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The map label for this gene is cysD [H]

Identifier: 33863147

GI number: 33863147

Start: 958391

End: 959719

Strand: Direct

Name: cysD [H]

Synonym: PMT0875

Alternate gene names: 33863147

Gene position: 958391-959719 (Clockwise)

Preceding gene: 33863146

Following gene: 33863148

Centisome position: 39.75

GC content: 50.56

Gene sequence:

>1329_bases
TTGACTTCACATCGCTTCGAAACCCTCCAGCTTCATGCCGGCCAGGTAGCTGATCCTGTCACGAACTCACGGGCTGTACC
CATTTATCAAACCAGTTCTTATGTCTTTAATGACGCTGAACATGGTGCCAACCTGTTTGGTCTTAAAGAATTCGGCAACA
TTTACACCCGTCTGATGAACCCCACGACGGATGTGTTTGAAAAGCGTGTTGCGGCTCTTGAGGGAGGGATTGCTGCTGTA
GCGACAGCTTCCGGACAATCCGCCCAGTTCCTGGCAATTACAAACTGCATGCAGGCTGGTGACAATCTTGTCTCAACATC
CTTCTTATATGGAGGAACCTACAATCAATTCAAGGTGCAGTTTCCCCGCCTTGGCATTGATGTGAAGTTTGCAGATGGTG
ATGATGTTGATAGCTTTGCCACACAAATCGATGCCAACACAAAAGCAATTTATGTGGAGTCGATGGGGAACCCTCGCTTC
AATATCCCTGACTTTAAAGGCCTTTCAGGCTTAGCAAAAGACATGGGAATCCCATTAATTGTTGACAACACCCTTGGAGC
AGCTGGTGCTTTGCTTCGGCCCATAGAGCATGGCGCGGATGTGGTGGTGGAAAGTGCCACCAAATGGATTGGAGGCCATG
GCACCAGCCTTGGTGGAGTGCTTGTGGATGCTGGCACCTTTAACTGGGGCAATGGCAAATTCCCACTGATGAGTGAGCCA
AGCGCGGCATATCACGGTCTGGTGCACTGGGATGCTTTCGGGTTCGGCAGCGACATCTGCTCCATGCTCGGCGTCCCAAG
CAATCGCAATGTGGCCTTTGCACTGCGAGCTCGAGTTGAGGGACTGCGTGACTGGGGAGCAGCTCTTAGTCCATTCAACT
CGTTCCTACTTTTGCAAGGGCTCGAAACACTCAGTTTGCGAGTGGAGCGCCATGCATCCAATGCCATGGCTCTTGCTACA
TGGCTGCAGGATCATCCCAAAGTTGCCAGTGTCAATTATCCCGGCCTGAAAAATGACCCATACCACGCACAAGCCAAAAC
ATACCTAACGAATCGAGGCATGGGATGCATGCTGATGTTCTCCCTTAAAGGAGGCTTTGATGATGCCGTGAGCTTCATCA
ATGGTCTTGAATTAGCTAGCCATCTCGCCAATGTTGGTGATGCCAAGACCTTGGTGATTCATCCTGCATCCACCACGCAT
CAACAACTTTCTGCTCAAGAGCAGGAATCTGCAGGAGTCACTCCCACAATGGTTAGGGTATCTGTTGGCCTTGAACACAT
CGAAGACATCAAGGCTGATTTTGAACAGGCCCTAGCGGCCATCAGTTGA

Upstream 100 bases:

>100_bases
CCATCCTTAGCAAAGACAGATAAAGCTGCTCCACCGGACGACTGGAACCGCGCCTGAGACTAAGTTGCTAGAAAGAAGGG
ACCTTCCTGTTTACGTGTAG

Downstream 100 bases:

>100_bases
GACTGGAGGCATCGATGGCGCTGATCCTGCCACGCAGTTATCACAAGATCGCCGCGGTGGAGAAGAACCGGATCTCATGG
ATCGAGCCGGAACTTGCGGA

Product: O-Acetyl homoserine sulfhydrylase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 442; Mature: 441

Protein sequence:

