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The map label for this gene is nadB [H]

Identifier: 33862450

GI number: 33862450

Start: 200241

End: 201911

Strand: Reverse

Name: nadB [H]

Synonym: PMT0177

Alternate gene names: 33862450

Gene position: 201911-200241 (Counterclockwise)

Preceding gene: 33862451

Following gene: 33862448

Centisome position: 8.38

GC content: 56.97

Gene sequence:

>1671_bases
ATGAGTATCTCTAACCCATCCACGCCCATTCCTAACGGTCCCTGGGATGTCGTGGTGATCGGCGCAGGGGCAGCAGGGCT
GATGACCTGCCTTGAGCTGCCTGCTCAGCTGAAGGTGCTTTTGCTGAACCGCAACACCAGCCGCCACTCCTCCAGCCGCT
GGGCTCAAGGTGGCATCGCCGCAGTAACAAGACCTAAAGACAGCGCCGAGAGCCATGCAGAAGACACTCTCAAAGCAGGC
GCAGGACTCTGTGATGCTGATGCTGTCAGACAACTTGTAGAAGAAGCTCCCCACTGCGTTCAACGTCTCCAGAAGCTTGG
GATGGCCTTTGACCGCGAAGGTGATGCGCTAGCCACCACCTTGGAGGCAGCCCATAGCCATCGAAGAGTGCTGCATGTGC
AGGACCAAACTGGCCGCGCCTTGGTTGAAGCACTGCAAGAGCAAGTGGAACGGCGCCCCAACCTCTTGCACCGGCGAGGG
GTACGCGCCACCCAGCTTTGGATTGAGCACAATCGCTGCTACGGGGTACAAGTGCTCGACGGAACGCTGCTCCATTGGCT
TTCGGCCAGAGCGGTAGTCCTGGCAACTGGTGGCGGTGGTCATTTGTTCGCAAACACCACCAATCCGGCTCAGGCCTGCG
GAGAAGGCTTAGCCCTTGCCTGGCAAGCAGGCGCCACGATCGAGGATCTTGAATTCGTGCAGTTTCATCCCACAGCTCTG
AAACTGGAAGGAGCTCCATGCTTTCTGATTTCAGAAGCAGTCAGAGGCGAAGGTGCCGTACTGGTTGATGGCCAAGGGCA
AAGTCCTGTAAAGGAATTGAGCGCTGGTGATCTTGCTCCCCGCGACCAAGTAAGCCGGTCCCTCATCCAAACCATGCATC
ACCAAGGGGTCCAGCATCTTGGCCTAGATCTCACACCCATCCCTTCAGGGCAGGCCGAGGTCCGTTTCCCAACCATCCTG
GAACGCTGTCGGGAATTAGGTTTAGATCCACTCAACCAACCGATCCCGGTAGCCCCAGCAGCTCACTACTGGATGGGAGG
AATCGCCACCAACCTTCAAGCCGCAACCAATCTTCCAGGGCTCTATGCGATTGGGGAAGTGGCCTGCACCGGAGTGCATG
GAGCCAACCGACTCGCTGGCAACTCGCTGATGGAATGCATGGTATTTGCCAGACAAATGGCCACAATCGACCTGGGCTCT
TTGCCTCGTCTGGGTGTTCATCAAAGAGATTCTTCCCCCGAGCCTTGCTGCCAGCAACTCCATGGCACTGCAACTGCCGA
GTCTCTAACTCGCGCCATCGCAGATCTGCGAGAACTTTGCTGGCGAACGGCCGGTGTAGATCGCTCTGCCAAGGGAATGA
AACAAAGCCTCGCGAGCATCCGTAAGGATCTACAGCACATTGGTGAACAGCCTTTGCTCAAGCTGGTAAGCCATCAGCCT
CAAGACATTTGCCATCACCTCGATGACACAGCCAGAAGGGACCTCAACTTGCTTCTGGACCTCAACCATCGCCAGTTGAC
CAGCTCCTTAATGCTGGAAGCCTGTTTGTTCCGCCGCGAAAGTCGCGGCGGACATTTCAGAACTGATGCCCCTGCAGAAA
TGCCTCAATGGCAACGCCACTCACGGCAAAAGCGAGGAGAAGACCTCTCCACACGGCCAGTGAGGTCCTAA

Upstream 100 bases:

>100_bases
GATCGGATCAACGACGGTCAATACAGATAAACGATTCCTCACTCAAAAATCAGCTCAACCGCAAGCCGTCGGTTCACCCG
GCGGCTTTTTTGTAGGTTTC

Downstream 100 bases:

>100_bases
CTGGGCTGCTTCAGAACTGCCTCGCGCCCTGATAGCCACTCAGATCAGCCAGCTTATTGAGCGGTTTCACTCCGGATTCG
AGCTCCCCGTTAATTTCCCA

Product: L-aspartate oxidase

Products: NA

Alternate protein names: LASPO; Quinolinate synthase B [H]

Number of amino acids: Translated: 556; Mature: 555

Protein sequence:

>556_residues
MSISNPSTPIPNGPWDVVVIGAGAAGLMTCLELPAQLKVLLLNRNTSRHSSSRWAQGGIAAVTRPKDSAESHAEDTLKAG
AGLCDADAVRQLVEEAPHCVQRLQKLGMAFDREGDALATTLEAAHSHRRVLHVQDQTGRALVEALQEQVERRPNLLHRRG
VRATQLWIEHNRCYGVQVLDGTLLHWLSARAVVLATGGGGHLFANTTNPAQACGEGLALAWQAGATIEDLEFVQFHPTAL
KLEGAPCFLISEAVRGEGAVLVDGQGQSPVKELSAGDLAPRDQVSRSLIQTMHHQGVQHLGLDLTPIPSGQAEVRFPTIL
ERCRELGLDPLNQPIPVAPAAHYWMGGIATNLQAATNLPGLYAIGEVACTGVHGANRLAGNSLMECMVFARQMATIDLGS
LPRLGVHQRDSSPEPCCQQLHGTATAESLTRAIADLRELCWRTAGVDRSAKGMKQSLASIRKDLQHIGEQPLLKLVSHQP
QDICHHLDDTARRDLNLLLDLNHRQLTSSLMLEACLFRRESRGGHFRTDAPAEMPQWQRHSRQKRGEDLSTRPVRS

Sequences:

>Translated_556_residues
MSISNPSTPIPNGPWDVVVIGAGAAGLMTCLELPAQLKVLLLNRNTSRHSSSRWAQGGIAAVTRPKDSAESHAEDTLKAG
AGLCDADAVRQLVEEAPHCVQRLQKLGMAFDREGDALATTLEAAHSHRRVLHVQDQTGRALVEALQEQVERRPNLLHRRG
VRATQLWIEHNRCYGVQVLDGTLLHWLSARAVVLATGGGGHLFANTTNPAQACGEGLALAWQAGATIEDLEFVQFHPTAL
KLEGAPCFLISEAVRGEGAVLVDGQGQSPVKELSAGDLAPRDQVSRSLIQTMHHQGVQHLGLDLTPIPSGQAEVRFPTIL
ERCRELGLDPLNQPIPVAPAAHYWMGGIATNLQAATNLPGLYAIGEVACTGVHGANRLAGNSLMECMVFARQMATIDLGS
LPRLGVHQRDSSPEPCCQQLHGTATAESLTRAIADLRELCWRTAGVDRSAKGMKQSLASIRKDLQHIGEQPLLKLVSHQP
QDICHHLDDTARRDLNLLLDLNHRQLTSSLMLEACLFRRESRGGHFRTDAPAEMPQWQRHSRQKRGEDLSTRPVRS
>Mature_555_residues
SISNPSTPIPNGPWDVVVIGAGAAGLMTCLELPAQLKVLLLNRNTSRHSSSRWAQGGIAAVTRPKDSAESHAEDTLKAGA
GLCDADAVRQLVEEAPHCVQRLQKLGMAFDREGDALATTLEAAHSHRRVLHVQDQTGRALVEALQEQVERRPNLLHRRGV
RATQLWIEHNRCYGVQVLDGTLLHWLSARAVVLATGGGGHLFANTTNPAQACGEGLALAWQAGATIEDLEFVQFHPTALK
LEGAPCFLISEAVRGEGAVLVDGQGQSPVKELSAGDLAPRDQVSRSLIQTMHHQGVQHLGLDLTPIPSGQAEVRFPTILE
RCRELGLDPLNQPIPVAPAAHYWMGGIATNLQAATNLPGLYAIGEVACTGVHGANRLAGNSLMECMVFARQMATIDLGSL
PRLGVHQRDSSPEPCCQQLHGTATAESLTRAIADLRELCWRTAGVDRSAKGMKQSLASIRKDLQHIGEQPLLKLVSHQPQ
DICHHLDDTARRDLNLLLDLNHRQLTSSLMLEACLFRRESRGGHFRTDAPAEMPQWQRHSRQKRGEDLSTRPVRS

Specific function: Catalyzes the oxidation of L-aspartate to iminoaspartate [H]

