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The map label for this gene is gadA [H]

Identifier: 33862747

GI number: 33862747

Start: 526282

End: 527721

Strand: Reverse

Name: gadA [H]

Synonym: PMT0474

Alternate gene names: 33862747

Gene position: 527721-526282 (Counterclockwise)

Preceding gene: 33862753

Following gene: 33862746

Centisome position: 21.89

GC content: 52.43

Gene sequence:

>1440_bases
ATGGCCCTGCATCGTTCCAATCCCCGCTCCAACGATCTGGAAGAACATGCCATTGGGGAAACCGTTGATGCATTGGTCTC
ACCATTACCACGCACGACTTTCCCGCAAGAGTACCGTCCTGCATTAACAACATTTAATTTGCTCAAGGAGGAATTATTGC
TTGACGGCAACAGCAAACAAAATCTGGCCACCTTCTGTCAGACCTATGAAAGCCAACAGGTGGCTGAGCTAATGGCCCTC
GCGGTTGACAAGAACCTGATCGACAAGGACGAATATCCCCAAACCGCAGAGATTGAACGCCGTTGTGTTTCGATGATGGC
TGATTTATGGCATGCCCCCGGTGAAGCTGTGGGCTGCTCCACCATTGGTAGCAGTGAGGCCGCCATGCTGGGTGGCTTGG
CTGCGAAATGGCGTTGGCGTGAACATCGCAAAGCGGCAGGTCAGTCGATCGATCGCCCCAACATGGTTTGCGGCAGTGTA
CAAATCTGCTGGACGAAATTTGCCCGCTACTGGGACGTTGAGTTGCGGGAGGTGGAGATGACCTCCGGCGAGCTCTGCAT
GTCTCCAGAACGAATGCTGGAGCAAGTGGATGAGAACACCATCGTTGTGGTACCTACCCTGGGCGTCACTTATCATGGCC
TGTATGAAGACGTACAAGCCGTCAGTCAGGCCCTCGATGAGCTGCAACAGCGAAAAGGACTCGACATTCCTATCCATGTG
GATGCAGCCAGCGGTGGCTTTCTTGCTCCTTTCTGTGCCCCTGATCTAGCTCCCTGGGATTTCCGCTTGCCGCGCGTGAA
GTCAATCAACTCCTCTGGCCATAAGTTCGGCTTAGCTCCTCTGGGTGTGGGTTGGGTTCTTTGGCGTGAGTCCCGTGATC
TACCGGAAGGATTGGTTTTCCATGTGAGTTACCTAGGAGGCGATATGCCAACGTTTCAGATCAACTTCTCGCGGCCAGCG
GGGCAGGTGATCTCCCAGTACTACGACTTTGTCCGCCTAGGTCGCGATGGTTATCAAGCCATCCATGGAGCGAGCTATGC
CAATGCTCAATATGTTGCTCAAGAACTCAAAAAGCTAGGCCCATTTGAGTTGATAAACGATGGCAACCCAGCAGGTGGCA
TCCCCACGGTGGTATGGACATTGCGAGCCGACCAAGAGCTTGGTTTTAACCTTTACGACCTCTCTGATCGACTGAGATTA
CGTGGTTGGCAAGTGCCAGCTTATCCATTCACAGGTGAGCTTGCTCATCAAGCCTTTCAGCGGATCTTGGTAAAACGCGA
TTTCTCACGTGAAATGGCTGATCTCCTACTTACCGACATCCGCAACGCCATAACTCATTTCGAGAGTCATCCTGTCAAGA
TCAGTCTTAACGCTAATGAAGCAGCGTCTACTAATCATCTCGGCCGTTCAATGGTTGAATGCCGTGATGCGCATGGATGA

Upstream 100 bases:

>100_bases
CATCAACCGACTCAGTTGTTTGTTATTGCACCTCCCATCTTGCAAGGCTTCCTTGCTCAAACAACGTCGTTAAGGTCATA
CATAGAAACAAGAAAAAAGC

Downstream 100 bases:

>100_bases
TGCCCCACTATTGGGGGCTTTGAATTTAAAGCGGGAGCATCCTCCAAACCTTCAGTCGCATTATTCAAGTCCATGGGGCA
CGACAGCTTGGCGGGAAGAT

Product: glutamate decarboxylase

Products: NA

Alternate protein names: GAD-alpha [H]

