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The map label for this gene is ligA

Identifier: 32267302

GI number: 32267302

Start: 1725150

End: 1727102

Strand: Direct

Name: ligA

Synonym: HH1803

Alternate gene names: 32267302

Gene position: 1725150-1727102 (Clockwise)

Preceding gene: 32267298

Following gene: 32267308

Centisome position: 95.89

GC content: 36.25

Gene sequence:

>1953_bases
ATGACAAGTCAAGAATATTACACACAAGTGCAGATTCTCAAAAAGATGGCATATCACTATTATGTGCTTGATGAACCTAT
TGCAACTGATGAGCAATATGATAGCCTCTATCATCAGATAGTACGTTTTGAAGAAGAAAATCCTCATCTTATTGATGCAA
GTTCGCCAACACAACGTGTAGGTGATGTGCCCTTAGAATCTTTTAGTAAAAATACGCATTTGGAGCGTATGTGGAGTCTA
GAAGATGTTTTTAATTATGATGAACTAGTAGAATGGGTGCAAAGAATCTATAAAAGCTACCCTCAAGCTACTTTTACTTG
CTCACCTAAATTTGATGGCGCAAGTCTCAATCTCCTTTATCAAGAAGGCAAGCTAGTGAGTGCTACGACACGAGGAGATG
GTGTTATTGGGGAGCTTGTAACGCATAATGCAAAGACGATTCAGAGTATTCCTCTTGAAATTAATTATAAAGAGGAAATT
GAGATACGCGGTGAAGTAGTCATAGCCAAAGAGGATTTTGAATATATCAATCAAGATCGTTTAAGCGAAAACTTAAGCCT
TTTTGCAAATCCTCGTAATGCAGCAGCAGGGAGTTTGAGACAGCTTGATGCAAAAATTACTGCTAAACGCAAACTTCGTT
TTATGCCTTGGGGCATTGGGGCAGGTTTGATACGATTTGAGAGCTTTTATGAAGCTTTTAATACAATTACACGACTTGGT
TTTGCTCCTGTGCCTTTTTTGAGTTATTGCGAGAGTATAGAATCCATACAAAATGCTTATAATGTGCTTTTTTCTCAACG
CAATAATTATCCCATAATGCTTGATGGTATGGTGATTATGCTTGATAAAATTGCCTCTCAACAACAACTTGGTTGGACGA
TTAAATCTCCTCGTTTTGCTTGTGCTTATAAGTTTCCCGCAGTGGAAAAGAGCTCCAAGATTCTATCTGTAAGCCTACAA
GTTGGACGCACAGGTATTATTACACCTGTGGCAGAACTAAAGCCTGTGGAGATTGAGGGAGCGATGATTTCACGTGCAAC
TTTACACAACTTTAGTGAAATTGAGCGCAAAGATATTCGTTTGGGCGATGAAGTGATTATTATTCGGAGTGGAGATGTGA
TTCCTAAAATCATTAAACCGCTTGTGGCTTTGCGTGATGGTACTCAAAAAGTTATTTCTAAACCTACGCATTGTCCAGTC
TGTGGAGAAGAACTTTTACTTGAAGATATATTCATCAAATGTCAGAATCTAAGCTGTGAAGCGCGTGTAATAGAATCCAT
TATCCATTTTGCCTCAAAAAAAGCGCTTAATATTGATGGTTTAGGTGAAAAAATCGTAATACAACTCTATGAAAATGCCT
TTGTAAGAAATATTAAAGATATTTATGCTTTAAGCATACAACAATTGCTCACACTTGAAGGGTGGCAAGAAAAGAGGGCA
AATAATCTTATTTATGCTATACAAAATACTATAGGTGTAGAGTTATGGCGTTTTATTAATGCACTTGGCATTGAACATAT
TGGTGAAGGCGCAAGTAAAAAGCTTGCTCAAAAATTTGGATTAAATGTGTTTGAACTTGAACTTTCAGAAATATTGAGCG
TTGATGGATTTGGGGAGGAAATGGCATATTCGCTTGTGGAATTTAATCACGCTAACAAAACGCTTATTGCTGAGCTGCTT
TGTATTATCAAGCCTAAAGTAGAGTTATTAGAAATTGATAACAATAATGTTTTCTTCAATAAAACTATTGTGCTTACAGG
CACACTAAGTCAGCCAAGAGATAGAATTATTAAACTTTTAGAATCTAAGGGTGCAAAAATAAGCTCAAGTGTAAGTAAAA
AAACCGATTTTGTTATTTATGGAGAAAAGGCAGGGAGCAAGCTAGAAAAAGCACAATCTTTAAATATTACCACATTAAAC
GAAGAAGAATTTCTTGCACAAATCAATAAGTGA

Upstream 100 bases:

>100_bases
ATAAGAAGAAATACAGAAATGTCGCTTAAATAATGGTATGATTCTATCATAAAATACATAATATAAGCACTTAAACTTCA
AATTTTAAAGGTAAATCGTT

Downstream 100 bases:

>100_bases
AAGAATTTATAGTTCTAGTAATGATACAGGCACTTTCAATACACTTTTTTTAATAGCATAAGTGTAAGGTGTAGGCACAT
CAGGTGTGAGTTCTAAACCT

Product: NAD-dependent DNA ligase LigA

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 650; Mature: 649

Protein sequence:

>650_residues
MTSQEYYTQVQILKKMAYHYYVLDEPIATDEQYDSLYHQIVRFEEENPHLIDASSPTQRVGDVPLESFSKNTHLERMWSL
EDVFNYDELVEWVQRIYKSYPQATFTCSPKFDGASLNLLYQEGKLVSATTRGDGVIGELVTHNAKTIQSIPLEINYKEEI
EIRGEVVIAKEDFEYINQDRLSENLSLFANPRNAAAGSLRQLDAKITAKRKLRFMPWGIGAGLIRFESFYEAFNTITRLG
FAPVPFLSYCESIESIQNAYNVLFSQRNNYPIMLDGMVIMLDKIASQQQLGWTIKSPRFACAYKFPAVEKSSKILSVSLQ
VGRTGIITPVAELKPVEIEGAMISRATLHNFSEIERKDIRLGDEVIIIRSGDVIPKIIKPLVALRDGTQKVISKPTHCPV
CGEELLLEDIFIKCQNLSCEARVIESIIHFASKKALNIDGLGEKIVIQLYENAFVRNIKDIYALSIQQLLTLEGWQEKRA
NNLIYAIQNTIGVELWRFINALGIEHIGEGASKKLAQKFGLNVFELELSEILSVDGFGEEMAYSLVEFNHANKTLIAELL
CIIKPKVELLEIDNNNVFFNKTIVLTGTLSQPRDRIIKLLESKGAKISSSVSKKTDFVIYGEKAGSKLEKAQSLNITTLN
EEEFLAQINK

Sequences:

