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The map label for this gene is hpaI

Identifier: 30065590

GI number: 30065590

Start: 4543581

End: 4544384

Strand: Reverse

Name: hpaI

Synonym: S4649

Alternate gene names: 30065590

Gene position: 4544384-4543581 (Counterclockwise)

Preceding gene: 30065591

Following gene: 30065589

Centisome position: 98.8

GC content: 58.33

Gene sequence:

>804_bases
ATGGAAAACAGTTTTAAAGCGGCGCTGAAAGCAGGCCGTCCGCAGATTGGATTATGGCTGGGGCTGAGCAGCAGCTACAG
CGCGGAGTTACTGGCCGGACCAGGATTCGACTGGTTATTGATCGACGGTGAGCACGCGCCGAATAACGTGCAAACCGTGC
TCACCCAACTACAGGCGATTGCGCCCTACCCCAGCCAGCCAGTGGTACGCCCATCGTGGAACGATCCGGTGCAAATCAAA
CAACTGCTGGACGTAGGCACACAAACCTTACTGGTGCCGATGGTACAAAACGCCGACGAAGCCCGTAAAGCGGTACGCGC
CACCCGTTATCCCCCCGCCGGTATTCGCGGTGTGGGCAGTGCGCTGGCTCGCGCCTCGCGCTGGAATCGCATTCCTGATT
ACCTGCAAAAAGCCAACGATCAAATGTGCGTGCTGGTGCAGATCGAAACGCGTGAGGCAATGAAGAACTTACCGCAGATT
CTGGACGTGGAAGGGGTCGACGGCGTGTTTATCGGCCCGGCGGATCTGAGCGCCGATATGGGTTATGCCGGTAATCCGCA
GCACCCGGAAGTACAGGCCGCCATTGAGCAGGCGATCGTGCAGATCCGCGAAGCGGGCAAAGCGCCGGGGATCCTGATCG
CCAATGAGCAACTGGCAAAACGCTATCTGGAACTGGGCGCGCTGTTTGTCGCCGTCGGCGTTGACACCACCCTGCTCGCC
CGCGCCGCCGAAGCGCTGGCAGCACGGTTTGGTAATAGTTCTTCTATTTCGATGGCGAAGCAAAACAACAACTCTGTCTA
CTAA

Upstream 100 bases:

>100_bases
TCGTTCACCCTCCCGGTTCCGGCGCGTAAGGGCGACACCTTCCACGTCGATTACGGCAACATGGGCTCCATTAGCTGCCG
CTTTGTTTAAGGAGAGAACG

Downstream 100 bases:

>100_bases
ACGTACGATGTGGAGCGCATCATGACGACCTCTACCCTGCAATATAAAGATAATAAAACTGTTGAAATTGAACATCGTGT
CATTAATAAATTATTTCGTC

Product: 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase

Products: NA

Alternate protein names: 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase; HHED aldolase; 4-hydroxy-2-ketoheptane-1,7-dioate aldolase; HKHD aldolase

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGPGFDWLLIDGEHAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIK
QLLDVGTQTLLVPMVQNADEARKAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQLAKRYLELGALFVAVGVDTTLLA
RAAEALAARFGNSSSISMAKQNNNSVY

Sequences:

>Translated_267_residues
MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGPGFDWLLIDGEHAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIK
QLLDVGTQTLLVPMVQNADEARKAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQLAKRYLELGALFVAVGVDTTLLA
RAAEALAARFGNSSSISMAKQNNNSVY
>Mature_267_residues
MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGPGFDWLLIDGEHAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIK
QLLDVGTQTLLVPMVQNADEARKAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQLAKRYLELGALFVAVGVDTTLLA
RAAEALAARFGNSSSISMAKQNNNSVY

Specific function: Catalyzes the reversible retro-aldol cleavage of 4- hydroxy-2-ketoheptane-1,7-dioate (HKHD) to pyruvate and succinic semialdehyde

COG id: COG3836

COG function: function code G; 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family

Homologues:

Organism=Escherichia coli, GI1788578, Length=250, Percent_Identity=56.4, Blast_Score=291, Evalue=4e-80,
Organism=Escherichia coli, GI1789514, Length=248, Percent_Identity=47.9838709677419, Blast_Score=232, Evalue=2e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HPCH_SHIFL (Q83P19)

Other databases:

- EMBL:   AE005674
- EMBL:   AE014073
- RefSeq:   NP_710088.1
- RefSeq:   NP_839761.1
- HSSP:   P23522
- ProteinModelPortal:   Q83P19
- EnsemblBacteria:   EBESCT00000087796
- EnsemblBacteria:   EBESCT00000092638
- GeneID:   1023444
- GeneID:   1080846
- GenomeReviews:   AE005674_GR
- GenomeReviews:   AE014073_GR
- KEGG:   sfl:SF4379
- KEGG:   sfx:S4649
- GeneTree:   EBGT00050000008793
- HOGENOM:   HBG747616
- OMA:   DYLHRAN
- ProtClustDB:   CLSK2519692
- BioCyc:   SFLE198214:AAN45795.1-MONOMER
- HAMAP:   MF_01292
- InterPro:   IPR005000
- InterPro:   IPR012689
- InterPro:   IPR015813
- Gene3D:   G3DSA:3.20.20.60
- TIGRFAMs:   TIGR02311

Pfam domain/function: PF03328 HpcH_HpaI; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: 4.1.2.-

Molecular weight: Translated: 28722; Mature: 28722

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: NA

Important sites: ACT_SITE 45-45 BINDING 147-147 BINDING 174-174 BINDING 175-175

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGPGFDWLLIDGEHAPNNVQTVLTQLQAI
CCCHHHHHHHCCCCCEEEEEECCCCCCCCEEECCCCCEEEEECCCCCCHHHHHHHHHHHH
APYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEARKAVRATRYPPAGIRGVGS
CCCCCCCCCCCCCCCHHHHHHHHHHCHHHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHH
ALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQILDVEGVDGVFIGPADLSADM
HHHHHHHCCCCHHHHHHCCCCEEEEEEECHHHHHHCCHHHCCCCCCCEEEECCCCCCCCC
GYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQLAKRYLELGALFVAVGVDTTLLA
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHHHHCCCHHHHH
RAAEALAARFGNSSSISMAKQNNNSVY
HHHHHHHHHCCCCCCEEEEECCCCCCC
>Mature Secondary Structure
MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGPGFDWLLIDGEHAPNNVQTVLTQLQAI
CCCHHHHHHHCCCCCEEEEEECCCCCCCCEEECCCCCEEEEECCCCCCHHHHHHHHHHHH
APYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEARKAVRATRYPPAGIRGVGS
CCCCCCCCCCCCCCCHHHHHHHHHHCHHHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHH
ALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQILDVEGVDGVFIGPADLSADM
HHHHHHHCCCCHHHHHHCCCCEEEEEEECHHHHHHCCHHHCCCCCCCEEEECCCCCCCCC
GYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQLAKRYLELGALFVAVGVDTTLLA
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHHHHCCCHHHHH
RAAEALAARFGNSSSISMAKQNNNSVY
HHHHHHHHHCCCCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12384590; 12704152