>442_residues
MTSHRFETLQLHAGQVADPVTNSRAVPIYQTSSYVFNDAEHGANLFGLKEFGNIYTRLMNPTTDVFEKRVAALEGGIAAV
ATASGQSAQFLAITNCMQAGDNLVSTSFLYGGTYNQFKVQFPRLGIDVKFADGDDVDSFATQIDANTKAIYVESMGNPRF
NIPDFKGLSGLAKDMGIPLIVDNTLGAAGALLRPIEHGADVVVESATKWIGGHGTSLGGVLVDAGTFNWGNGKFPLMSEP
SAAYHGLVHWDAFGFGSDICSMLGVPSNRNVAFALRARVEGLRDWGAALSPFNSFLLLQGLETLSLRVERHASNAMALAT
WLQDHPKVASVNYPGLKNDPYHAQAKTYLTNRGMGCMLMFSLKGGFDDAVSFINGLELASHLANVGDAKTLVIHPASTTH
QQLSAQEQESAGVTPTMVRVSVGLEHIEDIKADFEQALAAIS

Sequences:

>Translated_442_residues
MTSHRFETLQLHAGQVADPVTNSRAVPIYQTSSYVFNDAEHGANLFGLKEFGNIYTRLMNPTTDVFEKRVAALEGGIAAV
ATASGQSAQFLAITNCMQAGDNLVSTSFLYGGTYNQFKVQFPRLGIDVKFADGDDVDSFATQIDANTKAIYVESMGNPRF
NIPDFKGLSGLAKDMGIPLIVDNTLGAAGALLRPIEHGADVVVESATKWIGGHGTSLGGVLVDAGTFNWGNGKFPLMSEP
SAAYHGLVHWDAFGFGSDICSMLGVPSNRNVAFALRARVEGLRDWGAALSPFNSFLLLQGLETLSLRVERHASNAMALAT
WLQDHPKVASVNYPGLKNDPYHAQAKTYLTNRGMGCMLMFSLKGGFDDAVSFINGLELASHLANVGDAKTLVIHPASTTH
QQLSAQEQESAGVTPTMVRVSVGLEHIEDIKADFEQALAAIS
>Mature_441_residues
TSHRFETLQLHAGQVADPVTNSRAVPIYQTSSYVFNDAEHGANLFGLKEFGNIYTRLMNPTTDVFEKRVAALEGGIAAVA
TASGQSAQFLAITNCMQAGDNLVSTSFLYGGTYNQFKVQFPRLGIDVKFADGDDVDSFATQIDANTKAIYVESMGNPRFN
IPDFKGLSGLAKDMGIPLIVDNTLGAAGALLRPIEHGADVVVESATKWIGGHGTSLGGVLVDAGTFNWGNGKFPLMSEPS
AAYHGLVHWDAFGFGSDICSMLGVPSNRNVAFALRARVEGLRDWGAALSPFNSFLLLQGLETLSLRVERHASNAMALATW
LQDHPKVASVNYPGLKNDPYHAQAKTYLTNRGMGCMLMFSLKGGFDDAVSFINGLELASHLANVGDAKTLVIHPASTTHQ
QLSAQEQESAGVTPTMVRVSVGLEHIEDIKADFEQALAAIS

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=439, Percent_Identity=30.2961275626424, Blast_Score=165, Evalue=7e-41,
Organism=Homo sapiens, GI25453487, Length=438, Percent_Identity=27.6255707762557, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI299473759, Length=439, Percent_Identity=27.3348519362187, Blast_Score=129, Evalue=7e-30,
Organism=Escherichia coli, GI1790375, Length=415, Percent_Identity=30.6024096385542, Blast_Score=172, Evalue=5e-44,
Organism=Escherichia coli, GI1789383, Length=450, Percent_Identity=22, Blast_Score=103, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71980708, Length=456, Percent_Identity=29.3859649122807, Blast_Score=190, Evalue=1e-48,
Organism=Caenorhabditis elegans, GI17538055, Length=437, Percent_Identity=30.4347826086957, Blast_Score=167, Evalue=7e-42,
Organism=Caenorhabditis elegans, GI17560056, Length=388, Percent_Identity=30.4123711340206, Blast_Score=157, Evalue=1e-38,
Organism=Saccharomyces cerevisiae, GI6323333, Length=455, Percent_Identity=50.1098901098901, Blast_Score=413, Evalue=1e-116,
Organism=Saccharomyces cerevisiae, GI6319307, Length=443, Percent_Identity=31.1512415349887, Blast_Score=158, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6321254, Length=466, Percent_Identity=24.6781115879828, Blast_Score=102, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6321170, Length=350, Percent_Identity=26.5714285714286, Blast_Score=91, Evalue=3e-19,
Organism=Drosophila melanogaster, GI19922554, Length=439, Percent_Identity=28.9293849658314, Blast_Score=159, Evalue=5e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 47319; Mature: 47188