Number of amino acids: Translated: 479; Mature: 478

Protein sequence:

>479_residues
MALHRSNPRSNDLEEHAIGETVDALVSPLPRTTFPQEYRPALTTFNLLKEELLLDGNSKQNLATFCQTYESQQVAELMAL
AVDKNLIDKDEYPQTAEIERRCVSMMADLWHAPGEAVGCSTIGSSEAAMLGGLAAKWRWREHRKAAGQSIDRPNMVCGSV
QICWTKFARYWDVELREVEMTSGELCMSPERMLEQVDENTIVVVPTLGVTYHGLYEDVQAVSQALDELQQRKGLDIPIHV
DAASGGFLAPFCAPDLAPWDFRLPRVKSINSSGHKFGLAPLGVGWVLWRESRDLPEGLVFHVSYLGGDMPTFQINFSRPA
GQVISQYYDFVRLGRDGYQAIHGASYANAQYVAQELKKLGPFELINDGNPAGGIPTVVWTLRADQELGFNLYDLSDRLRL
RGWQVPAYPFTGELAHQAFQRILVKRDFSREMADLLLTDIRNAITHFESHPVKISLNANEAASTNHLGRSMVECRDAHG

Sequences:

>Translated_479_residues
MALHRSNPRSNDLEEHAIGETVDALVSPLPRTTFPQEYRPALTTFNLLKEELLLDGNSKQNLATFCQTYESQQVAELMAL
AVDKNLIDKDEYPQTAEIERRCVSMMADLWHAPGEAVGCSTIGSSEAAMLGGLAAKWRWREHRKAAGQSIDRPNMVCGSV
QICWTKFARYWDVELREVEMTSGELCMSPERMLEQVDENTIVVVPTLGVTYHGLYEDVQAVSQALDELQQRKGLDIPIHV
DAASGGFLAPFCAPDLAPWDFRLPRVKSINSSGHKFGLAPLGVGWVLWRESRDLPEGLVFHVSYLGGDMPTFQINFSRPA
GQVISQYYDFVRLGRDGYQAIHGASYANAQYVAQELKKLGPFELINDGNPAGGIPTVVWTLRADQELGFNLYDLSDRLRL
RGWQVPAYPFTGELAHQAFQRILVKRDFSREMADLLLTDIRNAITHFESHPVKISLNANEAASTNHLGRSMVECRDAHG
>Mature_478_residues
ALHRSNPRSNDLEEHAIGETVDALVSPLPRTTFPQEYRPALTTFNLLKEELLLDGNSKQNLATFCQTYESQQVAELMALA
VDKNLIDKDEYPQTAEIERRCVSMMADLWHAPGEAVGCSTIGSSEAAMLGGLAAKWRWREHRKAAGQSIDRPNMVCGSVQ
ICWTKFARYWDVELREVEMTSGELCMSPERMLEQVDENTIVVVPTLGVTYHGLYEDVQAVSQALDELQQRKGLDIPIHVD
AASGGFLAPFCAPDLAPWDFRLPRVKSINSSGHKFGLAPLGVGWVLWRESRDLPEGLVFHVSYLGGDMPTFQINFSRPAG
QVISQYYDFVRLGRDGYQAIHGASYANAQYVAQELKKLGPFELINDGNPAGGIPTVVWTLRADQELGFNLYDLSDRLRLR
GWQVPAYPFTGELAHQAFQRILVKRDFSREMADLLLTDIRNAITHFESHPVKISLNANEAASTNHLGRSMVECRDAHG

Specific function: Converts glutamate to gamma-aminobutyrate (GABA), consuming one intracellular proton in the reaction. The gad system helps to maintain a near-neutral intracellular pH when cells are exposed to extremely acidic conditions. The ability to survive transit th

COG id: COG0076

COG function: function code E; Glutamate decarboxylase and related PLP-dependent proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the group II decarboxylase family [H]

Homologues:

