Definition Vibrio vulnificus CMCP6 chromosome chromosome I, complete sequence.
Accession NC_004459
Length 3,281,866

Click here to switch to the map view.

The map label for this gene is murE

Identifier: 326423735

GI number: 326423735

Start: 598062

End: 599543

Strand: Reverse

Name: murE

Synonym: NA

Alternate gene names: 326423735

Gene position: 599543-598062 (Counterclockwise)

Preceding gene: 326423736

Following gene: 27364043

Centisome position: 18.27

GC content: 50.74

Gene sequence:

>1482_bases
ATGCGAAATACCATGAATCTGACAAATTTATTAGCGCCGTGGCTAGATTGTCCTGAGCTTGCGGACATTACCGTCCAATC
GCTGGAGTTAGACAGTCGTCAGGTGAAACAAGGCGATACTTTTGTCGCTATCGTTGGTCACGTGGTGGATGGCCGCCAGT
ATATTGAGAAAGCAATAGAACAGGGCGCAAATGCCGTCATTGCTCAATCTTGTCAGCAATACCCTTCCGGCTTGGTGCGC
TATCAGCAAAACGTGGTGATTGTCTATCTTGAGAAGCTGGACGAAAAACTGTCGCAATTAGCGGGACGCCTGTACCAACA
TCCTGAGATGTCATTAATTGGTGTGACAGGAACCAATGGCAAAACCACCATCACTCAATTGATTGCGCAATGGCTAGAGT
TAGCCGGGCAAAAAGCCGCGGTGATGGGGACGACGGGCAACGGTTTTCTCAATGCCTTACAGCCTGCGGCCAATACCACG
GGCAATGCGGTTGAGATTCAAAAAACGTTGGCAGACTTACAGCAGCAAGGGGCAAAAGCAACTGCGTTGGAAGTCTCTTC
TCATGGCTTAGTGCAGGGGCGCGTTAAGGCGCTACAGTTTGCAGCTGGCGTGTTTACCAATCTGAGTCGTGATCATTTGG
ATTATCACGGCACGATGGAAGCCTATGCCAAAGCCAAAATGACGCTGTTTACTGAGCACCAGTGCCAGCATGCAATCATC
AATTTGGATGACGAAGTCGGTGCGCAGTGGTTTCAAGAGCTGCAACAAGGGGTCGGCGTTTCATTGTTTCCACAAGATGC
AAGTGTAAAAGCACTGTGGGCCAGCTCGGTAGCTTACGCAGAAAGCGGTATTACGATTGAGTTTGATGGCTGTTTTGGCC
AAGGGCGTCTGCACGCGCCATTGATTGGTGAATTTAATGCCACCAACTTACTGCTCGCGTTGGCAACACTGTTAGCCTTA
GGCGTTGATAAGCAAGCTTTATTGGATTCGGCCGCAAGCCTGCGTCCAGTACTTGGACGTATGGAACTGTTTCAAGTGAA
CAGCAAAGCAAAAGTGGTGGTCGATTATGCACATACCCCAGATGCGCTGGAGAAAGCGCTGCAAGCACTGCGTGTTCACT
GTACCGGTCATTTGTGGGCGATTTTCGGCTGTGGTGGTGATCGCGATAAAGGCAAGCGACCAATGATGGCTGAGATTGCA
GAGCGTCTGGCCGATCACGTCGTGCTTACCGACGACAACCCACGCAGTGAAGACCCCACCATGATTGTGCAAGATATGCT
GGCGGGCTTAACCCGTGCGGACAGTGCGGTGGTTGAGCACGATCGCTTCAGTGCGCTTCAATACGCGCTCGACAATGCAC
AAGCGGATGACATCATTTTGTTGGCAGGCAAAGGCCACGAAGATTATCAAGTACTCAAGCACCAGACGGTTCACTACTCG
GATCGTGAATCGGCGCAGCAACTGCTAGGAATTTCATCATGA

Upstream 100 bases:
NA

Downstream 100 bases:
NA

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase

Number of amino acids: Translated: 493; Mature: 493

Protein sequence:
NA

Sequences:

>Translated_493_residues
MRNTMNLTNLLAPWLDCPELADITVQSLELDSRQVKQGDTFVAIVGHVVDGRQYIEKAIEQGANAVIAQSCQQYPSGLVR
YQQNVVIVYLEKLDEKLSQLAGRLYQHPEMSLIGVTGTNGKTTITQLIAQWLELAGQKAAVMGTTGNGFLNALQPAANTT
GNAVEIQKTLADLQQQGAKATALEVSSHGLVQGRVKALQFAAGVFTNLSRDHLDYHGTMEAYAKAKMTLFTEHQCQHAII
NLDDEVGAQWFQELQQGVGVSLFPQDASVKALWASSVAYAESGITIEFDGCFGQGRLHAPLIGEFNATNLLLALATLLAL
GVDKQALLDSAASLRPVLGRMELFQVNSKAKVVVDYAHTPDALEKALQALRVHCTGHLWAIFGCGGDRDKGKRPMMAEIA
ERLADHVVLTDDNPRSEDPTMIVQDMLAGLTRADSAVVEHDRFSALQYALDNAQADDIILLAGKGHEDYQVLKHQTVHYS
DRESAQQLLGISS
>Mature_493_residues
MRNTMNLTNLLAPWLDCPELADITVQSLELDSRQVKQGDTFVAIVGHVVDGRQYIEKAIEQGANAVIAQSCQQYPSGLVR
YQQNVVIVYLEKLDEKLSQLAGRLYQHPEMSLIGVTGTNGKTTITQLIAQWLELAGQKAAVMGTTGNGFLNALQPAANTT
GNAVEIQKTLADLQQQGAKATALEVSSHGLVQGRVKALQFAAGVFTNLSRDHLDYHGTMEAYAKAKMTLFTEHQCQHAII
NLDDEVGAQWFQELQQGVGVSLFPQDASVKALWASSVAYAESGITIEFDGCFGQGRLHAPLIGEFNATNLLLALATLLAL
GVDKQALLDSAASLRPVLGRMELFQVNSKAKVVVDYAHTPDALEKALQALRVHCTGHLWAIFGCGGDRDKGKRPMMAEIA
ERLADHVVLTDDNPRSEDPTMIVQDMLAGLTRADSAVVEHDRFSALQYALDNAQADDIILLAGKGHEDYQVLKHQTVHYS
DRESAQQLLGISS

