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The map label for this gene is lysC

Identifier: 32266117

GI number: 32266117

Start: 596478

End: 597683

Strand: Direct

Name: lysC

Synonym: HH0618

Alternate gene names: 32266117

Gene position: 596478-597683 (Clockwise)

Preceding gene: 32266116

Following gene: 32266118

Centisome position: 33.15

GC content: 38.14

Gene sequence:

>1206_bases
ATGCTAATCGTGCAAAAATATGGTGGCACGAGTATGGGAGATTGTGATAGGATCCATAATGTCGCCAAACGCGTTATTAA
AAGTAAAGCCCAAGGACATAAGCTTGTGGTTGTCGTATCTGCAATGGGAGGAGAAACAGATAGACTTATTAACTTTACAC
AACACTTCACGCCATTGCCAAATACACGCGAAGTTGATATGGCACTAAGTGCTGGAGAAAGGGTAAGCGCTGCACTCCTT
GCTATTGCTTTGGAATCAATGGGCGTAAAGGCAATTTCGCTAAGCGGTAGAGGAGCTGGAATTATCACTGATGAATTTCA
TACTAAAGCACGTATTTCTTATATTGATACAACCAAAATTTTTCATCTTCTTAATGAAGATTATGTAGTCGTAGTTGCAG
GGTTTCAAGGCATATCACAACAAGGTGAAGTAACTACGCTTGGGCGAGGAGGGAGTGATTTATCTGCTGTAGCATTAGCT
GGCGCACTTAATGCTACTGTATGTGAGATTTATAGCGATGTTGATGGTATTTATACAACAGATCCGCGCATTGTTAAAAA
CGCACGCAAATTAGAAAAAATTAGTTATGATGAAATGTTGGAGCTTGCCTCTATGGGTGCGAAAGTTTTGCTTAATCGCT
CTGTGGAACTCGCTAAAAAACTCAATGTCAATCTTATCTGCGCTAGCTCTTTTAGCGAAGCTCAAGGCACACTTATTACC
AAAGAGGAGGATATAATGGAAAAACCAGTCGTCAGTGGCGTAGCACTTGATAAAAATCAAGCACGTGTAAGTTTAGCTGA
TGTGCAAGACCGCCCAGGTATTGCTGCAGATATTTTTGGACTACTTGCAGATAATAATATTAATGTAGATATGATAGTTC
AAACAATTGGGCGTGATGGCAAAACTGATATTGATTTTACTATTCCTAAAACTGAAGTTACCCTTACACAACAAGTATTA
GAAAAATTTAAAGATGATTTGGGCAATATCGAATATGATAACAACATTGCTAAAGTCTCTATTGTAGGAGTAGGTATGAA
ATCACATTCTGGCGTAGCAAGCACAGCATTTAAAGCGTTAGCACAAGATGATATTAATATTATGATGATAAGCACAAGTG
AAATTAAAATTTCAATGATTATTAACGCCCAATTTGCTGAGCTTGCCGTGTGCAAGCTTCATAGTGTTTATGAACTTGAT
AAATAA

Upstream 100 bases:

>100_bases
CAAGTTTTAGAGTATTTTAAAAAAGAAGGTTATCTCTAGTGATATATTTTAAGGAAGAACATTTTATGATTACGCCTTTA
TTTTACACTCAAGGATATAT

Downstream 100 bases:

>100_bases
ATGCGGATTTACTATGGAAAATATCAGTGATTGGTTTTTGCGTATCATACGTGAAGATGAAAAAAAAGGTATTATGAGTG
GGTGGATTGAAGAAAAACGT

Product: aspartate kinase

Products: NA

Alternate protein names: Aspartate kinase [H]

Number of amino acids: Translated: 401; Mature: 401

Protein sequence:

>401_residues
MLIVQKYGGTSMGDCDRIHNVAKRVIKSKAQGHKLVVVVSAMGGETDRLINFTQHFTPLPNTREVDMALSAGERVSAALL
AIALESMGVKAISLSGRGAGIITDEFHTKARISYIDTTKIFHLLNEDYVVVVAGFQGISQQGEVTTLGRGGSDLSAVALA
GALNATVCEIYSDVDGIYTTDPRIVKNARKLEKISYDEMLELASMGAKVLLNRSVELAKKLNVNLICASSFSEAQGTLIT
KEEDIMEKPVVSGVALDKNQARVSLADVQDRPGIAADIFGLLADNNINVDMIVQTIGRDGKTDIDFTIPKTEVTLTQQVL
EKFKDDLGNIEYDNNIAKVSIVGVGMKSHSGVASTAFKALAQDDINIMMISTSEIKISMIINAQFAELAVCKLHSVYELD
K

Sequences:

>Translated_401_residues
MLIVQKYGGTSMGDCDRIHNVAKRVIKSKAQGHKLVVVVSAMGGETDRLINFTQHFTPLPNTREVDMALSAGERVSAALL
AIALESMGVKAISLSGRGAGIITDEFHTKARISYIDTTKIFHLLNEDYVVVVAGFQGISQQGEVTTLGRGGSDLSAVALA
GALNATVCEIYSDVDGIYTTDPRIVKNARKLEKISYDEMLELASMGAKVLLNRSVELAKKLNVNLICASSFSEAQGTLIT
KEEDIMEKPVVSGVALDKNQARVSLADVQDRPGIAADIFGLLADNNINVDMIVQTIGRDGKTDIDFTIPKTEVTLTQQVL
EKFKDDLGNIEYDNNIAKVSIVGVGMKSHSGVASTAFKALAQDDINIMMISTSEIKISMIINAQFAELAVCKLHSVYELD
K
>Mature_401_residues
MLIVQKYGGTSMGDCDRIHNVAKRVIKSKAQGHKLVVVVSAMGGETDRLINFTQHFTPLPNTREVDMALSAGERVSAALL
AIALESMGVKAISLSGRGAGIITDEFHTKARISYIDTTKIFHLLNEDYVVVVAGFQGISQQGEVTTLGRGGSDLSAVALA
GALNATVCEIYSDVDGIYTTDPRIVKNARKLEKISYDEMLELASMGAKVLLNRSVELAKKLNVNLICASSFSEAQGTLIT
KEEDIMEKPVVSGVALDKNQARVSLADVQDRPGIAADIFGLLADNNINVDMIVQTIGRDGKTDIDFTIPKTEVTLTQQVL
EKFKDDLGNIEYDNNIAKVSIVGVGMKSHSGVASTAFKALAQDDINIMMISTSEIKISMIINAQFAELAVCKLHSVYELD
K

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ACT domains [H]

Homologues:

Organism=Escherichia coli, GI1786183, Length=267, Percent_Identity=36.3295880149813, Blast_Score=149, Evalue=2e-37,
Organism=Escherichia coli, GI1790455, Length=454, Percent_Identity=27.5330396475771, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1790376, Length=174, Percent_Identity=32.7586206896552, Blast_Score=77, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6320893, Length=370, Percent_Identity=28.6486486486486, Blast_Score=106, Evalue=8e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR001048
- InterPro:   IPR005260
- InterPro:   IPR001341
- InterPro:   IPR018042 [H]

Pfam domain/function: PF00696 AA_kinase; PF01842 ACT [H]

EC number: =2.7.2.4 [H]

Molecular weight: Translated: 43228; Mature: 43228

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: PS00324 ASPARTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIVQKYGGTSMGDCDRIHNVAKRVIKSKAQGHKLVVVVSAMGGETDRLINFTQHFTPLP
CEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHEEHHHHCCCCC
NTREVDMALSAGERVSAALLAIALESMGVKAISLSGRGAGIITDEFHTKARISYIDTTKI
CCCEEEEECCCCCHHHHHHHHHHHHHCCEEEEEECCCCCEEEECCCCCCEEEEEEHHHHH
FHLLNEDYVVVVAGFQGISQQGEVTTLGRGGSDLSAVALAGALNATVCEIYSDVDGIYTT
HHHHCCCEEEEEECCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEC
DPRIVKNARKLEKISYDEMLELASMGAKVLLNRSVELAKKLNVNLICASSFSEAQGTLIT
CCHHHHHHHHHHHCCHHHHHHHHHCCHHEECCCCHHHHHHCCEEEEEECCCCCCCCEEEE
KEEDIMEKPVVSGVALDKNQARVSLADVQDRPGIAADIFGLLADNNINVDMIVQTIGRDG
CHHHHHHCCCCCCEEECCCCCEEEEECCCCCCCHHHHHHHHHCCCCCCCEEHEEECCCCC
KTDIDFTIPKTEVTLTQQVLEKFKDDLGNIEYDNNIAKVSIVGVGMKSHSGVASTAFKAL
CCCEEEEECCCHHHHHHHHHHHHHHHHCCCEECCCEEEEEEEEECCCCCCCHHHHHHHHH
AQDDINIMMISTSEIKISMIINAQFAELAVCKLHSVYELDK
HCCCCEEEEEEECCEEEEEEEECCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLIVQKYGGTSMGDCDRIHNVAKRVIKSKAQGHKLVVVVSAMGGETDRLINFTQHFTPLP
CEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHEEHHHHCCCCC
NTREVDMALSAGERVSAALLAIALESMGVKAISLSGRGAGIITDEFHTKARISYIDTTKI
CCCEEEEECCCCCHHHHHHHHHHHHHCCEEEEEECCCCCEEEECCCCCCEEEEEEHHHHH
FHLLNEDYVVVVAGFQGISQQGEVTTLGRGGSDLSAVALAGALNATVCEIYSDVDGIYTT
HHHHCCCEEEEEECCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEC
DPRIVKNARKLEKISYDEMLELASMGAKVLLNRSVELAKKLNVNLICASSFSEAQGTLIT
CCHHHHHHHHHHHCCHHHHHHHHHCCHHEECCCCHHHHHHCCEEEEEECCCCCCCCEEEE
KEEDIMEKPVVSGVALDKNQARVSLADVQDRPGIAADIFGLLADNNINVDMIVQTIGRDG
CHHHHHHCCCCCCEEECCCCCEEEEECCCCCCCHHHHHHHHHCCCCCCCEEHEEECCCCC
KTDIDFTIPKTEVTLTQQVLEKFKDDLGNIEYDNNIAKVSIVGVGMKSHSGVASTAFKAL
CCCEEEEECCCHHHHHHHHHHHHHHHHCCCEECCCEEEEEEEEECCCCCCCHHHHHHHHH
AQDDINIMMISTSEIKISMIINAQFAELAVCKLHSVYELDK
HCCCCEEEEEEECCEEEEEEEECCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]