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The map label for this gene is prpC

Identifier: 32265897

GI number: 32265897

Start: 385421

End: 386569

Strand: Direct

Name: prpC

Synonym: HH0398

Alternate gene names: 32265897

Gene position: 385421-386569 (Clockwise)

Preceding gene: 32265896

Following gene: 32265898

Centisome position: 21.42

GC content: 38.9

Gene sequence:

>1149_bases
ATGGGAGAAACAATCAAAAATACAAAAGTAGGCGGACTTGCAGGCATTACTGCAGGACAATCAGCTATCTGCACTGTTGG
CACAGGGCACGGACTTAATTATAGAGGATATGATATTTATGATTTGGCACGTGAATGTGAATTTGAAGAAGTAGCTTATT
TGCTTTTAAAAGAAAAACTACCAAATGCAAATGAACTAGCGGCATTTAAAAAAGAGCTTATAGCCGCAAGAGATTTGCCC
AAAGCACTTAAGGATACATTAAAACTCTTGCCAAAAAACGCTCACCCAATGGATATTATGCGCACAACTTGTTCTATGTT
AGGCTGTCTTGAGCCTGAAACTTATGACATTGTAAAAATGTCTTTTCCTATGCAGAATCAAATTGCCATAAGACTTTTAG
GGATTTTTCCTTCAGCCCTCACTTTTTGGCATCATTACCATCATAATGGCAAAGAAATCAGCACTCAATCTAACCAAGAT
TCTATTGCTGGACATTTTTTGGAACTTCTTCATCAAAAACCACCAAAAGAGCTATGGATAAAAGCAATGCACGCAAGTTT
GATTCTCTATGCAGAACACGAATTTAATGCAAGCACTTTTACCGCGCGCGTGATTACAGCAACTATGTCAGATGTGTATT
CAGGAGTCACGGGGGCAATTGGTGCGCTTAGAGGTCCTTTACACGGAGGGGCAAATGAAGCGGCAATGGAGCTTATCGCC
GAATATAAAACTCCACAGGAGGCAGAAAAAGGCATTTTAGATAAACTTGCAAGAAAAGATAAAATTATGGGCTTTGGGCA
TCGCGTGTATGTCAAAGCTGACCCGCGCAATGTAGTAATAAAAGAATGGAGCAAGAAATTAAGCGAAGATGTGGGTGATA
CAAAGGGACTTTATCCTATTAGTGAAGCAATAGAAAAGGTAATGTGGGAACAAAAAAAGCTTTTTCCTAATCTTGATTTT
TATAGCGCGTCTGCTTATCATTTTATGGGGATTCCAACACCATATTTCACACCGATTTTTATTTTTTCTCGCACAGCTGG
GTGGTTGGCACATATTTTTGAGCAAAGGGCAAATAATAAACTTATTCGTCCAAGTTCAGAATATATTGGACCAGATAAAA
AGGCATTTGTGCCATTGCATAATCGCTAG

Upstream 100 bases:

>100_bases
TCTATGCAAACTCGTGATGAACTTTATGAAATGCTCGGTTATCATACTTTTGAGAAGAAGCTTGATGAGCTTTTTAAACA
TAAAAAATAAGGAGAAACTT

Downstream 100 bases:

>100_bases
GCACATTTTTAATCTAAATTAAAGGAGAACACAATGAGTGTAGATATGGGTATTTTAGAGACACAACGACCGGAGTTTGA
TGAGCTATTAAGTAAAATTG

Product: citrate synthase PrpC

Products: NA

Alternate protein names: Citrate synthase 2; Methylcitrate synthase [H]

Number of amino acids: Translated: 382; Mature: 381

Protein sequence:

>382_residues
MGETIKNTKVGGLAGITAGQSAICTVGTGHGLNYRGYDIYDLARECEFEEVAYLLLKEKLPNANELAAFKKELIAARDLP
KALKDTLKLLPKNAHPMDIMRTTCSMLGCLEPETYDIVKMSFPMQNQIAIRLLGIFPSALTFWHHYHHNGKEISTQSNQD
SIAGHFLELLHQKPPKELWIKAMHASLILYAEHEFNASTFTARVITATMSDVYSGVTGAIGALRGPLHGGANEAAMELIA
EYKTPQEAEKGILDKLARKDKIMGFGHRVYVKADPRNVVIKEWSKKLSEDVGDTKGLYPISEAIEKVMWEQKKLFPNLDF
YSASAYHFMGIPTPYFTPIFIFSRTAGWLAHIFEQRANNKLIRPSSEYIGPDKKAFVPLHNR

Sequences:

>Translated_382_residues
MGETIKNTKVGGLAGITAGQSAICTVGTGHGLNYRGYDIYDLARECEFEEVAYLLLKEKLPNANELAAFKKELIAARDLP
KALKDTLKLLPKNAHPMDIMRTTCSMLGCLEPETYDIVKMSFPMQNQIAIRLLGIFPSALTFWHHYHHNGKEISTQSNQD
SIAGHFLELLHQKPPKELWIKAMHASLILYAEHEFNASTFTARVITATMSDVYSGVTGAIGALRGPLHGGANEAAMELIA
EYKTPQEAEKGILDKLARKDKIMGFGHRVYVKADPRNVVIKEWSKKLSEDVGDTKGLYPISEAIEKVMWEQKKLFPNLDF
YSASAYHFMGIPTPYFTPIFIFSRTAGWLAHIFEQRANNKLIRPSSEYIGPDKKAFVPLHNR
>Mature_381_residues
GETIKNTKVGGLAGITAGQSAICTVGTGHGLNYRGYDIYDLARECEFEEVAYLLLKEKLPNANELAAFKKELIAARDLPK
ALKDTLKLLPKNAHPMDIMRTTCSMLGCLEPETYDIVKMSFPMQNQIAIRLLGIFPSALTFWHHYHHNGKEISTQSNQDS
IAGHFLELLHQKPPKELWIKAMHASLILYAEHEFNASTFTARVITATMSDVYSGVTGAIGALRGPLHGGANEAAMELIAE
YKTPQEAEKGILDKLARKDKIMGFGHRVYVKADPRNVVIKEWSKKLSEDVGDTKGLYPISEAIEKVMWEQKKLFPNLDFY
SASAYHFMGIPTPYFTPIFIFSRTAGWLAHIFEQRANNKLIRPSSEYIGPDKKAFVPLHNR

Specific function: Catalyzes the synthesis of 2-methylcitrate from propionyl-CoA and oxaloacetate. Also catalyzes the condensation of oxaloacetate with acetyl-CoA but with a lower specificity [H]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Homo sapiens, GI38327625, Length=398, Percent_Identity=22.1105527638191, Blast_Score=80, Evalue=4e-15,
Organism=Escherichia coli, GI1786527, Length=380, Percent_Identity=53.1578947368421, Blast_Score=412, Evalue=1e-116,
Organism=Escherichia coli, GI1786939, Length=380, Percent_Identity=31.3157894736842, Blast_Score=184, Evalue=7e-48,
Organism=Caenorhabditis elegans, GI17555174, Length=387, Percent_Identity=24.5478036175711, Blast_Score=95, Evalue=7e-20,
Organism=Saccharomyces cerevisiae, GI6319850, Length=387, Percent_Identity=25.5813953488372, Blast_Score=97, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6324328, Length=401, Percent_Identity=25.4364089775561, Blast_Score=96, Evalue=9e-21,
Organism=Saccharomyces cerevisiae, GI6325257, Length=399, Percent_Identity=26.5664160401003, Blast_Score=83, Evalue=9e-17,
Organism=Drosophila melanogaster, GI21356863, Length=402, Percent_Identity=23.3830845771144, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24640126, Length=386, Percent_Identity=24.3523316062176, Blast_Score=88, Evalue=8e-18,
Organism=Drosophila melanogaster, GI24640124, Length=386, Percent_Identity=24.3523316062176, Blast_Score=88, Evalue=9e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011278
- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.5 [H]

Molecular weight: Translated: 42814; Mature: 42683

Theoretical pI: Translated: 8.54; Mature: 8.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGETIKNTKVGGLAGITAGQSAICTVGTGHGLNYRGYDIYDLARECEFEEVAYLLLKEKL
CCCCCCCCCCCCEEECCCCCCEEEEEECCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHC
PNANELAAFKKELIAARDLPKALKDTLKLLPKNAHPMDIMRTTCSMLGCLEPETYDIVKM
CCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEE
SFPMQNQIAIRLLGIFPSALTFWHHYHHNGKEISTQSNQDSIAGHFLELLHQKPPKELWI
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCHHHHHHHHHHHHCCCCHHHHH
KAMHASLILYAEHEFNASTFTARVITATMSDVYSGVTGAIGALRGPLHGGANEAAMELIA
HHHHHEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
EYKTPQEAEKGILDKLARKDKIMGFGHRVYVKADPRNVVIKEWSKKLSEDVGDTKGLYPI
HCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCHH
SEAIEKVMWEQKKLFPNLDFYSASAYHFMGIPTPYFTPIFIFSRTAGWLAHIFEQRANNK
HHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
LIRPSSEYIGPDKKAFVPLHNR
CCCCCHHHCCCCCCEECCCCCC
>Mature Secondary Structure 
GETIKNTKVGGLAGITAGQSAICTVGTGHGLNYRGYDIYDLARECEFEEVAYLLLKEKL
CCCCCCCCCCCEEECCCCCCEEEEEECCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHC
PNANELAAFKKELIAARDLPKALKDTLKLLPKNAHPMDIMRTTCSMLGCLEPETYDIVKM
CCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEE
SFPMQNQIAIRLLGIFPSALTFWHHYHHNGKEISTQSNQDSIAGHFLELLHQKPPKELWI
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCHHHHHHHHHHHHCCCCHHHHH
KAMHASLILYAEHEFNASTFTARVITATMSDVYSGVTGAIGALRGPLHGGANEAAMELIA
HHHHHEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
EYKTPQEAEKGILDKLARKDKIMGFGHRVYVKADPRNVVIKEWSKKLSEDVGDTKGLYPI
HCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCHH
SEAIEKVMWEQKKLFPNLDFYSASAYHFMGIPTPYFTPIFIFSRTAGWLAHIFEQRANNK
HHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
LIRPSSEYIGPDKKAFVPLHNR
CCCCCHHHCCCCCCEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 8508809; 9325432 [H]