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The map label for this gene is tig [H]

Identifier: 33862336

GI number: 33862336

Start: 63778

End: 65217

Strand: Reverse

Name: tig [H]

Synonym: PMT0063

Alternate gene names: 33862336

Gene position: 65217-63778 (Counterclockwise)

Preceding gene: 33862340

Following gene: 33862335

Centisome position: 2.71

GC content: 51.94

Gene sequence:

>1440_bases
ATGAGTGCCACAGCACTACAGGTCAAAACCACGCAGAAACCCAATAGTCGGCTTGCTGTCGAAGTAGCGGTACCAGCAGA
GCGCTGCCAGGCCAACTATGAGGCCGCTGTCACCCGCTTAAGCCGCACCACCAATCTTCCAGGCTTCCGCAAAGGGAAAG
TTCCGCGTGCCGTTCTGCTGCAACAGATCGGCCCGGTAAGAATCCGTGCAACAGCCTTGGAATCGCTCGTTGATGCTGTC
TGGCGTGAAGTCCTCGAACAAGAATCAATCGAGCCACTTTGCGAACCCGAACTCAGTGGCGGCTTTGACGCTCTTCTTGA
AAGCTTTCAGCCCAGCGAGGCACTGACCCTCACCCTTGAGACAGACGTCACCCCAACCCCAAAACTTAAGGCCACCAAAG
GGCTACAAGCCGAAGCAGAAGTGGTGACTTTCGATCCCAGCAAGGTCGATGAATTGATCGAACAATCCCGCAAACAACTA
GCCACCCTTGTACCCGTCGAAAGTCGCCCAGCAGCAATCGGCGATATCGCTGTGGTTAGTTTCAGCGGCACCTACGACGA
CGACGGCAGCGCAATCGAAGGCGGAAGTAGTGACTCCATGGATGTCGATCTTGAAGATGGACAAATGATCCCCGGCTTCG
TGGAAGGGATTATCGGTATGAACTTGGGCGATGAGAAAACTGTTGATTGCCACTTCCCAGACGATTACTCGAAAGAAGAT
GCTCGCGGCCGTAAAGCCAGCTTTGTAATCAACCTCAAAGAGCTCAAAACCCGCGAGTTACCTGATCTTGACGATGCCTT
CGCCCAGCAGTCCAGTGACAAAGCAACTCTTGAGGAACTTCGCAACGATCTAGAACAACGTCTTCAGGAAGACGCAAAGC
GTCGTGATCGCAGCAATCGTCACGACGCACTGCTGGAAGCATTGACTGAGCAGCTGGAAGTAGATCTACCAAACACCCTT
GTGCAGCAAGAGATACGCAACCTTGTTGAACAAACTGCCAGCCAATTCGCTCAACAGGGAATGGACGTGAAATCCATGTT
CACCCCAGAGCTTGTGCGTTCCTTGATGGAATCATCACGCCCAGAAGCCGAAGAGCGGCTAAGACGCAGTTTTGCCCTCA
CTGCACTAGCTGAAAGCGAAGATCTCAAAATAGAAGAGAGTGAAATCAGCGCCAAGGTCAAGGAAGTAAGCCGCGAGCTT
TCTGGCGAACGCGATATTGACCCGGCAAGGTTGCGTCAAGCTGTTAGCGACGACTTACTCAAAGACAAATTGCTGGACTG
GCTCGAAGACAACAGCACCATTACCGAAAAGGTACTTGAAAGCGAGGCAAAAACCAGTAAGCCCGCTGCAAAATCCAAAG
GCAGCAAAACTAAATCCACAAAAACCAAGACCAACAAAGCCAACACTGAAAAACCTGCCTCGGACAAAAGCAAGTCCTGA

Upstream 100 bases:

>100_bases
CCCTACAGCCTCAATCATGCAAGACGTTGAGACCACAATACGTGTTGAGAGATGAACTGGCGCTTTCTTAGGGTGGTCTT
CTGCTCGCAGTCGTCCGTCC

Downstream 100 bases:

>100_bases
ATGGCACCAATAGATTGATAGAAGTGCTCCCCTAAAGCCCAAGCGAGTGATCGATTCCTACTGCTACCACCCCATCCACA
ATCGCTGGCAAGGCATTCGT

Product: trigger factor

Products: peptidylproline (w =0) [C]

Alternate protein names: TF [H]

Number of amino acids: Translated: 479; Mature: 478

Protein sequence:

>479_residues
MSATALQVKTTQKPNSRLAVEVAVPAERCQANYEAAVTRLSRTTNLPGFRKGKVPRAVLLQQIGPVRIRATALESLVDAV
WREVLEQESIEPLCEPELSGGFDALLESFQPSEALTLTLETDVTPTPKLKATKGLQAEAEVVTFDPSKVDELIEQSRKQL
ATLVPVESRPAAIGDIAVVSFSGTYDDDGSAIEGGSSDSMDVDLEDGQMIPGFVEGIIGMNLGDEKTVDCHFPDDYSKED
ARGRKASFVINLKELKTRELPDLDDAFAQQSSDKATLEELRNDLEQRLQEDAKRRDRSNRHDALLEALTEQLEVDLPNTL
VQQEIRNLVEQTASQFAQQGMDVKSMFTPELVRSLMESSRPEAEERLRRSFALTALAESEDLKIEESEISAKVKEVSREL
SGERDIDPARLRQAVSDDLLKDKLLDWLEDNSTITEKVLESEAKTSKPAAKSKGSKTKSTKTKTNKANTEKPASDKSKS

Sequences:

>Translated_479_residues
MSATALQVKTTQKPNSRLAVEVAVPAERCQANYEAAVTRLSRTTNLPGFRKGKVPRAVLLQQIGPVRIRATALESLVDAV
WREVLEQESIEPLCEPELSGGFDALLESFQPSEALTLTLETDVTPTPKLKATKGLQAEAEVVTFDPSKVDELIEQSRKQL
ATLVPVESRPAAIGDIAVVSFSGTYDDDGSAIEGGSSDSMDVDLEDGQMIPGFVEGIIGMNLGDEKTVDCHFPDDYSKED
ARGRKASFVINLKELKTRELPDLDDAFAQQSSDKATLEELRNDLEQRLQEDAKRRDRSNRHDALLEALTEQLEVDLPNTL
VQQEIRNLVEQTASQFAQQGMDVKSMFTPELVRSLMESSRPEAEERLRRSFALTALAESEDLKIEESEISAKVKEVSREL
SGERDIDPARLRQAVSDDLLKDKLLDWLEDNSTITEKVLESEAKTSKPAAKSKGSKTKSTKTKTNKANTEKPASDKSKS
>Mature_478_residues
SATALQVKTTQKPNSRLAVEVAVPAERCQANYEAAVTRLSRTTNLPGFRKGKVPRAVLLQQIGPVRIRATALESLVDAVW
REVLEQESIEPLCEPELSGGFDALLESFQPSEALTLTLETDVTPTPKLKATKGLQAEAEVVTFDPSKVDELIEQSRKQLA
TLVPVESRPAAIGDIAVVSFSGTYDDDGSAIEGGSSDSMDVDLEDGQMIPGFVEGIIGMNLGDEKTVDCHFPDDYSKEDA
RGRKASFVINLKELKTRELPDLDDAFAQQSSDKATLEELRNDLEQRLQEDAKRRDRSNRHDALLEALTEQLEVDLPNTLV
QQEIRNLVEQTASQFAQQGMDVKSMFTPELVRSLMESSRPEAEERLRRSFALTALAESEDLKIEESEISAKVKEVSRELS
GERDIDPARLRQAVSDDLLKDKLLDWLEDNSTITEKVLESEAKTSKPAAKSKGSKTKSTKTKTNKANTEKPASDKSKS

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation [H]

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain [H]

Homologues:

Organism=Escherichia coli, GI1786640, Length=395, Percent_Identity=27.3417721518987, Blast_Score=150, Evalue=2e-37,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001179
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881 [H]

Pfam domain/function: PF00254 FKBP_C; PF05698 Trigger_C; PF05697 Trigger_N [H]

EC number: NA

Molecular weight: Translated: 52861; Mature: 52729

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSATALQVKTTQKPNSRLAVEVAVPAERCQANYEAAVTRLSRTTNLPGFRKGKVPRAVLL
CCCCEEEEEECCCCCCCEEEEEECCHHHHHCHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
QQIGPVRIRATALESLVDAVWREVLEQESIEPLCEPELSGGFDALLESFQPSEALTLTLE
HHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCEEEEEEE
TDVTPTPKLKATKGLQAEAEVVTFDPSKVDELIEQSRKQLATLVPVESRPAAIGDIAVVS
CCCCCCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEE
FSGTYDDDGSAIEGGSSDSMDVDLEDGQMIPGFVEGIIGMNLGDEKTVDCHFPDDYSKED
ECCCCCCCCCEECCCCCCCCEEECCCCCCCCHHHHHHHCCCCCCCCEEECCCCCCCCCHH
ARGRKASFVINLKELKTRELPDLDDAFAQQSSDKATLEELRNDLEQRLQEDAKRRDRSNR
CCCCCEEEEEEHHHHHCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCH
HDALLEALTEQLEVDLPNTLVQQEIRNLVEQTASQFAQQGMDVKSMFTPELVRSLMESSR
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHCCC
PEAEERLRRSFALTALAESEDLKIEESEISAKVKEVSRELSGERDIDPARLRQAVSDDLL
CCHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
KDKLLDWLEDNSTITEKVLESEAKTSKPAAKSKGSKTKSTKTKTNKANTEKPASDKSKS
HHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SATALQVKTTQKPNSRLAVEVAVPAERCQANYEAAVTRLSRTTNLPGFRKGKVPRAVLL
CCCEEEEEECCCCCCCEEEEEECCHHHHHCHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
QQIGPVRIRATALESLVDAVWREVLEQESIEPLCEPELSGGFDALLESFQPSEALTLTLE
HHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCEEEEEEE
TDVTPTPKLKATKGLQAEAEVVTFDPSKVDELIEQSRKQLATLVPVESRPAAIGDIAVVS
CCCCCCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEE
FSGTYDDDGSAIEGGSSDSMDVDLEDGQMIPGFVEGIIGMNLGDEKTVDCHFPDDYSKED
ECCCCCCCCCEECCCCCCCCEEECCCCCCCCHHHHHHHCCCCCCCCEEECCCCCCCCCHH
ARGRKASFVINLKELKTRELPDLDDAFAQQSSDKATLEELRNDLEQRLQEDAKRRDRSNR
CCCCCEEEEEEHHHHHCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCH
HDALLEALTEQLEVDLPNTLVQQEIRNLVEQTASQFAQQGMDVKSMFTPELVRSLMESSR
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHCCC
PEAEERLRRSFALTALAESEDLKIEESEISAKVKEVSRELSGERDIDPARLRQAVSDDLL
CCHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
KDKLLDWLEDNSTITEKVLESEAKTSKPAAKSKGSKTKSTKTKTNKANTEKPASDKSKS
HHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (w =180) [C]

Specific reaction: peptidylproline (w =180) = peptidylproline (w =0) [C]

General reaction: Isomerases; cis-trans-Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA