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The map label for this gene is cysS

Identifier: 32266016

GI number: 32266016

Start: 498696

End: 500162

Strand: Direct

Name: cysS

Synonym: HH0517

Alternate gene names: 32266016

Gene position: 498696-500162 (Clockwise)

Preceding gene: 32266015

Following gene: 32266020

Centisome position: 27.72

GC content: 35.58

Gene sequence:

>1467_bases
ATGATTACTCTTTTTGATAGTGCTAAAAAACAAAAGATCCCTTTTACTCCTATACAAGATAATCAGATTCGATTATATGT
GTGCGGTCCAACTGTGTATGATGATGCTCATTTAGGGCACGCTCGTAGCTCTATTGTTTTTGATTTATGGCGACGATTAT
TTTTATTTTTGGGATTTGAAGTAGTATTTGTTAAAAACTTTACAGACATTGATGATAAAATTATTAAAAAATCTCTTCAA
AACAATGCAAGTGTGCAAGAGATAGGTTCGCACTATATCCATTCATATCTTAAAGATATGGCTTCTCTTGGCGTATTGCG
TGCAGATATTGAGCCAAAAGCAACCGATAATCTACCCCAAATGTGCGAGATGATACAAACCTTGCTTCATAAAGGTTACG
CTTATGAGGGTGAAAATAGTGATATTTATCTTCGTATCCACAAAGATAAAAACTATGGTGCTCTCTCTCAACGCCTTGAA
CAAAGCCATACCCAAAGTCGTATTCAAAATGCACAAGACAAGCTTGAACATAATGATTTTGCCCTTTGGAAAGGTTATAA
GGGAGAGAATGATATTGCATATGATAGTCCATTTGGCAAAGGGCGTCCCGGCTGGCATATTGAATGTTCTGCAATGATTG
AAAAACACTTAGCATACCAAAATGAAGAATATGGTATTGATATTCACGCAGGAGGATCAGATTTGCTCTTCCCTCATCAC
GAAAATGAAGCCTCACAAACGCGTTGTGCTACAGGAAGAGAAATTGCTAAATATTGGCTACACAATGGTTTTGTGAATAT
CAATGGTGAAAAAATGAGCAAATCTCTTGGCAATAGCTTTTTTATCAAAGATGCGCTAAAAGTCTATGATGGAGAGATTT
TACGTAATTATTTGCTGGGCGTGCATTATCGCTTGGCACTCAATTTTAATGAAGAGGATTTGTTACAAAGCAAAAAACGA
CTTGATAAACTCTATCGCCTTAAAAAACGTGTTATAGAATCTCACATACAATCTCCTCAAATTACAAGAGAAGAAATAAA
GCAATTTACTCACAATATCAAAGAAACCGCCAAAGAAGCGCATAAAACATTTTTATCCTCACTTGTTGAGGCTTTAAGCG
ATGATTATAATATCTCAAAAGCTTTGAGCATTATTGAGGATATGCTCTCTACAAGCAATGAATATTTAGACAAAAATCCT
AAAGATAAAGCCTATAAGCAAGCTATTAAAGCAAATCTCGCGTGTATTGAATTTTTACTCGGGCTTGGAGGTAAATCCAC
ACAAAGTTATTTTCAGCTTGGACTTGATGAAAAAACTAAGCAAGAGATAGAATCTAAAATTGCTAAGCGTCAAGAAGCAA
AAGCACAAAAAAACTATGCACTTGCTGATAAGTTACGCGATGAGCTAAAATCGCAAGGTATTGAAATTATGGACACTCCT
CAAGGCAGCACTTGGGAAAAAATCTAA

Upstream 100 bases:

>100_bases
TTGTAGGCATTGTGGGTTTGGAATCTCTACTTTTGTGGTATCTCAAACCCTATGTCAAAGCACTCGTTGATGCTTTTCAT
CTCTTTGTAAGGAACATTTT

Downstream 100 bases:

>100_bases
GCTACATATCTACATATATCATCTGTGCAGCACTCAAAATTACTTTAGATGTAATAATATCTTGGGTAATGCGCTCTTTA
AGCAAATTTAAATCACTAAA

Product: cysteinyl-tRNA synthetase

Products: NA

Alternate protein names: Cysteine--tRNA ligase; CysRS

Number of amino acids: Translated: 488; Mature: 488

Protein sequence:

>488_residues
MITLFDSAKKQKIPFTPIQDNQIRLYVCGPTVYDDAHLGHARSSIVFDLWRRLFLFLGFEVVFVKNFTDIDDKIIKKSLQ
NNASVQEIGSHYIHSYLKDMASLGVLRADIEPKATDNLPQMCEMIQTLLHKGYAYEGENSDIYLRIHKDKNYGALSQRLE
QSHTQSRIQNAQDKLEHNDFALWKGYKGENDIAYDSPFGKGRPGWHIECSAMIEKHLAYQNEEYGIDIHAGGSDLLFPHH
ENEASQTRCATGREIAKYWLHNGFVNINGEKMSKSLGNSFFIKDALKVYDGEILRNYLLGVHYRLALNFNEEDLLQSKKR
LDKLYRLKKRVIESHIQSPQITREEIKQFTHNIKETAKEAHKTFLSSLVEALSDDYNISKALSIIEDMLSTSNEYLDKNP
KDKAYKQAIKANLACIEFLLGLGGKSTQSYFQLGLDEKTKQEIESKIAKRQEAKAQKNYALADKLRDELKSQGIEIMDTP
QGSTWEKI

Sequences:

>Translated_488_residues
MITLFDSAKKQKIPFTPIQDNQIRLYVCGPTVYDDAHLGHARSSIVFDLWRRLFLFLGFEVVFVKNFTDIDDKIIKKSLQ
NNASVQEIGSHYIHSYLKDMASLGVLRADIEPKATDNLPQMCEMIQTLLHKGYAYEGENSDIYLRIHKDKNYGALSQRLE
QSHTQSRIQNAQDKLEHNDFALWKGYKGENDIAYDSPFGKGRPGWHIECSAMIEKHLAYQNEEYGIDIHAGGSDLLFPHH
ENEASQTRCATGREIAKYWLHNGFVNINGEKMSKSLGNSFFIKDALKVYDGEILRNYLLGVHYRLALNFNEEDLLQSKKR
LDKLYRLKKRVIESHIQSPQITREEIKQFTHNIKETAKEAHKTFLSSLVEALSDDYNISKALSIIEDMLSTSNEYLDKNP
KDKAYKQAIKANLACIEFLLGLGGKSTQSYFQLGLDEKTKQEIESKIAKRQEAKAQKNYALADKLRDELKSQGIEIMDTP
QGSTWEKI
>Mature_488_residues
MITLFDSAKKQKIPFTPIQDNQIRLYVCGPTVYDDAHLGHARSSIVFDLWRRLFLFLGFEVVFVKNFTDIDDKIIKKSLQ
NNASVQEIGSHYIHSYLKDMASLGVLRADIEPKATDNLPQMCEMIQTLLHKGYAYEGENSDIYLRIHKDKNYGALSQRLE
QSHTQSRIQNAQDKLEHNDFALWKGYKGENDIAYDSPFGKGRPGWHIECSAMIEKHLAYQNEEYGIDIHAGGSDLLFPHH
ENEASQTRCATGREIAKYWLHNGFVNINGEKMSKSLGNSFFIKDALKVYDGEILRNYLLGVHYRLALNFNEEDLLQSKKR
LDKLYRLKKRVIESHIQSPQITREEIKQFTHNIKETAKEAHKTFLSSLVEALSDDYNISKALSIIEDMLSTSNEYLDKNP
KDKAYKQAIKANLACIEFLLGLGGKSTQSYFQLGLDEKTKQEIESKIAKRQEAKAQKNYALADKLRDELKSQGIEIMDTP
QGSTWEKI

Specific function: Unknown

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI13375695, Length=529, Percent_Identity=30.0567107750473, Blast_Score=211, Evalue=1e-54,
Organism=Homo sapiens, GI21269877, Length=372, Percent_Identity=29.8387096774194, Blast_Score=140, Evalue=3e-33,
Organism=Homo sapiens, GI303304987, Length=372, Percent_Identity=29.8387096774194, Blast_Score=140, Evalue=4e-33,
Organism=Homo sapiens, GI62240992, Length=372, Percent_Identity=29.8387096774194, Blast_Score=140, Evalue=4e-33,
Organism=Homo sapiens, GI10835051, Length=372, Percent_Identity=29.8387096774194, Blast_Score=139, Evalue=4e-33,
Organism=Escherichia coli, GI1786737, Length=490, Percent_Identity=38.5714285714286, Blast_Score=343, Evalue=1e-95,
Organism=Caenorhabditis elegans, GI17509795, Length=384, Percent_Identity=28.3854166666667, Blast_Score=136, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI17509797, Length=384, Percent_Identity=28.3854166666667, Blast_Score=136, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6324082, Length=359, Percent_Identity=28.9693593314763, Blast_Score=147, Evalue=5e-36,
Organism=Drosophila melanogaster, GI19922218, Length=520, Percent_Identity=30.1923076923077, Blast_Score=236, Evalue=2e-62,
Organism=Drosophila melanogaster, GI19922364, Length=357, Percent_Identity=31.9327731092437, Blast_Score=154, Evalue=2e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYC_HELHP (Q7VIT7)

Other databases:

- EMBL:   AE017125
- RefSeq:   NP_860048.1
- ProteinModelPortal:   Q7VIT7
- SMR:   Q7VIT7
- GeneID:   1493571
- GenomeReviews:   AE017125_GR
- KEGG:   hhe:HH0517
- NMPDR:   fig|235279.1.peg.517
- HOGENOM:   HBG327651
- OMA:   DFDALNM
- ProtClustDB:   PRK00260
- BioCyc:   HHEP235279:HH_0517-MONOMER
- BRENDA:   6.1.1.16
- GO:   GO:0005737
- HAMAP:   MF_00041_B
- InterPro:   IPR015803
- InterPro:   IPR015273
- InterPro:   IPR014729
- InterPro:   IPR009080
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10890
- PRINTS:   PR00983
- SMART:   SM00840
- TIGRFAMs:   TIGR00435

Pfam domain/function: PF01406 tRNA-synt_1e; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.16

Molecular weight: Translated: 56028; Mature: 56028

Theoretical pI: Translated: 7.32; Mature: 7.32

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 274-274

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITLFDSAKKQKIPFTPIQDNQIRLYVCGPTVYDDAHLGHARSSIVFDLWRRLFLFLGFE
CEEECCCCCCCCCCCCCCCCCCEEEEEECCCEECCCCCCHHHHHHHHHHHHHHHHHHCCH
VVFVKNFTDIDDKIIKKSLQNNASVQEIGSHYIHSYLKDMASLGVLRADIEPKATDNLPQ
HEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCHHHCCCCCCCCCCHHH
MCEMIQTLLHKGYAYEGENSDIYLRIHKDKNYGALSQRLEQSHTQSRIQNAQDKLEHNDF
HHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCE
ALWKGYKGENDIAYDSPFGKGRPGWHIECSAMIEKHLAYQNEEYGIDIHAGGSDLLFPHH
EEECCCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCC
ENEASQTRCATGREIAKYWLHNGFVNINGEKMSKSLGNSFFIKDALKVYDGEILRNYLLG
CCCHHHHHHHHHHHHHHHHHHCCEEEECHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
VHYRLALNFNEEDLLQSKKRLDKLYRLKKRVIESHIQSPQITREEIKQFTHNIKETAKEA
EEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
HKTFLSSLVEALSDDYNISKALSIIEDMLSTSNEYLDKNPKDKAYKQAIKANLACIEFLL
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHCCCCCHHHHHHHHHHHHHHHHHHH
GLGGKSTQSYFQLGLDEKTKQEIESKIAKRQEAKAQKNYALADKLRDELKSQGIEIMDTP
HCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECC
QGSTWEKI
CCCCCCCC
>Mature Secondary Structure
MITLFDSAKKQKIPFTPIQDNQIRLYVCGPTVYDDAHLGHARSSIVFDLWRRLFLFLGFE
CEEECCCCCCCCCCCCCCCCCCEEEEEECCCEECCCCCCHHHHHHHHHHHHHHHHHHCCH
VVFVKNFTDIDDKIIKKSLQNNASVQEIGSHYIHSYLKDMASLGVLRADIEPKATDNLPQ
HEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCHHHCCCCCCCCCCHHH
MCEMIQTLLHKGYAYEGENSDIYLRIHKDKNYGALSQRLEQSHTQSRIQNAQDKLEHNDF
HHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCE
ALWKGYKGENDIAYDSPFGKGRPGWHIECSAMIEKHLAYQNEEYGIDIHAGGSDLLFPHH
EEECCCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCC
ENEASQTRCATGREIAKYWLHNGFVNINGEKMSKSLGNSFFIKDALKVYDGEILRNYLLG
CCCHHHHHHHHHHHHHHHHHHCCEEEECHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
VHYRLALNFNEEDLLQSKKRLDKLYRLKKRVIESHIQSPQITREEIKQFTHNIKETAKEA
EEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
HKTFLSSLVEALSDDYNISKALSIIEDMLSTSNEYLDKNPKDKAYKQAIKANLACIEFLL
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHCCCCCHHHHHHHHHHHHHHHHHHH
GLGGKSTQSYFQLGLDEKTKQEIESKIAKRQEAKAQKNYALADKLRDELKSQGIEIMDTP
HCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECC
QGSTWEKI
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12810954