| Definition | Vibrio vulnificus CMCP6 chromosome chromosome I, complete sequence. |
|---|---|
| Accession | NC_004459 |
| Length | 3,281,866 |
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The map label for this gene is murF [H]
Identifier: 27364043
GI number: 27364043
Start: 596704
End: 598065
Strand: Reverse
Name: murF [H]
Synonym: VV1_0582
Alternate gene names: 27364043
Gene position: 598065-596704 (Counterclockwise)
Preceding gene: 326423735
Following gene: 27364042
Centisome position: 18.22
GC content: 49.27
Gene sequence:
>1362_bases ATGATTGAGACCTCTCTGAGTCATTTGGCGCAATTGACGCAAGGACAACTGATTGGCGATGACGCCATCTTTTGTACCGT TTCAACGGATACGCGTCATATCCCTCAGCAAGCGTTATTTATTGCGCTCGTCGGTGAACGTTTTGATGCGCATGATTTTT GCCAGCAAGCGGTGGACGCAGGTGCTATTGCATTGTTGGTGGAGCGAGCGCTGGATCTTCCCGTTGCACAAGTAGTGGTC AAAGATACGAAAATTGCACTCGGTCAGCTCGGAGCGAATGTGCATCAGCGCTGCCAAACTCCAACCGTGGCGATCACGGG CAGTTGCGGCAAAACCACGGTGAAAGAGATGGTGGCCAGTATTCTGCAGCAAAAAGGCAAAGTGCTGTTCACGGCAGGTA ATTTTAACAATGACATTGGCGCGCCTCTGACGTTGCTACGCTCTGAGCCAGAAGATGATTTTGCGGTGATTGAACTGGGC GCCAATCATATTGGTGAAATTGCCTATACCACCCAGCTTGTTCGGCCCGATGTGGCGTTGGTGAACAACGTGGCTGCGGC GCATCTTGAAGGGTTTGGCTCGATTGATGGCGTGAAACAAGCGAAAGGGGAGATCTACCAAGGTTTGTCTGCTGGTGGCG TGGCCATCGTCAATCTAGACAGTCATGGAGGTGAGTACTGGACACAGGTACTGGCCGATAAACAGGTCACGACGTTTTCG GCAAGCTCCGAGCAGGCCGATTTCTACGCAACAGACATCGTACTGGATACGCAAGGGAAAGCGTCATTCGTATTGCATAC GCCTGAAGAACGCATTCCACTTTCTCTTGGTATCATTGGCCAACATAACGTCGCCAATGCGATTGCCGCTAGCATCATTG CTTTGCAGTTTGGTGCCAGCTTAGCGCAAATCCAATCGGGATTGCTCAATTTGATGCCTGTCAAAGGGCGTGTTGATGTT CAACAGTTATCCGACAACATCAAACTGATTGATGACAGTTATAATGCCAGTGTGCCAGCTATGAAGGCGGCGGTGGACCT ATTAGGTAGTTTTACCGGACAGCGTTGGTTAGTTTTAGGTTATATGGCGGAATTGGGCAATGAAAGTCTTGCACTTCATC GTCAAGTCGGTGAACATGCTGCCCCATTAAATTTTGAACACGTCCTTACCTATGGCGAGGATACAAGGATCATTAGCCAG TTGTGTGGTGGAAAACATTTTTCAGACCATGGTGAACTGATCGAATACATCAAGCAGAATTTGGCCAACGAAAATGAAGC CTGTCACACCGTGTTGGTCAAAGGCGCAAACTCAGCTGGCATGAGTAAAGTGGCTGCTGCTTTGAAGGAGAACTATCGAT GA
Upstream 100 bases:
>100_bases TGTTGGCAGGCAAAGGCCACGAAGATTATCAAGTACTCAAGCACCAGACGGTTCACTACTCGGATCGTGAATCGGCGCAG CAACTGCTAGGAATTTCATC
Downstream 100 bases:
>100_bases TTATTTGGCTTGCCGAGCTGCTACAGCCATACTTTTCCTTTTTCCGACTGTTTGAATACCTGTCGTTTCGCGCGATCTTA AGTGTTCTAACCGCGTTGGG
Product: UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate--D-alanyl-D- alanyl ligase
Products: NA
Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]
