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The map label for this gene is trmD [H]

Identifier: 33862629

GI number: 33862629

Start: 405664

End: 406896

Strand: Direct

Name: trmD [H]

Synonym: PMT0356

Alternate gene names: 33862629

Gene position: 405664-406896 (Clockwise)

Preceding gene: 33862628

Following gene: 33862630

Centisome position: 16.83

GC content: 54.83

Gene sequence:

>1233_bases
ATGGCTCCTTTTCGCCTCGATGTAGTGACTTTGGTGCCACAGGCATTTACGCCTCTTGGTGAACTCGGGGTAATTGGTCG
TGCTTTTGCCTCTGGCATCGCTGTCTTGCACACCCATAATCCTCGCGATTACACCACGGATCGCTACCACAAGGTTGATG
ATCAGCCCTATGGCGGTGGTGCTGGCATGGTGCTTAAGCCAGAACCAGTCTTTGCTGCTGTTGAGGCGATTCCTGTTCAG
GCGCGACGGCGGGTGCTGCTTCTGACTCCTCAGGGAAAACCTCTATGCCAGAAGGATTTGCATCGTTGGGTAGAGGACCA
CGATCAGCTTGTGCTGATCTGCGGTCATTACGAAGGTTTTGATGAGCGGATTAGAGGTTTGGCAGATGAGGAGGTGTCTG
TAGGGGATTTTGTGCTCACCGGTGGTGAGCTGCCAGCCATGACGGTGATCAATGGGGTGGTGAGGTTGTTACCGGGAACT
GTTGGAACGGCAGAGTCGTTGCAGGATGAGAGTCATAGCGATGTTCTCCTCGAGCATCCTCACTACACAAGGCCAGCGAA
TTTTCGCGGCATGGTTGTTCCAGATGTGTTGCGCAGTGGTGATCATGGTGCGATTGCTCTGTGGCGACAGCAACAGCGGC
AGCTGCGTACGCAGATGCGTCGTCCGGATCTCTATGTTCGCTGGTCTGATCAGCAACAATCGTCAACACTGACAATGGAG
TCCCATGGTTCTACCTCCATGCAGTTTCGTATCGGCAACGGGTATGACATTCATCGCTTAGTGCCCGGCCGCCCCTTGAT
TCTTGGTGGCGTGACACTTGATCATCCCGATGGGTTGGGGCTCGATGGTCACAGCGATGCTGATGTTCTTGTTCATGCGC
TGATGGATGCCTTGCTTGGGGCTCTTGCTTTAGGCGATATCGGTAAATATTTTCCGCCGGATGATCCCCGCTGGAAAGGG
GCCGATAGCTTGATGCTGCTTGAGCAGGTTGTGGCTCTGGTACGAGATCGCGGCTGGCAGATTTTGAATGTGGATGCTGT
GGTGGTGGCTGAGCGTCCCAAGCTCAAGCCTCACATCAATGCGATGCGCAGCAACCTGGCACAACGGTTGGGGGTGGAAT
TGGATGCCGTGGGTGTGAAGGCCACCACCAATGAAGGGCTTGGACCGGAAGGTCGAGAGGAGGGGATGAGTAGTCAGGCA
GTCGCACTGTTGCAGCAGATAGGCCTGACATGA

Upstream 100 bases:

>100_bases
GGCTGAAGTTATTTCTTCAGGGATGCTCTGATCAGCGGATGAGCTTGCACAGCGTCTGTACGCAGATCCTTTGGTTCTCC
CATTTAGCGGCCTGAAGGTG

Downstream 100 bases:

>100_bases
ATTGCAGAAGCTTGTCTAGGTTGATTTCCTTTGTTCTTAGCTTTCTAGTGATGTTGTCCGGTCATCCTGATGTCGCGATT
GCCGCTCGTCATGATCCTGA

Product: bifuntional enzyme: tRNA methyltransferase; 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase [H]

Number of amino acids: Translated: 410; Mature: 409

Protein sequence:

>410_residues
MAPFRLDVVTLVPQAFTPLGELGVIGRAFASGIAVLHTHNPRDYTTDRYHKVDDQPYGGGAGMVLKPEPVFAAVEAIPVQ
ARRRVLLLTPQGKPLCQKDLHRWVEDHDQLVLICGHYEGFDERIRGLADEEVSVGDFVLTGGELPAMTVINGVVRLLPGT
VGTAESLQDESHSDVLLEHPHYTRPANFRGMVVPDVLRSGDHGAIALWRQQQRQLRTQMRRPDLYVRWSDQQQSSTLTME
SHGSTSMQFRIGNGYDIHRLVPGRPLILGGVTLDHPDGLGLDGHSDADVLVHALMDALLGALALGDIGKYFPPDDPRWKG
ADSLMLLEQVVALVRDRGWQILNVDAVVVAERPKLKPHINAMRSNLAQRLGVELDAVGVKATTNEGLGPEGREEGMSSQA
VALLQQIGLT

Sequences:

