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The map label for this gene is gatA

Identifier: 32266200

GI number: 32266200

Start: 680733

End: 682076

Strand: Direct

Name: gatA

Synonym: HH0701

Alternate gene names: 32266200

Gene position: 680733-682076 (Clockwise)

Preceding gene: 32266199

Following gene: 32266201

Centisome position: 37.84

GC content: 39.29

Gene sequence:

>1344_bases
ATGATAACACTCAAAGAAGCTCTAAACAAATCCGAAAATGAACTCAAAGAGATAAAAACTCTCTTGCGCCAACGCGTCAA
GGAAAACATAGAACTCAATGCTTATATTGGCGAAATTAACGAAAGTGGAAGTGGTGTGCCAATTCTCATAAAAGACAATA
TCAATGTTAAAGGTTGGGAAATCACTTGTGCGAGTAAAATTTTAAAAGGCTATGTTTCTCCTTATAATGCAAGTGTTATC
ACAAAATTACACGAAAACAAAATGTGTGCTTTTGGACGCGCCAATATGGACGAATTTGCAATGGGAAGCACAACAGAATC
TAGCTGTTATGGGAGAGTGAAAAATCCTCTTGATACTTCGCGTGTGCCAGGTGGAAGCAGTGGGGGAAGTGCGGCAGCTG
TAGCGGCTGGACTTGCTATTGCCTCACTTGGAAGCGATACGGGTGGTTCAATACGGCAGCCTGCTGGATTTTGCGGTTGT
GTAGGGTTAAAACCAAGCTATGGAAGAGTAAGTCGTTATGGGTTAATTGCATATAGCTCAAGTTTAGACCAAATTGGACC
TATTACGCAAAATGTTGAAGATGCTGCACTTTTGCTTGATGCCATAAGCGGATATGATAAGCAAGATTCTACAAGTGCGA
ATCTCCCATCACTCAACACATTTAAAGACCTTGACCCAAATGCACGTTACAAGGTTGCTATTTTAAAAGATTTCCTTAAA
GATGCCACCCCCCAAATACAAGAAGCCTATAATCACACTATTAAAATTTTAGAATCTATGGGGCATACCATTGTTGAAAA
ATCTATGCTTGATACACGCTATCATATCTCGGCATACTATATTATCTGTACTGCAGAGGCTAGCTCTAACCTTGCGCGTT
TTGATGGAATCCGCTATGGCACAAGAAGCGAATCAAAAAATCTCAAAGATGTATATCTTAATACACGCAGTGCATATTTT
GGCGAAGAAGTAAAGCGCCGCATTTTGCTTGGAAGCTTCGTGCTAAGCAGCGGTTATTATGATGCGTATTATCTCAAAGC
ACAGCAAGTGCGAAAGAAAATCTGTGCAGATTACGAATCTATCTTTAATGAGTGCGATAGTATTCTCTTGCCTATTGCCC
CAAGTGTAGCCCCTAAATTTGGCTCAACGCATTCGTCCTTAGAAATGTATTTAAGTGATATTTATACTATTGGTGTCAAT
CTAGCAGGATTACCAGCCTTATGTATGCCTGTAAGCAAGGACAAAAATGGTTTAAGCATTGGTATGCAGTTTATTGGTGC
AAAATTCAAAGAACAGAACATTCTCAATATTGCCTATGGATTAGAAAAAGAAATAAACAATTAA

Upstream 100 bases:

>100_bases
TTAAAAAAGATTTATAAGCAATAACAAAATTTATATTATAGTTATTTTAAAGCAAGAAGATTCTATAATCACACTTCCTT
AACACAATAGGAGACAATAA

Downstream 100 bases:

>100_bases
TTTTATAAAGGAGTAAAATATGAAAATTAAACAAAAAGCTCTCACATTTGAAGATGTTTTACTTATCCCTGCATATTCCG
AAATTCTCCCTCAAGAAGTT

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: monocarboxylate; NH3

Alternate protein names: Glu-ADT subunit A

Number of amino acids: Translated: 447; Mature: 447

Protein sequence:

>447_residues
MITLKEALNKSENELKEIKTLLRQRVKENIELNAYIGEINESGSGVPILIKDNINVKGWEITCASKILKGYVSPYNASVI
TKLHENKMCAFGRANMDEFAMGSTTESSCYGRVKNPLDTSRVPGGSSGGSAAAVAAGLAIASLGSDTGGSIRQPAGFCGC
VGLKPSYGRVSRYGLIAYSSSLDQIGPITQNVEDAALLLDAISGYDKQDSTSANLPSLNTFKDLDPNARYKVAILKDFLK
DATPQIQEAYNHTIKILESMGHTIVEKSMLDTRYHISAYYIICTAEASSNLARFDGIRYGTRSESKNLKDVYLNTRSAYF
GEEVKRRILLGSFVLSSGYYDAYYLKAQQVRKKICADYESIFNECDSILLPIAPSVAPKFGSTHSSLEMYLSDIYTIGVN
LAGLPALCMPVSKDKNGLSIGMQFIGAKFKEQNILNIAYGLEKEINN

Sequences:

>Translated_447_residues
MITLKEALNKSENELKEIKTLLRQRVKENIELNAYIGEINESGSGVPILIKDNINVKGWEITCASKILKGYVSPYNASVI
TKLHENKMCAFGRANMDEFAMGSTTESSCYGRVKNPLDTSRVPGGSSGGSAAAVAAGLAIASLGSDTGGSIRQPAGFCGC
VGLKPSYGRVSRYGLIAYSSSLDQIGPITQNVEDAALLLDAISGYDKQDSTSANLPSLNTFKDLDPNARYKVAILKDFLK
DATPQIQEAYNHTIKILESMGHTIVEKSMLDTRYHISAYYIICTAEASSNLARFDGIRYGTRSESKNLKDVYLNTRSAYF
GEEVKRRILLGSFVLSSGYYDAYYLKAQQVRKKICADYESIFNECDSILLPIAPSVAPKFGSTHSSLEMYLSDIYTIGVN
LAGLPALCMPVSKDKNGLSIGMQFIGAKFKEQNILNIAYGLEKEINN
>Mature_447_residues
MITLKEALNKSENELKEIKTLLRQRVKENIELNAYIGEINESGSGVPILIKDNINVKGWEITCASKILKGYVSPYNASVI
TKLHENKMCAFGRANMDEFAMGSTTESSCYGRVKNPLDTSRVPGGSSGGSAAAVAAGLAIASLGSDTGGSIRQPAGFCGC
VGLKPSYGRVSRYGLIAYSSSLDQIGPITQNVEDAALLLDAISGYDKQDSTSANLPSLNTFKDLDPNARYKVAILKDFLK
DATPQIQEAYNHTIKILESMGHTIVEKSMLDTRYHISAYYIICTAEASSNLARFDGIRYGTRSESKNLKDVYLNTRSAYF
GEEVKRRILLGSFVLSSGYYDAYYLKAQQVRKKICADYESIFNECDSILLPIAPSVAPKFGSTHSSLEMYLSDIYTIGVN
LAGLPALCMPVSKDKNGLSIGMQFIGAKFKEQNILNIAYGLEKEINN

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family

Homologues:

Organism=Homo sapiens, GI222831590, Length=430, Percent_Identity=38.6046511627907, Blast_Score=288, Evalue=8e-78,
Organism=Homo sapiens, GI195972892, Length=410, Percent_Identity=23.4146341463415, Blast_Score=98, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17543272, Length=441, Percent_Identity=33.5600907029478, Blast_Score=241, Evalue=8e-64,
Organism=Caenorhabditis elegans, GI17556264, Length=248, Percent_Identity=32.6612903225806, Blast_Score=111, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI17537465, Length=428, Percent_Identity=25.7009345794392, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI71990152, Length=241, Percent_Identity=31.5352697095436, Blast_Score=96, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17538252, Length=234, Percent_Identity=28.6324786324786, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17556276, Length=276, Percent_Identity=26.4492753623188, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17556278, Length=276, Percent_Identity=26.4492753623188, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17538254, Length=234, Percent_Identity=25.6410256410256, Blast_Score=65, Evalue=9e-11,
Organism=Saccharomyces cerevisiae, GI6323950, Length=424, Percent_Identity=35.377358490566, Blast_Score=222, Evalue=9e-59,
Organism=Saccharomyces cerevisiae, GI6319685, Length=437, Percent_Identity=26.3157894736842, Blast_Score=87, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24648113, Length=443, Percent_Identity=35.8916478555305, Blast_Score=241, Evalue=5e-64,
Organism=Drosophila melanogaster, GI24644968, Length=419, Percent_Identity=26.491646778043, Blast_Score=112, Evalue=6e-25,
Organism=Drosophila melanogaster, GI24652985, Length=423, Percent_Identity=26.7139479905437, Blast_Score=108, Evalue=5e-24,
Organism=Drosophila melanogaster, GI19922090, Length=423, Percent_Identity=26.7139479905437, Blast_Score=108, Evalue=5e-24,
Organism=Drosophila melanogaster, GI24652981, Length=423, Percent_Identity=26.7139479905437, Blast_Score=108, Evalue=5e-24,
Organism=Drosophila melanogaster, GI24652983, Length=423, Percent_Identity=26.7139479905437, Blast_Score=108, Evalue=5e-24,
Organism=Drosophila melanogaster, GI161078093, Length=421, Percent_Identity=26.3657957244656, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24648435, Length=417, Percent_Identity=24.220623501199, Blast_Score=86, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24648437, Length=417, Percent_Identity=24.220623501199, Blast_Score=86, Evalue=6e-17,
Organism=Drosophila melanogaster, GI45550774, Length=417, Percent_Identity=24.220623501199, Blast_Score=86, Evalue=7e-17,
Organism=Drosophila melanogaster, GI24648441, Length=417, Percent_Identity=24.220623501199, Blast_Score=86, Evalue=8e-17,
Organism=Drosophila melanogaster, GI24648439, Length=417, Percent_Identity=24.220623501199, Blast_Score=86, Evalue=8e-17,
Organism=Drosophila melanogaster, GI21356731, Length=162, Percent_Identity=30.8641975308642, Blast_Score=69, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATA_HELHP (Q7VIA7)

Other databases:

- EMBL:   AE017125
- RefSeq:   NP_860232.1
- ProteinModelPortal:   Q7VIA7
- SMR:   Q7VIA7
- GeneID:   1493755
- GenomeReviews:   AE017125_GR
- KEGG:   hhe:HH0701
- NMPDR:   fig|235279.1.peg.701
- HOGENOM:   HBG481888
- OMA:   TESSCYG
- ProtClustDB:   PRK00012
- BioCyc:   HHEP235279:HH_0701-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00120
- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412
- Gene3D:   G3DSA:3.90.1300.10
- PANTHER:   PTHR11895
- TIGRFAMs:   TIGR00132

Pfam domain/function: PF01425 Amidase; SSF75304 Amidase_sig_enz

EC number: 3.5.1.4

Molecular weight: Translated: 48776; Mature: 48776

Theoretical pI: Translated: 7.91; Mature: 7.91

Prosite motif: PS00571 AMIDASES

Important sites: ACT_SITE 51-51 ACT_SITE 126-126 ACT_SITE 150-150

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITLKEALNKSENELKEIKTLLRQRVKENIELNAYIGEINESGSGVPILIKDNINVKGWE
CEECHHHHCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCEEEEECCCCCCEEE
ITCASKILKGYVSPYNASVITKLHENKMCAFGRANMDEFAMGSTTESSCYGRVKNPLDTS
EHHHHHHHHHHCCCCCCHHHHHHHCCCEEECCCCCHHHHCCCCCCCHHHHHCCCCCCCCC
RVPGGSSGGSAAAVAAGLAIASLGSDTGGSIRQPAGFCGCVGLKPSYGRVSRYGLIAYSS
CCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCCHHHEEEEEECC
SLDQIGPITQNVEDAALLLDAISGYDKQDSTSANLPSLNTFKDLDPNARYKVAILKDFLK
CCHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHH
DATPQIQEAYNHTIKILESMGHTIVEKSMLDTRYHISAYYIICTAEASSNLARFDGIRYG
CCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHCEEEEEEEEEEEECCCCCCHHHHCCCCCC
TRSESKNLKDVYLNTRSAYFGEEVKRRILLGSFVLSSGYYDAYYLKAQQVRKKICADYES
CCCCCCCCHHHEEECHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
IFNECDSILLPIAPSVAPKFGSTHSSLEMYLSDIYTIGVNLAGLPALCMPVSKDKNGLSI
HHHHHHHEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHEEECCCCCCCHHH
GMQFIGAKFKEQNILNIAYGLEKEINN
HHHHHCCHHCCCCEEEHHHHHHHHCCC
>Mature Secondary Structure
MITLKEALNKSENELKEIKTLLRQRVKENIELNAYIGEINESGSGVPILIKDNINVKGWE
CEECHHHHCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCEEEEECCCCCCEEE
ITCASKILKGYVSPYNASVITKLHENKMCAFGRANMDEFAMGSTTESSCYGRVKNPLDTS
EHHHHHHHHHHCCCCCCHHHHHHHCCCEEECCCCCHHHHCCCCCCCHHHHHCCCCCCCCC
RVPGGSSGGSAAAVAAGLAIASLGSDTGGSIRQPAGFCGCVGLKPSYGRVSRYGLIAYSS
CCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCCHHHEEEEEECC
SLDQIGPITQNVEDAALLLDAISGYDKQDSTSANLPSLNTFKDLDPNARYKVAILKDFLK
CCHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHH
DATPQIQEAYNHTIKILESMGHTIVEKSMLDTRYHISAYYIICTAEASSNLARFDGIRYG
CCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHCEEEEEEEEEEEECCCCCCHHHHCCCCCC
TRSESKNLKDVYLNTRSAYFGEEVKRRILLGSFVLSSGYYDAYYLKAQQVRKKICADYES
CCCCCCCCHHHEEECHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
IFNECDSILLPIAPSVAPKFGSTHSSLEMYLSDIYTIGVNLAGLPALCMPVSKDKNGLSI
HHHHHHHEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHEEECCCCCCCHHH
GMQFIGAKFKEQNILNIAYGLEKEINN
HHHHHCCHHCCCCEEEHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: monocarboxylic acid amide; H2O

Specific reaction: A monocarboxylic acid amide + H2O = a monocarboxylate + NH3

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12810954