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The map label for this gene is glyS [H]

Identifier: 33862851

GI number: 33862851

Start: 632476

End: 634641

Strand: Reverse

Name: glyS [H]

Synonym: PMT0578

Alternate gene names: 33862851

Gene position: 634641-632476 (Counterclockwise)

Preceding gene: 33862853

Following gene: 33862849

Centisome position: 26.32

GC content: 54.11

Gene sequence:

>2166_bases
GTGTCGACCCTTCTAGTGGAGATTGGGACTGAGGAGCTGCCAGCTGATTTTGCCCGGCTTGCCTTACCCCAACTGGAGCA
GATGGTAAGTCGTGACTTGCAAGGTCTACGTCTTAGCTACGGCATGCTCCAATGCACGAGTACGCCAAGGCGACTTGTCG
TGCTGGTTGAAAACTTGGCCAGCGCTACTGCTGATCTAGAAGAAGTACGCAAGGGTCCACCGGCTGGCCAAGCTTTCAAA
AATGGTGTGCCAACCCAGGCAGCCGCTGGCTTTGCCAAACGCTGTGGGCTGAAAGCAGAGGATCTCGAGATTCGGGAGAC
CCCGAAGGGACCTTTTGTGTTTGCAACCGTGCTTGAGCATGGGCGTTCTGCAATGGAACTACTAACAGACCAGATCCCTG
AGTGGATAGGTAGCCTTCAAGGCCGACGTTTCATGCGCTGGGGCGAAGGTGAAAGGCGTTTCTCTCGTCCGATTCGTTGG
CTTGTGGCCCTGCTCGATGAGCAGATCATCCCTGTTTGCCTTGACGGCAGCGACCCAGAGATTTGTGCAGCGAACCTGAG
TCGAGGCCATCGACTTCACGATCAAGCTGTCATCATTCCTTCTGCAGAAAGTTACGTACAAACCCTCGCCAAGGTTGGTG
TAGAGGTCGATCGGCAAAAACGTGGCACCTTGATCCGTCATGCAATTGATACGGCTGCTGCTGGTCTTGAAGCATGCCCT
AATTGTCCTGATGATCTTTTCGAAGAGCTCACCGACCTAGTGGAAGCACCTCTGCTTTTAGAGGGAACCTTCGCCAAGTC
ATATCTAGATCTACCCGCTGAAGTGCTCAGCACCGTAATGAGAGTGCATCAGCGCTATGTGCCCCTTTACCGTCTTGATG
CTGTCAATGACCCTCTTGCTCTCGATGCCAAGAGCAGTCTTCTGCCCAGATTCCTTTGCATCAGCAATGGGCTCGCGAAG
GCAAGCGATCCTGTAAGACGAGGCAATGAGCGGGTGCTCAAAGCCAGGCTGGCTGATGCAGAATTCTTTGTCGAAGCCGA
TCGCTCTTTAGCCAGCATCGAGCGGCGCAAAAAGCTAGCGCATGTGACCTTTGCCGAGGGCCTTGGTTCTCTGCTCGATC
GAGTTGAGCGCATGGAATGGCTCACTGATGTGCTTGTTGAGCTGCTGGATCTTTCTGCTGAAATTTCTGTACAAGTGCGT
CGAGCCGTCCATCTCTGTAAACACGATCTGGTTAGTCAGATGGTGGGCGAATTTCCAGAGCTACAGGGCGTGATGGGAGC
GAAATATTTGTTAGCGGAAGGAGAGCCTCGAGAAGTAGCCCTTGCCGTTTTGGAGCATTACCTGCCACGTGGGGCAGGGG
ATGCTTTGCCCGAGTCAGAGTCGGGAGCAGTGCTTGCCCTGGCCGATCGCCTGGAATTGCTGCTCAGCATCTATGCCAAG
GGTGACCGTCCTAGTGGCTCCTCAGACCCCTATGCCTTGCGCAGGGCCGGCAACGGTCTTTTGCAAATCCTTTGGTCGAA
GGGTTGGCGACTGAATCTGCTCGCCTTATTGCAACGAGCGACGAACCATTGGAGCAAGCTCCTACCGCATCTCAATGTCA
CACCATCCGCCTTGGCGGCAGAGCTTGGTGAGTTTCTTCGCCAGCGGATGCTCAGCCTGCTGGAGGAGGCAGGAACCGAT
ATTGATCTGGCCCAAGCGGTTGCTGGCGAAACAGTTTTGATCGATCGTCTTCTTTCTGACCCTGCAGATGCGCGACTGCG
AGTGGATCTGCTCGTGAGGATGCGCCGCACGGGTAAGTTGCTGGCGGTTCAGGCTGTTGTTACCAGAGCAGCACGCTTAG
CAGAGAAAGGGGACCTACCAGCAACCGTACTGAGTGCGGCTGAGGTCGTTGATCCTGGTTTGTTTGAAAAGCCTAGTGAG
TTGGAGATGTTGAAAGTGCTCAATGCTCTTGAACCAATCACCAAGGATTCCTCAAGTGAACGCTACCGCCATTTAGCAGA
TGGCCTGATTGCTGGCTCGGAAGCTCTCGCAGCTTTTTTTGATGGTGATCAGAGTGTGATGGTCATGAGTGAAGATCTCG
CTGTACGCGCAAACCGTCTCAATCTGCTTAGCCTTTTGCGCAACCAAGCTGGTGTGCTGGCCGATTTCAGCCAAATCAGC
GGCTAA

Upstream 100 bases:

>100_bases
TCACGGCGTCAAAATTTTCCTAATACACCGTAGCGAAATGCACACGACCTGTTGAGCTGCTCCCTTCAATACAGTTGGGC
GGTCTTGATTGCTCGCCTCC

Downstream 100 bases:

>100_bases
AAGTGGTTATTTCAGGCTTAGGATTTGTTTGAAACAGCAGGGTCACGTTCTTCTTGTTCTGATGCCAAATTTTGCTTGGA
GGCATCTTTGTTGTTGTCTA

Product: glycyl-tRNA synthetase, beta subunit

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS [H]

Number of amino acids: Translated: 721; Mature: 720

Protein sequence:

>721_residues
MSTLLVEIGTEELPADFARLALPQLEQMVSRDLQGLRLSYGMLQCTSTPRRLVVLVENLASATADLEEVRKGPPAGQAFK
NGVPTQAAAGFAKRCGLKAEDLEIRETPKGPFVFATVLEHGRSAMELLTDQIPEWIGSLQGRRFMRWGEGERRFSRPIRW
LVALLDEQIIPVCLDGSDPEICAANLSRGHRLHDQAVIIPSAESYVQTLAKVGVEVDRQKRGTLIRHAIDTAAAGLEACP
NCPDDLFEELTDLVEAPLLLEGTFAKSYLDLPAEVLSTVMRVHQRYVPLYRLDAVNDPLALDAKSSLLPRFLCISNGLAK
ASDPVRRGNERVLKARLADAEFFVEADRSLASIERRKKLAHVTFAEGLGSLLDRVERMEWLTDVLVELLDLSAEISVQVR
RAVHLCKHDLVSQMVGEFPELQGVMGAKYLLAEGEPREVALAVLEHYLPRGAGDALPESESGAVLALADRLELLLSIYAK
GDRPSGSSDPYALRRAGNGLLQILWSKGWRLNLLALLQRATNHWSKLLPHLNVTPSALAAELGEFLRQRMLSLLEEAGTD
IDLAQAVAGETVLIDRLLSDPADARLRVDLLVRMRRTGKLLAVQAVVTRAARLAEKGDLPATVLSAAEVVDPGLFEKPSE
LEMLKVLNALEPITKDSSSERYRHLADGLIAGSEALAAFFDGDQSVMVMSEDLAVRANRLNLLSLLRNQAGVLADFSQIS
G

Sequences:

>Translated_721_residues
MSTLLVEIGTEELPADFARLALPQLEQMVSRDLQGLRLSYGMLQCTSTPRRLVVLVENLASATADLEEVRKGPPAGQAFK
NGVPTQAAAGFAKRCGLKAEDLEIRETPKGPFVFATVLEHGRSAMELLTDQIPEWIGSLQGRRFMRWGEGERRFSRPIRW
LVALLDEQIIPVCLDGSDPEICAANLSRGHRLHDQAVIIPSAESYVQTLAKVGVEVDRQKRGTLIRHAIDTAAAGLEACP
NCPDDLFEELTDLVEAPLLLEGTFAKSYLDLPAEVLSTVMRVHQRYVPLYRLDAVNDPLALDAKSSLLPRFLCISNGLAK
ASDPVRRGNERVLKARLADAEFFVEADRSLASIERRKKLAHVTFAEGLGSLLDRVERMEWLTDVLVELLDLSAEISVQVR
RAVHLCKHDLVSQMVGEFPELQGVMGAKYLLAEGEPREVALAVLEHYLPRGAGDALPESESGAVLALADRLELLLSIYAK
GDRPSGSSDPYALRRAGNGLLQILWSKGWRLNLLALLQRATNHWSKLLPHLNVTPSALAAELGEFLRQRMLSLLEEAGTD
IDLAQAVAGETVLIDRLLSDPADARLRVDLLVRMRRTGKLLAVQAVVTRAARLAEKGDLPATVLSAAEVVDPGLFEKPSE
LEMLKVLNALEPITKDSSSERYRHLADGLIAGSEALAAFFDGDQSVMVMSEDLAVRANRLNLLSLLRNQAGVLADFSQIS
G
>Mature_720_residues
STLLVEIGTEELPADFARLALPQLEQMVSRDLQGLRLSYGMLQCTSTPRRLVVLVENLASATADLEEVRKGPPAGQAFKN
GVPTQAAAGFAKRCGLKAEDLEIRETPKGPFVFATVLEHGRSAMELLTDQIPEWIGSLQGRRFMRWGEGERRFSRPIRWL
VALLDEQIIPVCLDGSDPEICAANLSRGHRLHDQAVIIPSAESYVQTLAKVGVEVDRQKRGTLIRHAIDTAAAGLEACPN
CPDDLFEELTDLVEAPLLLEGTFAKSYLDLPAEVLSTVMRVHQRYVPLYRLDAVNDPLALDAKSSLLPRFLCISNGLAKA
SDPVRRGNERVLKARLADAEFFVEADRSLASIERRKKLAHVTFAEGLGSLLDRVERMEWLTDVLVELLDLSAEISVQVRR
AVHLCKHDLVSQMVGEFPELQGVMGAKYLLAEGEPREVALAVLEHYLPRGAGDALPESESGAVLALADRLELLLSIYAKG
DRPSGSSDPYALRRAGNGLLQILWSKGWRLNLLALLQRATNHWSKLLPHLNVTPSALAAELGEFLRQRMLSLLEEAGTDI
DLAQAVAGETVLIDRLLSDPADARLRVDLLVRMRRTGKLLAVQAVVTRAARLAEKGDLPATVLSAAEVVDPGLFEKPSEL
EMLKVLNALEPITKDSSSERYRHLADGLIAGSEALAAFFDGDQSVMVMSEDLAVRANRLNLLSLLRNQAGVLADFSQISG

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1789982, Length=723, Percent_Identity=32.7800829875519, Blast_Score=320, Evalue=3e-88,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311 [H]

Pfam domain/function: PF02092 tRNA_synt_2f [H]

EC number: =6.1.1.14 [H]