>Translated_650_residues
MTSQEYYTQVQILKKMAYHYYVLDEPIATDEQYDSLYHQIVRFEEENPHLIDASSPTQRVGDVPLESFSKNTHLERMWSL
EDVFNYDELVEWVQRIYKSYPQATFTCSPKFDGASLNLLYQEGKLVSATTRGDGVIGELVTHNAKTIQSIPLEINYKEEI
EIRGEVVIAKEDFEYINQDRLSENLSLFANPRNAAAGSLRQLDAKITAKRKLRFMPWGIGAGLIRFESFYEAFNTITRLG
FAPVPFLSYCESIESIQNAYNVLFSQRNNYPIMLDGMVIMLDKIASQQQLGWTIKSPRFACAYKFPAVEKSSKILSVSLQ
VGRTGIITPVAELKPVEIEGAMISRATLHNFSEIERKDIRLGDEVIIIRSGDVIPKIIKPLVALRDGTQKVISKPTHCPV
CGEELLLEDIFIKCQNLSCEARVIESIIHFASKKALNIDGLGEKIVIQLYENAFVRNIKDIYALSIQQLLTLEGWQEKRA
NNLIYAIQNTIGVELWRFINALGIEHIGEGASKKLAQKFGLNVFELELSEILSVDGFGEEMAYSLVEFNHANKTLIAELL
CIIKPKVELLEIDNNNVFFNKTIVLTGTLSQPRDRIIKLLESKGAKISSSVSKKTDFVIYGEKAGSKLEKAQSLNITTLN
EEEFLAQINK
>Mature_649_residues
TSQEYYTQVQILKKMAYHYYVLDEPIATDEQYDSLYHQIVRFEEENPHLIDASSPTQRVGDVPLESFSKNTHLERMWSLE
DVFNYDELVEWVQRIYKSYPQATFTCSPKFDGASLNLLYQEGKLVSATTRGDGVIGELVTHNAKTIQSIPLEINYKEEIE
IRGEVVIAKEDFEYINQDRLSENLSLFANPRNAAAGSLRQLDAKITAKRKLRFMPWGIGAGLIRFESFYEAFNTITRLGF
APVPFLSYCESIESIQNAYNVLFSQRNNYPIMLDGMVIMLDKIASQQQLGWTIKSPRFACAYKFPAVEKSSKILSVSLQV
GRTGIITPVAELKPVEIEGAMISRATLHNFSEIERKDIRLGDEVIIIRSGDVIPKIIKPLVALRDGTQKVISKPTHCPVC
GEELLLEDIFIKCQNLSCEARVIESIIHFASKKALNIDGLGEKIVIQLYENAFVRNIKDIYALSIQQLLTLEGWQEKRAN
NLIYAIQNTIGVELWRFINALGIEHIGEGASKKLAQKFGLNVFELELSEILSVDGFGEEMAYSLVEFNHANKTLIAELLC
IIKPKVELLEIDNNNVFFNKTIVLTGTLSQPRDRIIKLLESKGAKISSSVSKKTDFVIYGEKAGSKLEKAQSLNITTLNE
EEFLAQINK

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Escherichia coli, GI1788750, Length=654, Percent_Identity=40.3669724770642, Blast_Score=455, Evalue=1e-129,
Organism=Escherichia coli, GI87082305, Length=409, Percent_Identity=23.7163814180929, Blast_Score=97, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_HELHP (Q7VF74)

Other databases:

- EMBL:   AE017125
- RefSeq:   NP_861334.1
- HSSP:   O87703
- ProteinModelPortal:   Q7VF74
- GeneID:   1492350
- GenomeReviews:   AE017125_GR
- KEGG:   hhe:HH1803
- NMPDR:   fig|235279.1.peg.1803
- HOGENOM:   HBG620317
- OMA:   HNQDYID
- ProtClustDB:   PRK07956
- BioCyc:   HHEP235279:HH_1803-MONOMER
- BRENDA:   6.5.1.2
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 73555; Mature: 73423

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 110-110 BINDING 131-131 BINDING 165-165 BINDING 304-304

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSQEYYTQVQILKKMAYHYYVLDEPIATDEQYDSLYHQIVRFEEENPHLIDASSPTQRV
CCCHHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHHHCCCCCCCEEECCCCHHHH
GDVPLESFSKNTHLERMWSLEDVFNYDELVEWVQRIYKSYPQATFTCSPKFDGASLNLLY
CCCCHHHHCCCCHHHHHHCHHHHCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCEEEEEE
QEGKLVSATTRGDGVIGELVTHNAKTIQSIPLEINYKEEIEIRGEVVIAKEDFEYINQDR
ECCEEEEEECCCCCCHHHHHHCCCHHHEECCEEECCCCCEEEEEEEEEEECHHHHHCHHH
LSENLSLFANPRNAAAGSLRQLDAKITAKRKLRFMPWGIGAGLIRFESFYEAFNTITRLG
HCCCCEEEECCCCHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHHHHHHCC
FAPVPFLSYCESIESIQNAYNVLFSQRNNYPIMLDGMVIMLDKIASQQQLGWTIKSPRFA
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCHHHHHHHHCCHHHCCEEECCCCEE
CAYKFPAVEKSSKILSVSLQVGRTGIITPVAELKPVEIEGAMISRATLHNFSEIERKDIR
EEEECCCCCCCCCEEEEEEEECCCCCCCCHHHCCCEEECCHHHHHHHHHHHHHHHHHHCC
LGDEVIIIRSGDVIPKIIKPLVALRDGTQKVISKPTHCPVCGEELLLEDIFIKCQNLSCE
CCCEEEEEECCCCHHHHHHHHHHHCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCHH
ARVIESIIHFASKKALNIDGLGEKIVIQLYENAFVRNIKDIYALSIQQLLTLEGWQEKRA
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHC
NNLIYAIQNTIGVELWRFINALGIEHIGEGASKKLAQKFGLNVFELELSEILSVDGFGEE
CCEEEEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCEEEEEHHHHHCCCCCCHH
MAYSLVEFNHANKTLIAELLCIIKPKVELLEIDNNNVFFNKTIVLTGTLSQPRDRIIKLL
HHHHHHHHCCCCHHHHHHHHHHHCCCEEEEEECCCEEEEEEEEEEEECCCCHHHHHHHHH
ESKGAKISSSVSKKTDFVIYGEKAGSKLEKAQSLNITTLNEEEFLAQINK
HHCCCCCHHHHCCCCCEEEECCCCCCHHHHHCCCCEEEECHHHHHHHCCC
>Mature Secondary Structure 
TSQEYYTQVQILKKMAYHYYVLDEPIATDEQYDSLYHQIVRFEEENPHLIDASSPTQRV
CCHHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHHHCCCCCCCEEECCCCHHHH
GDVPLESFSKNTHLERMWSLEDVFNYDELVEWVQRIYKSYPQATFTCSPKFDGASLNLLY
CCCCHHHHCCCCHHHHHHCHHHHCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCEEEEEE
QEGKLVSATTRGDGVIGELVTHNAKTIQSIPLEINYKEEIEIRGEVVIAKEDFEYINQDR
ECCEEEEEECCCCCCHHHHHHCCCHHHEECCEEECCCCCEEEEEEEEEEECHHHHHCHHH
LSENLSLFANPRNAAAGSLRQLDAKITAKRKLRFMPWGIGAGLIRFESFYEAFNTITRLG
HCCCCEEEECCCCHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHHHHHHCC
FAPVPFLSYCESIESIQNAYNVLFSQRNNYPIMLDGMVIMLDKIASQQQLGWTIKSPRFA
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCHHHHHHHHCCHHHCCEEECCCCEE
CAYKFPAVEKSSKILSVSLQVGRTGIITPVAELKPVEIEGAMISRATLHNFSEIERKDIR
EEEECCCCCCCCCEEEEEEEECCCCCCCCHHHCCCEEECCHHHHHHHHHHHHHHHHHHCC
LGDEVIIIRSGDVIPKIIKPLVALRDGTQKVISKPTHCPVCGEELLLEDIFIKCQNLSCE
CCCEEEEEECCCCHHHHHHHHHHHCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCHH
ARVIESIIHFASKKALNIDGLGEKIVIQLYENAFVRNIKDIYALSIQQLLTLEGWQEKRA
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHC
NNLIYAIQNTIGVELWRFINALGIEHIGEGASKKLAQKFGLNVFELELSEILSVDGFGEE
CCEEEEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCEEEEEHHHHHCCCCCCHH
MAYSLVEFNHANKTLIAELLCIIKPKVELLEIDNNNVFFNKTIVLTGTLSQPRDRIIKLL
HHHHHHHHCCCCHHHHHHHHHHHCCCEEEEEECCCEEEEEEEEEEEECCCCHHHHHHHHH
ESKGAKISSSVSKKTDFVIYGEKAGSKLEKAQSLNITTLNEEEFLAQINK
HHCCCCCHHHHCCCCCEEEECCCCCCHHHHHCCCCEEEECHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12810954