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The map label for this gene is mas1 [H]

Identifier: 33862821

GI number: 33862821

Start: 602635

End: 603345

Strand: Reverse

Name: mas1 [H]

Synonym: PMT0548

Alternate gene names: 33862821

Gene position: 603345-602635 (Counterclockwise)

Preceding gene: 33862822

Following gene: 33862818

Centisome position: 25.03

GC content: 51.76

Gene sequence:

>711_bases
ATGATGCGCACCATCTTGATCAGTGGTGCCAGTCGAGGTATCGGCCGAGCAATTGCCGAACGAATGTTGGCTGATGGCCA
TCGTCTTAGCCTTGGCCTACGGGACCCTAAATGCCTAATTGGCAGCTCACTTGACCCGGCAATCGCTGGTTCTGAACAAG
TGTTCCTACATCCCTATGAAGCTCAGGACGTTAACGCTGCCGAAAGCTGGGTATCGGCAAGCTTGGCTCATTTCAGTGGT
TTCGATAGTTTGATTCACTGTGCAGGCATATTTCATCGCACGCCCATACGGTTTAATGATAACCAGCAGCATGAGATTGA
TCAGCTCTGGCAGGTGAATGTCATGGGCCCATGGATGCTCAGCCGCGCTGCTTGGTCTCAGCTTGAATGTCATGCTGAGG
CGCGCATCATTGTGTTGGTGTCAATGAGTGGCAAGCGCTCCAAGGGCCGCTTGGCTGGCTACACCGTTAGTAAGTTTGCT
CTGATGGGCCTTTGTCAGACCATGCGTAACGAAGGATGGGAGCAAGGCATCCGCGTGACAGCGATTTGTCCAGGTTGGGT
CAATACCGATATGGCTGCCAGGGTTAAGTCTTTGCCCAAAGAGGCGATGACTCAGCCTGATGACATCGCCTCTTTGAGCT
CAAATTTGCTACAGCTACCGAACAGTTGTGTACCGTTTGAACTGAGCATGAACTGCACACATGAGATCTAA

Upstream 100 bases:

>100_bases
GTGGAGCGGGATTTCATCAAAAGGTTTTCAGATTCTGCAGAGTTATGAGAATCACGAGAACTCCCAGCAATAATGTTTTT
GGATTTACGACCATTCAGAA

Downstream 100 bases:

>100_bases
ACCCTTAGTAGAGTTGATGAGACTTTGTTATTGGCAAAGAAATGACGAGCAAAACTTTCCAAACCTCTTAAATAATATGG
ATTCTTTGCGATAAGAATCG

Product: short-chain dehydrogenase/reductase

Products: (3R)-hydroxyacyl-[acyl-carrier-protein]; NADP; NADPH; Proton; beta-ketoacyl-ACP [C]

Alternate protein names: NA

Number of amino acids: Translated: 236; Mature: 236

Protein sequence:

>236_residues
MMRTILISGASRGIGRAIAERMLADGHRLSLGLRDPKCLIGSSLDPAIAGSEQVFLHPYEAQDVNAAESWVSASLAHFSG
FDSLIHCAGIFHRTPIRFNDNQQHEIDQLWQVNVMGPWMLSRAAWSQLECHAEARIIVLVSMSGKRSKGRLAGYTVSKFA
LMGLCQTMRNEGWEQGIRVTAICPGWVNTDMAARVKSLPKEAMTQPDDIASLSSNLLQLPNSCVPFELSMNCTHEI

Sequences:

>Translated_236_residues
MMRTILISGASRGIGRAIAERMLADGHRLSLGLRDPKCLIGSSLDPAIAGSEQVFLHPYEAQDVNAAESWVSASLAHFSG
FDSLIHCAGIFHRTPIRFNDNQQHEIDQLWQVNVMGPWMLSRAAWSQLECHAEARIIVLVSMSGKRSKGRLAGYTVSKFA
LMGLCQTMRNEGWEQGIRVTAICPGWVNTDMAARVKSLPKEAMTQPDDIASLSSNLLQLPNSCVPFELSMNCTHEI
>Mature_236_residues
MMRTILISGASRGIGRAIAERMLADGHRLSLGLRDPKCLIGSSLDPAIAGSEQVFLHPYEAQDVNAAESWVSASLAHFSG
FDSLIHCAGIFHRTPIRFNDNQQHEIDQLWQVNVMGPWMLSRAAWSQLECHAEARIIVLVSMSGKRSKGRLAGYTVSKFA
LMGLCQTMRNEGWEQGIRVTAICPGWVNTDMAARVKSLPKEAMTQPDDIASLSSNLLQLPNSCVPFELSMNCTHEI

Specific function: Reduces deoxy-fructosyl-glutamine to mannopine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI33667109, Length=204, Percent_Identity=29.4117647058824, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI5031737, Length=200, Percent_Identity=29.5, Blast_Score=67, Evalue=1e-11,
Organism=Escherichia coli, GI1787335, Length=187, Percent_Identity=29.4117647058824, Blast_Score=73, Evalue=1e-14,
Organism=Escherichia coli, GI87082100, Length=226, Percent_Identity=26.1061946902655, Blast_Score=68, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI71994604, Length=192, Percent_Identity=29.1666666666667, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI71994600, Length=193, Percent_Identity=26.9430051813472, Blast_Score=68, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6322861, Length=219, Percent_Identity=26.027397260274, Blast_Score=62, Evalue=6e-11,
Organism=Drosophila melanogaster, GI21358495, Length=198, Percent_Identity=25.2525252525253, Blast_Score=68, Evalue=6e-12,
Organism=Drosophila melanogaster, GI28571387, Length=234, Percent_Identity=28.6324786324786, Blast_Score=67, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR013078
- InterPro:   IPR015845
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short; PF00300 PGAM [H]

EC number: 1.1.1.100 [C]

Molecular weight: Translated: 25993; Mature: 25993

Theoretical pI: Translated: 7.19; Mature: 7.19

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
7.6 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
7.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMRTILISGASRGIGRAIAERMLADGHRLSLGLRDPKCLIGSSLDPAIAGSEQVFLHPYE
CCEEEEEECCCCCHHHHHHHHHHCCCCEEEECCCCCHHHHCCCCCCCCCCCCEEEEECCC
AQDVNAAESWVSASLAHFSGFDSLIHCAGIFHRTPIRFNDNQQHEIDQLWQVNVMGPWML
CCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHEEEECCCHHHH
SRAAWSQLECHAEARIIVLVSMSGKRSKGRLAGYTVSKFALMGLCQTMRNEGWEQGIRVT
HHHHHHHHHHCCCEEEEEEEECCCCCCCCCEECHHHHHHHHHHHHHHHHHCCHHCCCEEE
AICPGWVNTDMAARVKSLPKEAMTQPDDIASLSSNLLQLPNSCVPFELSMNCTHEI
EEECCCCCCHHHHHHHHCCHHHCCCCHHHHHHHHHHHHCCCCCCCEEEECCCCCCC
>Mature Secondary Structure
MMRTILISGASRGIGRAIAERMLADGHRLSLGLRDPKCLIGSSLDPAIAGSEQVFLHPYE
CCEEEEEECCCCCHHHHHHHHHHCCCCEEEECCCCCHHHHCCCCCCCCCCCCEEEEECCC
AQDVNAAESWVSASLAHFSGFDSLIHCAGIFHRTPIRFNDNQQHEIDQLWQVNVMGPWML
CCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHEEEECCCHHHH
SRAAWSQLECHAEARIIVLVSMSGKRSKGRLAGYTVSKFALMGLCQTMRNEGWEQGIRVT
HHHHHHHHHHCCCEEEEEEEECCCCCCCCCEECHHHHHHHHHHHHHHHHHCCHHCCCEEE
AICPGWVNTDMAARVKSLPKEAMTQPDDIASLSSNLLQLPNSCVPFELSMNCTHEI
EEECCCCCCHHHHHHHHCCHHHCCCCHHHHHHHHHHHHCCCCCCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Oxoacyl-[acyl-carrier-protein]; NADPH; D-3-hydroxy-acyl-ACP; NADP [C]

Specific reaction: Oxoacyl-[acyl-carrier-protein] + NADPH = (3R)-hydroxyacyl-[acyl-carrier-protein] + NADP+ D-3-hydroxy-acyl-ACP + NADP = NADPH + Proton + beta-ketoacyl-ACP [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1909028 [H]