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The map label for this gene is hisS

Identifier: 32267308

GI number: 32267308

Start: 1731325

End: 1732653

Strand: Direct

Name: hisS

Synonym: HH1809

Alternate gene names: 32267308

Gene position: 1731325-1732653 (Clockwise)

Preceding gene: 32267302

Following gene: 32267309

Centisome position: 96.23

GC content: 35.74

Gene sequence:

>1329_bases
ATGGCGTTGGTTACCCCTCGCACATTAAGCGGTTTTAAAGATAGGTTGCCAAAAGAGGCGCTTGCAAAAGCACATTTACT
TAATAAAGTGAGCAATGTATTTATGAATTTTGGTTTCGTGCCTATTGAGACACCGCATTTAGAGTATGCTGATGTGCTTA
TTAAACAGGGTAGCGATGAAATACAAAAGGAACTCTATCGTTTTAAAGATCACGGTGGCCGCGATGTAGCATTGAGATTT
GATTTAACCGTGCCTTTAGCTCGTTTTGTATCACAATACAAAAATGAAGTAGGATTGCCTTTTAAACGATATGCGATTGG
AAATGTATTTCGTGGAGAGCGAGCACAAAGAGGAAGGTATAGAGAATTCACACAATGTGATTTTGACTTTATTGGGAGTG
ATAGTATCTCGTGTGATGCTGAAATTTTACAAGTCATTTATGCCTCATTGATGAAACTTGGCATCGAGGAATTTACTATT
TGGCTTAACCATCGTAGCATTCTAAATGGTATTTGTGAATATTGTGGTATTACACAAGAAAAAGATATAAATGTGGCTTT
GCGTATTATTGATAAGCTTGATAAAATTGGTAAAGAGCGTGTGAGCGAGGAGTTACAAAAGGAATTGGGTTTAAATAATA
ATCAAGTAGAGAATCTCTTAGAATACACTTCAATTAAGCAACAAGGTGAGAGTGAAAGCTTTTTTAGACAAATTTCATTT
ATGGAAGAATGGAATGATTCTATTAAAAAAGGCATTGAGGATTTAAAAATGCTTTATGAAGTGCTTTCATCTTTGCAAAT
GGATAGAGATACTTATCGTGTTAATTTTTCAATTGCTCGTGGATTAGGGTATTATACAGGCATTGTATATGAAACAACTC
TGAATGCCTTAAAAAGCATCGGGAGCGTATGTTCTGGCGGAAGATATGATAATCTTACGCGCACTTTTTCTAAAGAAAAA
ATGAGTGGTGTTGGCGCGAGTATTGGTATTGATAGGCTTTTAGCAGCACTTGAGGAGCTTGAGCTCTTACAAAAAAGAGC
GACATCTGCTCGCGCACTTATTGTGTGTATGGATAGACTATATTTTGGATATGCTCATTTACTTGCAGAATCTTTTCGTT
GTAGTGAGATTCCTATTGAAGTGTATCCAGAAGCTTCCAAACTTAAAAAACAATTTACTTATGCTCATTTGAAAGGATAT
GAGTTTGTAGTTGTCATTGGAGAGAATGAGTTTAATACAAAAACTCTCACACTTAAAAATATGACTTCAGGTGTGCAAAT
AGATAGCATAAGCTTTCTTAAAGCTCTTGCACTTATTCAGGAGGAGTAA

Upstream 100 bases:

>100_bases
GAAAAATTTGCCAAATTACCTCATAATGTAGCCAAAATAAAACATTTTTAAGGTAGAATCCACATACTTTAGTTTGTGAA
ATTTTAAAGGAGGTCTGCAG

Downstream 100 bases:

>100_bases
AATGAATTTAGAGCAAAATAAATTAAAAACACAAAGGAGCAGTATGAGAGGCATTATAGAATCTAGTCAATGGGATTTAA
CTGCACTTTTTAAGACAAAA

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS

Number of amino acids: Translated: 442; Mature: 441

Protein sequence:

>442_residues
MALVTPRTLSGFKDRLPKEALAKAHLLNKVSNVFMNFGFVPIETPHLEYADVLIKQGSDEIQKELYRFKDHGGRDVALRF
DLTVPLARFVSQYKNEVGLPFKRYAIGNVFRGERAQRGRYREFTQCDFDFIGSDSISCDAEILQVIYASLMKLGIEEFTI
WLNHRSILNGICEYCGITQEKDINVALRIIDKLDKIGKERVSEELQKELGLNNNQVENLLEYTSIKQQGESESFFRQISF
MEEWNDSIKKGIEDLKMLYEVLSSLQMDRDTYRVNFSIARGLGYYTGIVYETTLNALKSIGSVCSGGRYDNLTRTFSKEK
MSGVGASIGIDRLLAALEELELLQKRATSARALIVCMDRLYFGYAHLLAESFRCSEIPIEVYPEASKLKKQFTYAHLKGY
EFVVVIGENEFNTKTLTLKNMTSGVQIDSISFLKALALIQEE

Sequences:

>Translated_442_residues
MALVTPRTLSGFKDRLPKEALAKAHLLNKVSNVFMNFGFVPIETPHLEYADVLIKQGSDEIQKELYRFKDHGGRDVALRF
DLTVPLARFVSQYKNEVGLPFKRYAIGNVFRGERAQRGRYREFTQCDFDFIGSDSISCDAEILQVIYASLMKLGIEEFTI
WLNHRSILNGICEYCGITQEKDINVALRIIDKLDKIGKERVSEELQKELGLNNNQVENLLEYTSIKQQGESESFFRQISF
MEEWNDSIKKGIEDLKMLYEVLSSLQMDRDTYRVNFSIARGLGYYTGIVYETTLNALKSIGSVCSGGRYDNLTRTFSKEK
MSGVGASIGIDRLLAALEELELLQKRATSARALIVCMDRLYFGYAHLLAESFRCSEIPIEVYPEASKLKKQFTYAHLKGY
EFVVVIGENEFNTKTLTLKNMTSGVQIDSISFLKALALIQEE
>Mature_441_residues
ALVTPRTLSGFKDRLPKEALAKAHLLNKVSNVFMNFGFVPIETPHLEYADVLIKQGSDEIQKELYRFKDHGGRDVALRFD
LTVPLARFVSQYKNEVGLPFKRYAIGNVFRGERAQRGRYREFTQCDFDFIGSDSISCDAEILQVIYASLMKLGIEEFTIW
LNHRSILNGICEYCGITQEKDINVALRIIDKLDKIGKERVSEELQKELGLNNNQVENLLEYTSIKQQGESESFFRQISFM
EEWNDSIKKGIEDLKMLYEVLSSLQMDRDTYRVNFSIARGLGYYTGIVYETTLNALKSIGSVCSGGRYDNLTRTFSKEKM
SGVGASIGIDRLLAALEELELLQKRATSARALIVCMDRLYFGYAHLLAESFRCSEIPIEVYPEASKLKKQFTYAHLKGYE
FVVVIGENEFNTKTLTLKNMTSGVQIDSISFLKALALIQEE

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI6996014, Length=442, Percent_Identity=33.0316742081448, Blast_Score=201, Evalue=1e-51,
Organism=Homo sapiens, GI15029520, Length=437, Percent_Identity=31.5789473684211, Blast_Score=184, Evalue=2e-46,
Organism=Homo sapiens, GI65287717, Length=414, Percent_Identity=24.8792270531401, Blast_Score=93, Evalue=4e-19,
Organism=Escherichia coli, GI1788861, Length=429, Percent_Identity=27.7389277389277, Blast_Score=129, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI71993693, Length=446, Percent_Identity=34.0807174887892, Blast_Score=203, Evalue=1e-52,
Organism=Caenorhabditis elegans, GI71993686, Length=446, Percent_Identity=34.0807174887892, Blast_Score=203, Evalue=1e-52,
Organism=Saccharomyces cerevisiae, GI6325290, Length=466, Percent_Identity=30.68669527897, Blast_Score=185, Evalue=1e-47,
Organism=Saccharomyces cerevisiae, GI6320489, Length=342, Percent_Identity=24.5614035087719, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24643061, Length=462, Percent_Identity=31.6017316017316, Blast_Score=186, Evalue=2e-47,
Organism=Drosophila melanogaster, GI24643059, Length=481, Percent_Identity=29.9376299376299, Blast_Score=174, Evalue=7e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYH_HELHP (Q7VF68)