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily

Homologues:

Organism=Escherichia coli, GI1786273, Length=491, Percent_Identity=54.3788187372709, Blast_Score=480, Evalue=1e-136,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURE_VIBVU (Q8DEK5)

Other databases:

- EMBL:   AE016795
- RefSeq:   NP_759572.1
- ProteinModelPortal:   Q8DEK5
- SMR:   Q8DEK5
- GeneID:   1177534
- GenomeReviews:   AE016795_GR
- KEGG:   vvu:VV1_0583
- HOGENOM:   HBG602753
- OMA:   VLGCGGD
- ProtClustDB:   PRK00139
- BioCyc:   VVUL216895:VV1_0583-MONOMER
- BRENDA:   6.3.2.13
- GO:   GO:0005737
- HAMAP:   MF_00208
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- TIGRFAMs:   TIGR01085

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.13

Molecular weight: Translated: 53594; Mature: 53594

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: NA

Important sites: BINDING 30-30 BINDING 32-32 BINDING 158-158 BINDING 186-186 BINDING 192-192 BINDING 194-194 BINDING 388-388 BINDING 463-463 BINDING 467-467

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure: 

>Translated Secondary Structure
MRNTMNLTNLLAPWLDCPELADITVQSLELDSRQVKQGDTFVAIVGHVVDGRQYIEKAIE
CCCCCCHHHHHHHHCCCCCHHHCCHHHHCCCHHHHHCCCEEEEEHHHHHCCHHHHHHHHH
QGANAVIAQSCQQYPSGLVRYQQNVVIVYLEKLDEKLSQLAGRLYQHPEMSLIGVTGTNG
CCCCHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHCCCCCEEEEEECCCC
KTTITQLIAQWLELAGQKAAVMGTTGNGFLNALQPAANTTGNAVEIQKTLADLQQQGAKA
CHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCE
TALEVSSHGLVQGRVKALQFAAGVFTNLSRDHLDYHGTMEAYAKAKMTLFTEHQCQHAII
EEEEECCCCCHHHHHHHHHHHHHHHHCCCHHHCCCCCHHHHHHHHHHHEEEHHCCCHHEE
NLDDEVGAQWFQELQQGVGVSLFPQDASVKALWASSVAYAESGITIEFDGCFGQGRLHAP
CCCHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCEECCC
LIGEFNATNLLLALATLLALGVDKQALLDSAASLRPVLGRMELFQVNSKAKVVVDYAHTP
EECCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHEEECCCCEEEEEECCCC
DALEKALQALRVHCTGHLWAIFGCGGDRDKGKRPMMAEIAERLADHVVLTDDNPRSEDPT
HHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHCCEEEECCCCCCCCCH
MIVQDMLAGLTRADSAVVEHDRFSALQYALDNAQADDIILLAGKGHEDYQVLKHQTVHYS
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHCCCC
DRESAQQLLGISS
CHHHHHHHHCCCC
>Mature Secondary Structure
MRNTMNLTNLLAPWLDCPELADITVQSLELDSRQVKQGDTFVAIVGHVVDGRQYIEKAIE
CCCCCCHHHHHHHHCCCCCHHHCCHHHHCCCHHHHHCCCEEEEEHHHHHCCHHHHHHHHH
QGANAVIAQSCQQYPSGLVRYQQNVVIVYLEKLDEKLSQLAGRLYQHPEMSLIGVTGTNG
CCCCHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHCCCCCEEEEEECCCC
KTTITQLIAQWLELAGQKAAVMGTTGNGFLNALQPAANTTGNAVEIQKTLADLQQQGAKA
CHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCE
TALEVSSHGLVQGRVKALQFAAGVFTNLSRDHLDYHGTMEAYAKAKMTLFTEHQCQHAII
EEEEECCCCCHHHHHHHHHHHHHHHHCCCHHHCCCCCHHHHHHHHHHHEEEHHCCCHHEE
NLDDEVGAQWFQELQQGVGVSLFPQDASVKALWASSVAYAESGITIEFDGCFGQGRLHAP
CCCHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCEECCC
LIGEFNATNLLLALATLLALGVDKQALLDSAASLRPVLGRMELFQVNSKAKVVVDYAHTP
EECCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHEEECCCCEEEEEECCCC
DALEKALQALRVHCTGHLWAIFGCGGDRDKGKRPMMAEIAERLADHVVLTDDNPRSEDPT
HHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHCCEEEECCCCCCCCCH
MIVQDMLAGLTRADSAVVEHDRFSALQYALDNAQADDIILLAGKGHEDYQVLKHQTVHYS
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHCCCC
DRESAQQLLGISS
CHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA