Click here to switch to the map view.

The map label for this gene is prpB

Identifier: 32265896

GI number: 32265896

Start: 384535

End: 385410

Strand: Direct

Name: prpB

Synonym: HH0397

Alternate gene names: 32265896

Gene position: 384535-385410 (Clockwise)

Preceding gene: 32265895

Following gene: 32265897

Centisome position: 21.37

GC content: 39.38

Gene sequence:

>876_bases
ATGAAATCACAAGGAATAAAATTAAGAGAAGCCGTAGCGCAAAATAATCCTTTGCAAATTATCGGTGTAATTAACGCATA
TAGTGCTATACAAGCACAAAAAAGTGGAGCAAAAGCCCTCTATCTTAGTGGTGGTGCATTGGCTGCTATGAGTTTGGGAG
TGCCGGATTTGGGCATTAGCTCATTGGAAGATGTGTGTATTGATGTGCGTAGAATCACTGCAGCAAGCGATTTACCTTTA
CTTGTTGATGCAGATACAGGCTGGGGTGGGGCATTTAACATTGCACGCACTATCAAAGATTTAACACGTAGCGGTGCAGC
AGGTTGTCATATTGAAGACCAAGTGGCGCAAAAACGTTGCGGACACCGCCCTAATAAAGAACTTGTAAGCCTAGAGGAAA
TGTGCGATAGAATCAAAGCCGGAGTAGATGCAAAAATTGATGAAAACTTTGTATTGATGGCACGCACAGATGCACACGCA
AGTGAAGGACAGAATCGTGCGATTGAGAGAGCATTAGCTTATGTAGAGGCTGGAGCAGATATGATTTTTGCCGAAGCCAT
ACATACACTTGAAGAATATAAACAATTCACACAAAACATCAAAGTGCCTGTTTTAGCAAATATTACAGAATTTGGAAAAA
CCCCTTATTTTACGACCTCTGAACTTCAAAATGTTGGTGTAAAAATGGTGCTTTACCCACTTTCAGCAGCACGAGCGATG
AATAAAGCAGCACTTACAGTTTTCCAAGATATTATTAAAAATGGCTCACAAAAGCAAAGTATAGATTCTATGCAAACTCG
TGATGAACTTTATGAAATGCTCGGTTATCATACTTTTGAGAAGAAGCTTGATGAGCTTTTTAAACATAAAAAATAA

Upstream 100 bases:

>100_bases
ATGGAAGATGCCCTCAACTATTGAAGATTCAAGCGTTATGCCAGAGATAGAAAAAACACTTGCCACGCTTGGCTATCCAA
TCAAAACAAAGGAGTAAAAA

Downstream 100 bases:

>100_bases
GGAGAAACTTATGGGAGAAACAATCAAAAATACAAAAGTAGGCGGACTTGCAGGCATTACTGCAGGACAATCAGCTATCT
GCACTGTTGGCACAGGGCAC

Product: 2-methylisocitrate lyase

Products: NA

Alternate protein names: 2-methylisocitrate lyase [H]

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
MKSQGIKLREAVAQNNPLQIIGVINAYSAIQAQKSGAKALYLSGGALAAMSLGVPDLGISSLEDVCIDVRRITAASDLPL
LVDADTGWGGAFNIARTIKDLTRSGAAGCHIEDQVAQKRCGHRPNKELVSLEEMCDRIKAGVDAKIDENFVLMARTDAHA
SEGQNRAIERALAYVEAGADMIFAEAIHTLEEYKQFTQNIKVPVLANITEFGKTPYFTTSELQNVGVKMVLYPLSAARAM
NKAALTVFQDIIKNGSQKQSIDSMQTRDELYEMLGYHTFEKKLDELFKHKK

Sequences:

>Translated_291_residues
MKSQGIKLREAVAQNNPLQIIGVINAYSAIQAQKSGAKALYLSGGALAAMSLGVPDLGISSLEDVCIDVRRITAASDLPL
LVDADTGWGGAFNIARTIKDLTRSGAAGCHIEDQVAQKRCGHRPNKELVSLEEMCDRIKAGVDAKIDENFVLMARTDAHA
SEGQNRAIERALAYVEAGADMIFAEAIHTLEEYKQFTQNIKVPVLANITEFGKTPYFTTSELQNVGVKMVLYPLSAARAM
NKAALTVFQDIIKNGSQKQSIDSMQTRDELYEMLGYHTFEKKLDELFKHKK
>Mature_291_residues
MKSQGIKLREAVAQNNPLQIIGVINAYSAIQAQKSGAKALYLSGGALAAMSLGVPDLGISSLEDVCIDVRRITAASDLPL
LVDADTGWGGAFNIARTIKDLTRSGAAGCHIEDQVAQKRCGHRPNKELVSLEEMCDRIKAGVDAKIDENFVLMARTDAHA
SEGQNRAIERALAYVEAGADMIFAEAIHTLEEYKQFTQNIKVPVLANITEFGKTPYFTTSELQNVGVKMVLYPLSAARAM
NKAALTVFQDIIKNGSQKQSIDSMQTRDELYEMLGYHTFEKKLDELFKHKK

Specific function: Catalyzes the formation of pyruvate and succinate from 2-methylisocitrate [H]

COG id: COG2513

COG function: function code G; PEP phosphonomutase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate lyase/PEP mutase superfamily. Methylisocitrate lyase family [H]

Homologues:

Organism=Escherichia coli, GI1786525, Length=292, Percent_Identity=60.2739726027397, Blast_Score=363, Evalue=1e-102,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000918
- InterPro:   IPR018523
- InterPro:   IPR012695
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00463 ICL [H]

EC number: =4.1.3.30 [H]

Molecular weight: Translated: 31866; Mature: 31866

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00161 ISOCITRATE_LYASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSQGIKLREAVAQNNPLQIIGVINAYSAIQAQKSGAKALYLSGGALAAMSLGVPDLGIS
CCCCCCHHHHHHHCCCCEEEEEHHHHHHHHHHHHCCCCEEEECCCHHHHHHCCCCCCCHH
SLEDVCIDVRRITAASDLPLLVDADTGWGGAFNIARTIKDLTRSGAAGCHIEDQVAQKRC
HHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
GHRPNKELVSLEEMCDRIKAGVDAKIDENFVLMARTDAHASEGQNRAIERALAYVEAGAD
CCCCCHHHHHHHHHHHHHHCCCCCEECCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCH
MIFAEAIHTLEEYKQFTQNIKVPVLANITEFGKTPYFTTSELQNVGVKMVLYPLSAARAM
HHHHHHHHHHHHHHHHHHCCCCCCEECHHHHCCCCCEEHHHHHHCCEEEEEEHHHHHHHH
NKAALTVFQDIIKNGSQKQSIDSMQTRDELYEMLGYHTFEKKLDELFKHKK
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKSQGIKLREAVAQNNPLQIIGVINAYSAIQAQKSGAKALYLSGGALAAMSLGVPDLGIS
CCCCCCHHHHHHHCCCCEEEEEHHHHHHHHHHHHCCCCEEEECCCHHHHHHCCCCCCCHH
SLEDVCIDVRRITAASDLPLLVDADTGWGGAFNIARTIKDLTRSGAAGCHIEDQVAQKRC
HHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
GHRPNKELVSLEEMCDRIKAGVDAKIDENFVLMARTDAHASEGQNRAIERALAYVEAGAD
CCCCCHHHHHHHHHHHHHHCCCCCEECCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCH
MIFAEAIHTLEEYKQFTQNIKVPVLANITEFGKTPYFTTSELQNVGVKMVLYPLSAARAM
HHHHHHHHHHHHHHHHHHCCCCCCEECHHHHCCCCCEEHHHHHHCCEEEEEEHHHHHHHH
NKAALTVFQDIIKNGSQKQSIDSMQTRDELYEMLGYHTFEKKLDELFKHKK
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503; 9325432 [H]