Other databases:

- EMBL:   AE017125
- RefSeq:   NP_861340.1
- ProteinModelPortal:   Q7VF68
- SMR:   Q7VF68
- GeneID:   1492356
- GenomeReviews:   AE017125_GR
- KEGG:   hhe:HH1809
- NMPDR:   fig|235279.1.peg.1809
- HOGENOM:   HBG616575
- OMA:   ALDIARC
- ProtClustDB:   PRK00037
- BioCyc:   HHEP235279:HH_1809-MONOMER
- BRENDA:   6.1.1.21
- GO:   GO:0005737
- HAMAP:   MF_00127
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516
- Gene3D:   G3DSA:3.40.50.800
- PANTHER:   PTHR11476
- PIRSF:   PIRSF001549
- TIGRFAMs:   TIGR00442

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd

EC number: =6.1.1.21

Molecular weight: Translated: 50383; Mature: 50251

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS50862 AA_TRNA_LIGASE_II; PS00012 PHOSPHOPANTETHEINE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALVTPRTLSGFKDRLPKEALAKAHLLNKVSNVFMNFGFVPIETPHLEYADVLIKQGSDE
CCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCEECCCCCCHHHHHHHHCCCHH
IQKELYRFKDHGGRDVALRFDLTVPLARFVSQYKNEVGLPFKRYAIGNVFRGERAQRGRY
HHHHHHHHHCCCCCEEEEEEECHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCHHHCCCC
REFTQCDFDFIGSDSISCDAEILQVIYASLMKLGIEEFTIWLNHRSILNGICEYCGITQE
CHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHEEECHHHHHHHHHHHCCCCCC
KDINVALRIIDKLDKIGKERVSEELQKELGLNNNQVENLLEYTSIKQQGESESFFRQISF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHH
MEEWNDSIKKGIEDLKMLYEVLSSLQMDRDTYRVNFSIARGLGYYTGIVYETTLNALKSI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHCCHHHHHHHHHHHHHHHHHH
GSVCSGGRYDNLTRTFSKEKMSGVGASIGIDRLLAALEELELLQKRATSARALIVCMDRL
HHHHCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YFGYAHLLAESFRCSEIPIEVYPEASKLKKQFTYAHLKGYEFVVVIGENEFNTKTLTLKN
HHHHHHHHHHHCCCCCCCEEECCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCEEEEEEC
MTSGVQIDSISFLKALALIQEE
CCCCCEEHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ALVTPRTLSGFKDRLPKEALAKAHLLNKVSNVFMNFGFVPIETPHLEYADVLIKQGSDE
CCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCEECCCCCCHHHHHHHHCCCHH
IQKELYRFKDHGGRDVALRFDLTVPLARFVSQYKNEVGLPFKRYAIGNVFRGERAQRGRY
HHHHHHHHHCCCCCEEEEEEECHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCHHHCCCC
REFTQCDFDFIGSDSISCDAEILQVIYASLMKLGIEEFTIWLNHRSILNGICEYCGITQE
CHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHEEECHHHHHHHHHHHCCCCCC
KDINVALRIIDKLDKIGKERVSEELQKELGLNNNQVENLLEYTSIKQQGESESFFRQISF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHH
MEEWNDSIKKGIEDLKMLYEVLSSLQMDRDTYRVNFSIARGLGYYTGIVYETTLNALKSI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHCCHHHHHHHHHHHHHHHHHH
GSVCSGGRYDNLTRTFSKEKMSGVGASIGIDRLLAALEELELLQKRATSARALIVCMDRL
HHHHCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YFGYAHLLAESFRCSEIPIEVYPEASKLKKQFTYAHLKGYEFVVVIGENEFNTKTLTLKN
HHHHHHHHHHHCCCCCCCEEECCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCEEEEEEC
MTSGVQIDSISFLKALALIQEE
CCCCCEEHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12810954