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The map label for this gene is pitB [C]

Identifier: 32267370

GI number: 32267370

Start: 1791499

End: 1793088

Strand: Direct

Name: pitB [C]

Synonym: HH1871

Alternate gene names: 32267370

Gene position: 1791499-1793088 (Clockwise)

Preceding gene: 32267369

Following gene: 32267371

Centisome position: 99.57

GC content: 37.61

Gene sequence:

>1590_bases
ATGGAGTTTAAAAATGTCAAGCAAATTGAAAAGGCTCAGAACTTTAACCAAAAAGATGGTATAAAGCTTTTTATTGTACT
CATCTTTTTTCTAGTTATTTCTCTGATAGCAATTTTTGTTCATACAGGATATAACACACTTTTACTTGTTTTTGCAACTA
TCATCGGTGGCTATATGGCGATGAGCATTGGAGCAAATGATGTGGCAAACAATGTTGGACCAGCAGTTGGCTCCCACGCA
ATTACACTTGTAGGGGCGATTATAATTGCTGCAATTTGTGAAGCTATGGGAGCAGTTATCGCTGGTGGGGAAGTCGTAGA
GACGATTAAATCAGGTATTATAGATTCTCAAAATATCACTGACCCAAGTGTGTTTGTGGCTCTTATGCTAGCAGCGCTTG
CTTCAGGTGCAGTATGGTTACACCTTGCTACTGCTATCGGAGCTCCTGTTTCAACTACACATTCTCTTGTAGGCGGAATC
TTAGGTGCTGGAGTTGTTGCAGTAGGCTTTGAAATTGTAAATTGGGGAGAGTGCTTACGCATTGTATCAAGTTGGGTTAT
CTCACCTGTGCTTGGTGGTTTGATTGCAGTAATTTTTCTTATCATCATTAAAAAAAGCATTACCTATAAAGAAGATAAAC
GTGAGGCAGCCAAAAAAGTAGTGCCTATTCTCGTTTTTGTAATGACTTGGGCTTTTAGTCTTTACCTTATACTCAAAGGT
TTAAAAAAGATATTACCATCGCTTGATTTGGTTTTATCTGTAGGCATTAGCTTTATTATAGCGATTGGGGTTTATTTTAT
CGTGCGCCCTATCATAGCCAAGAAAGCAGATTCTCTCCTAAACACCAAAGAAGACATTAATTCACTCTTTACTATTCCTC
TTGTTTTTTCAGCAGCACTTTTAAGCTTCGCTCACGGAGCAAATGATGTTGCAAACGCTATTGGACCACTCGCAGCAATT
AATGAAACGTTAAAAAATCTTAGTGAATCTCTCCCTACAAGTAAAGCAGGTGTGCCATTATGGATTATGCTGGTAGGTGG
GCTTGGTATTTCTCTAGGACTTGCACTCTATGGACCAAGACTTATCCGCACGGTGGGAAGCGAAATTACTGAATTAGATA
AAATAAGAGCATTTTGTATCGCTATGAGCGCAGCCTTAACTGTTCTCTTGGCTTCCCAACTTGGATTACCTGTAAGCTCA
ACACATATTGCCATTGGAGCAATATTTGGTGTGGGATTCTTACGTGAATATCTTAAAAAACGCTATTATGAAATGCAGCA
AATCATCATAAAAGCTCATAAGGGGAAAGATGCTCAAGAAGTAGAGGCATTTTTAGAGCGTTTTAATAAAGCAAGCATTA
AGAAAAAAGGTTTGATGTTAGAATCTATTAAGAATAATAGACAACTCAAAGATATACCATATTTTGATAGAAAAGAGCGA
AAATCACTTAAGAAAGCTTATAAACAAGAGTTAGTAAAACGTAATGCGATTAATAAAATAATTGCTTCTTGGCTTATCAC
CGTGCCTGTATCTGCTGTTCTTGGCGGAGTTACTTATTATGTGATTACTAGTCTTGGCTTTAGTATTTAG

Upstream 100 bases:

>100_bases
TTCCTTGGCTTGGAATAATGTTGGTGGGTTTATTGCTTATAACTTATATTCCAGATATTTCGCTTTGGTTACCTAATAAA
CTCAATTAAAAGGAGGATTT

Downstream 100 bases:

>100_bases
TTTTTTAAAAGATTTGATTGATTACAATTAAATCTTTACTTTTAGACAAGGAGATTTTTTGGACCCGTATTCGTCCATTT
TAATGCTTATTTTGGCATTA

Product: phosphate permease

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 529; Mature: 529

Protein sequence:

>529_residues
MEFKNVKQIEKAQNFNQKDGIKLFIVLIFFLVISLIAIFVHTGYNTLLLVFATIIGGYMAMSIGANDVANNVGPAVGSHA
ITLVGAIIIAAICEAMGAVIAGGEVVETIKSGIIDSQNITDPSVFVALMLAALASGAVWLHLATAIGAPVSTTHSLVGGI
LGAGVVAVGFEIVNWGECLRIVSSWVISPVLGGLIAVIFLIIIKKSITYKEDKREAAKKVVPILVFVMTWAFSLYLILKG
LKKILPSLDLVLSVGISFIIAIGVYFIVRPIIAKKADSLLNTKEDINSLFTIPLVFSAALLSFAHGANDVANAIGPLAAI
NETLKNLSESLPTSKAGVPLWIMLVGGLGISLGLALYGPRLIRTVGSEITELDKIRAFCIAMSAALTVLLASQLGLPVSS
THIAIGAIFGVGFLREYLKKRYYEMQQIIIKAHKGKDAQEVEAFLERFNKASIKKKGLMLESIKNNRQLKDIPYFDRKER
KSLKKAYKQELVKRNAINKIIASWLITVPVSAVLGGVTYYVITSLGFSI

Sequences:

>Translated_529_residues
MEFKNVKQIEKAQNFNQKDGIKLFIVLIFFLVISLIAIFVHTGYNTLLLVFATIIGGYMAMSIGANDVANNVGPAVGSHA
ITLVGAIIIAAICEAMGAVIAGGEVVETIKSGIIDSQNITDPSVFVALMLAALASGAVWLHLATAIGAPVSTTHSLVGGI
LGAGVVAVGFEIVNWGECLRIVSSWVISPVLGGLIAVIFLIIIKKSITYKEDKREAAKKVVPILVFVMTWAFSLYLILKG
LKKILPSLDLVLSVGISFIIAIGVYFIVRPIIAKKADSLLNTKEDINSLFTIPLVFSAALLSFAHGANDVANAIGPLAAI
NETLKNLSESLPTSKAGVPLWIMLVGGLGISLGLALYGPRLIRTVGSEITELDKIRAFCIAMSAALTVLLASQLGLPVSS
THIAIGAIFGVGFLREYLKKRYYEMQQIIIKAHKGKDAQEVEAFLERFNKASIKKKGLMLESIKNNRQLKDIPYFDRKER
KSLKKAYKQELVKRNAINKIIASWLITVPVSAVLGGVTYYVITSLGFSI
>Mature_529_residues
MEFKNVKQIEKAQNFNQKDGIKLFIVLIFFLVISLIAIFVHTGYNTLLLVFATIIGGYMAMSIGANDVANNVGPAVGSHA
ITLVGAIIIAAICEAMGAVIAGGEVVETIKSGIIDSQNITDPSVFVALMLAALASGAVWLHLATAIGAPVSTTHSLVGGI
LGAGVVAVGFEIVNWGECLRIVSSWVISPVLGGLIAVIFLIIIKKSITYKEDKREAAKKVVPILVFVMTWAFSLYLILKG
LKKILPSLDLVLSVGISFIIAIGVYFIVRPIIAKKADSLLNTKEDINSLFTIPLVFSAALLSFAHGANDVANAIGPLAAI
NETLKNLSESLPTSKAGVPLWIMLVGGLGISLGLALYGPRLIRTVGSEITELDKIRAFCIAMSAALTVLLASQLGLPVSS
THIAIGAIFGVGFLREYLKKRYYEMQQIIIKAHKGKDAQEVEAFLERFNKASIKKKGLMLESIKNNRQLKDIPYFDRKER
KSLKKAYKQELVKRNAINKIIASWLITVPVSAVLGGVTYYVITSLGFSI

Specific function: Potential transporter for phosphate [H]

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family [H]

Homologues:

Organism=Homo sapiens, GI31543630, Length=292, Percent_Identity=32.8767123287671, Blast_Score=129, Evalue=6e-30,
Organism=Homo sapiens, GI5803173, Length=235, Percent_Identity=35.7446808510638, Blast_Score=126, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI25146401, Length=444, Percent_Identity=33.1081081081081, Blast_Score=209, Evalue=4e-54,
Organism=Caenorhabditis elegans, GI17557328, Length=475, Percent_Identity=27.1578947368421, Blast_Score=144, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI17539280, Length=207, Percent_Identity=34.7826086956522, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI32566716, Length=223, Percent_Identity=33.6322869955157, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI71981576, Length=133, Percent_Identity=42.8571428571429, Blast_Score=108, Evalue=8e-24,
Organism=Caenorhabditis elegans, GI17536725, Length=233, Percent_Identity=25.3218884120172, Blast_Score=84, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6319773, Length=227, Percent_Identity=38.7665198237885, Blast_Score=106, Evalue=1e-23,
Organism=Drosophila melanogaster, GI21356511, Length=234, Percent_Identity=34.6153846153846, Blast_Score=124, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001204 [H]

Pfam domain/function: PF01384 PHO4 [H]

EC number: NA

Molecular weight: Translated: 56880; Mature: 56880

Theoretical pI: Translated: 10.10; Mature: 10.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9252185 [H]