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSHRFETLQLHAGQVADPVTNSRAVPIYQTSSYVFNDAEHGANLFGLKEFGNIYTRLMN
CCCCCEEEEEECCCCCCCCCCCCCEEEEEECCCCEECCHHCCCCEEEHHHHHHHHHHHCC
PTTDVFEKRVAALEGGIAAVATASGQSAQFLAITNCMQAGDNLVSTSFLYGGTYNQFKVQ
CHHHHHHHHHHHHCCCEEEEEECCCCCCEEHHHHHHHHCCCCEEEEEEEECCCCCEEEEE
FPRLGIDVKFADGDDVDSFATQIDANTKAIYVESMGNPRFNIPDFKGLSGLAKDMGIPLI
ECEECEEEEECCCCCHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHCCCCEE
VDNTLGAAGALLRPIEHGADVVVESATKWIGGHGTSLGGVLVDAGTFNWGNGKFPLMSEP
EECCCCCHHHHHHHHHCCCHHEEHHHHHHHCCCCCCCCCEEEECCEEECCCCCCCCCCCC
SAAYHGLVHWDAFGFGSDICSMLGVPSNRNVAFALRARVEGLRDWGAALSPFNSFLLLQG
CHHHCCEEEEECCCCHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHC
LETLSLRVERHASNAMALATWLQDHPKVASVNYPGLKNDPYHAQAKTYLTNRGMGCMLMF
HHHHHHHHHHHCCCHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHHHHHCCCCCEEEEE
SLKGGFDDAVSFINGLELASHLANVGDAKTLVIHPASTTHQQLSAQEQESAGVTPTMVRV
EECCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCEEEEE
SVGLEHIEDIKADFEQALAAIS
HHCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TSHRFETLQLHAGQVADPVTNSRAVPIYQTSSYVFNDAEHGANLFGLKEFGNIYTRLMN
CCCCEEEEEECCCCCCCCCCCCCEEEEEECCCCEECCHHCCCCEEEHHHHHHHHHHHCC
PTTDVFEKRVAALEGGIAAVATASGQSAQFLAITNCMQAGDNLVSTSFLYGGTYNQFKVQ
CHHHHHHHHHHHHCCCEEEEEECCCCCCEEHHHHHHHHCCCCEEEEEEEECCCCCEEEEE
FPRLGIDVKFADGDDVDSFATQIDANTKAIYVESMGNPRFNIPDFKGLSGLAKDMGIPLI
ECEECEEEEECCCCCHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHCCCCEE
VDNTLGAAGALLRPIEHGADVVVESATKWIGGHGTSLGGVLVDAGTFNWGNGKFPLMSEP
EECCCCCHHHHHHHHHCCCHHEEHHHHHHHCCCCCCCCCEEEECCEEECCCCCCCCCCCC
SAAYHGLVHWDAFGFGSDICSMLGVPSNRNVAFALRARVEGLRDWGAALSPFNSFLLLQG
CHHHCCEEEEECCCCHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHC
LETLSLRVERHASNAMALATWLQDHPKVASVNYPGLKNDPYHAQAKTYLTNRGMGCMLMF
HHHHHHHHHHHCCCHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHHHHHCCCCCEEEEE
SLKGGFDDAVSFINGLELASHLANVGDAKTLVIHPASTTHQQLSAQEQESAGVTPTMVRV
EECCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCEEEEE
SVGLEHIEDIKADFEQALAAIS
HHCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]