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The map label for this gene is ecaIM [H]

Identifier: 32266944

GI number: 32266944

Start: 1394487

End: 1394813

Strand: Direct

Name: ecaIM [H]

Synonym: HH1445

Alternate gene names: 32266944

Gene position: 1394487-1394813 (Clockwise)

Preceding gene: 32266943

Following gene: 32266945

Centisome position: 77.51

GC content: 32.11

Gene sequence:

>327_bases
GTGCAATCAAAAGAAACGAATCGCCAAATAGGACCGCTTGATGATAAGGTGGGACACTATGTCAAAATCTTATGTGATGA
GATTTTTGGAAGGGAGCATTTTATCAATGATATTACGCGCATTAAATGTAATCCTAAAAACTTTAAACGCAAAGCCTATG
GGAATGTCAAAGATAGAATCTTATTTTATGTAAAAAGCAATCAATATGTTTGGAATGAAGTTTATGAGGAAATCACAAAA
AATGATTTACATAAACGATTTAAAAAGCAAGATAAAAAAGCTTTTATACGATTATACCGCTTCACTCACCCACTGCTAAA
TAAGTGA

Upstream 100 bases:

>100_bases
TTTAGTGTCAATGAGCAAGAAAAAATTATAGAATCCTTACTTGATTTTGATTTGTTTCTTATTAAAGATTTTTATATTGC
TTATCTAAGGCGCGACAAGA

Downstream 100 bases:

>100_bases
TTATCCGTTTAGAACAAATGAGCGCAAATAGTTAAGGAGCAATTAAAAAAAGATTAAAAATGATAAATTTTTAATAAAAA
TAAAAATTTTTGCATTATTA

Product: hypothetical protein

Products: NA

Alternate protein names: M.EcaI; Adenine-specific methyltransferase EcaI [H]

Number of amino acids: Translated: 108; Mature: 108

Protein sequence:

>108_residues
MQSKETNRQIGPLDDKVGHYVKILCDEIFGREHFINDITRIKCNPKNFKRKAYGNVKDRILFYVKSNQYVWNEVYEEITK
NDLHKRFKKQDKKAFIRLYRFTHPLLNK

Sequences:

>Translated_108_residues
MQSKETNRQIGPLDDKVGHYVKILCDEIFGREHFINDITRIKCNPKNFKRKAYGNVKDRILFYVKSNQYVWNEVYEEITK
NDLHKRFKKQDKKAFIRLYRFTHPLLNK
>Mature_108_residues
MQSKETNRQIGPLDDKVGHYVKILCDEIFGREHFINDITRIKCNPKNFKRKAYGNVKDRILFYVKSNQYVWNEVYEEITK
NDLHKRFKKQDKKAFIRLYRFTHPLLNK

Specific function: This methylase recognizes the double-stranded sequence GGTNACC, causes specific methylation on A-5 on both strands and protects the DNA from cleavage by the EcaI endonuclease [H]

COG id: COG2189

COG function: function code L; Adenine specific DNA methylase Mod

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002295
- InterPro:   IPR002941
- InterPro:   IPR002052 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 13165; Mature: 13165

Theoretical pI: Translated: 10.35; Mature: 10.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSKETNRQIGPLDDKVGHYVKILCDEIFGREHFINDITRIKCNPKNFKRKAYGNVKDRI
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHEECCHHHHHHHHCCCCCHHE
LFYVKSNQYVWNEVYEEITKNDLHKRFKKQDKKAFIRLYRFTHPLLNK
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQSKETNRQIGPLDDKVGHYVKILCDEIFGREHFINDITRIKCNPKNFKRKAYGNVKDRI
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHEECCHHHHHHHHCCCCCHHE
LFYVKSNQYVWNEVYEEITKNDLHKRFKKQDKKAFIRLYRFTHPLLNK
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2183182 [H]