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The map label for this gene is pykA

Identifier: 304570489

GI number: 304570489

Start: 1397604

End: 1399031

Strand: Reverse

Name: pykA

Synonym: NA

Alternate gene names: 304570489

Gene position: 1399031-1397604 (Counterclockwise)

Preceding gene: 45657022

Following gene: 45657015

Centisome position: 32.71

GC content: 39.5

Gene sequence:

>1428_bases
ATGAAAATTCTAAACAGAAAAAAAACCAAAATCGTCTGCACTATAGGTCCGGCTTCTTCTTCCGAAGAAACCATACTTTC
TATTCTCAAAGCGGGAATGGATATAGCTCGGATGAATTTTTCACACGGCACACATGATTCTCATAAAAGAGTCTACGACA
CGTTACGCAAGTGCGAACAAATTTTCGGATTTCCTTTAGGGATCATGGCAGACCTACAAGGCCCTAAAATTCGAACCGGA
AAATTAAAACTCAACTCCATTCTACTTCATAAAAATCAGGAGATTGAAATCGTCCCAGATTCTGATATTTTGGGAGATGA
ACACAAAATCGGTTGTACATATCCAAATCTAATTAGAGATATACAAGAAGAAGACAAGATTCTAATTGATGACGGTAAGT
TAATTTTAAAAGTTATTTCTAAAAAATCGAACTCCGCCATTTTAAAAGTAATCGTAGGTGGAATCTTATGGAGCAACAAA
GGAATCAATCTTCCAGGAACTCCTATCTCTGCTCCTGCACTTTCCGAAAAAGACATAGAAGATCTTAAGTTCGCACTCTC
TCTAGGTGTAGATTACGCGGCTCTTAGTTTTGTTCGTACTGGGGCAGACTTAGAATTAGCAAGGTCTTATTTAGAAGGTA
CATATACAGGGCTGATCGCAAAGATAGAAAGACCGGAAGCAATTGGAAACATAGAAGAAATCATAGAACGAGCAGATGGC
ATCATGATTGCAAGAGGAGATTTAGGAGTAGAAATTGATACGGAAAAGGTTCCCATTCTACAAAAAGAACTTATCTATAA
ACTCAATCAAGCGGGTAAACCGGTCATTACTGCAACCCAAATGCTGGAATCTATGATTGAAAACCCAAGACCAACACGTG
CAGAGGCGAGCGACGTAGCAAATGCGGTTATGGACGGAACGGATGCAGTTATGCTTTCTGCAGAATCGGCTAACGGCCAT
TATCCGGTCGAATCCGTTGAAATCATGTCTAAAATCATTCAAGAAACTGAAACCATTGATCATATCTATGAGATTCACTG
GAACATTAAAAAAACTTTCTTAGAATCAGAAAGAACTGCACTGGGAAATGCCGCAAGGGAGATTGCCCACGGAATCCACG
CAAAGGCAATCGTCAATTTTACGAGAAGCGGTTATTCCGCATTGATTACCTCCGAAATGAGACCCAAGGTTCCGATCTAC
TCTTTTACTCCGTTTGCAACCACCGCCAGAAAGATGAAACTCTATCGAGGTGTGGTGCCATTTGTAATGCCTTTCTTTAC
AAGATTGGAAGATATGATTGCTTATATGAATCAAAAACTCAAAGAAGATGAATTTCTTTTTCCAGGAGATAAGGTGGTAA
TTCTTTCCGGAGCCCCAGGAACCACAGTCAGAAGTGTAGACTTTTTACAGGTTTATAAAATACATTGA

Upstream 100 bases:
NA

Downstream 100 bases:
NA

Product: pyruvate kinase

Products: NA

Alternate protein names: PK 1 [H]

Number of amino acids: Translated: 475; Mature: 475

Protein sequence:
NA

Sequences:

>Translated_475_residues
MKILNRKKTKIVCTIGPASSSEETILSILKAGMDIARMNFSHGTHDSHKRVYDTLRKCEQIFGFPLGIMADLQGPKIRTG
KLKLNSILLHKNQEIEIVPDSDILGDEHKIGCTYPNLIRDIQEEDKILIDDGKLILKVISKKSNSAILKVIVGGILWSNK
GINLPGTPISAPALSEKDIEDLKFALSLGVDYAALSFVRTGADLELARSYLEGTYTGLIAKIERPEAIGNIEEIIERADG
IMIARGDLGVEIDTEKVPILQKELIYKLNQAGKPVITATQMLESMIENPRPTRAEASDVANAVMDGTDAVMLSAESANGH
YPVESVEIMSKIIQETETIDHIYEIHWNIKKTFLESERTALGNAAREIAHGIHAKAIVNFTRSGYSALITSEMRPKVPIY
SFTPFATTARKMKLYRGVVPFVMPFFTRLEDMIAYMNQKLKEDEFLFPGDKVVILSGAPGTTVRSVDFLQVYKIH
>Mature_475_residues
MKILNRKKTKIVCTIGPASSSEETILSILKAGMDIARMNFSHGTHDSHKRVYDTLRKCEQIFGFPLGIMADLQGPKIRTG
KLKLNSILLHKNQEIEIVPDSDILGDEHKIGCTYPNLIRDIQEEDKILIDDGKLILKVISKKSNSAILKVIVGGILWSNK
GINLPGTPISAPALSEKDIEDLKFALSLGVDYAALSFVRTGADLELARSYLEGTYTGLIAKIERPEAIGNIEEIIERADG
IMIARGDLGVEIDTEKVPILQKELIYKLNQAGKPVITATQMLESMIENPRPTRAEASDVANAVMDGTDAVMLSAESANGH
YPVESVEIMSKIIQETETIDHIYEIHWNIKKTFLESERTALGNAAREIAHGIHAKAIVNFTRSGYSALITSEMRPKVPIY
SFTPFATTARKMKLYRGVVPFVMPFFTRLEDMIAYMNQKLKEDEFLFPGDKVVILSGAPGTTVRSVDFLQVYKIH

Specific function: Glycolysis; final step. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286418, Length=479, Percent_Identity=40.5010438413361, Blast_Score=327, Evalue=2e-89,
Organism=Homo sapiens, GI33286422, Length=479, Percent_Identity=40.0835073068894, Blast_Score=322, Evalue=4e-88,
Organism=Homo sapiens, GI33286420, Length=479, Percent_Identity=40.0835073068894, Blast_Score=322, Evalue=4e-88,
Organism=Homo sapiens, GI10835121, Length=484, Percent_Identity=39.8760330578512, Blast_Score=318, Evalue=8e-87,
Organism=Homo sapiens, GI32967597, Length=484, Percent_Identity=39.8760330578512, Blast_Score=318, Evalue=9e-87,
Organism=Homo sapiens, GI310128732, Length=287, Percent_Identity=41.1149825783972, Blast_Score=207, Evalue=2e-53,
Organism=Homo sapiens, GI310128730, Length=287, Percent_Identity=41.1149825783972, Blast_Score=207, Evalue=2e-53,
Organism=Homo sapiens, GI310128736, Length=235, Percent_Identity=37.4468085106383, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI310128734, Length=235, Percent_Identity=37.4468085106383, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI310128738, Length=214, Percent_Identity=35.5140186915888, Blast_Score=130, Evalue=4e-30,
Organism=Escherichia coli, GI1787965, Length=464, Percent_Identity=42.0258620689655, Blast_Score=333, Evalue=2e-92,
Organism=Escherichia coli, GI1788160, Length=480, Percent_Identity=38.9583333333333, Blast_Score=312, Evalue=4e-86,
Organism=Caenorhabditis elegans, GI17544584, Length=467, Percent_Identity=40.8993576017131, Blast_Score=334, Evalue=6e-92,
Organism=Caenorhabditis elegans, GI17506829, Length=472, Percent_Identity=39.4067796610169, Blast_Score=290, Evalue=8e-79,
Organism=Caenorhabditis elegans, GI71984413, Length=472, Percent_Identity=39.4067796610169, Blast_Score=290, Evalue=9e-79,
Organism=Caenorhabditis elegans, GI71984406, Length=472, Percent_Identity=39.4067796610169, Blast_Score=290, Evalue=1e-78,
Organism=Caenorhabditis elegans, GI17506831, Length=472, Percent_Identity=39.4067796610169, Blast_Score=290, Evalue=2e-78,
Organism=Saccharomyces cerevisiae, GI6319279, Length=468, Percent_Identity=40.1709401709402, Blast_Score=299, Evalue=8e-82,
Organism=Saccharomyces cerevisiae, GI6324923, Length=447, Percent_Identity=41.1633109619687, Blast_Score=285, Evalue=1e-77,
Organism=Drosophila melanogaster, GI24648964, Length=471, Percent_Identity=41.8259023354565, Blast_Score=321, Evalue=7e-88,
Organism=Drosophila melanogaster, GI28571814, Length=471, Percent_Identity=41.8259023354565, Blast_Score=320, Evalue=1e-87,
Organism=Drosophila melanogaster, GI24648966, Length=408, Percent_Identity=39.9509803921569, Blast_Score=278, Evalue=8e-75,
Organism=Drosophila melanogaster, GI24581235, Length=477, Percent_Identity=32.2851153039832, Blast_Score=251, Evalue=1e-66,
Organism=Drosophila melanogaster, GI24646914, Length=269, Percent_Identity=40.1486988847584, Blast_Score=189, Evalue=4e-48,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 52698; Mature: 52698

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: PS00307 LECTIN_LEGUME_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILNRKKTKIVCTIGPASSSEETILSILKAGMDIARMNFSHGTHDSHKRVYDTLRKCEQ
CCCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHHHH
IFGFPLGIMADLQGPKIRTGKLKLNSILLHKNQEIEIVPDSDILGDEHKIGCTYPNLIRD
HHCCCHHHHCCCCCCCEEECCEEEEEEEEECCCEEEEECCCCCCCCCCCCCCCCHHHHHH
IQEEDKILIDDGKLILKVISKKSNSAILKVIVGGILWSNKGINLPGTPISAPALSEKDIE
HHHCCCEEEECCHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHH
DLKFALSLGVDYAALSFVRTGADLELARSYLEGTYTGLIAKIERPEAIGNIEEIIERADG
HHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHEEEECCCHHCCCHHHHHHHCCC
IMIARGDLGVEIDTEKVPILQKELIYKLNQAGKPVITATQMLESMIENPRPTRAEASDVA
EEEEECCCCEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHH
NAVMDGTDAVMLSAESANGHYPVESVEIMSKIIQETETIDHIYEIHWNIKKTFLESERTA
HHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGNAAREIAHGIHAKAIVNFTRSGYSALITSEMRPKVPIYSFTPFATTARKMKLYRGVVP
HHHHHHHHHHHHHHHHHHHHHHCCHHHEEECCCCCCCCEEECCCHHHHHHHHHHHHHHHH
FVMPFFTRLEDMIAYMNQKLKEDEFLFPGDKVVILSGAPGTTVRSVDFLQVYKIH
HHHHHHHHHHHHHHHHHHHCCCCCEECCCCEEEEEECCCCCCEECCCEEEEEEEC
>Mature Secondary Structure
MKILNRKKTKIVCTIGPASSSEETILSILKAGMDIARMNFSHGTHDSHKRVYDTLRKCEQ
CCCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHHHH
IFGFPLGIMADLQGPKIRTGKLKLNSILLHKNQEIEIVPDSDILGDEHKIGCTYPNLIRD
HHCCCHHHHCCCCCCCEEECCEEEEEEEEECCCEEEEECCCCCCCCCCCCCCCCHHHHHH
IQEEDKILIDDGKLILKVISKKSNSAILKVIVGGILWSNKGINLPGTPISAPALSEKDIE
HHHCCCEEEECCHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHH
DLKFALSLGVDYAALSFVRTGADLELARSYLEGTYTGLIAKIERPEAIGNIEEIIERADG
HHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHEEEECCCHHCCCHHHHHHHCCC
IMIARGDLGVEIDTEKVPILQKELIYKLNQAGKPVITATQMLESMIENPRPTRAEASDVA
EEEEECCCCEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHH
NAVMDGTDAVMLSAESANGHYPVESVEIMSKIIQETETIDHIYEIHWNIKKTFLESERTA
HHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGNAAREIAHGIHAKAIVNFTRSGYSALITSEMRPKVPIYSFTPFATTARKMKLYRGVVP
HHHHHHHHHHHHHHHHHHHHHHCCHHHEEECCCCCCCCEEECCCHHHHHHHHHHHHHHHH
FVMPFFTRLEDMIAYMNQKLKEDEFLFPGDKVVILSGAPGTTVRSVDFLQVYKIH
HHHHHHHHHHHHHHHHHHHCCCCCEECCCCEEEEEECCCCCCEECCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]