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The map label for this gene is dapA

Identifier: 32267125

GI number: 32267125

Start: 1549181

End: 1550062

Strand: Direct

Name: dapA

Synonym: HH1626

Alternate gene names: 32267125

Gene position: 1549181-1550062 (Clockwise)

Preceding gene: 32267124

Following gene: 32267126

Centisome position: 86.11

GC content: 37.87

Gene sequence:

>882_bases
ATGGTTTTTGGGGCAATGAGTGCATTAATCACACCATTTATAAATCATAAGGTTGATTTTGAAACATATGAAACACTCAT
TAAGCGGCAAATTACCTATGGTATGGACGCTTGTGTGCCAGTAGGCACGACAGGAGAATCGGCTACACTTTCGCATAAAG
AACATATGGAATGTATTGAAGTTGCCGTAGCGGTATGTAGGGGTAGTAAAGTAAAAGTGCTTGCAGGTGCAGGGAGTAAT
TCTACAAGCGAGGCAAAAGAACTTGCACTTTTTGCCCAAAAATGCGGTGCAGATGCGCTATTATGTGTTACACCTTATTA
TAATAAACCTACACAAGAGGGTTTGTTTGAGCATTATAAGGCTGTGGCTAATGCGGTGGAGATTCCTGTAATGCTTTATA
ATGTTCCATCACGCACAGGAGTAAGTATAGAGATAGAAACTGCTAAACGTCTGCGTGATGCTTGTAAAAATATTTATGCA
ATTAAGGAGGCAGCTGGACTTATGGAAAGAGTAGTGGCTTTTGGTGCAGAAGTGCCTAATCTTGCACTTTTAAGTGGAGA
AGATGTAATCAATTACCCCATACTTGCAAATAATGGCAAAGGCGTTATATCTGTAACCGGTAATCTTTTCCCACAAGAGA
TTGCTGATTTAACGCATTATGCTTTACAAGGTAATATGGAAAAAAGCTATGAAATTAATACAAGGCTTTATGAGATGAAT
AGGATACTTTTTTGTGAAAGTAATCCTGTGCCCATTAAAGCGGCAATGTTTTTAGCAGGATTATTAAAAAACTTGGAATA
TCGTTTGCCATTAGTGCCACCTTCTAAGGAAAATATGGCTAAAATACAACAAATCTTAGAAAAATACGAGGTGAAAGAAT
GA

Upstream 100 bases:

>100_bases
TAAATACTCTTAACTTAGATAAGATTCAGCAGGTAGCAAATAAATATTTTGTAGAAGATACACTAAGTGTGGTGATTTTA
AAGGACAAGGGGAGGTGATT

Downstream 100 bases:

>100_bases
AAAATGCTCAAGATTCTACAACACATTTTATGAAAGGCAAAACACTTGTTATTAGCGGTGCGACACGAGGTATTGGCAAG
GCAATTTTATATAAATTTGC

Product: dihydrodipicolinate synthase

Products: NA

Alternate protein names: DHDPS

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MVFGAMSALITPFINHKVDFETYETLIKRQITYGMDACVPVGTTGESATLSHKEHMECIEVAVAVCRGSKVKVLAGAGSN
STSEAKELALFAQKCGADALLCVTPYYNKPTQEGLFEHYKAVANAVEIPVMLYNVPSRTGVSIEIETAKRLRDACKNIYA
IKEAAGLMERVVAFGAEVPNLALLSGEDVINYPILANNGKGVISVTGNLFPQEIADLTHYALQGNMEKSYEINTRLYEMN
RILFCESNPVPIKAAMFLAGLLKNLEYRLPLVPPSKENMAKIQQILEKYEVKE

Sequences:

>Translated_293_residues
MVFGAMSALITPFINHKVDFETYETLIKRQITYGMDACVPVGTTGESATLSHKEHMECIEVAVAVCRGSKVKVLAGAGSN
STSEAKELALFAQKCGADALLCVTPYYNKPTQEGLFEHYKAVANAVEIPVMLYNVPSRTGVSIEIETAKRLRDACKNIYA
IKEAAGLMERVVAFGAEVPNLALLSGEDVINYPILANNGKGVISVTGNLFPQEIADLTHYALQGNMEKSYEINTRLYEMN
RILFCESNPVPIKAAMFLAGLLKNLEYRLPLVPPSKENMAKIQQILEKYEVKE
>Mature_293_residues
MVFGAMSALITPFINHKVDFETYETLIKRQITYGMDACVPVGTTGESATLSHKEHMECIEVAVAVCRGSKVKVLAGAGSN
STSEAKELALFAQKCGADALLCVTPYYNKPTQEGLFEHYKAVANAVEIPVMLYNVPSRTGVSIEIETAKRLRDACKNIYA
IKEAAGLMERVVAFGAEVPNLALLSGEDVINYPILANNGKGVISVTGNLFPQEIADLTHYALQGNMEKSYEINTRLYEMN
RILFCESNPVPIKAAMFLAGLLKNLEYRLPLVPPSKENMAKIQQILEKYEVKE

Specific function: Biosynthesis of diaminopimelate and lysine from aspartate semialdehyde; first step. [C]

COG id: COG0329

COG function: function code EM; Dihydrodipicolinate synthase/N-acetylneuraminate lyase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHDPS family

Homologues:

Organism=Homo sapiens, GI31543060, Length=244, Percent_Identity=25.4098360655738, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI13540533, Length=150, Percent_Identity=33.3333333333333, Blast_Score=66, Evalue=5e-11,
Organism=Escherichia coli, GI1788823, Length=289, Percent_Identity=38.4083044982699, Blast_Score=191, Evalue=6e-50,
Organism=Escherichia coli, GI87082415, Length=263, Percent_Identity=30.7984790874525, Blast_Score=107, Evalue=9e-25,
Organism=Escherichia coli, GI1786463, Length=252, Percent_Identity=30.1587301587302, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1789620, Length=242, Percent_Identity=27.6859504132231, Blast_Score=101, Evalue=7e-23,

Paralogues:

None

Copy number: 840 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): DAPA_HELHP (Q7VFP9)

Other databases:

- EMBL:   AE017125
- RefSeq:   NP_861157.1
- ProteinModelPortal:   Q7VFP9
- SMR:   Q7VFP9
- GeneID:   1492173
- GenomeReviews:   AE017125_GR
- KEGG:   hhe:HH1626
- NMPDR:   fig|235279.1.peg.1626
- HOGENOM:   HBG358848
- OMA:   GMYRHFK
- ProtClustDB:   PRK03170
- BioCyc:   HHEP235279:HH_1626-MONOMER
- BRENDA:   4.2.1.52
- GO:   GO:0005737
- HAMAP:   MF_00418
- InterPro:   IPR013785
- InterPro:   IPR005263
- InterPro:   IPR002220
- InterPro:   IPR020625
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR12128
- PRINTS:   PR00146
- TIGRFAMs:   TIGR00674

Pfam domain/function: PF00701 DHDPS

EC number: =4.2.1.52

Molecular weight: Translated: 32190; Mature: 32190

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS00665 DHDPS_1; PS00666 DHDPS_2

Important sites: ACT_SITE 162-162 BINDING 106-106

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVFGAMSALITPFINHKVDFETYETLIKRQITYGMDACVPVGTTGESATLSHKEHMECIE
CCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCHHHHHHHHH
VAVAVCRGSKVKVLAGAGSNSTSEAKELALFAQKCGADALLCVTPYYNKPTQEGLFEHYK
HHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHH
AVANAVEIPVMLYNVPSRTGVSIEIETAKRLRDACKNIYAIKEAAGLMERVVAFGAEVPN
HHHHHHHCCEEEEECCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
LALLSGEDVINYPILANNGKGVISVTGNLFPQEIADLTHYALQGNMEKSYEINTRLYEMN
EEEECCCCEEECCEEECCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCEEHHHEEEEEC
RILFCESNPVPIKAAMFLAGLLKNLEYRLPLVPPSKENMAKIQQILEKYEVKE
EEEEECCCCCCHHHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MVFGAMSALITPFINHKVDFETYETLIKRQITYGMDACVPVGTTGESATLSHKEHMECIE
CCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCHHHHHHHHH
VAVAVCRGSKVKVLAGAGSNSTSEAKELALFAQKCGADALLCVTPYYNKPTQEGLFEHYK
HHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHH
AVANAVEIPVMLYNVPSRTGVSIEIETAKRLRDACKNIYAIKEAAGLMERVVAFGAEVPN
HHHHHHHCCEEEEECCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
LALLSGEDVINYPILANNGKGVISVTGNLFPQEIADLTHYALQGNMEKSYEINTRLYEMN
EEEECCCCEEECCEEECCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCEEHHHEEEEEC
RILFCESNPVPIKAAMFLAGLLKNLEYRLPLVPPSKENMAKIQQILEKYEVKE
EEEEECCCCCCHHHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12810954