COG id: COG0029

COG function: function code H; Aspartate oxidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=371, Percent_Identity=36.9272237196765, Blast_Score=190, Evalue=2e-48,
Organism=Escherichia coli, GI1788928, Length=537, Percent_Identity=36.1266294227188, Blast_Score=300, Evalue=1e-82,
Organism=Escherichia coli, GI1790597, Length=551, Percent_Identity=27.7676950998185, Blast_Score=187, Evalue=2e-48,
Organism=Escherichia coli, GI1786942, Length=585, Percent_Identity=30.4273504273504, Blast_Score=177, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI17550100, Length=534, Percent_Identity=32.3970037453184, Blast_Score=193, Evalue=2e-49,
Organism=Caenorhabditis elegans, GI17505833, Length=581, Percent_Identity=30.6368330464716, Blast_Score=189, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI71986328, Length=421, Percent_Identity=27.5534441805226, Blast_Score=96, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6322701, Length=577, Percent_Identity=31.0225303292894, Blast_Score=183, Evalue=6e-47,
Organism=Saccharomyces cerevisiae, GI6322416, Length=573, Percent_Identity=30.8900523560209, Blast_Score=175, Evalue=2e-44,
Organism=Drosophila melanogaster, GI17137288, Length=578, Percent_Identity=31.8339100346021, Blast_Score=202, Evalue=4e-52,
Organism=Drosophila melanogaster, GI24655642, Length=578, Percent_Identity=31.8339100346021, Blast_Score=202, Evalue=4e-52,
Organism=Drosophila melanogaster, GI24655647, Length=578, Percent_Identity=31.8339100346021, Blast_Score=202, Evalue=4e-52,
Organism=Drosophila melanogaster, GI24663005, Length=523, Percent_Identity=29.4455066921606, Blast_Score=165, Evalue=9e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112
- InterPro:   IPR005288 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: =1.4.3.16 [H]

Molecular weight: Translated: 60422; Mature: 60290

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSISNPSTPIPNGPWDVVVIGAGAAGLMTCLELPAQLKVLLLNRNTSRHSSSRWAQGGIA
CCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHCCHHEEEEEEECCCCCCCCCHHHCCCEE
AVTRPKDSAESHAEDTLKAGAGLCDADAVRQLVEEAPHCVQRLQKLGMAFDREGDALATT
EEECCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCHHHHH
LEAAHSHRRVLHVQDQTGRALVEALQEQVERRPNLLHRRGVRATQLWIEHNRCYGVQVLD
HHHHHCCCEEEEECCCCCHHHHHHHHHHHHHCHHHHHHCCCCHHHEEEECCCEEEEEEHH
GTLLHWLSARAVVLATGGGGHLFANTTNPAQACGEGLALAWQAGATIEDLEFVQFHPTAL
HHHHHHHCCCEEEEEECCCCEEEECCCCHHHHHCCCCEEEEECCCCHHHCHHEEECCCEE
KLEGAPCFLISEAVRGEGAVLVDGQGQSPVKELSAGDLAPRDQVSRSLIQTMHHQGVQHL
EECCCCEEEEHHHHCCCCEEEECCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHH
GLDLTPIPSGQAEVRFPTILERCRELGLDPLNQPIPVAPAAHYWMGGIATNLQAATNLPG
CCCCCCCCCCCCCEECHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHCCCCC
LYAIGEVACTGVHGANRLAGNSLMECMVFARQMATIDLGSLPRLGVHQRDSSPEPCCQQL
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHH
HGTATAESLTRAIADLRELCWRTAGVDRSAKGMKQSLASIRKDLQHIGEQPLLKLVSHQP
CCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCH
QDICHHLDDTARRDLNLLLDLNHRQLTSSLMLEACLFRRESRGGHFRTDAPAEMPQWQRH
HHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHH
SRQKRGEDLSTRPVRS
HHHHCCCCCCCCCCCC
>Mature Secondary Structure 
SISNPSTPIPNGPWDVVVIGAGAAGLMTCLELPAQLKVLLLNRNTSRHSSSRWAQGGIA
CCCCCCCCCCCCCCCEEEECCCHHHHHHHHHCCHHEEEEEEECCCCCCCCCHHHCCCEE
AVTRPKDSAESHAEDTLKAGAGLCDADAVRQLVEEAPHCVQRLQKLGMAFDREGDALATT
EEECCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCHHHHH
LEAAHSHRRVLHVQDQTGRALVEALQEQVERRPNLLHRRGVRATQLWIEHNRCYGVQVLD
HHHHHCCCEEEEECCCCCHHHHHHHHHHHHHCHHHHHHCCCCHHHEEEECCCEEEEEEHH
GTLLHWLSARAVVLATGGGGHLFANTTNPAQACGEGLALAWQAGATIEDLEFVQFHPTAL
HHHHHHHCCCEEEEEECCCCEEEECCCCHHHHHCCCCEEEEECCCCHHHCHHEEECCCEE
KLEGAPCFLISEAVRGEGAVLVDGQGQSPVKELSAGDLAPRDQVSRSLIQTMHHQGVQHL
EECCCCEEEEHHHHCCCCEEEECCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHH
GLDLTPIPSGQAEVRFPTILERCRELGLDPLNQPIPVAPAAHYWMGGIATNLQAATNLPG
CCCCCCCCCCCCCEECHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHCCCCC
LYAIGEVACTGVHGANRLAGNSLMECMVFARQMATIDLGSLPRLGVHQRDSSPEPCCQQL
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHH
HGTATAESLTRAIADLRELCWRTAGVDRSAKGMKQSLASIRKDLQHIGEQPLLKLVSHQP
CCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCH
QDICHHLDDTARRDLNLLLDLNHRQLTSSLMLEACLFRRESRGGHFRTDAPAEMPQWQRH
HHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHH
SRQKRGEDLSTRPVRS
HHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]