Organism=Homo sapiens, GI31982936, Length=473, Percent_Identity=24.1014799154334, Blast_Score=101, Evalue=2e-21,
Organism=Escherichia coli, GI1787769, Length=413, Percent_Identity=59.5641646489104, Blast_Score=527, Evalue=1e-151,
Organism=Escherichia coli, GI1789934, Length=413, Percent_Identity=59.5641646489104, Blast_Score=526, Evalue=1e-151,
Organism=Caenorhabditis elegans, GI17557272, Length=372, Percent_Identity=25.5376344086022, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17543922, Length=326, Percent_Identity=25.4601226993865, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI25148342, Length=416, Percent_Identity=24.2788461538462, Blast_Score=93, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6323905, Length=465, Percent_Identity=38.0645161290323, Blast_Score=296, Evalue=3e-81,
Organism=Saccharomyces cerevisiae, GI6320500, Length=377, Percent_Identity=25.9946949602122, Blast_Score=114, Evalue=4e-26,
Organism=Drosophila melanogaster, GI21355963, Length=384, Percent_Identity=23.9583333333333, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI24654344, Length=384, Percent_Identity=23.9583333333333, Blast_Score=112, Evalue=5e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010107
- InterPro:   IPR002129
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR021115 [H]

Pfam domain/function: PF00282 Pyridoxal_deC [H]

EC number: =4.1.1.15 [H]

Molecular weight: Translated: 53548; Mature: 53417

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALHRSNPRSNDLEEHAIGETVDALVSPLPRTTFPQEYRPALTTFNLLKEELLLDGNSKQ
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCH
NLATFCQTYESQQVAELMALAVDKNLIDKDEYPQTAEIERRCVSMMADLWHAPGEAVGCS
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCC
TIGSSEAAMLGGLAAKWRWREHRKAAGQSIDRPNMVCGSVQICWTKFARYWDVELREVEM
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECHHHHHHHHHHHHHCCEEEEEEC
TSGELCMSPERMLEQVDENTIVVVPTLGVTYHGLYEDVQAVSQALDELQQRKGLDIPIHV
CCCCCCCCHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
DAASGGFLAPFCAPDLAPWDFRLPRVKSINSSGHKFGLAPLGVGWVLWRESRDLPEGLVF
ECCCCCEECHHCCCCCCCCCCCCCCHHCCCCCCCEECCCCCCCCEEEEECCCCCCCHHEE
HVSYLGGDMPTFQINFSRPAGQVISQYYDFVRLGRDGYQAIHGASYANAQYVAQELKKLG
EEHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHHHCC
PFELINDGNPAGGIPTVVWTLRADQELGFNLYDLSDRLRLRGWQVPAYPFTGELAHQAFQ
CEEEECCCCCCCCCCEEEEEEECCHHHCCCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHH
RILVKRDFSREMADLLLTDIRNAITHFESHPVKISLNANEAASTNHLGRSMVECRDAHG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
ALHRSNPRSNDLEEHAIGETVDALVSPLPRTTFPQEYRPALTTFNLLKEELLLDGNSKQ
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCH
NLATFCQTYESQQVAELMALAVDKNLIDKDEYPQTAEIERRCVSMMADLWHAPGEAVGCS
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCC
TIGSSEAAMLGGLAAKWRWREHRKAAGQSIDRPNMVCGSVQICWTKFARYWDVELREVEM
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECHHHHHHHHHHHHHCCEEEEEEC
TSGELCMSPERMLEQVDENTIVVVPTLGVTYHGLYEDVQAVSQALDELQQRKGLDIPIHV
CCCCCCCCHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
DAASGGFLAPFCAPDLAPWDFRLPRVKSINSSGHKFGLAPLGVGWVLWRESRDLPEGLVF
ECCCCCEECHHCCCCCCCCCCCCCCHHCCCCCCCEECCCCCCCCEEEEECCCCCCCHHEE
HVSYLGGDMPTFQINFSRPAGQVISQYYDFVRLGRDGYQAIHGASYANAQYVAQELKKLG
EEHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHHHCC
PFELINDGNPAGGIPTVVWTLRADQELGFNLYDLSDRLRLRGWQVPAYPFTGELAHQAFQ
CEEEECCCCCCCCCCEEEEEEECCHHHCCCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHH
RILVKRDFSREMADLLLTDIRNAITHFESHPVKISLNANEAASTNHLGRSMVECRDAHG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]