Number of amino acids: Translated: 453; Mature: 453
Protein sequence:
>453_residues MIETSLSHLAQLTQGQLIGDDAIFCTVSTDTRHIPQQALFIALVGERFDAHDFCQQAVDAGAIALLVERALDLPVAQVVV KDTKIALGQLGANVHQRCQTPTVAITGSCGKTTVKEMVASILQQKGKVLFTAGNFNNDIGAPLTLLRSEPEDDFAVIELG ANHIGEIAYTTQLVRPDVALVNNVAAAHLEGFGSIDGVKQAKGEIYQGLSAGGVAIVNLDSHGGEYWTQVLADKQVTTFS ASSEQADFYATDIVLDTQGKASFVLHTPEERIPLSLGIIGQHNVANAIAASIIALQFGASLAQIQSGLLNLMPVKGRVDV QQLSDNIKLIDDSYNASVPAMKAAVDLLGSFTGQRWLVLGYMAELGNESLALHRQVGEHAAPLNFEHVLTYGEDTRIISQ LCGGKHFSDHGELIEYIKQNLANENEACHTVLVKGANSAGMSKVAAALKENYR
Sequences:
>Translated_453_residues MIETSLSHLAQLTQGQLIGDDAIFCTVSTDTRHIPQQALFIALVGERFDAHDFCQQAVDAGAIALLVERALDLPVAQVVV KDTKIALGQLGANVHQRCQTPTVAITGSCGKTTVKEMVASILQQKGKVLFTAGNFNNDIGAPLTLLRSEPEDDFAVIELG ANHIGEIAYTTQLVRPDVALVNNVAAAHLEGFGSIDGVKQAKGEIYQGLSAGGVAIVNLDSHGGEYWTQVLADKQVTTFS ASSEQADFYATDIVLDTQGKASFVLHTPEERIPLSLGIIGQHNVANAIAASIIALQFGASLAQIQSGLLNLMPVKGRVDV QQLSDNIKLIDDSYNASVPAMKAAVDLLGSFTGQRWLVLGYMAELGNESLALHRQVGEHAAPLNFEHVLTYGEDTRIISQ LCGGKHFSDHGELIEYIKQNLANENEACHTVLVKGANSAGMSKVAAALKENYR >Mature_453_residues MIETSLSHLAQLTQGQLIGDDAIFCTVSTDTRHIPQQALFIALVGERFDAHDFCQQAVDAGAIALLVERALDLPVAQVVV KDTKIALGQLGANVHQRCQTPTVAITGSCGKTTVKEMVASILQQKGKVLFTAGNFNNDIGAPLTLLRSEPEDDFAVIELG ANHIGEIAYTTQLVRPDVALVNNVAAAHLEGFGSIDGVKQAKGEIYQGLSAGGVAIVNLDSHGGEYWTQVLADKQVTTFS ASSEQADFYATDIVLDTQGKASFVLHTPEERIPLSLGIIGQHNVANAIAASIIALQFGASLAQIQSGLLNLMPVKGRVDV QQLSDNIKLIDDSYNASVPAMKAAVDLLGSFTGQRWLVLGYMAELGNESLALHRQVGEHAAPLNFEHVLTYGEDTRIISQ LCGGKHFSDHGELIEYIKQNLANENEACHTVLVKGANSAGMSKVAAALKENYR
Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]
COG id: COG0770
COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase
Gene ontology:
Cell location: Cytoplasm [H]
Metaboloic importance: Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the MurCDEF family [H]
Homologues:
Organism=Escherichia coli, GI1786274, Length=452, Percent_Identity=50.2212389380531, Blast_Score=388, Evalue=1e-109,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR004101 - InterPro: IPR013221 - InterPro: IPR000713 - InterPro: IPR005863 [H]
Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]
EC number: =6.3.2.10 [H]
Molecular weight: Translated: 48344; Mature: 48344
Theoretical pI: Translated: 5.01; Mature: 5.01
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.3 %Cys (Translated Protein) 1.3 %Met (Translated Protein) 2.6 %Cys+Met (Translated Protein) 1.3 %Cys (Mature Protein) 1.3 %Met (Mature Protein) 2.6 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MIETSLSHLAQLTQGQLIGDDAIFCTVSTDTRHIPQQALFIALVGERFDAHDFCQQAVDA CCCCHHHHHHHHHCCCEECCCEEEEEECCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHCC GAIALLVERALDLPVAQVVVKDTKIALGQLGANVHQRCQTPTVAITGSCGKTTVKEMVAS HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHCCCCCEEEECCCCHHHHHHHHHH ILQQKGKVLFTAGNFNNDIGAPLTLLRSEPEDDFAVIELGANHIGEIAYTTQLVRPDVAL HHHHCCCEEEEECCCCCCCCCCHHEECCCCCCCEEEEEECCCHHHHHHHHHHHHCCCHHH VNNVAAAHLEGFGSIDGVKQAKGEIYQGLSAGGVAIVNLDSHGGEYWTQVLADKQVTTFS HHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHCCCEEEEE ASSEQADFYATDIVLDTQGKASFVLHTPEERIPLSLGIIGQHNVANAIAASIIALQFGAS CCCCCCCEEEEEEEEECCCCEEEEEECCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCH LAQIQSGLLNLMPVKGRVDVQQLSDNIKLIDDSYNASVPAMKAAVDLLGSFTGQRWLVLG HHHHHHCHHEEECCCCCCCHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCHHHHHH YMAELGNESLALHRQVGEHAAPLNFEHVLTYGEDTRIISQLCGGKHFSDHGELIEYIKQN HHHHHCCCHHHHHHHHCCCCCCCCHHHEEECCCHHHHHHHHHCCCCCCCHHHHHHHHHHH LANENEACHTVLVKGANSAGMSKVAAALKENYR HCCCCCCEEEEEEECCCCCCHHHHHHHHHHCCC >Mature Secondary Structure MIETSLSHLAQLTQGQLIGDDAIFCTVSTDTRHIPQQALFIALVGERFDAHDFCQQAVDA CCCCHHHHHHHHHCCCEECCCEEEEEECCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHCC GAIALLVERALDLPVAQVVVKDTKIALGQLGANVHQRCQTPTVAITGSCGKTTVKEMVAS HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHCCCCCEEEECCCCHHHHHHHHHH ILQQKGKVLFTAGNFNNDIGAPLTLLRSEPEDDFAVIELGANHIGEIAYTTQLVRPDVAL HHHHCCCEEEEECCCCCCCCCCHHEECCCCCCCEEEEEECCCHHHHHHHHHHHHCCCHHH VNNVAAAHLEGFGSIDGVKQAKGEIYQGLSAGGVAIVNLDSHGGEYWTQVLADKQVTTFS HHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHCCCEEEEE ASSEQADFYATDIVLDTQGKASFVLHTPEERIPLSLGIIGQHNVANAIAASIIALQFGAS CCCCCCCEEEEEEEEECCCCEEEEEECCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCH LAQIQSGLLNLMPVKGRVDVQQLSDNIKLIDDSYNASVPAMKAAVDLLGSFTGQRWLVLG HHHHHHCHHEEECCCCCCCHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCHHHHHH YMAELGNESLALHRQVGEHAAPLNFEHVLTYGEDTRIISQLCGGKHFSDHGELIEYIKQN HHHHHCCCHHHHHHHHCCCCCCCCHHHEEECCCHHHHHHHHHCCCCCCCHHHHHHHHHHH LANENEACHTVLVKGANSAGMSKVAAALKENYR HCCCCCCEEEEEEECCCCCCHHHHHHHHHHCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 2668880; 8973200; 1630901; 9278503; 9166795; 2186811; 11090285 [H]