Click here to switch to the map view.

The map label for this gene is prpD

Identifier: 32265898

GI number: 32265898

Start: 386603

End: 388060

Strand: Direct

Name: prpD

Synonym: HH0399

Alternate gene names: 32265898

Gene position: 386603-388060 (Clockwise)

Preceding gene: 32265897

Following gene: 32265899

Centisome position: 21.49

GC content: 41.98

Gene sequence:

>1458_bases
ATGAGTGTAGATATGGGTATTTTAGAGACACAACGACCGGAGTTTGATGAGCTATTAAGTAAAATTGCTCGCTATGCGTT
AGAGTTTGAGATTAACTCACCTCTTGCATACGAGACTGCAAGATATTGCCTTATGGATACCATAGGTTGTGGGCTTTTAG
CACTGAATTTTCCTGCTTGCACGAAGCTTTTAGGACCTGTGGTAGAGGGTGCAGAATTTCGTCCGCTAGGGGCAAAGATT
CCGGGCACAAGCTATCAGTTAGAGCCAGAAAGAGCAGCATTTAATGTTGGGGCAATGGTGCGGTGGCTTGATTTTAACGA
TACTTGGCTTGCCGCAGAATGGGGACATCCAAGCGATAATTTGGGAGCAATTTGGGCAGTGGCTGATTATCTTAGTCGTA
AAAATATCGCACAAGGCAAAGAACCATTAAGGGTAAAAGATGTCCTAAGTGCAATGATTAAGGCACACGAGATTCAAGGA
ATCTTAGCCTTAGAAAATTGTTTTAATAAAGTAGGACTAGACCACGTGCTACTTGTGAGAATCGCATCTACTGCGGTAGC
TTGTGCTATGCTTGGAGGTAATTTTGAGGAAGTGCGTAATGCCATAAGCCACGCTTTTATTGATGGTGGAGCTTTAAGAA
CTTATCGCCACGCTCCAAATACAGGCAGTCGTAAAAGTTGGGCGGCAGGTGATGCAAGCTCAAGAGGCGTGAATCTCGCC
TTAAAAGCACTCAGTGGCGAAATGGGATATCCAAGTGCATTGAGTGCATCATTTTGGGGATTTGAAGATGTGAAAATGAA
AGGGCAAAAGCTCACTATTCCACAAGAATTTGGTAGCTATGTAATGGAAAATGTGCTTTTTAAAATTAGCTTCCCTGCGG
AATTTCACGCACAAACTGCGGTAGAATGCGCAATTAAGCTTCATAACGAAGTGAAAAATCGCCTTGATGAAATTGAACGC
ATAGTGATTACTACACAAGAATCCGGACATAGAATCATCAATAAAGTAGGACCTTTGGCTAATCCAGCTGATAGAGACCA
TTGTATTCAATATATGGTGGCTGTTCCGCTTGTGTTTGGGCGATTGACCGCAGAGGATTATGAAGATAGTGTAGCCTCTG
ACCCACGCATTGACTCATTACGAGATAAGATGAGTGTAGAAGTTGATGACCGCTATACACGCGAATATTTAGAATCTGAT
AAGCGTAGCATCGCTAATGCAGTGCAGATTTTCTACAAAGATGGTAGCAAAAGTCAAAAAGTAGAAGTAGAGTATCCTAT
CGGGCATAAACGCCGTAGAGATGAGGGAATTCCTGTGCTTGTAGATAAATTTAAACGCAATATTGCCCTCAAGCTTAGCT
CTAAAAAATGTGCATTGATTGAAAAGCTTTGCAGCGACCAAAAAACTTTAGAATCTACCCCGTTTAACGAGTTTAGCGAT
TTATTTGCACTCTCTTAA

Upstream 100 bases:

>100_bases
TATTCGTCCAAGTTCAGAATATATTGGACCAGATAAAAAGGCATTTGTGCCATTGCATAATCGCTAGGCACATTTTTAAT
CTAAATTAAAGGAGAACACA

Downstream 100 bases:

>100_bases
AGGGGTGCATTGCCAAGAAATACAGATTCTATAAAAGGAATGTGTAAATGGAAAAAATGATTATTTTAAACATAGAGCAT
AAAGAACATATGTTTAATCA

Product: 2-methylcitrate dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 485; Mature: 484

Protein sequence:

>485_residues
MSVDMGILETQRPEFDELLSKIARYALEFEINSPLAYETARYCLMDTIGCGLLALNFPACTKLLGPVVEGAEFRPLGAKI
PGTSYQLEPERAAFNVGAMVRWLDFNDTWLAAEWGHPSDNLGAIWAVADYLSRKNIAQGKEPLRVKDVLSAMIKAHEIQG
ILALENCFNKVGLDHVLLVRIASTAVACAMLGGNFEEVRNAISHAFIDGGALRTYRHAPNTGSRKSWAAGDASSRGVNLA
LKALSGEMGYPSALSASFWGFEDVKMKGQKLTIPQEFGSYVMENVLFKISFPAEFHAQTAVECAIKLHNEVKNRLDEIER
IVITTQESGHRIINKVGPLANPADRDHCIQYMVAVPLVFGRLTAEDYEDSVASDPRIDSLRDKMSVEVDDRYTREYLESD
KRSIANAVQIFYKDGSKSQKVEVEYPIGHKRRRDEGIPVLVDKFKRNIALKLSSKKCALIEKLCSDQKTLESTPFNEFSD
LFALS

Sequences:

>Translated_485_residues
MSVDMGILETQRPEFDELLSKIARYALEFEINSPLAYETARYCLMDTIGCGLLALNFPACTKLLGPVVEGAEFRPLGAKI
PGTSYQLEPERAAFNVGAMVRWLDFNDTWLAAEWGHPSDNLGAIWAVADYLSRKNIAQGKEPLRVKDVLSAMIKAHEIQG
ILALENCFNKVGLDHVLLVRIASTAVACAMLGGNFEEVRNAISHAFIDGGALRTYRHAPNTGSRKSWAAGDASSRGVNLA
LKALSGEMGYPSALSASFWGFEDVKMKGQKLTIPQEFGSYVMENVLFKISFPAEFHAQTAVECAIKLHNEVKNRLDEIER
IVITTQESGHRIINKVGPLANPADRDHCIQYMVAVPLVFGRLTAEDYEDSVASDPRIDSLRDKMSVEVDDRYTREYLESD
KRSIANAVQIFYKDGSKSQKVEVEYPIGHKRRRDEGIPVLVDKFKRNIALKLSSKKCALIEKLCSDQKTLESTPFNEFSD
LFALS
>Mature_484_residues
SVDMGILETQRPEFDELLSKIARYALEFEINSPLAYETARYCLMDTIGCGLLALNFPACTKLLGPVVEGAEFRPLGAKIP
GTSYQLEPERAAFNVGAMVRWLDFNDTWLAAEWGHPSDNLGAIWAVADYLSRKNIAQGKEPLRVKDVLSAMIKAHEIQGI
LALENCFNKVGLDHVLLVRIASTAVACAMLGGNFEEVRNAISHAFIDGGALRTYRHAPNTGSRKSWAAGDASSRGVNLAL
KALSGEMGYPSALSASFWGFEDVKMKGQKLTIPQEFGSYVMENVLFKISFPAEFHAQTAVECAIKLHNEVKNRLDEIERI
VITTQESGHRIINKVGPLANPADRDHCIQYMVAVPLVFGRLTAEDYEDSVASDPRIDSLRDKMSVEVDDRYTREYLESDK
RSIANAVQIFYKDGSKSQKVEVEYPIGHKRRRDEGIPVLVDKFKRNIALKLSSKKCALIEKLCSDQKTLESTPFNEFSDL
FALS

Specific function: Catalyzes the dehydration of 2-methylcitrate to 2- methyl-cis-aconitate [H]

COG id: COG2079

COG function: function code R; Uncharacterized protein involved in propionate catabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prpD family [H]

Homologues:

Organism=Escherichia coli, GI1786528, Length=476, Percent_Identity=60.2941176470588, Blast_Score=608, Evalue=1e-175,
Organism=Saccharomyces cerevisiae, GI6325258, Length=489, Percent_Identity=54.3967280163599, Blast_Score=508, Evalue=1e-145,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012705
- InterPro:   IPR005656 [H]

Pfam domain/function: PF03972 MmgE_PrpD [H]

EC number: =4.2.1.79 [H]

Molecular weight: Translated: 53937; Mature: 53806

Theoretical pI: Translated: 6.21; Mature: 6.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVDMGILETQRPEFDELLSKIARYALEFEINSPLAYETARYCLMDTIGCGLLALNFPAC
CCCCCCCCCCCCCCHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHCCHHEEECCHHH
TKLLGPVVEGAEFRPLGAKIPGTSYQLEPERAAFNVGAMVRWLDFNDTWLAAEWGHPSDN
HHHHHHHHCCCCCCCCCCCCCCCCEEECHHHHHCCCCCEEEEEECCCCEEEEECCCCCCC
LGAIWAVADYLSRKNIAQGKEPLRVKDVLSAMIKAHEIQGILALENCFNKVGLDHVLLVR
CHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
IASTAVACAMLGGNFEEVRNAISHAFIDGGALRTYRHAPNTGSRKSWAAGDASSRGVNLA
HHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCEEE
LKALSGEMGYPSALSASFWGFEDVKMKGQKLTIPQEFGSYVMENVLFKISFPAEFHAQTA
EEECCCCCCCCCCCCCCCCCCHHHHCCCCEEECHHHHHHHHHHHHHEEEECCHHHHHHHH
VECAIKLHNEVKNRLDEIERIVITTQESGHRIINKVGPLANPADRDHCIQYMVAVPLVFG
HHHHHHHHHHHHHHHHHHHEEEEEECCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
RLTAEDYEDSVASDPRIDSLRDKMSVEVDDRYTREYLESDKRSIANAVQIFYKDGSKSQK
HHHHHHHHHHHCCCCCHHHHHHHHCEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCE
VEVEYPIGHKRRRDEGIPVLVDKFKRNIALKLSSKKCALIEKLCSDQKTLESTPFNEFSD
EEEECCCCCCCCCCCCCCHHHHHHCCCEEEEECCCHHHHHHHHHCCCHHHHCCCCHHHHH
LFALS
HHCCC
>Mature Secondary Structure 
SVDMGILETQRPEFDELLSKIARYALEFEINSPLAYETARYCLMDTIGCGLLALNFPAC
CCCCCCCCCCCCCHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHCCHHEEECCHHH
TKLLGPVVEGAEFRPLGAKIPGTSYQLEPERAAFNVGAMVRWLDFNDTWLAAEWGHPSDN
HHHHHHHHCCCCCCCCCCCCCCCCEEECHHHHHCCCCCEEEEEECCCCEEEEECCCCCCC
LGAIWAVADYLSRKNIAQGKEPLRVKDVLSAMIKAHEIQGILALENCFNKVGLDHVLLVR
CHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
IASTAVACAMLGGNFEEVRNAISHAFIDGGALRTYRHAPNTGSRKSWAAGDASSRGVNLA
HHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCEEE
LKALSGEMGYPSALSASFWGFEDVKMKGQKLTIPQEFGSYVMENVLFKISFPAEFHAQTA
EEECCCCCCCCCCCCCCCCCCHHHHCCCCEEECHHHHHHHHHHHHHEEEECCHHHHHHHH
VECAIKLHNEVKNRLDEIERIVITTQESGHRIINKVGPLANPADRDHCIQYMVAVPLVFG
HHHHHHHHHHHHHHHHHHHEEEEEECCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
RLTAEDYEDSVASDPRIDSLRDKMSVEVDDRYTREYLESDKRSIANAVQIFYKDGSKSQK
HHHHHHHHHHHCCCCCHHHHHHHHCEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCE
VEVEYPIGHKRRRDEGIPVLVDKFKRNIALKLSSKKCALIEKLCSDQKTLESTPFNEFSD
EEEECCCCCCCCCCCCCCHHHHHHCCCEEEEECCCHHHHHHHHHCCCHHHHCCCCHHHHH
LFALS
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377; 8759838 [H]