Click here to switch to the map view.

The map label for this gene is tilS

Identifier: 33862341

GI number: 33862341

Start: 70488

End: 71519

Strand: Reverse

Name: tilS

Synonym: PMT0068

Alternate gene names: 33862341

Gene position: 71519-70488 (Counterclockwise)

Preceding gene: 33862346

Following gene: 33862340

Centisome position: 2.97

GC content: 53.88

Gene sequence:

>1032_bases
ATGGCCTCATCCAGCTCAACGCAACTGCCCTGGAGCGCCTGGCACGCGCGTCTCCATCACAAACTGCTAGCAAATCCAGA
TCTACTGCCAGCAAAAGCCTCACTCTTGATCGCCGTGTCCGGCGGTCAAGATTCAATGGCCCTGCTTGGTCTATTGATCG
ATCTGCAACGCAAACATGGCTGGTCTTTAGAGGTATGGCATGGCGACCACAACTGGCATAAACAATCAGCAACGATCGCA
ACTGAACTCAAAAATTGGTGTGAATCACACAACTTGAGCTTCTGCTCTGATCAAGCCAAACCTGGACAAACCAACAACGA
AGCCACAGCGCGACATTGGCGATATGAACAACTGACACTTCACGCAGAACGTTTGTCATCCAACAACCCCAACCATCCAT
GCCGGTATGTGCTCACAGGCCACACCAGTTCTGATCGAGCTGAAACCTTGCTGCTCAACCTTGCCCGAGGTACCGACCTC
GCGGGACTGAGCAGCATGCGCCAATGCAGGCCCATCAGCAAAGACCATCCTCACAAAAACGTGCAGCTCATACGCCCTTT
GCTGGGGTTCTCGCGTGAGGACACCGCCTTGATTTGCAGCGAGCTGGGCTTACCTATTTGGCTTGATCCTGCCAATTCCA
ATCCAGACTTCAGCCGCAACCGAGTCCGCCAAGAAGTACTCCCAGTACTGGAATCTCTCCATCCTGGCTGCAGCTTGCGC
ATGGCCGCTCTCGCCGAAAGGCTCAACCATCACCATGCGGACCAGCAAGCGATCGCCATGCTGGCTCTAAACAACCTCAG
CAAGCAAAACGGCCTTTGTCGTAAGGACCTTGCCCAACTACCGATCACAGCAAGAACAACTCTGTTGGCCTATTGGCTCA
AGCGATCAGGAGCACCAAGTCTGCCAGCAGTCCAACTTGAACAGATCAGCCAAAGCATTGCCCCCGGCAAACCACCTGGA
TCTCTACACCTCGCGCAGGGCTGGAAAGTCCAATGGCAGCGAAACTCGGTACAACTAGAACATCACGACTGA

Upstream 100 bases:

>100_bases
TGAGCGAGACGGGTCATTTCATCAACGATCTATAGCTCCATACTCCCATTTGCCAGGCTCCTGCACGGCAAACTGGCCTC
AGCTGAATGACCACTGCGCC

Downstream 100 bases:

>100_bases
CTCCGGTGGACGGTGACTTCCAACAGCAGTACCTGGCCGCCGAGAAGGCCTATGGGGCAGGAAATTACGAAGCAGCTCAA
TCAATCTGTGAAGGCCTGCT

Product: hypothetical protein

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase

Number of amino acids: Translated: 343; Mature: 342

Protein sequence:

>343_residues
MASSSSTQLPWSAWHARLHHKLLANPDLLPAKASLLIAVSGGQDSMALLGLLIDLQRKHGWSLEVWHGDHNWHKQSATIA
TELKNWCESHNLSFCSDQAKPGQTNNEATARHWRYEQLTLHAERLSSNNPNHPCRYVLTGHTSSDRAETLLLNLARGTDL
AGLSSMRQCRPISKDHPHKNVQLIRPLLGFSREDTALICSELGLPIWLDPANSNPDFSRNRVRQEVLPVLESLHPGCSLR
MAALAERLNHHHADQQAIAMLALNNLSKQNGLCRKDLAQLPITARTTLLAYWLKRSGAPSLPAVQLEQISQSIAPGKPPG
SLHLAQGWKVQWQRNSVQLEHHD

Sequences:

>Translated_343_residues
MASSSSTQLPWSAWHARLHHKLLANPDLLPAKASLLIAVSGGQDSMALLGLLIDLQRKHGWSLEVWHGDHNWHKQSATIA
TELKNWCESHNLSFCSDQAKPGQTNNEATARHWRYEQLTLHAERLSSNNPNHPCRYVLTGHTSSDRAETLLLNLARGTDL
AGLSSMRQCRPISKDHPHKNVQLIRPLLGFSREDTALICSELGLPIWLDPANSNPDFSRNRVRQEVLPVLESLHPGCSLR
MAALAERLNHHHADQQAIAMLALNNLSKQNGLCRKDLAQLPITARTTLLAYWLKRSGAPSLPAVQLEQISQSIAPGKPPG
SLHLAQGWKVQWQRNSVQLEHHD
>Mature_342_residues
ASSSSTQLPWSAWHARLHHKLLANPDLLPAKASLLIAVSGGQDSMALLGLLIDLQRKHGWSLEVWHGDHNWHKQSATIAT
ELKNWCESHNLSFCSDQAKPGQTNNEATARHWRYEQLTLHAERLSSNNPNHPCRYVLTGHTSSDRAETLLLNLARGTDLA
GLSSMRQCRPISKDHPHKNVQLIRPLLGFSREDTALICSELGLPIWLDPANSNPDFSRNRVRQEVLPVLESLHPGCSLRM
AALAERLNHHHADQQAIAMLALNNLSKQNGLCRKDLAQLPITARTTLLAYWLKRSGAPSLPAVQLEQISQSIAPGKPPGS
LHLAQGWKVQWQRNSVQLEHHD

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family

Homologues:

Organism=Escherichia coli, GI1786386, Length=294, Percent_Identity=27.2108843537415, Blast_Score=68, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TILS_PROMM (Q7V987)

Other databases:

- EMBL:   BX548175
- RefSeq:   NP_893901.1
- ProteinModelPortal:   Q7V987
- SMR:   Q7V987
- STRING:   Q7V987
- GeneID:   1727724
- GenomeReviews:   BX548175_GR
- KEGG:   pmt:PMT0068
- NMPDR:   fig|74547.1.peg.68
- eggNOG:   COG0037
- HOGENOM:   HBG692699
- OMA:   LIFSRED
- ProtClustDB:   CLSK646818
- BioCyc:   PMAR74547:PMT0068-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_01161
- InterPro:   IPR012094
- InterPro:   IPR012795
- InterPro:   IPR015262
- InterPro:   IPR014729
- InterPro:   IPR011063
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11807:SF2
- TIGRFAMs:   TIGR02432

Pfam domain/function: PF01171 ATP_bind_3; PF09179 DUF1946

EC number: NA

Molecular weight: Translated: 38260; Mature: 38129

Theoretical pI: Translated: 8.91; Mature: 8.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASSSSTQLPWSAWHARLHHKLLANPDLLPAKASLLIAVSGGQDSMALLGLLIDLQRKHG
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHCCC
WSLEVWHGDHNWHKQSATIATELKNWCESHNLSFCSDQAKPGQTNNEATARHWRYEQLTL
CEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCHHHHHHHHHHHHH
HAERLSSNNPNHPCRYVLTGHTSSDRAETLLLNLARGTDLAGLSSMRQCRPISKDHPHKN
HHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCH
VQLIRPLLGFSREDTALICSELGLPIWLDPANSNPDFSRNRVRQEVLPVLESLHPGCSLR
HHHHHHHHCCCCCCHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHH
MAALAERLNHHHADQQAIAMLALNNLSKQNGLCRKDLAQLPITARTTLLAYWLKRSGAPS
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHCCCCC
LPAVQLEQISQSIAPGKPPGSLHLAQGWKVQWQRNSVQLEHHD
CCHHHHHHHHHHHCCCCCCCCEEECCCCEEEEECCCEEEECCC
>Mature Secondary Structure 
ASSSSTQLPWSAWHARLHHKLLANPDLLPAKASLLIAVSGGQDSMALLGLLIDLQRKHG
CCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHCCC
WSLEVWHGDHNWHKQSATIATELKNWCESHNLSFCSDQAKPGQTNNEATARHWRYEQLTL
CEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCHHHHHHHHHHHHH
HAERLSSNNPNHPCRYVLTGHTSSDRAETLLLNLARGTDLAGLSSMRQCRPISKDHPHKN
HHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCH
VQLIRPLLGFSREDTALICSELGLPIWLDPANSNPDFSRNRVRQEVLPVLESLHPGCSLR
HHHHHHHHCCCCCCHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHH
MAALAERLNHHHADQQAIAMLALNNLSKQNGLCRKDLAQLPITARTTLLAYWLKRSGAPS
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHCCCCC
LPAVQLEQISQSIAPGKPPGSLHLAQGWKVQWQRNSVQLEHHD
CCHHHHHHHHHHHCCCCCCCCEEECCCCEEEEECCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12917642