Molecular weight: Translated: 78999; Mature: 78868

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTLLVEIGTEELPADFARLALPQLEQMVSRDLQGLRLSYGMLQCTSTPRRLVVLVENLA
CCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHH
SATADLEEVRKGPPAGQAFKNGVPTQAAAGFAKRCGLKAEDLEIRETPKGPFVFATVLEH
HHHHHHHHHHCCCCCCHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCEEHHHHHHC
GRSAMELLTDQIPEWIGSLQGRRFMRWGEGERRFSRPIRWLVALLDEQIIPVCLDGSDPE
CHHHHHHHHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCC
ICAANLSRGHRLHDQAVIIPSAESYVQTLAKVGVEVDRQKRGTLIRHAIDTAAAGLEACP
HHHHHHHCCCEECCCEEEECCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCC
NCPDDLFEELTDLVEAPLLLEGTFAKSYLDLPAEVLSTVMRVHQRYVPLYRLDAVNDPLA
CCHHHHHHHHHHHHHCCHHEECHHHHHHHCCCHHHHHHHHHHHHHHCCHHEECCCCCCCE
LDAKSSLLPRFLCISNGLAKASDPVRRGNERVLKARLADAEFFVEADRSLASIERRKKLA
ECCHHHHHHHHHHHHCCCCCCCCHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
HVTFAEGLGSLLDRVERMEWLTDVLVELLDLSAEISVQVRRAVHLCKHDLVSQMVGEFPE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHH
LQGVMGAKYLLAEGEPREVALAVLEHYLPRGAGDALPESESGAVLALADRLELLLSIYAK
HHHHHCCHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHC
GDRPSGSSDPYALRRAGNGLLQILWSKGWRLNLLALLQRATNHWSKLLPHLNVTPSALAA
CCCCCCCCCCHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
ELGEFLRQRMLSLLEEAGTDIDLAQAVAGETVLIDRLLSDPADARLRVDLLVRMRRTGKL
HHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHCCCH
LAVQAVVTRAARLAEKGDLPATVLSAAEVVDPGLFEKPSELEMLKVLNALEPITKDSSSE
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCHH
RYRHLADGLIAGSEALAAFFDGDQSVMVMSEDLAVRANRLNLLSLLRNQAGVLADFSQIS
HHHHHHHHHHCCHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCHHHHHHHHC
G
C
>Mature Secondary Structure 
STLLVEIGTEELPADFARLALPQLEQMVSRDLQGLRLSYGMLQCTSTPRRLVVLVENLA
CEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHH
SATADLEEVRKGPPAGQAFKNGVPTQAAAGFAKRCGLKAEDLEIRETPKGPFVFATVLEH
HHHHHHHHHHCCCCCCHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCEEHHHHHHC
GRSAMELLTDQIPEWIGSLQGRRFMRWGEGERRFSRPIRWLVALLDEQIIPVCLDGSDPE
CHHHHHHHHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCC
ICAANLSRGHRLHDQAVIIPSAESYVQTLAKVGVEVDRQKRGTLIRHAIDTAAAGLEACP
HHHHHHHCCCEECCCEEEECCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCC
NCPDDLFEELTDLVEAPLLLEGTFAKSYLDLPAEVLSTVMRVHQRYVPLYRLDAVNDPLA
CCHHHHHHHHHHHHHCCHHEECHHHHHHHCCCHHHHHHHHHHHHHHCCHHEECCCCCCCE
LDAKSSLLPRFLCISNGLAKASDPVRRGNERVLKARLADAEFFVEADRSLASIERRKKLA
ECCHHHHHHHHHHHHCCCCCCCCHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
HVTFAEGLGSLLDRVERMEWLTDVLVELLDLSAEISVQVRRAVHLCKHDLVSQMVGEFPE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHH
LQGVMGAKYLLAEGEPREVALAVLEHYLPRGAGDALPESESGAVLALADRLELLLSIYAK
HHHHHCCHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHC
GDRPSGSSDPYALRRAGNGLLQILWSKGWRLNLLALLQRATNHWSKLLPHLNVTPSALAA
CCCCCCCCCCHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
ELGEFLRQRMLSLLEEAGTDIDLAQAVAGETVLIDRLLSDPADARLRVDLLVRMRRTGKL
HHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHCCCH
LAVQAVVTRAARLAEKGDLPATVLSAAEVVDPGLFEKPSELEMLKVLNALEPITKDSSSE
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCHH
RYRHLADGLIAGSEALAAFFDGDQSVMVMSEDLAVRANRLNLLSLLRNQAGVLADFSQIS
HHHHHHHHHHCCHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCHHHHHHHHC
G
C

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA