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The map label for this gene is lysC [H]

Identifier: 33862346

GI number: 33862346

Start: 75605

End: 77392

Strand: Reverse

Name: lysC [H]

Synonym: PMT0073

Alternate gene names: 33862346

Gene position: 77392-75605 (Counterclockwise)

Preceding gene: 33862347

Following gene: 33862341

Centisome position: 3.21

GC content: 57.1

Gene sequence:

>1788_bases
ATGGCGTTGCTGGTGCAAAAATTCGGCGGATCATCCGTCGGCAACGTCGAGCGCATCCAAGCCGTCGCTGAGCGGATTGC
GACCAGCAAAGATCTCGGCCACGACCTAGTGATTGTGGTATCCGCTATGGGCCAAACCACTGATGAGCTCACAGCCCTAG
CGAAAGTGATCAGCAGCAATCCCCCTCAACGGGAAATGGACATGCTGCTAGCCACCGGAGAACAGGTTTCGATCTCCTTG
CTTTCAATGGCACTGCATGAACTGGGTATACCAGCCATTTCGATGACTGGAGCCCAAGTGGGCATCGTGACAGAGTCTGC
CTATGGCCGCGCTCGAATCCTAGACGTCCGCACTGAGCGCCTGCAATCCCTGTTGAGCACTGGCAAGGTGGTAGTCGTAG
CAGGCTTCCAGGGCACAAGCCTGAGCAGCGTTGGCACCGCAGAGATCACCACCCTCGGCCGCGGTGGTTCAGATACATCT
GCAGTTGCCATAGCAGCCGCCCTAGGAGCAGATGCCTGCGAGATCTACACCGATGTACCAGGAGTGCTCACAAGTGATCC
ACGTCAGGTCAGCGATGCCCAACTGATGCAAGAAGTGAGTTGCGACGAGATGCTTGAGCTGGCCAGTCTGGGCGCCTCCG
TGTTGCACCCCCGTGCAGTCGAGATCGCCCGCAACTTTGGAGTCGCCCTGGTCGTGCGTTCTAGCTGGAGCAACGAAGCC
GGAACCAAGCTCACAAGCAAATCAAAGCGCCCCATCGGTCGAGAAGGTCTAGAGCTTGGTCGGCCTGTGGATGGCGTTGA
ACTTGCCGAAGGTCAAGCGGTGATCGCCCTCTCTCACGTGCCAGATCAACCGGGTGTCGCAGCCACACTTTTTGAAAACC
TCTCTAGCGATGGCGTGAATGTGGACCTAATCATCCAATCAACCCATGAAGGCAACAGCAATGACATTGCCTTCACAGTG
GCCGAAGCCGACTTAGCGAAAGCCCACTCACTTTGCGAAGGGCTGCTTAAAAGCGTCGGTGGCCAACTCTCAGCTGAAGC
CGGAATGAGCAAACTAAGCATCAGCGGAGCAGGAATCATGGGACGCCCCGGTATCGCCGCAAGCCTTTTCGACACGATCT
CACGGGCAGGAATCAACCTACGGCTGATCGCCACCAGTGAGGTGAAAGTCAGCTGCGTCATCGATGCAACGCTGGGAAGC
AAAGCCCTGCGTGCAGTCAGCACAGCCTTTGAACTCAGCGACAGCCAAGTACGCATCAATCCTTTGGGCAACGGCAAAGG
TGAACCAGAAGTTCGAGGGGTAGCACTCGATCAAGGTCAGGCCCAGCTAAGTGTGCGAGGGGTGCCGAATCGGCCTGGGA
CAGCAGCAGCTCTTTGTTCAGCGATGGCAGAGGCAGGAATCAGCCTCGATGCGATTGTGCAATCGGAGCGACAACATGAC
GATGGAAGCCGGGATATCAGCTTCACCCTCAAGCGCGATGAGCGAGAGCAAGCCAATAAGACTCTTGCCCCCCTACTTGC
GCAATGGCCAGGAGCCAATCTGGAAGACGGTCCTGCAATCGCCCGGGTGAGCGCAGTGGGTGCAGGCATGCCCGCCACCG
CAGGTACAGCAGGGCGGATGTTCCGAGCTCTCGCAGATGCAAAGATCAATATCGCCATGATCGCCACCAGTGAAATCCGC
ACAAGTTGCGTGGTGGCTGAACCCGATGGGGTCAAGGCGCTGCAATCAGTGCATAAAGGCTTTGGGCTGGGTGAAGAAGC
TGATCATCCAGTGCAAAGCAACTCGTAA

Upstream 100 bases:

>100_bases
AGGTGCAATCCCTACTGATGCCTTCCGCGACGGGTTACTCAGCACTCCCTAATCCAGTGACGACACGCTGAAATAATCAG
AACGTGTGTCAAAGGCATGG

Downstream 100 bases:

>100_bases
TGGCTGAGAAGTTCAGCTAGGCAATCATTCAGCAGAAAGACAGACAGTTTTGATGGCTTGATTCAGCTCCAAATAAACGT
TGCGCCAACCTTCCAGTCTT

Product: aspartate kinase

Products: NA

Alternate protein names: Aspartate kinase 2; Aspartokinase II [H]

Number of amino acids: Translated: 595; Mature: 594

Protein sequence:

>595_residues
MALLVQKFGGSSVGNVERIQAVAERIATSKDLGHDLVIVVSAMGQTTDELTALAKVISSNPPQREMDMLLATGEQVSISL
LSMALHELGIPAISMTGAQVGIVTESAYGRARILDVRTERLQSLLSTGKVVVVAGFQGTSLSSVGTAEITTLGRGGSDTS
AVAIAAALGADACEIYTDVPGVLTSDPRQVSDAQLMQEVSCDEMLELASLGASVLHPRAVEIARNFGVALVVRSSWSNEA
GTKLTSKSKRPIGREGLELGRPVDGVELAEGQAVIALSHVPDQPGVAATLFENLSSDGVNVDLIIQSTHEGNSNDIAFTV
AEADLAKAHSLCEGLLKSVGGQLSAEAGMSKLSISGAGIMGRPGIAASLFDTISRAGINLRLIATSEVKVSCVIDATLGS
KALRAVSTAFELSDSQVRINPLGNGKGEPEVRGVALDQGQAQLSVRGVPNRPGTAAALCSAMAEAGISLDAIVQSERQHD
DGSRDISFTLKRDEREQANKTLAPLLAQWPGANLEDGPAIARVSAVGAGMPATAGTAGRMFRALADAKINIAMIATSEIR
TSCVVAEPDGVKALQSVHKGFGLGEEADHPVQSNS

Sequences:

>Translated_595_residues
MALLVQKFGGSSVGNVERIQAVAERIATSKDLGHDLVIVVSAMGQTTDELTALAKVISSNPPQREMDMLLATGEQVSISL
LSMALHELGIPAISMTGAQVGIVTESAYGRARILDVRTERLQSLLSTGKVVVVAGFQGTSLSSVGTAEITTLGRGGSDTS
AVAIAAALGADACEIYTDVPGVLTSDPRQVSDAQLMQEVSCDEMLELASLGASVLHPRAVEIARNFGVALVVRSSWSNEA
GTKLTSKSKRPIGREGLELGRPVDGVELAEGQAVIALSHVPDQPGVAATLFENLSSDGVNVDLIIQSTHEGNSNDIAFTV
AEADLAKAHSLCEGLLKSVGGQLSAEAGMSKLSISGAGIMGRPGIAASLFDTISRAGINLRLIATSEVKVSCVIDATLGS
KALRAVSTAFELSDSQVRINPLGNGKGEPEVRGVALDQGQAQLSVRGVPNRPGTAAALCSAMAEAGISLDAIVQSERQHD
DGSRDISFTLKRDEREQANKTLAPLLAQWPGANLEDGPAIARVSAVGAGMPATAGTAGRMFRALADAKINIAMIATSEIR
TSCVVAEPDGVKALQSVHKGFGLGEEADHPVQSNS
>Mature_594_residues
ALLVQKFGGSSVGNVERIQAVAERIATSKDLGHDLVIVVSAMGQTTDELTALAKVISSNPPQREMDMLLATGEQVSISLL
SMALHELGIPAISMTGAQVGIVTESAYGRARILDVRTERLQSLLSTGKVVVVAGFQGTSLSSVGTAEITTLGRGGSDTSA
VAIAAALGADACEIYTDVPGVLTSDPRQVSDAQLMQEVSCDEMLELASLGASVLHPRAVEIARNFGVALVVRSSWSNEAG
TKLTSKSKRPIGREGLELGRPVDGVELAEGQAVIALSHVPDQPGVAATLFENLSSDGVNVDLIIQSTHEGNSNDIAFTVA
EADLAKAHSLCEGLLKSVGGQLSAEAGMSKLSISGAGIMGRPGIAASLFDTISRAGINLRLIATSEVKVSCVIDATLGSK
ALRAVSTAFELSDSQVRINPLGNGKGEPEVRGVALDQGQAQLSVRGVPNRPGTAAALCSAMAEAGISLDAIVQSERQHDD
GSRDISFTLKRDEREQANKTLAPLLAQWPGANLEDGPAIARVSAVGAGMPATAGTAGRMFRALADAKINIAMIATSEIRT
SCVVAEPDGVKALQSVHKGFGLGEEADHPVQSNS

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ACT domains [H]

Homologues:

Organism=Escherichia coli, GI1786183, Length=383, Percent_Identity=31.5926892950392, Blast_Score=134, Evalue=2e-32,
Organism=Escherichia coli, GI1790376, Length=289, Percent_Identity=30.4498269896194, Blast_Score=86, Evalue=8e-18,
Organism=Escherichia coli, GI1790455, Length=181, Percent_Identity=35.9116022099448, Blast_Score=78, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6320893, Length=377, Percent_Identity=24.9336870026525, Blast_Score=95, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR001048
- InterPro:   IPR005260
- InterPro:   IPR001341
- InterPro:   IPR018042 [H]

Pfam domain/function: PF00696 AA_kinase; PF01842 ACT [H]

EC number: =2.7.2.4 [H]

Molecular weight: Translated: 61387; Mature: 61256

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS00324 ASPARTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALLVQKFGGSSVGNVERIQAVAERIATSKDLGHDLVIVVSAMGQTTDELTALAKVISSN
CEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEHHCCCHHHHHHHHHHHHCC
PPQREMDMLLATGEQVSISLLSMALHELGIPAISMTGAQVGIVTESAYGRARILDVRTER
CCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEECCCEEEEEECCCCCCEEEEHHHHHH
LQSLLSTGKVVVVAGFQGTSLSSVGTAEITTLGRGGSDTSAVAIAAALGADACEIYTDVP
HHHHHCCCCEEEEECCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCHHHHHHCCC
GVLTSDPRQVSDAQLMQEVSCDEMLELASLGASVLHPRAVEIARNFGVALVVRSSWSNEA
CCCCCCCCHHHHHHHHHHCCHHHHHHHHHCCHHHHCCHHHHHHHCCCEEEEEECCCCCCC
GTKLTSKSKRPIGREGLELGRPVDGVELAEGQAVIALSHVPDQPGVAATLFENLSSDGVN
CCCCCCCCCCCCCCCCHHHCCCCCCCEECCCCEEEEEECCCCCCCHHHHHHHHHCCCCCE
VDLIIQSTHEGNSNDIAFTVAEADLAKAHSLCEGLLKSVGGQLSAEAGMSKLSISGAGIM
EEEEEEECCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCCHHCCCCEEEECCCCCC
GRPGIAASLFDTISRAGINLRLIATSEVKVSCVIDATLGSKALRAVSTAFELSDSQVRIN
CCCCHHHHHHHHHHHCCCEEEEEEECCEEEEEEEECCCCHHHHHHHHHHHCCCCCEEEEE
PLGNGKGEPEVRGVALDQGQAQLSVRGVPNRPGTAAALCSAMAEAGISLDAIVQSERQHD
ECCCCCCCCCEEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCC
DGSRDISFTLKRDEREQANKTLAPLLAQWPGANLEDGPAIARVSAVGAGMPATAGTAGRM
CCCCCEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCHHEEHHHHCCCCCCCCCHHHHH
FRALADAKINIAMIATSEIRTSCVVAEPDGVKALQSVHKGFGLGEEADHPVQSNS
HHHHHCCEEEEEEEEECCCCCEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
ALLVQKFGGSSVGNVERIQAVAERIATSKDLGHDLVIVVSAMGQTTDELTALAKVISSN
EEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEHHCCCHHHHHHHHHHHHCC
PPQREMDMLLATGEQVSISLLSMALHELGIPAISMTGAQVGIVTESAYGRARILDVRTER
CCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEECCCEEEEEECCCCCCEEEEHHHHHH
LQSLLSTGKVVVVAGFQGTSLSSVGTAEITTLGRGGSDTSAVAIAAALGADACEIYTDVP
HHHHHCCCCEEEEECCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCHHHHHHCCC
GVLTSDPRQVSDAQLMQEVSCDEMLELASLGASVLHPRAVEIARNFGVALVVRSSWSNEA
CCCCCCCCHHHHHHHHHHCCHHHHHHHHHCCHHHHCCHHHHHHHCCCEEEEEECCCCCCC
GTKLTSKSKRPIGREGLELGRPVDGVELAEGQAVIALSHVPDQPGVAATLFENLSSDGVN
CCCCCCCCCCCCCCCCHHHCCCCCCCEECCCCEEEEEECCCCCCCHHHHHHHHHCCCCCE
VDLIIQSTHEGNSNDIAFTVAEADLAKAHSLCEGLLKSVGGQLSAEAGMSKLSISGAGIM
EEEEEEECCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCCHHCCCCEEEECCCCCC
GRPGIAASLFDTISRAGINLRLIATSEVKVSCVIDATLGSKALRAVSTAFELSDSQVRIN
CCCCHHHHHHHHHHHCCCEEEEEEECCEEEEEEEECCCCHHHHHHHHHHHCCCCCEEEEE
PLGNGKGEPEVRGVALDQGQAQLSVRGVPNRPGTAAALCSAMAEAGISLDAIVQSERQHD
ECCCCCCCCCEEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCC
DGSRDISFTLKRDEREQANKTLAPLLAQWPGANLEDGPAIARVSAVGAGMPATAGTAGRM
CCCCCEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCHHEEHHHHCCCCCCCCCHHHHH
FRALADAKINIAMIATSEIRTSCVVAEPDGVKALQSVHKGFGLGEEADHPVQSNS
HHHHHCCEEEEEEEEECCCCCEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1444390 [H]