>Translated_410_residues
MAPFRLDVVTLVPQAFTPLGELGVIGRAFASGIAVLHTHNPRDYTTDRYHKVDDQPYGGGAGMVLKPEPVFAAVEAIPVQ
ARRRVLLLTPQGKPLCQKDLHRWVEDHDQLVLICGHYEGFDERIRGLADEEVSVGDFVLTGGELPAMTVINGVVRLLPGT
VGTAESLQDESHSDVLLEHPHYTRPANFRGMVVPDVLRSGDHGAIALWRQQQRQLRTQMRRPDLYVRWSDQQQSSTLTME
SHGSTSMQFRIGNGYDIHRLVPGRPLILGGVTLDHPDGLGLDGHSDADVLVHALMDALLGALALGDIGKYFPPDDPRWKG
ADSLMLLEQVVALVRDRGWQILNVDAVVVAERPKLKPHINAMRSNLAQRLGVELDAVGVKATTNEGLGPEGREEGMSSQA
VALLQQIGLT
>Mature_409_residues
APFRLDVVTLVPQAFTPLGELGVIGRAFASGIAVLHTHNPRDYTTDRYHKVDDQPYGGGAGMVLKPEPVFAAVEAIPVQA
RRRVLLLTPQGKPLCQKDLHRWVEDHDQLVLICGHYEGFDERIRGLADEEVSVGDFVLTGGELPAMTVINGVVRLLPGTV
GTAESLQDESHSDVLLEHPHYTRPANFRGMVVPDVLRSGDHGAIALWRQQQRQLRTQMRRPDLYVRWSDQQQSSTLTMES
HGSTSMQFRIGNGYDIHRLVPGRPLILGGVTLDHPDGLGLDGHSDADVLVHALMDALLGALALGDIGKYFPPDDPRWKGA
DSLMLLEQVVALVRDRGWQILNVDAVVVAERPKLKPHINAMRSNLAQRLGVELDAVGVKATTNEGLGPEGREEGMSSQAV
ALLQQIGLT

Specific function: Specifically methylates guanosine-37 in various tRNAs [H]

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family [H]

Homologues:

Organism=Escherichia coli, GI1788959, Length=246, Percent_Identity=39.8373983739837, Blast_Score=184, Evalue=8e-48,
Organism=Escherichia coli, GI1789103, Length=156, Percent_Identity=51.2820512820513, Blast_Score=137, Evalue=2e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148 [H]

Pfam domain/function: PF01746 tRNA_m1G_MT [H]

EC number: =2.1.1.31 [H]

Molecular weight: Translated: 44894; Mature: 44763

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPFRLDVVTLVPQAFTPLGELGVIGRAFASGIAVLHTHNPRDYTTDRYHKVDDQPYGGG
CCCCEEHHHHHCHHHHCCCHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCC
AGMVLKPEPVFAAVEAIPVQARRRVLLLTPQGKPLCQKDLHRWVEDHDQLVLICGHYEGF
CCEEECCCCHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCEEEEEECCCCH
DERIRGLADEEVSVGDFVLTGGELPAMTVINGVVRLLPGTVGTAESLQDESHSDVLLEHP
HHHHCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCHHHHCCCCCCCEEEECC
HYTRPANFRGMVVPDVLRSGDHGAIALWRQQQRQLRTQMRRPDLYVRWSDQQQSSTLTME
CCCCCCCCCCEECHHHHCCCCCCEEHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEE
SHGSTSMQFRIGNGYDIHRLVPGRPLILGGVTLDHPDGLGLDGHSDADVLVHALMDALLG
CCCCCEEEEEECCCCEEEEECCCCCEEEECEEEECCCCCCCCCCCCHHHHHHHHHHHHHH
ALALGDIGKYFPPDDPRWKGADSLMLLEQVVALVRDRGWQILNVDAVVVAERPKLKPHIN
HHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEEEEECCCCCCHHHH
AMRSNLAQRLGVELDAVGVKATTNEGLGPEGREEGMSSQAVALLQQIGLT
HHHHHHHHHHCCEEEEEEEEECCCCCCCCCCHHHCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
APFRLDVVTLVPQAFTPLGELGVIGRAFASGIAVLHTHNPRDYTTDRYHKVDDQPYGGG
CCCEEHHHHHCHHHHCCCHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCC
AGMVLKPEPVFAAVEAIPVQARRRVLLLTPQGKPLCQKDLHRWVEDHDQLVLICGHYEGF
CCEEECCCCHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCEEEEEECCCCH
DERIRGLADEEVSVGDFVLTGGELPAMTVINGVVRLLPGTVGTAESLQDESHSDVLLEHP
HHHHCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCHHHHCCCCCCCEEEECC
HYTRPANFRGMVVPDVLRSGDHGAIALWRQQQRQLRTQMRRPDLYVRWSDQQQSSTLTME
CCCCCCCCCCEECHHHHCCCCCCEEHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEE
SHGSTSMQFRIGNGYDIHRLVPGRPLILGGVTLDHPDGLGLDGHSDADVLVHALMDALLG
CCCCCEEEEEECCCCEEEEECCCCCEEEECEEEECCCCCCCCCCCCHHHHHHHHHHHHHH
ALALGDIGKYFPPDDPRWKGADSLMLLEQVVALVRDRGWQILNVDAVVVAERPKLKPHIN
HHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEEEEECCCCCCHHHH
AMRSNLAQRLGVELDAVGVKATTNEGLGPEGREEGMSSQAVALLQQIGLT
HHHHHHHHHHCCEEEEEEEEECCCCCCCCCCHHHCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA