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The map label for this gene is BirA [H]

Identifier: 33863626

GI number: 33863626

Start: 1445353

End: 1446135

Strand: Reverse

Name: BirA [H]

Synonym: PMT1359

Alternate gene names: 33863626

Gene position: 1446135-1445353 (Counterclockwise)

Preceding gene: 33863627

Following gene: 33863625

Centisome position: 59.98

GC content: 58.37

Gene sequence:

>783_bases
TTGCCCGGATGTGGCCTTGGTGGCATGCGCACATCTGACTCATGGACTGGTGCTGCTGCGGTTGCCCGTTTGTGGCGTAG
GCAGTCTTGCGGGGCTAGGCCATGGCAGTTGCGATGGCAGCCGGTCTGTGCGAGTACTGAGTTGATGTTGTCTGGTTGGC
TGAAGCAGCAGCCTTGTTCAGCTCAGCAGCCTCGGGCCTTGTTTGCTGATCGCCAGACCCATGGGAGGGGACAGCGTGGT
CGGGTCTGGCAGTCACCGATCGGTGGGGTTTGGATCAGTGCCGCTTTGCCCTGGTTTGATGCTCAGTGCTCTGCCGGTAT
TTTTGGAATAGCAGTGGCTGTGGCGCTGGCTGAAAGGCTCGAGCGGCGGGGAGTGCCCGTGAGTATCAAGTGGCCTAACG
ATTTGATGGTGGGGAACCGTAAATTGGCTGGTCTCTTGCCGAGATTGGTGCACCGTGGCAATCGCGTCAGGCTGGCACGC
ATTGGTCTTGGCCTGAATGTTTGCAACCGTGTGCCCAGAGAAGGGATTGCCTTAGATGAGTTGCTTCGATCAGGCCAGTG
TCAGCCTTTGGTCTGGATGGCAGAAGTGCTATGCGCTCTTGATCGAACTATGGATTTGGCTGGTCGAGCGGATTGGGTTT
GCGCCGAAGCTGAGCGACGCCTCTGGACAAAGCAGGTTCGTGATCCGAAGGGTGGTGAACTCTGGGACGTGATCGGACTG
GGACTGGATGGGTCGCTTTTGCTGAGCCAGGGTTCTCGGACCATGCGCTGGACACGTTGGTGA

Upstream 100 bases:

>100_bases
ACTGGAGTTGCCTTGACGCCTGGATCAGGTTTTGGCTCCGGCGGGGATGGTTGGCTTCGCCTGGCCCTGGTCCATCCGGT
GGAAGATTTGGAGGCGGCAG

Downstream 100 bases:

>100_bases
TAACTGAATTGGATTTTTCTATAGTTTTTCTCGTCTGAGGATCCGATCGGGTGCCGTTTCGCTGGTTCGCTGCTGCATGG
CTGCTTTCAGTGCCTGTCCT

Product: biotin--acetyl-CoA-carboxylase ligase

Products: NA

Alternate protein names: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase; Biotin--protein ligase [H]

Number of amino acids: Translated: 260; Mature: 259

Protein sequence:

>260_residues
MPGCGLGGMRTSDSWTGAAAVARLWRRQSCGARPWQLRWQPVCASTELMLSGWLKQQPCSAQQPRALFADRQTHGRGQRG
RVWQSPIGGVWISAALPWFDAQCSAGIFGIAVAVALAERLERRGVPVSIKWPNDLMVGNRKLAGLLPRLVHRGNRVRLAR
IGLGLNVCNRVPREGIALDELLRSGQCQPLVWMAEVLCALDRTMDLAGRADWVCAEAERRLWTKQVRDPKGGELWDVIGL
GLDGSLLLSQGSRTMRWTRW

Sequences:

>Translated_260_residues
MPGCGLGGMRTSDSWTGAAAVARLWRRQSCGARPWQLRWQPVCASTELMLSGWLKQQPCSAQQPRALFADRQTHGRGQRG
RVWQSPIGGVWISAALPWFDAQCSAGIFGIAVAVALAERLERRGVPVSIKWPNDLMVGNRKLAGLLPRLVHRGNRVRLAR
IGLGLNVCNRVPREGIALDELLRSGQCQPLVWMAEVLCALDRTMDLAGRADWVCAEAERRLWTKQVRDPKGGELWDVIGL
GLDGSLLLSQGSRTMRWTRW
>Mature_259_residues
PGCGLGGMRTSDSWTGAAAVARLWRRQSCGARPWQLRWQPVCASTELMLSGWLKQQPCSAQQPRALFADRQTHGRGQRGR
VWQSPIGGVWISAALPWFDAQCSAGIFGIAVAVALAERLERRGVPVSIKWPNDLMVGNRKLAGLLPRLVHRGNRVRLARI
GLGLNVCNRVPREGIALDELLRSGQCQPLVWMAEVLCALDRTMDLAGRADWVCAEAERRLWTKQVRDPKGGELWDVIGLG
LDGSLLLSQGSRTMRWTRW

Specific function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting protein

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Escherichia coli, GI1790408, Length=140, Percent_Identity=32.8571428571429, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004408
- InterPro:   IPR004409
- InterPro:   IPR003142
- InterPro:   IPR004143
- InterPro:   IPR008988 [H]

Pfam domain/function: PF02237 BPL_C; PF03099 BPL_LipA_LipB [H]

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 28881; Mature: 28750

Theoretical pI: Translated: 11.36; Mature: 11.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGCGLGGMRTSDSWTGAAAVARLWRRQSCGARPWQLRWQPVCASTELMLSGWLKQQPCS
CCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCC
AQQPRALFADRQTHGRGQRGRVWQSPIGGVWISAALPWFDAQCSAGIFGIAVAVALAERL
CCCCHHHHHCCHHCCCCCCCCCCCCCCCCEEHHHCCCCCCCCCCCCHHHHHHHHHHHHHH
ERRGVPVSIKWPNDLMVGNRKLAGLLPRLVHRGNRVRLARIGLGLNVCNRVPREGIALDE
HHCCCCEEEECCCCEEECCHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHCCCCCCCHHH
LLRSGQCQPLVWMAEVLCALDRTMDLAGRADWVCAEAERRLWTKQVRDPKGGELWDVIGL
HHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCEEHHEEC
GLDGSLLLSQGSRTMRWTRW
CCCCCEEECCCCCCEEECCC
>Mature Secondary Structure 
PGCGLGGMRTSDSWTGAAAVARLWRRQSCGARPWQLRWQPVCASTELMLSGWLKQQPCS
CCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCC
AQQPRALFADRQTHGRGQRGRVWQSPIGGVWISAALPWFDAQCSAGIFGIAVAVALAERL
CCCCHHHHHCCHHCCCCCCCCCCCCCCCCEEHHHCCCCCCCCCCCCHHHHHHHHHHHHHH
ERRGVPVSIKWPNDLMVGNRKLAGLLPRLVHRGNRVRLARIGLGLNVCNRVPREGIALDE
HHCCCCEEEECCCCEEECCHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHCCCCCCCHHH
LLRSGQCQPLVWMAEVLCALDRTMDLAGRADWVCAEAERRLWTKQVRDPKGGELWDVIGL
HHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCEEHHEEC
GLDGSLLLSQGSRTMRWTRW
CCCCCEEECCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]