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The map label for this gene is pyrB

Identifier: 32266020

GI number: 32266020

Start: 502410

End: 503303

Strand: Direct

Name: pyrB

Synonym: HH0521

Alternate gene names: 32266020

Gene position: 502410-503303 (Clockwise)

Preceding gene: 32266016

Following gene: 32266021

Centisome position: 27.92

GC content: 39.6

Gene sequence:

>894_bases
ATGCGCCCGCCCAAACATCTCTTACGCACAAGCGATCTTGATAATTCACAAATAGAAACAATTTTACAACAAGCCCAAAC
CTACAAAGATATGCACCATAGAGAGAATCTTAAAAACAAAACTATTATTACCATTTTTTTTGAAAACTCCACGCGCACAC
TCTCAAGCTTTGAAATCGCTGCTAAACGCTTAAGTGCTGATGTTGTGCGACTTGATGTGAGTAAAAGCTCCACTACAAAG
GGAGAGAGTATGTCTGATACTGCAGCCAATCTCAATGCAATGAATCCAAGCGCAATTATCATTCGTCATAAAAATGCAGG
AGCTGGATATTATCTTAAATTCCAAGTGAGCTGCCCTATCATCAATGCAGGAGATGGTGCGCACGCACACCCTACACAAG
CCCTACTTGATTTACTCACACTTAAAGAACATTTTGATAATGATTTAAACAATCTCAAAGGTAAAAAAATTGCAATTATT
GGTGATATTGTAAATTCCCGTGTGGCAAATAGCAATATTGAGCTGCTCTCGCGTTTTGGTATGGAAGTGATTCTCGTTGC
CCCACCTCATTTTCTTCCATCCACACATTTACGCACTTGCTACTCTTTGCGAGAAATTGCCAAAGAAGTTGATGTATTTA
TGAGTTTGCGCACTCAAACAGAAAGACACGATAAACAAATTTATGGCTCACTCAAAGACTATGCTTCACAATACTGCCTC
ACGCCCGAGATTCTAAGCGATAGAGATGTTATTGTTTTACATCCTGGACCTGTTCATCGTAACATTGATATTGATGATGA
AGTGCTTAAAGACCCACGTTGCAAAGTTTTAGAGCAGGTAACTAACGGCGTATGTGTGCGTATGGCAGTGCTTGAATTTT
GTATCTGCACATAA

Upstream 100 bases:

>100_bases
ATCTTTAATTTTGTAAATGATACTTATTAGATTCTAAAATTATGGGAATTTTATCACAAAGTTTGCTACAATACCCAAAT
TTATCCCCAAAAGGAGATTT

Downstream 100 bases:

>100_bases
AGTTTATTTGATATTAAGTCTCAATTAAGCATAAAATGTTAGAATACATATTGTATTCTAAAAAATACGTAAATTTATTT
GAAATATCTGCAAATATTTC

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MRPPKHLLRTSDLDNSQIETILQQAQTYKDMHHRENLKNKTIITIFFENSTRTLSSFEIAAKRLSADVVRLDVSKSSTTK
GESMSDTAANLNAMNPSAIIIRHKNAGAGYYLKFQVSCPIINAGDGAHAHPTQALLDLLTLKEHFDNDLNNLKGKKIAII
GDIVNSRVANSNIELLSRFGMEVILVAPPHFLPSTHLRTCYSLREIAKEVDVFMSLRTQTERHDKQIYGSLKDYASQYCL
TPEILSDRDVIVLHPGPVHRNIDIDDEVLKDPRCKVLEQVTNGVCVRMAVLEFCICT

Sequences:

>Translated_297_residues
MRPPKHLLRTSDLDNSQIETILQQAQTYKDMHHRENLKNKTIITIFFENSTRTLSSFEIAAKRLSADVVRLDVSKSSTTK
GESMSDTAANLNAMNPSAIIIRHKNAGAGYYLKFQVSCPIINAGDGAHAHPTQALLDLLTLKEHFDNDLNNLKGKKIAII
GDIVNSRVANSNIELLSRFGMEVILVAPPHFLPSTHLRTCYSLREIAKEVDVFMSLRTQTERHDKQIYGSLKDYASQYCL
TPEILSDRDVIVLHPGPVHRNIDIDDEVLKDPRCKVLEQVTNGVCVRMAVLEFCICT
>Mature_297_residues
MRPPKHLLRTSDLDNSQIETILQQAQTYKDMHHRENLKNKTIITIFFENSTRTLSSFEIAAKRLSADVVRLDVSKSSTTK
GESMSDTAANLNAMNPSAIIIRHKNAGAGYYLKFQVSCPIINAGDGAHAHPTQALLDLLTLKEHFDNDLNNLKGKKIAII
GDIVNSRVANSNIELLSRFGMEVILVAPPHFLPSTHLRTCYSLREIAKEVDVFMSLRTQTERHDKQIYGSLKDYASQYCL
TPEILSDRDVIVLHPGPVHRNIDIDDEVLKDPRCKVLEQVTNGVCVRMAVLEFCICT

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI18105007, Length=307, Percent_Identity=32.5732899022801, Blast_Score=151, Evalue=7e-37,
Organism=Escherichia coli, GI2367364, Length=308, Percent_Identity=32.7922077922078, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI1790703, Length=322, Percent_Identity=27.9503105590062, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1786469, Length=323, Percent_Identity=27.5541795665635, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI48994908, Length=215, Percent_Identity=31.6279069767442, Blast_Score=69, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI193204318, Length=336, Percent_Identity=30.6547619047619, Blast_Score=108, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6322331, Length=316, Percent_Identity=31.6455696202532, Blast_Score=129, Evalue=6e-31,
Organism=Drosophila melanogaster, GI24642586, Length=312, Percent_Identity=31.4102564102564, Blast_Score=132, Evalue=3e-31,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): PYRB_HELHP (Q7VIT3)

Other databases:

- EMBL:   AE017125
- RefSeq:   NP_860052.1
- ProteinModelPortal:   Q7VIT3
- SMR:   Q7VIT3
- GeneID:   1493575
- GenomeReviews:   AE017125_GR
- KEGG:   hhe:HH0521
- NMPDR:   fig|235279.1.peg.521
- HOGENOM:   HBG579429
- OMA:   MNRGLEI
- ProtClustDB:   PRK00856
- BioCyc:   HHEP235279:HH_0521-MONOMER
- BRENDA:   2.1.3.2
- HAMAP:   MF_00001
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082
- PRINTS:   PR00100
- PRINTS:   PR00101
- TIGRFAMs:   TIGR00670

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.2

Molecular weight: Translated: 33352; Mature: 33352

Theoretical pI: Translated: 7.60; Mature: 7.60

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPPKHLLRTSDLDNSQIETILQQAQTYKDMHHRENLKNKTIITIFFENSTRTLSSFEIA
CCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHH
AKRLSADVVRLDVSKSSTTKGESMSDTAANLNAMNPSAIIIRHKNAGAGYYLKFQVSCPI
HHHHCCCEEEEECCCCCCCCCCCCCHHHHHCCCCCCCEEEEEECCCCCCEEEEEEEECCE
INAGDGAHAHPTQALLDLLTLKEHFDNDLNNLKGKKIAIIGDIVNSRVANSNIELLSRFG
EECCCCCCCCHHHHHHHHHHHHHHHCCHHHHCCCCEEEEEEHHHHHHHCCCCHHHHHHCC
MEVILVAPPHFLPSTHLRTCYSLREIAKEVDVFMSLRTQTERHDKQIYGSLKDYASQYCL
CEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TPEILSDRDVIVLHPGPVHRNIDIDDEVLKDPRCKVLEQVTNGVCVRMAVLEFCICT
CHHHHCCCCEEEECCCCCCCCCCCCHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MRPPKHLLRTSDLDNSQIETILQQAQTYKDMHHRENLKNKTIITIFFENSTRTLSSFEIA
CCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHH
AKRLSADVVRLDVSKSSTTKGESMSDTAANLNAMNPSAIIIRHKNAGAGYYLKFQVSCPI
HHHHCCCEEEEECCCCCCCCCCCCCHHHHHCCCCCCCEEEEEECCCCCCEEEEEEEECCE
INAGDGAHAHPTQALLDLLTLKEHFDNDLNNLKGKKIAIIGDIVNSRVANSNIELLSRFG
EECCCCCCCCHHHHHHHHHHHHHHHCCHHHHCCCCEEEEEEHHHHHHHCCCCHHHHHHCC
MEVILVAPPHFLPSTHLRTCYSLREIAKEVDVFMSLRTQTERHDKQIYGSLKDYASQYCL
CEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TPEILSDRDVIVLHPGPVHRNIDIDDEVLKDPRCKVLEQVTNGVCVRMAVLEFCICT
CHHHHCCCCEEEECCCCCCCCCCCCHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12810954