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The map label for this gene is rimO [H]

Identifier: 33862448

GI number: 33862448

Start: 197762

End: 199180

Strand: Reverse

Name: rimO [H]

Synonym: PMT0175

Alternate gene names: 33862448

Gene position: 199180-197762 (Counterclockwise)

Preceding gene: 33862450

Following gene: 33862447

Centisome position: 8.26

GC content: 57.15

Gene sequence:

>1419_bases
ATGACCAAGCCTGCGTTGCGATCCGACATACCAATGAAGCCCACAGTCCACAAACAAGAAAAACCATCGGTGGCCTTTGC
CCATTTGGGCTGTGAGAAAAATCGCGTTGACACCGAACACATGCTGGGACTGCTTACAGAAGCGGGCTATAGCGTCAGCA
GCGATGAAAACGATGCCGCCGTCGTGGTGGTCAACACCTGCAGCTTCATCCAGGACGCGCGGGAGGAATCGGTGCGGACG
TTGATTGGTCTAGCAGAACAGGGCAAGGAACTGATCATCGCCGGATGCCTTGCCCAGCATTTTCAAGAGGAACTGCTCGA
GTCAATCCCGGAGGCAAAAGCAATCGTCGGCACCGGTGACTATCAGCACATCGTCGATGTGCTGAAGCGAGTAGAAGCGG
GTGAACGGGTGAACCATGTCAGCGAATTCCCCACGTTTGTGGGCGATGAGACTCTCCCCCGGCAGCGCACCACTGACCAG
GCTGTGGCTTATCTGAAAGTTGCTGAGGGATGTGATTACCGCTGTGCCTTCTGCATCATCCCAAAGCTGCGTGGCGACCA
GCGCAGCCGACCGGTGGAGTCGATTGTGACAGAGGCGCACCAACTGGCTGAGCAGGGGGTGCAGGAACTAATCCTGATCA
GCCAAATCACCACCAACTATGGCCTTGACCTCTATGGCAAGCCCAAATTTGCAGAGCTGCTACAGGCGCTGGGGGAGGTA
GACATCCCCTGGGTGAGGGTGCATTACGCCTACCCCACTGGCCTCACTCCAGAGGTGCTGGCGGCCTATCGGGAGGTGCC
AAATGTGCTTCGCTATCTCGACCTTCCACTGCAACACAGCCATCCAGAGGTGCTACGGGCTATGAACCGTCCCTGGCAAA
CGGATGTGAACGAACGGCTCTTGGATCGGATTCGTGAGCAGCTACCAGACGCAGTCCTGCGTACCACGCTCATCGTCGGC
TTCCCAGGGGAAACTGAAGATCACTTCAATCACCTCGCCGCCTTCATCGAACGGCAGCGTTTTGATCACGTTGGCGTGTT
CACCTTTTCTCCAGAAGATGGAACCGCCGCAGCCGATCTACCTGACAGGGTAGACCCGTCAATCGCCGCAGCCCGCAAGG
ATCGATTGATGGCGCTTCAGCAACCCATCTCAGCGGAAAGGAACCAAAGATGGGTTGGACGCACCATCGATGTGCTGATC
GAGCAGCACAACCCCGAAACCGGAGCGATGATTGGCCGCTGCGATCGTTTCGCTCCGGAGGTCGATGGCGAGGTGTTGGT
ACTGCCTAGTGAGAAAGGTCTGCAGGCCAGTCCAGGAACGATGGTTCCTGTCTTCATTACCGGCTCTGATGTCTATGACC
TCACCGGCCAGCTGGTAGACACCAACGCCATGGCGGTCACAGCCCAGACGTCTCAGTGA

Upstream 100 bases:

>100_bases
CACAGATCTAAGAATTGTGGCAGCTCTGGAAGAAGCCAGCCTCTCTTTAGGGTGGCGCCTAGCGGGAACTGCCTAGGCAG
CCCAGAAATCAACCCTTTCA

Downstream 100 bases:

>100_bases
GTCCATTCCCCTGTTTGCGAGCCCATCAACGCAACATCTTTCTGACAGCCTCTGGCATCAGTACTGCAGGCTCGTTCGCT
GGAATGACTGCAAAAGGCTG

Product: Fe-S oxidoreductase

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO [H]

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MTKPALRSDIPMKPTVHKQEKPSVAFAHLGCEKNRVDTEHMLGLLTEAGYSVSSDENDAAVVVVNTCSFIQDAREESVRT
LIGLAEQGKELIIAGCLAQHFQEELLESIPEAKAIVGTGDYQHIVDVLKRVEAGERVNHVSEFPTFVGDETLPRQRTTDQ
AVAYLKVAEGCDYRCAFCIIPKLRGDQRSRPVESIVTEAHQLAEQGVQELILISQITTNYGLDLYGKPKFAELLQALGEV
DIPWVRVHYAYPTGLTPEVLAAYREVPNVLRYLDLPLQHSHPEVLRAMNRPWQTDVNERLLDRIREQLPDAVLRTTLIVG
FPGETEDHFNHLAAFIERQRFDHVGVFTFSPEDGTAAADLPDRVDPSIAAARKDRLMALQQPISAERNQRWVGRTIDVLI
EQHNPETGAMIGRCDRFAPEVDGEVLVLPSEKGLQASPGTMVPVFITGSDVYDLTGQLVDTNAMAVTAQTSQ

Sequences:

>Translated_472_residues
MTKPALRSDIPMKPTVHKQEKPSVAFAHLGCEKNRVDTEHMLGLLTEAGYSVSSDENDAAVVVVNTCSFIQDAREESVRT
LIGLAEQGKELIIAGCLAQHFQEELLESIPEAKAIVGTGDYQHIVDVLKRVEAGERVNHVSEFPTFVGDETLPRQRTTDQ
AVAYLKVAEGCDYRCAFCIIPKLRGDQRSRPVESIVTEAHQLAEQGVQELILISQITTNYGLDLYGKPKFAELLQALGEV
DIPWVRVHYAYPTGLTPEVLAAYREVPNVLRYLDLPLQHSHPEVLRAMNRPWQTDVNERLLDRIREQLPDAVLRTTLIVG
FPGETEDHFNHLAAFIERQRFDHVGVFTFSPEDGTAAADLPDRVDPSIAAARKDRLMALQQPISAERNQRWVGRTIDVLI
EQHNPETGAMIGRCDRFAPEVDGEVLVLPSEKGLQASPGTMVPVFITGSDVYDLTGQLVDTNAMAVTAQTSQ
>Mature_471_residues
TKPALRSDIPMKPTVHKQEKPSVAFAHLGCEKNRVDTEHMLGLLTEAGYSVSSDENDAAVVVVNTCSFIQDAREESVRTL
IGLAEQGKELIIAGCLAQHFQEELLESIPEAKAIVGTGDYQHIVDVLKRVEAGERVNHVSEFPTFVGDETLPRQRTTDQA
VAYLKVAEGCDYRCAFCIIPKLRGDQRSRPVESIVTEAHQLAEQGVQELILISQITTNYGLDLYGKPKFAELLQALGEVD
IPWVRVHYAYPTGLTPEVLAAYREVPNVLRYLDLPLQHSHPEVLRAMNRPWQTDVNERLLDRIREQLPDAVLRTTLIVGF
PGETEDHFNHLAAFIERQRFDHVGVFTFSPEDGTAAADLPDRVDPSIAAARKDRLMALQQPISAERNQRWVGRTIDVLIE
QHNPETGAMIGRCDRFAPEVDGEVLVLPSEKGLQASPGTMVPVFITGSDVYDLTGQLVDTNAMAVTAQTSQ

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12 [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872782, Length=493, Percent_Identity=27.5862068965517, Blast_Score=166, Evalue=6e-41,
Organism=Homo sapiens, GI28872784, Length=493, Percent_Identity=27.5862068965517, Blast_Score=165, Evalue=7e-41,
Organism=Homo sapiens, GI93277076, Length=343, Percent_Identity=27.9883381924198, Blast_Score=131, Evalue=2e-30,
Organism=Escherichia coli, GI1787057, Length=452, Percent_Identity=38.716814159292, Blast_Score=286, Evalue=1e-78,
Organism=Escherichia coli, GI1786882, Length=385, Percent_Identity=30.3896103896104, Blast_Score=164, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI17553146, Length=477, Percent_Identity=25.7861635220126, Blast_Score=157, Evalue=8e-39,
Organism=Caenorhabditis elegans, GI71996771, Length=321, Percent_Identity=25.8566978193146, Blast_Score=85, Evalue=9e-17,
Organism=Drosophila melanogaster, GI21356207, Length=431, Percent_Identity=28.3062645011601, Blast_Score=170, Evalue=2e-42,
Organism=Drosophila melanogaster, GI19922432, Length=467, Percent_Identity=28.6937901498929, Blast_Score=152, Evalue=6e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 52400; Mature: 52269

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKPALRSDIPMKPTVHKQEKPSVAFAHLGCEKNRVDTEHMLGLLTEAGYSVSSDENDAA
CCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCE
VVVVNTCSFIQDAREESVRTLIGLAEQGKELIIAGCLAQHFQEELLESIPEAKAIVGTGD
EEEECCHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHCCCCCEEECCCC
YQHIVDVLKRVEAGERVNHVSEFPTFVGDETLPRQRTTDQAVAYLKVAEGCDYRCAFCII
HHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEE
PKLRGDQRSRPVESIVTEAHQLAEQGVQELILISQITTNYGLDLYGKPKFAELLQALGEV
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCC
DIPWVRVHYAYPTGLTPEVLAAYREVPNVLRYLDLPLQHSHPEVLRAMNRPWQTDVNERL
CCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCHHHHH
LDRIREQLPDAVLRTTLIVGFPGETEDHFNHLAAFIERQRFDHVGVFTFSPEDGTAAADL
HHHHHHHCCHHHHHHEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCC
PDRVDPSIAAARKDRLMALQQPISAERNQRWVGRTIDVLIEQHNPETGAMIGRCDRFAPE
CCCCCCHHHHHHHHHHHHHHCCCCHHHCCHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCC
VDGEVLVLPSEKGLQASPGTMVPVFITGSDVYDLTGQLVDTNAMAVTAQTSQ
CCCCEEEEECCCCCCCCCCCEEEEEEECCCHHHCCCCEEECCEEEEEEECCC
>Mature Secondary Structure 
TKPALRSDIPMKPTVHKQEKPSVAFAHLGCEKNRVDTEHMLGLLTEAGYSVSSDENDAA
CCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCE
VVVVNTCSFIQDAREESVRTLIGLAEQGKELIIAGCLAQHFQEELLESIPEAKAIVGTGD
EEEECCHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHCCCCCEEECCCC
YQHIVDVLKRVEAGERVNHVSEFPTFVGDETLPRQRTTDQAVAYLKVAEGCDYRCAFCII
HHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEE
PKLRGDQRSRPVESIVTEAHQLAEQGVQELILISQITTNYGLDLYGKPKFAELLQALGEV
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCC
DIPWVRVHYAYPTGLTPEVLAAYREVPNVLRYLDLPLQHSHPEVLRAMNRPWQTDVNERL
CCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCHHHHH
LDRIREQLPDAVLRTTLIVGFPGETEDHFNHLAAFIERQRFDHVGVFTFSPEDGTAAADL
HHHHHHHCCHHHHHHEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCC
PDRVDPSIAAARKDRLMALQQPISAERNQRWVGRTIDVLIEQHNPETGAMIGRCDRFAPE
CCCCCCHHHHHHHHHHHHHHCCCCHHHCCHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCC
VDGEVLVLPSEKGLQASPGTMVPVFITGSDVYDLTGQLVDTNAMAVTAQTSQ
CCCCEEEEECCCCCCCCCCCEEEEEEECCCHHHCCCCEEECCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA