLVLLLALTVVPSLMIVGWPSQMVLMAGIGVGVLPAFLVPELLGRRLGGHSGDSYGA
SVVLVETITLLLLAVLLPAG
>Mature_260_residues
MRAPLWLRDLAGAWIFYSVLPAWPGLKPRFERIARFAPWIGLVLGGLQSFLWLVLIRADWPTSAVTLLVIGLGAWLSGGL
HLDGLMDTADGLAAGRERCLQAMDDSCVGASGVQALLVVVLLQIASLLRLGSLAPLALLIAAFWGRCAPLWAMARFFYLR
EGQAGTASFHRRYRKGWQEALPACLVLLLALTVVPSLMIVGWPSQMVLMAGIGVGVLPAFLVPELLGRRLGGHSGDSYGA
SVVLVETITLLLLAVLLPAG

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin) [H]

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family [H]

Homologues:

Organism=Escherichia coli, GI1788301, Length=238, Percent_Identity=35.2941176470588, Blast_Score=76, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003805 [H]

Pfam domain/function: PF02654 CobS [H]

EC number: 2.7.8.26

Molecular weight: Translated: 27887; Mature: 27887

Theoretical pI: Translated: 9.34; Mature: 9.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAPLWLRDLAGAWIFYSVLPAWPGLKPRFERIARFAPWIGLVLGGLQSFLWLVLIRADW
CCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PTSAVTLLVIGLGAWLSGGLHLDGLMDTADGLAAGRERCLQAMDDSCVGASGVQALLVVV
CHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
LLQIASLLRLGSLAPLALLIAAFWGRCAPLWAMARFFYLREGQAGTASFHRRYRKGWQEA
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
LPACLVLLLALTVVPSLMIVGWPSQMVLMAGIGVGVLPAFLVPELLGRRLGGHSGDSYGA
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCH
SVVLVETITLLLLAVLLPAG
HHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRAPLWLRDLAGAWIFYSVLPAWPGLKPRFERIARFAPWIGLVLGGLQSFLWLVLIRADW
CCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PTSAVTLLVIGLGAWLSGGLHLDGLMDTADGLAAGRERCLQAMDDSCVGASGVQALLVVV
CHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
LLQIASLLRLGSLAPLALLIAAFWGRCAPLWAMARFFYLREGQAGTASFHRRYRKGWQEA
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
LPACLVLLLALTVVPSLMIVGWPSQMVLMAGIGVGVLPAFLVPELLGRRLGGHSGDSYGA
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCH
SVVLVETITLLLLAVLLPAG
HHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA