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The map label for this gene is murI [H]

Identifier: 33862666

GI number: 33862666

Start: 441562

End: 442368

Strand: Reverse

Name: murI [H]

Synonym: PMT0393

Alternate gene names: 33862666

Gene position: 442368-441562 (Counterclockwise)

Preceding gene: 33862667

Following gene: 33862665

Centisome position: 18.35

GC content: 55.64

Gene sequence:

>807_bases
TTGAAGCCTCGCTTGGGATTATTTGATAGCGGCGTGGGTGGGTTGACTGTGCTCAGGCGAGTGCTGGAGCGCCATGGGCA
GGTGCCTTGCCTCTATCTCGGTGATACCGCCCGAGTCCCTTACGGAAACCGCATGCCGCATGAAATCCGAGTGATCGCCA
AGGAGGTTGTCCAGTGGTTACGAGACCAGCAAGTGACTGCGGTTGTGATGGCTTGCAACACCACGAATGCGTTGGCTTCT
GATGTGGCTGAAGCGGTTGCGGGAAGGATCCCTGTCGTGAGGTTGATTGAGGCAGCAGCCAACATGCTTTCGGAAGAGCG
TGTGGGAGTACTGGCCACTCCTGCTGCTGCTGCATCAGGCGCATATGGCAAACAAATTGAGATCTCTCGACCAGGCACGA
TGGTTATTGAGCAGGGATGTCCGGCCTTTGTGCCATTGATTGAGACTGGTCAACTCAGCAGCGAGGAGCTTTATCAGGCG
GCGCGTGAGTATCTGAATCCACTCTTGGCTGCACAGGTGGAAGCAGTGGTTCTCGGATGCACTCACTATCCTCTTCTTGA
ACCGATCTTACGTCAGATTTTGCCGCAAGATGTGCGCTTGATTGATCCTGCTATCGGTGTGGCCCGCGAGCTGGATGTTC
TGTTGGGTAGCCCAACGATCCCATTAGGAACATCACTGTCGCTGACCTCTACACGGTTCTGCGTGACTGCCAATCCCGAG
GGCTTTGCCACGCGTGCTACGCCATGGTTGGGCGAGCGTCCTCAAGTTGAGTTGGTTTCACTGCAATGTCCTGCCTGCGC
CTCGTAA

Upstream 100 bases:

>100_bases
CAGGACGGCTAGATGCTCCTCGGCTGGCTTCAGCAGCCCATCGCCGCAAGTTGGCTCTGGCTATTGCTACTGGCATCCTC
AACTATTTGCAGGGGGTCCG

Downstream 100 bases:

>100_bases
GATCACTCCACCGAGGGAATCCATGGCCACCGTTACTGAGCTGCTGCAGCCGGTCGAGCTGGATCTTGAGACCCTGCTCA
CTGATCTGCGCAGCTTGATT

Product: aspartate and glutamate racemase:glutamate racemase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MKPRLGLFDSGVGGLTVLRRVLERHGQVPCLYLGDTARVPYGNRMPHEIRVIAKEVVQWLRDQQVTAVVMACNTTNALAS
DVAEAVAGRIPVVRLIEAAANMLSEERVGVLATPAAAASGAYGKQIEISRPGTMVIEQGCPAFVPLIETGQLSSEELYQA
AREYLNPLLAAQVEAVVLGCTHYPLLEPILRQILPQDVRLIDPAIGVARELDVLLGSPTIPLGTSLSLTSTRFCVTANPE
GFATRATPWLGERPQVELVSLQCPACAS

Sequences:

>Translated_268_residues
MKPRLGLFDSGVGGLTVLRRVLERHGQVPCLYLGDTARVPYGNRMPHEIRVIAKEVVQWLRDQQVTAVVMACNTTNALAS
DVAEAVAGRIPVVRLIEAAANMLSEERVGVLATPAAAASGAYGKQIEISRPGTMVIEQGCPAFVPLIETGQLSSEELYQA
AREYLNPLLAAQVEAVVLGCTHYPLLEPILRQILPQDVRLIDPAIGVARELDVLLGSPTIPLGTSLSLTSTRFCVTANPE
GFATRATPWLGERPQVELVSLQCPACAS
>Mature_268_residues
MKPRLGLFDSGVGGLTVLRRVLERHGQVPCLYLGDTARVPYGNRMPHEIRVIAKEVVQWLRDQQVTAVVMACNTTNALAS
DVAEAVAGRIPVVRLIEAAANMLSEERVGVLATPAAAASGAYGKQIEISRPGTMVIEQGCPAFVPLIETGQLSSEELYQA
AREYLNPLLAAQVEAVVLGCTHYPLLEPILRQILPQDVRLIDPAIGVARELDVLLGSPTIPLGTSLSLTSTRFCVTANPE
GFATRATPWLGERPQVELVSLQCPACAS

Specific function: Provides the (R)-glutamate required for cell wall biosynthesis [H]

COG id: COG0796

COG function: function code M; Glutamate racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aspartate/glutamate racemases family [H]

Homologues:

Organism=Escherichia coli, GI87082355, Length=225, Percent_Identity=31.5555555555556, Blast_Score=64, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015942
- InterPro:   IPR001920
- InterPro:   IPR018187
- InterPro:   IPR004391 [H]

Pfam domain/function: PF01177 Asp_Glu_race [H]

EC number: =5.1.1.3 [H]

Molecular weight: Translated: 28727; Mature: 28727

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS00924 ASP_GLU_RACEMASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPRLGLFDSGVGGLTVLRRVLERHGQVPCLYLGDTARVPYGNRMPHEIRVIAKEVVQWL
CCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHH
RDQQVTAVVMACNTTNALASDVAEAVAGRIPVVRLIEAAANMLSEERVGVLATPAAAASG
HCCCEEEEEEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCEEECCCHHCCC
AYGKQIEISRPGTMVIEQGCPAFVPLIETGQLSSEELYQAAREYLNPLLAAQVEAVVLGC
CCCCEEEECCCCCEEEECCCCCEECEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHEECC
THYPLLEPILRQILPQDVRLIDPAIGVARELDVLLGSPTIPLGTSLSLTSTRFCVTANPE
CCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCEEEEEEECCC
GFATRATPWLGERPQVELVSLQCPACAS
CCEECCCCCCCCCCCEEEEEECCCCCCC
>Mature Secondary Structure
MKPRLGLFDSGVGGLTVLRRVLERHGQVPCLYLGDTARVPYGNRMPHEIRVIAKEVVQWL
CCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHH
RDQQVTAVVMACNTTNALASDVAEAVAGRIPVVRLIEAAANMLSEERVGVLATPAAAASG
HCCCEEEEEEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCEEECCCHHCCC
AYGKQIEISRPGTMVIEQGCPAFVPLIETGQLSSEELYQAAREYLNPLLAAQVEAVVLGC
CCCCEEEECCCCCEEEECCCCCEECEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHEECC
THYPLLEPILRQILPQDVRLIDPAIGVARELDVLLGSPTIPLGTSLSLTSTRFCVTANPE
CCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCEEEEEEECCC
GFATRATPWLGERPQVELVSLQCPACAS
CCEECCCCCCCCCCCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA